##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364347_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1758787 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.268753407888504 33.0 31.0 34.0 30.0 34.0 2 32.303118001213335 33.0 31.0 34.0 30.0 34.0 3 32.41153135655426 34.0 31.0 34.0 30.0 34.0 4 36.013472921962695 37.0 35.0 37.0 35.0 37.0 5 35.95107707755402 37.0 35.0 37.0 35.0 37.0 6 36.01076651123758 37.0 35.0 37.0 35.0 37.0 7 35.96505318722506 37.0 35.0 37.0 35.0 37.0 8 35.97317071367937 37.0 35.0 37.0 35.0 37.0 9 37.725114524953845 39.0 38.0 39.0 35.0 39.0 10-11 37.67764345540421 39.0 37.5 39.0 35.0 39.0 12-13 37.65034196864089 39.0 37.0 39.0 35.0 39.0 14-15 39.048166719449256 41.0 38.0 41.0 35.0 41.0 16-17 39.04315815388674 41.0 38.0 41.0 35.0 41.0 18-19 39.09184824541005 41.0 38.5 41.0 35.0 41.0 20-21 39.10053036553033 41.0 39.0 41.0 35.0 41.0 22-23 39.06122941550057 41.0 39.0 41.0 35.0 41.0 24-25 39.027313142523795 41.0 39.0 41.0 35.0 41.0 26-27 38.93452220194941 41.0 38.0 41.0 35.0 41.0 28-29 38.845856547722946 40.0 38.0 41.0 35.0 41.0 30-31 38.74538787243708 40.0 38.0 41.0 35.0 41.0 32-33 38.64098751014193 40.0 38.0 41.0 34.0 41.0 34-35 38.551021812192154 40.0 38.0 41.0 34.0 41.0 36-37 38.438443938919264 40.0 38.0 41.0 34.0 41.0 38-39 38.35463504108229 40.0 38.0 41.0 34.0 41.0 40-41 38.24765107997728 40.0 38.0 41.0 33.5 41.0 42-43 38.10164476994656 40.0 38.0 41.0 33.0 41.0 44-45 37.945105916748304 40.0 37.5 41.0 33.0 41.0 46-47 37.73375656062957 40.0 37.0 41.0 33.0 41.0 48-49 37.53805492080622 40.0 37.0 41.0 32.5 41.0 50-51 37.37767961669037 40.0 37.0 41.0 32.0 41.0 52-53 37.22073394902282 40.0 36.0 41.0 32.0 41.0 54-55 37.03467190739981 40.0 36.0 41.0 31.0 41.0 56-57 36.83824761042696 39.0 35.5 41.0 31.0 41.0 58-59 36.62285370542311 39.0 35.0 41.0 31.0 41.0 60-61 36.375219398369445 39.0 35.0 41.0 31.0 41.0 62-63 36.14262016946907 38.0 35.0 41.0 31.0 41.0 64-65 35.860909820234056 37.5 35.0 40.0 30.0 41.0 66-67 35.538274105960525 37.0 35.0 40.0 30.0 41.0 68-69 35.20766556723469 36.5 34.5 39.5 29.0 41.0 70-71 34.954705999077774 36.0 34.0 39.0 29.5 41.0 72-73 34.7590251121938 36.0 34.0 39.0 30.0 41.0 74-75 34.423268423066574 35.0 34.0 38.0 29.5 40.0 76-77 34.093769171593834 35.0 34.0 37.0 29.0 39.0 78-79 33.78019595323368 35.0 34.0 37.0 29.0 39.0 80-81 33.480642056144376 35.0 34.0 36.0 29.0 38.5 82-83 33.21278642609935 35.0 34.0 36.0 29.0 37.0 84-85 32.99197941535843 35.0 34.0 35.5 29.0 37.0 86-87 32.804943691305425 35.0 34.0 35.0 29.0 36.5 88-89 32.65876311344125 35.0 34.0 35.0 29.0 36.0 90-91 32.526515433648306 35.0 33.0 35.0 28.5 36.0 92-93 32.40750841346906 35.0 33.0 35.0 27.5 36.0 94-95 32.32905178398521 35.0 33.0 35.0 27.0 35.0 96-97 32.256448904841804 35.0 33.0 35.0 27.0 35.0 98-99 32.18892907441322 35.0 33.0 35.0 27.0 35.0 100-101 32.13040294248252 35.0 33.0 35.0 27.0 35.0 102-103 31.135265953182504 34.0 31.5 35.0 24.5 35.0 104-105 31.87289052056901 35.0 33.0 35.0 26.0 35.0 106-107 32.184516089782335 35.0 33.0 35.0 27.0 35.0 108-109 32.19817578819948 35.0 34.0 35.0 27.0 35.0 110-111 32.16970559823333 35.0 34.0 35.0 27.0 35.0 112-113 32.10809751266072 35.0 33.5 35.0 27.0 35.0 114-115 32.02928154461 35.0 33.0 35.0 27.0 35.0 116-117 31.970128560195178 35.0 33.0 35.0 27.0 35.0 118-119 31.858793588990594 35.0 33.0 35.0 27.0 35.0 120-121 31.723757908149196 35.0 33.0 35.0 26.0 35.0 122-123 31.570341945897937 35.0 33.0 35.0 25.0 35.0 124-125 30.84892883561227 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.062022366670845486 1101 2 0.08024186441966208 1101 3 0.09683790760863786 1101 4 0.0833021086711625 1101 5 0.12641523622590256 1101 6 0.11778186928699341 1101 7 0.21274880169561072 1101 8 0.2005822707940581 1101 9 0.1871131862843427 1101 10-11 0.24083791317575987 1101 12-13 0.17105523672741896 1101 14-15 0.2063979829093796 1101 16-17 0.22872981484204047 1101 18-19 0.2997353634782769 1101 20-21 0.24093579819593458 1101 22-23 0.2788144012302496 1101 24-25 0.28034278551292857 1101 26-27 0.1413222250186763 1101 28-29 0.14683494182659018 1101 30-31 0.211436070069297 1101 32-33 0.1533877662998293 1101 34-35 0.2017745407495255 1101 36-37 0.2752504401122664 1101 38-39 0.23010526407705356 1101 40-41 0.0557490733312207 1101 42-43 0.1828312941169088 1101 44-45 0.11775435702134018 1101 46-47 0.17746415357888878 1101 48-49 -0.006105052476463868 1101 50-51 0.010514803804149153 1101 52-53 0.0023863707087627972 1101 54-55 0.07824446964814769 1101 56-57 0.023809648127304683 1101 58-59 -0.046901027962888975 1101 60-61 0.025698325728356508 1101 62-63 -0.07221420136086465 1101 64-65 -0.17358219367350358 1101 66-67 -0.05205144336130019 1101 68-69 -0.026386135367602037 1101 70-71 -0.06243555172041937 1101 72-73 -0.09354891451849312 1101 74-75 0.04774422025901259 1101 76-77 -0.21030175425584474 1101 78-79 -0.39237684187119015 1101 80-81 -0.3909249048019703 1101 82-83 -0.4221970098208345 1101 84-85 -0.6805017906056179 1101 86-87 -0.5313927793069411 1101 88-89 -0.47403808466145847 1101 90-91 -0.8768832888385134 1101 92-93 -0.7276739700223445 1101 94-95 -0.6658274736218885 1101 96-97 -0.42106822116009823 1101 98-99 -0.4206440622067582 1101 100-101 -0.5274779398024734 1101 102-103 -0.407944060725697 1101 104-105 -0.3557049761021851 1101 106-107 -0.30798272502740076 1101 108-109 -0.24073701325450259 1101 110-111 -0.21891776072308744 1101 112-113 -0.2744969595076512 1101 114-115 -0.3913222684458155 1101 116-117 -0.2956784662544827 1101 118-119 -0.28773087350628757 1101 120-121 -0.17685318124645022 1101 122-123 -0.1856308629111183 1101 124-125 -0.31787754954919123 1102 1 0.20144756614036652 1102 2 0.2606478448334144 1102 3 0.16982539297511323 1102 4 0.18562748914281002 1102 5 0.10391827092733052 1102 6 0.1709282390929019 1102 7 0.27569347725255255 1102 8 0.21933218979669533 1102 9 0.3901176486994018 1102 10-11 0.3187239034387943 1102 12-13 0.322022885298189 1102 14-15 0.44082532070945035 1102 16-17 0.44812468889652735 1102 18-19 0.4926071630022193 1102 20-21 0.41387511712684955 1102 22-23 0.36603560828544346 1102 24-25 0.41502629099267097 1102 26-27 0.4165715889193393 1102 28-29 0.49568110730313464 1102 30-31 0.4578736977751561 1102 32-33 0.4281144271778814 1102 34-35 0.4626078848825088 1102 36-37 0.48810807049643756 1102 38-39 0.4508950208257545 1102 40-41 0.3867036759257374 1102 42-43 0.38330928659171093 1102 44-45 0.34317413938680374 1102 46-47 0.43241924863740877 1102 48-49 0.4554929068218243 1102 50-51 0.33824139124458696 1102 52-53 0.340475653568852 1102 54-55 0.39767455055912393 1102 56-57 0.39332742219000494 1102 58-59 0.3653092714465913 1102 60-61 0.36906431720095156 1102 62-63 0.29345479371018257 1102 64-65 0.3558522502957615 1102 66-67 0.28196788369095316 1102 68-69 0.1828464847568938 1102 70-71 0.3193378802785034 1102 72-73 0.31294426658298136 1102 74-75 0.19472420634906484 1102 76-77 0.24217525329094514 1102 78-79 0.2285152955521852 1102 80-81 0.24509343732227507 1102 82-83 0.28063789810135376 1102 84-85 0.26859816662776836 1102 86-87 0.25176883960367036 1102 88-89 0.25015291361492586 1102 90-91 0.29331011127984397 1102 92-93 0.29066361837278976 1102 94-95 0.19727466082078138 1102 96-97 0.240046993817721 1102 98-99 0.2375878624203338 1102 100-101 0.26818729699063226 1102 102-103 0.3923851125827511 1102 104-105 0.2646676981933531 1102 106-107 0.2287937524726118 1102 108-109 0.3072289808100095 1102 110-111 0.25822845356787383 1102 112-113 0.214436423936057 1102 114-115 0.15888789272815274 1102 116-117 0.11243595818901397 1102 118-119 0.15810629370252016 1102 120-121 0.18634615003927024 1102 122-123 0.08576000231358094 1102 124-125 -0.0195257920142744 1103 1 0.10089486172498141 1103 2 0.12828863527177248 1103 3 0.14091369496484418 1103 4 0.12334580028271347 1103 5 -0.04527299037540189 1103 6 -0.008779410108871843 1103 7 -0.0038882860592437396 1103 8 0.14100132871447357 1103 9 0.18296090797886677 1103 10-11 0.25696877564898557 1103 12-13 0.19140021901798576 1103 14-15 0.31729687845498233 1103 16-17 0.2824121122425325 1103 18-19 0.2129705554990835 1103 20-21 0.18486808115702047 1103 22-23 0.28650513708005576 1103 24-25 0.2769844559571766 1103 26-27 0.23395023548972915 1103 28-29 0.2466907519241488 1103 30-31 0.29404969363768885 1103 32-33 0.33609326243502835 1103 34-35 0.21774007927533034 1103 36-37 0.26749574788755126 1103 38-39 0.27646889566733535 1103 40-41 0.19418735054338043 1103 42-43 0.14432415988173375 1103 44-45 0.17395764407271486 1103 46-47 0.208346769054792 1103 48-49 0.08786874014095503 1103 50-51 -0.012790986719984687 1103 52-53 0.13411287487227241 1103 54-55 0.06349738392939486 1103 56-57 0.04054798677258731 1103 58-59 0.05145315646133497 1103 60-61 0.15740893195602723 1103 62-63 0.13989773585950616 1103 64-65 0.18992228604394512 1103 66-67 0.021608725279335772 1103 68-69 0.05507671101049283 1103 70-71 0.11006850152394776 1103 72-73 0.13609078866558377 1103 74-75 0.1850667978365621 1103 76-77 0.08232418510797146 1103 78-79 0.10674826117368497 1103 80-81 0.11971052256662773 1103 82-83 0.10052033829845186 1103 84-85 0.055892785077020335 1103 86-87 0.1378521970911848 1103 88-89 0.06283513557192322 1103 90-91 0.15493409609397446 1103 92-93 0.0568048606363476 1103 94-95 0.009591444280005135 1103 96-97 0.0085282075509312 1103 98-99 0.0508475113038358 1103 100-101 0.039729271267852084 1103 102-103 0.11627061041455988 1103 104-105 0.19007874546165837 1103 106-107 0.136593278482394 1103 108-109 0.15780228731799895 1103 110-111 0.14992069524604545 1103 112-113 0.07823513722880904 1103 114-115 0.1007888549169138 1103 116-117 0.09316163493224039 1103 118-119 0.07509094301228103 1103 120-121 0.0971757465792571 1103 122-123 0.1496733769079306 1103 124-125 -0.017125577184177843 1104 1 0.19555987456030266 1104 2 0.1770450104923853 1104 3 0.2374047413152809 1104 4 0.21771877435369902 1104 5 0.2687574438042404 1104 6 0.2638768996835523 1104 7 0.23769063950280866 1104 8 0.20277098004608263 1104 9 0.3388545091937516 1104 10-11 0.23632796491484953 1104 12-13 0.2866428356162629 1104 14-15 0.30502046074344946 1104 16-17 0.39207274018054505 1104 18-19 0.2725818533423734 1104 20-21 0.26951695561372446 1104 22-23 0.34022422916463313 1104 24-25 0.30265502299575076 1104 26-27 0.23672855994105646 1104 28-29 0.41389617975949733 1104 30-31 0.391496771322295 1104 32-33 0.2849064917047386 1104 34-35 0.38885472650113684 1104 36-37 0.4145549606891592 1104 38-39 0.36534794443234375 1104 40-41 0.3132845709673333 1104 42-43 0.3539383637265203 1104 44-45 0.34133599338331067 1104 46-47 0.3253555526601062 1104 48-49 0.3360178905239408 1104 50-51 0.37974795513869 1104 52-53 0.3935486041894549 1104 54-55 0.3249269611550929 1104 56-57 0.4205689675279558 1104 58-59 0.3435742814809757 1104 60-61 0.46816335296256284 1104 62-63 0.2730815827494837 1104 64-65 0.22308199298088738 1104 66-67 0.2621969488952871 1104 68-69 0.2643067328256521 1104 70-71 0.3053410844643949 1104 72-73 0.3780680666110996 1104 74-75 0.38487367821230833 1104 76-77 0.4021261252811641 1104 78-79 0.40276889493520684 1104 80-81 0.4556568116027151 1104 82-83 0.3662290746596639 1104 84-85 0.48287000260071977 1104 86-87 0.45432877961040674 1104 88-89 0.4497402468545175 1104 90-91 0.4436307429135198 1104 92-93 0.4574596029142981 1104 94-95 0.5073731724934802 1104 96-97 0.4259438889104601 1104 98-99 0.49481901119767 1104 100-101 0.42987831461791615 1104 102-103 0.4481843907887466 1104 104-105 0.4192903328692914 1104 106-107 0.4055227132852437 1104 108-109 0.4902374216241867 1104 110-111 0.4383799706561504 1104 112-113 0.4863878668849253 1104 114-115 0.41150428946772166 1104 116-117 0.4221000725852626 1104 118-119 0.3096561908021336 1104 120-121 0.3384212012764216 1104 122-123 0.2246733912940755 1104 124-125 -0.01876969788279581 1105 1 0.09389394762538927 1105 2 0.1446647407538748 1105 3 0.15095688220102232 1105 4 0.052142999463427486 1105 5 0.061950551204787985 1105 6 0.09016960218724535 1105 7 0.09280269611014802 1105 8 0.17144334009648787 1105 9 0.23514964345931588 1105 10-11 0.22830931517188446 1105 12-13 0.2538782454701689 1105 14-15 0.2799588589436084 1105 16-17 0.22860158716830625 1105 18-19 0.25755190696016683 1105 20-21 0.19879307282124614 1105 22-23 0.20514857246138263 1105 24-25 0.21130112682008217 1105 26-27 0.25975653710630553 1105 28-29 0.16142488050143555 1105 30-31 0.2535854762135443 1105 32-33 0.22167600659374642 1105 34-35 0.25125016499201536 1105 36-37 0.1596129998728273 1105 38-39 0.19853992097858963 1105 40-41 0.2787818237566384 1105 42-43 0.20957312159616492 1105 44-45 0.2636692603958579 1105 46-47 0.2659276714049099 1105 48-49 0.2507134271355582 1105 50-51 0.22039570507875084 1105 52-53 0.1856004415300916 1105 54-55 0.11883811852069215 1105 56-57 0.10411788590408122 1105 58-59 -0.005185009804982599 1105 60-61 0.014986035008845988 1105 62-63 0.07683053479615154 1105 64-65 -0.03123395920693639 1105 66-67 -0.04481660094987916 1105 68-69 0.030630784651087595 1105 70-71 0.10440783207642568 1105 72-73 0.15674223772890628 1105 74-75 0.18175205270703287 1105 76-77 0.11584759671816869 1105 78-79 0.17317443799184673 1105 80-81 0.20028397829403843 1105 82-83 0.17655079845572885 1105 84-85 0.19511434875437317 1105 86-87 0.13428340939591976 1105 88-89 0.19185275070196894 1105 90-91 0.15352240197029943 1105 92-93 0.21975785595697062 1105 94-95 0.2532449166109103 1105 96-97 0.24208802436810117 1105 98-99 0.18710080308306232 1105 100-101 0.16619455249524862 1105 102-103 0.21240870976788528 1105 104-105 0.15205118184327304 1105 106-107 0.14992540965001666 1105 108-109 0.20853396393088985 1105 110-111 0.1707295032647167 1105 112-113 0.12773062457091555 1105 114-115 0.17469170858613126 1105 116-117 0.12564534331859178 1105 118-119 -0.005834940069163963 1105 120-121 0.08244127776638166 1105 122-123 0.03345570779383422 1105 124-125 -0.13649099246438468 1106 1 0.13376603901701145 1106 2 0.13668988905233448 1106 3 0.11190585691132071 1106 4 0.0925261239138564 1106 5 0.13380832771119344 1106 6 0.005481821308684687 1106 7 0.051336826292057935 1106 8 0.05656790321943106 1106 9 0.11382188936630655 1106 10-11 0.07307330539602219 1106 12-13 0.03682897446092426 1106 14-15 0.12868928108156297 1106 16-17 0.11696605654181269 1106 18-19 0.18632469350844616 1106 20-21 0.050198664305781904 1106 22-23 -0.019097894191304476 1106 24-25 0.15095114299218437 1106 26-27 0.10841646024952922 1106 28-29 0.0918330947381989 1106 30-31 0.13187393940859238 1106 32-33 0.14128344977061147 1106 34-35 0.16109163503352875 1106 36-37 0.21233182306379206 1106 38-39 0.0869104853660474 1106 40-41 0.07088272630630144 1106 42-43 0.17226059745313194 1106 44-45 0.20343751983865843 1106 46-47 0.12875850199007033 1106 48-49 0.11973719823175344 1106 50-51 0.08014063009924399 1106 52-53 0.08472477764762942 1106 54-55 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0.9737278295279985 2204 1 0.18270955840384318 2204 2 0.19044837260278058 2204 3 0.14778728995909773 2204 4 0.16346381038837876 2204 5 0.1674797920622595 2204 6 0.1672303409497573 2204 7 0.24059813189731472 2204 8 0.20928349626735354 2204 9 0.2466420986469302 2204 10-11 0.27185583260705215 2204 12-13 0.24008405736768879 2204 14-15 0.28885386943152014 2204 16-17 0.31876191990758684 2204 18-19 0.3188656646114367 2204 20-21 0.32305905045754457 2204 22-23 0.28626757214070153 2204 24-25 0.36710443774065027 2204 26-27 0.41209169640514887 2204 28-29 0.37373455163488956 2204 30-31 0.3512862404204071 2204 32-33 0.37006067549292965 2204 34-35 0.5025552396690998 2204 36-37 0.49288609562036356 2204 38-39 0.4744751587834344 2204 40-41 0.45050954285935774 2204 42-43 0.4163231921288997 2204 44-45 0.46918763658330676 2204 46-47 0.49790655714537735 2204 48-49 0.4563967447852164 2204 50-51 0.5152904492070078 2204 52-53 0.49610372984513873 2204 54-55 0.4804515745897575 2204 56-57 0.5482149967780359 2204 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0.5124619514747337 2205 58-59 0.5476947703279862 2205 60-61 0.5646335584621056 2205 62-63 0.662689401449768 2205 64-65 0.6626024173242016 2205 66-67 0.6405782347417812 2205 68-69 0.5235487527398277 2205 70-71 0.5161124986081091 2205 72-73 0.41633113218676243 2205 74-75 0.37725372394873347 2205 76-77 0.353408297477948 2205 78-79 0.4383630432324992 2205 80-81 0.47491556935375456 2205 82-83 0.48078676826826694 2205 84-85 0.36472657199818315 2205 86-87 0.44659289835333027 2205 88-89 0.4050776884383964 2205 90-91 0.44170012339460385 2205 92-93 0.4590079159800524 2205 94-95 0.47810644862119034 2205 96-97 0.5427103005096683 2205 98-99 0.470236032592517 2205 100-101 0.593696629791701 2205 102-103 0.5831241129625511 2205 104-105 0.5734773744769761 2205 106-107 0.5618400186591188 2205 108-109 0.6515756570132396 2205 110-111 0.5809085601334019 2205 112-113 0.6132896800897711 2205 114-115 0.6892550503614174 2205 116-117 0.7282850864529955 2205 118-119 0.7428203493195333 2205 120-121 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-0.938894661366426 2216 58-59 -0.7401527070637854 2216 60-61 -0.9467801507719429 2216 62-63 -0.8185760518246212 2216 64-65 -0.7077728716518621 2216 66-67 -0.6968335607593019 2216 68-69 -0.838999617683136 2216 70-71 -0.9360657915022443 2216 72-73 -0.8805402750885492 2216 74-75 -0.919710969852666 2216 76-77 -0.8790710077648498 2216 78-79 -0.7485029962552119 2216 80-81 -0.7770983455217291 2216 82-83 -0.7595798563984673 2216 84-85 -0.7923576611600538 2216 86-87 -0.8234319462287978 2216 88-89 -0.8023885619948814 2216 90-91 -0.6772321748538843 2216 92-93 -0.6778364561041599 2216 94-95 -0.7744233599710348 2216 96-97 -0.8130774819920319 2216 98-99 -0.7991377953587531 2216 100-101 -0.8934239950569705 2216 102-103 -1.0183481566064714 2216 104-105 -0.8700078976557961 2216 106-107 -0.7731621732758853 2216 108-109 -0.6804726605009463 2216 110-111 -0.7069757810170785 2216 112-113 -0.516424140451619 2216 114-115 -0.6097904567552774 2216 116-117 -0.6511406530963235 2216 118-119 -0.6518791195506175 2216 120-121 -0.5758945057873106 2216 122-123 -0.5066752866100188 2216 124-125 -0.44618672865250986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 228.0 3 16.0 4 31.0 5 114.0 6 271.0 7 593.0 8 961.0 9 1611.0 10 2810.0 11 3669.0 12 4761.0 13 6745.0 14 9872.0 15 8316.0 16 4284.0 17 3953.0 18 3847.0 19 3725.0 20 3673.0 21 3849.0 22 4165.0 23 4804.0 24 6057.0 25 8563.0 26 11547.0 27 15194.0 28 19686.0 29 25237.0 30 31887.0 31 40269.0 32 51393.0 33 66804.0 34 93062.0 35 141946.0 36 287787.0 37 595681.0 38 290437.0 39 939.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.298024506695405 18.739631290539982 13.224629541686692 24.73771466107792 2 15.9820944776144 20.66156959313436 38.38833241319159 24.968003516059646 3 18.64591903397057 24.914330160502665 30.10216700487324 26.33758380065352 4 13.07321466442497 16.883340620552687 36.68045078795784 33.36299392706451 5 13.510721390614966 38.105835510720254 34.19302428376628 14.190418814898504 6 32.33861938017514 36.3520874549126 17.28967790693244 14.019615257979822 7 28.45245046728228 32.77343987646031 21.083678694463856 17.690430961793556 8 26.796195332351218 34.46221742598734 19.926346965266404 18.815240276395038 9 27.104248553122122 15.29531432743135 19.56041294369358 38.04002417575295 10-11 25.22812597545922 25.909902677242897 27.830146572609415 21.031824774688467 12-13 25.850088725923037 23.51913563154606 27.986760193246823 22.64401544928408 14-15 22.76958494689806 24.850479336042397 25.72432022752044 26.655615489539098 16-17 22.795426620733494 28.34766233773618 26.137360578626062 22.719550462904262 18-19 22.56649042777778 26.62758480702894 28.321024660746296 22.484900104446986 20-21 23.253714854614 26.024834779266463 28.27821047900438 22.44323988711516 22-23 23.1742388361979 26.027085713051097 28.106160666413842 22.69251478433716 24-25 22.94940289796402 26.198290696454627 28.149465554801562 22.702840850779786 26-27 22.852270165452616 26.694674693815006 27.829977743113627 22.623077397618747 28-29 22.584515140445166 26.45057020979001 28.23225725750626 22.732657392258567 30-31 22.764187426032013 26.017741229809566 28.383011107955525 22.8350602362029 32-33 22.666171627670025 26.25694798224228 28.163208216586007 22.91367217350169 34-35 23.176502394979256 26.297750689610176 27.65852079008019 22.86722612533038 36-37 23.345635372560746 25.796841800627362 27.729281601467378 23.12824122534451 38-39 22.86777762173589 25.975174935907532 27.989091345341986 23.167956097014592 40-41 23.098863504234604 25.8037444631474 28.06192462005699 23.03546741256101 42-43 23.275444951548995 25.838063392554073 27.81240707373889 23.074084582158044 44-45 22.999931486735008 26.0451897575796 27.65013431158525 23.30474444410014 46-47 23.21602900180636 26.30116665633758 27.136742539034003 23.346061802822057 48-49 23.31385106197284 26.242872539094602 27.054572250001918 23.388704148930636 50-51 23.209120831573124 26.305743674475647 27.06146338357061 23.42367211038062 52-53 23.466997424929794 26.18281804448179 27.049352764149383 23.30083176643903 54-55 23.007049745079993 26.16641469376337 27.114084877816357 23.71245068334028 56-57 23.060215734218716 26.482721447681623 27.1160565720246 23.34100624607506 58-59 23.36411029672222 25.876813683097915 27.06297479563994 23.696101224539923 60-61 23.100416110767284 26.089238233293237 27.462798924144572 23.347546731794903 62-63 22.96181970869696 26.14077202071655 27.39632485343592 23.50108341715057 64-65 22.74590385305327 26.074789044949732 27.361784002269747 23.817523099727257 66-67 22.791361318908997 26.333291069356324 27.12102147673368 23.754326135000998 68-69 22.694972554932775 26.17148987995128 27.370498741745724 23.763038823370213 70-71 22.676743311368377 26.110559752420205 27.311757282649147 23.900939653562272 72-73 22.79105257068163 26.127788822337244 26.94085034294992 24.14030826403121 74-75 22.691554659988576 26.44200987442194 26.86880982995946 23.997625635630023 76-77 22.8201092059781 26.26612155597055 26.90576712977954 24.00800210827181 78-79 22.829484184270182 26.332921496463186 26.92568798836926 23.911906330897374 80-81 22.769848679370156 26.724318306646307 26.81577364335091 23.690059370632632 82-83 22.85003812286536 26.37226679524013 26.870337340451115 23.90735774144339 84-85 22.91127350838959 26.157033228014537 26.684442175203703 24.247251088392172 86-87 22.790849602595426 25.91342783406973 27.048158759417714 24.24756380391713 88-89 22.79175932048622 26.057674977129125 26.872469491757673 24.278096210626984 90-91 22.70815701621544 26.372786008989724 26.835150258431028 24.083906716363813 92-93 22.6665025383972 25.80210110718353 27.02592752846138 24.50546882595789 94-95 22.585025929802757 25.732905690114833 26.90195003715629 24.78011834292612 96-97 22.638756142727914 25.874821681079062 26.89063542088951 24.595786755303514 98-99 22.601493533593136 25.83887599486463 26.76082084078641 24.79880963075583 100-101 22.28100958217226 25.779983590963546 27.074995437196204 24.864011389667994 102-103 22.430402317051467 25.484552705927438 26.9706621665955 25.114382810425596 104-105 22.33314920786723 25.50568879460553 27.074744799545364 25.08641719798188 106-107 22.372256309822884 25.749586859796146 26.88827034337047 24.989886487010494 108-109 22.38338035977001 25.63967562419599 26.674522569135966 25.302421446898034 110-111 22.084865225692806 25.92101178244608 26.923162212475223 25.070960779385892 112-113 21.977476522171248 25.89460804520388 27.01395905246059 25.113956380164282 114-115 21.97975081689824 25.81787902685203 26.755542314106258 25.446827842143477 116-117 21.776516679559094 25.81473170817687 27.02659137603271 25.38216023623133 118-119 21.693502396822357 26.105776310604977 26.55187922130423 25.648842071268437 120-121 21.661766959284527 26.227663119602614 26.792543802417416 25.318026118695446 122-123 21.624312376512062 26.252818012537137 26.645477676223507 25.477391934727294 124-125 21.645606217696123 26.25457950694982 26.591686938693243 25.50812733666082 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 51.0 1 50.0 2 81.0 3 121.5 4 97.0 5 87.0 6 206.5 7 482.5 8 861.0 9 981.0 10 742.0 11 556.0 12 551.0 13 628.0 14 743.5 15 827.5 16 926.5 17 1041.5 18 1152.0 19 1277.0 20 1417.5 21 1683.5 22 2058.5 23 2581.5 24 3334.5 25 4326.0 26 5847.0 27 7813.0 28 9703.5 29 11929.5 30 14715.0 31 18147.0 32 22146.0 33 25278.5 34 28726.5 35 32088.5 36 36383.0 37 41387.0 38 44939.5 39 47326.5 40 49177.5 41 54196.0 42 59256.5 43 62597.5 44 66772.5 45 71431.5 46 71613.0 47 70473.0 48 71490.5 49 70556.0 50 68651.0 51 65403.0 52 63867.5 53 64097.5 54 63704.0 55 59977.5 56 52963.0 57 47710.5 58 44707.0 59 40296.0 60 34041.0 61 28258.5 62 23170.5 63 18057.5 64 14526.5 65 12075.0 66 9051.5 67 7779.0 68 5930.5 69 3451.0 70 2528.5 71 2011.0 72 1604.5 73 1173.5 74 952.0 75 925.5 76 583.5 77 239.5 78 113.5 79 60.0 80 25.0 81 6.0 82 5.0 83 3.5 84 1.5 85 1.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027973825141987062 2 0.0 3 0.0 4 0.0 5 0.05378707029333285 6 0.014669200989090778 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.990007886116966E-4 22-23 0.0 24-25 2.842868408738523E-5 26-27 3.127155249612375E-4 28-29 2.5585815678646703E-4 30-31 1.4214342043692614E-4 32-33 1.7057210452431134E-4 34-35 1.990007886116966E-4 36-37 0.0 38-39 0.0 40-41 1.990007886116966E-4 42-43 0.0 44-45 1.990007886116966E-4 46-47 0.0 48-49 4.8328762948554886E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.4214342043692614E-4 58-59 2.2742947269908183E-4 60-61 2.842868408738523E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.685736817477046E-5 70-71 2.842868408738523E-5 72-73 1.4214342043692614E-4 74-75 2.842868408738523E-5 76-77 2.842868408738523E-5 78-79 0.0 80-81 2.842868408738523E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.842868408738523E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.842868408738523E-4 100-101 0.0 102-103 0.0 104-105 0.001279290783932335 106-107 8.528605226215567E-5 108-109 0.001393005520281876 110-111 0.003923158404059161 112-113 0.0 114-115 0.0 116-117 1.4214342043692614E-4 118-119 0.0 120-121 4.8328762948554886E-4 122-123 6.822884180972454E-4 124-125 2.842868408738523E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1758787.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.19969906011195 #Duplication Level Percentage of deduplicated Percentage of total 1 80.24803988296998 52.321480505335046 2 11.604464990974252 15.132152503302521 3 3.7256450838020023 7.287328148060284 4 1.6285947782985761 4.247355577437477 5 0.8638190974841367 2.816037259917161 6 0.4711131980461288 1.8429863241512714 7 0.3227641275441059 1.4730886788292705 8 0.2108260629552348 1.099663668696762 9 0.15253677420036948 0.8950816596117908 >10 0.7100597424164589 8.123515391348452 >50 0.04469610153060612 1.9815913187785195 >100 0.01671348647487108 1.9167126526632832 >500 1.816683258453431E-4 0.08871695410229176 >1k 5.450049775360293E-4 0.7742893577659296 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2705 0.15379918091275407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2493 0.14174541885970274 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.8428684087385225E-5 0.0 22-23 0.0 0.0 0.0 1.7057210452431134E-4 0.0 24-25 0.0 0.0 0.0 5.117163135729341E-4 0.0 26-27 0.0 0.0 0.0 0.00125086209984495 0.0 28-29 0.0 0.0 0.0 0.003951587088146547 0.0 30-31 0.0 0.0 0.0 0.013389910205158442 0.0 32-33 0.0 0.0 0.0 0.0350241387956586 0.0 34-35 0.0 0.0 0.0 0.06893955891190917 0.0 36-37 0.0 0.0 0.0 0.12005433290102781 0.0 38-39 0.0 0.0 0.0 0.1986027870344732 0.0 40-41 0.0 0.0 0.0 0.2855377029736972 0.0 42-43 0.0 0.0 0.0 0.3707384691835907 0.0 44-45 0.0 0.0 0.0 0.47811360898164473 0.0 46-47 0.0 0.0 0.0 0.6116147094560058 0.0 48-49 0.0 0.0 0.0 0.745428525455328 0.0 50-51 0.0 0.0 0.0 0.8924048221871097 0.0 52-53 0.0 0.0 0.0 1.0415416989095325 0.0 54-55 0.0 0.0 0.0 1.1951987364018497 0.0 56-57 0.0 0.0 0.0 1.3847896305806218 0.0 58-59 0.0 0.0 0.0 1.596526469663467 0.0 60-61 0.0 0.0 0.0 1.8259459502486657 0.0 62-63 0.0 0.0 0.0 2.054739999783942 0.0 64-65 0.0 0.0 0.0 2.285012340891762 0.0 66-67 0.0 0.0 0.0 2.553521262097116 0.0 68-69 0.0 0.0 0.0 2.8480708579265137 0.0 70-71 0.0 0.0 0.0 3.181056034642057 0.0 72-73 0.0 0.0 0.0 3.515718503718756 0.0 74-75 0.0 0.0 0.0 3.8581988609194857 0.0 76-77 0.0 0.0 0.0 4.230813623252844 0.0 78-79 0.0 0.0 0.0 4.636746803336617 0.0 80-81 0.0 0.0 0.0 5.078784412211371 0.0 82-83 0.0 0.0 0.0 5.5447874017717895 0.0 84-85 0.0 0.0 0.0 6.009482671864188 0.0 86-87 0.0 0.0 0.0 6.481512542451132 0.0 88-89 0.0 0.0 0.0 6.993797429705814 0.0 90-91 0.0 0.0 0.0 7.551340782027614 0.0 92-93 0.0 0.0 0.0 8.138734252641168 0.0 94-95 0.0 0.0 0.0 8.733888754010577 0.0 96-97 0.0 0.0 0.0 9.330066688007133 0.0 98-99 0.0 0.0 0.0 9.950409003477965 0.0 100-101 0.0 0.0 0.0 10.621524948728869 0.0 102-103 0.0 0.0 0.0 11.324736878314429 0.0 104-105 0.0 0.0 0.0 12.037443988385178 0.0 106-107 0.0 0.0 0.0 12.742759640593205 0.0 108-109 0.0 0.0 0.0 13.473916966636665 0.0 110-111 0.0 0.0 0.0 14.241150292787019 0.0 112-113 0.0 0.0 0.0 15.04372047325799 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2970 0.0 63.368153 1 GTATCAA 4675 0.0 52.869705 1 ATCAACG 5865 0.0 41.219204 3 TCAACGC 5960 0.0 40.462276 4 TATCAAC 6105 0.0 39.89139 2 CAACGCA 6125 0.0 39.080627 5 AACGCAG 6265 0.0 38.090595 6 GTACATG 8300 0.0 35.591312 1 ACATGGG 8475 0.0 32.95146 3 TACATGG 8740 0.0 32.769905 2 ACGCAGA 7555 0.0 31.579525 7 CGCAGAG 7730 0.0 30.710655 8 CATGGGG 4800 0.0 30.516548 4 GTGGTAT 1550 0.0 29.202652 1 TGGTATC 1525 0.0 27.331896 2 GCAGAGT 8700 0.0 26.87627 9 GAGTACT 4435 0.0 26.29409 12-13 ATGGGGA 2875 0.0 25.474682 5 CAGAGTA 8330 0.0 24.855925 10-11 GTACTTT 4880 0.0 23.958012 14-15 >>END_MODULE