FastQCFastQC Report
Thu 26 May 2016
SRR1364347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758787
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.15379918091275407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.14174541885970274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.063.3681531
GTATCAA46750.052.8697051
ATCAACG58650.041.2192043
TCAACGC59600.040.4622764
TATCAAC61050.039.891392
CAACGCA61250.039.0806275
AACGCAG62650.038.0905956
GTACATG83000.035.5913121
ACATGGG84750.032.951463
TACATGG87400.032.7699052
ACGCAGA75550.031.5795257
CGCAGAG77300.030.7106558
CATGGGG48000.030.5165484
GTGGTAT15500.029.2026521
TGGTATC15250.027.3318962
GCAGAGT87000.026.876279
GAGTACT44350.026.2940912-13
ATGGGGA28750.025.4746825
CAGAGTA83300.024.85592510-11
GTACTTT48800.023.95801214-15
Produced by FastQC (version 0.11.5)