FastQCFastQC Report
Thu 26 May 2016
SRR1364347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758787
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29900.052.3678671
GGTATCA20650.049.5893821
ATCAACG35250.043.5629583
TCAACGC35550.043.3615264
CAACGCA36500.041.9056245
AACGCAG37100.041.2255636
TATCAAC38850.039.6794362
GTACATG83550.037.1966971
TACATGG86850.035.087862
ACATGGG85550.034.99493
ACGCAGA45200.033.8272137
CGCAGAG45400.033.4161078
CATGGGG49900.031.846024
GCAGAGT52700.028.222859
ATGGGGA29150.025.1129995
CATGGGA41300.024.7869344
ATGGGGG25950.023.622835
CAGAGTA51100.023.28523410-11
GTATAGG4400.021.6494851
AGAGTAC52800.021.23972910-11
Produced by FastQC (version 0.11.5)