FastQCFastQC Report
Thu 26 May 2016
SRR1364346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807413
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.051.222051
GTATCAA30250.048.6139451
TCAACGC32650.043.1930664
ATCAACG33000.042.734963
CAACGCA33150.042.5415885
GTACATG88300.041.129971
AACGCAG35200.039.8927736
TACATGG91750.038.9129372
TATCAAC37950.037.788022
ACATGGG94100.036.1705633
ACGCAGA43650.031.8939557
CATGGGA64700.031.8215034
CGCAGAG44900.031.006048
ATGGGAT30200.028.569985
CATGGGG36400.027.4635564
GAGTACA64600.024.607531
GCAGAGT55450.024.5703169
TAAGGTA7350.022.6683314
AGTACAT68250.022.4067762
TAAGGTG10200.021.5849195
Produced by FastQC (version 0.11.5)