##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364346_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1807413 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.119223995843782 31.0 31.0 34.0 28.0 34.0 2 31.195055031694473 33.0 31.0 34.0 28.0 34.0 3 31.288928983027123 33.0 31.0 34.0 28.0 34.0 4 34.703684769336064 37.0 35.0 37.0 32.0 37.0 5 34.79297094797924 37.0 35.0 37.0 32.0 37.0 6 34.917702262847506 37.0 35.0 37.0 32.0 37.0 7 34.890944681708056 37.0 35.0 37.0 32.0 37.0 8 34.84627531173008 37.0 35.0 37.0 32.0 37.0 9 36.431860897315666 39.0 37.0 39.0 32.0 39.0 10-11 36.34170967011967 39.0 37.0 39.0 32.0 39.0 12-13 36.34851967978541 39.0 37.0 39.0 32.0 39.0 14-15 37.68487418204915 40.0 38.0 41.0 32.0 41.0 16-17 37.69741005514511 40.0 38.0 41.0 32.0 41.0 18-19 37.69847400677101 40.0 38.0 41.0 32.0 41.0 20-21 37.73032090617916 40.0 38.0 41.0 32.0 41.0 22-23 37.70402282156873 40.0 38.0 41.0 32.0 41.0 24-25 37.71642922785219 40.0 38.0 41.0 32.0 41.0 26-27 37.68720347812038 40.0 38.0 41.0 32.0 41.0 28-29 37.69437588420577 40.0 38.0 41.0 32.0 41.0 30-31 37.60385866428979 40.0 38.0 41.0 31.0 41.0 32-33 37.56989852346973 40.0 38.0 41.0 31.0 41.0 34-35 37.56305476390842 40.0 38.0 41.0 31.0 41.0 36-37 37.507434659372265 40.0 38.0 41.0 31.0 41.0 38-39 37.4228601874613 40.0 38.0 41.0 31.0 41.0 40-41 37.355494012713194 40.0 37.5 41.0 31.0 41.0 42-43 37.23238103300132 40.0 37.0 41.0 30.0 41.0 44-45 37.11784937919557 40.0 37.0 41.0 30.0 41.0 46-47 37.010759300724295 40.0 37.0 41.0 30.0 41.0 48-49 36.872037270950244 40.0 36.5 41.0 30.0 41.0 50-51 36.75369796499195 40.0 36.0 41.0 30.0 41.0 52-53 36.594511049771135 40.0 36.0 41.0 30.0 41.0 54-55 36.41103416872624 39.5 35.5 41.0 30.0 41.0 56-57 36.21965123632507 39.0 35.0 41.0 30.0 41.0 58-59 35.99964451954257 39.0 35.0 41.0 29.0 41.0 60-61 35.746203275067735 39.0 35.0 41.0 29.0 41.0 62-63 35.47887477848173 38.0 35.0 40.0 29.0 41.0 64-65 35.13528286008787 37.5 34.0 40.0 28.0 41.0 66-67 34.819117987975076 37.0 34.0 40.0 28.0 41.0 68-69 34.4870428064864 36.5 34.0 39.0 28.0 41.0 70-71 34.12536149734454 36.0 34.0 39.0 27.5 41.0 72-73 33.7565246570651 35.5 34.0 39.0 27.0 40.0 74-75 33.39830603188092 35.0 33.0 37.5 26.0 39.5 76-77 33.15831550398276 35.0 33.0 37.0 26.5 39.0 78-79 32.99222645847961 35.0 33.0 37.0 27.0 39.0 80-81 32.724697398989605 35.0 33.0 36.0 26.5 38.5 82-83 32.44300112923831 35.0 33.0 36.0 26.0 37.0 84-85 32.207802809872454 35.0 33.0 35.5 26.0 37.0 86-87 32.00568215454907 35.0 33.0 35.0 26.0 36.5 88-89 31.75285062130238 35.0 33.0 35.0 25.0 36.0 90-91 31.636048595423404 35.0 33.0 35.0 25.0 36.0 92-93 31.501202824147 35.0 33.0 35.0 25.0 36.0 94-95 31.404007274485686 35.0 33.0 35.0 25.0 35.0 96-97 31.311227705012634 35.0 33.0 35.0 24.5 35.0 98-99 31.184852327608574 35.0 32.5 35.0 24.0 35.0 100-101 31.099428022261655 35.0 32.0 35.0 23.5 35.0 102-103 30.102331896473025 34.0 30.5 35.0 20.0 35.0 104-105 30.802511379524212 34.5 32.0 35.0 23.0 35.0 106-107 31.138040945815924 35.0 33.0 35.0 24.0 35.0 108-109 31.180196778489474 35.0 33.0 35.0 24.0 35.0 110-111 31.116632723124155 35.0 33.0 35.0 23.0 35.0 112-113 31.031584092844305 35.0 33.0 35.0 23.0 35.0 114-115 30.937447611586283 35.0 33.0 35.0 20.0 35.0 116-117 30.81191155535564 35.0 33.0 35.0 19.0 35.0 118-119 30.64449160208541 35.0 33.0 35.0 17.5 35.0 120-121 30.473325963684005 35.0 32.5 35.0 6.0 35.0 122-123 30.295912445025017 35.0 32.0 35.0 2.0 35.0 124-125 29.489867285451638 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13006631835725813 1101 2 0.19658449443709003 1101 3 0.18570264482504228 1101 4 0.3648354931693234 1101 5 0.21236890963658084 1101 6 0.27132196589304414 1101 7 0.3486165094415625 1101 8 0.3008389663470652 1101 9 0.3624386432563469 1101 10-11 0.5143154333128734 1101 12-13 0.4972007919127819 1101 14-15 0.6056966482222563 1101 16-17 0.5213718891562777 1101 18-19 0.35441643504626086 1101 20-21 0.5182560514116545 1101 22-23 0.6210025313717793 1101 24-25 0.611046073673208 1101 26-27 0.6888423630088099 1101 28-29 0.5925352829077681 1101 30-31 0.5927261485715434 1101 32-33 0.5328208766210878 1101 34-35 0.4964926851635809 1101 36-37 0.421516069632915 1101 38-39 0.5444911135067159 1101 40-41 0.5015222668570516 1101 42-43 0.5631784026828299 1101 44-45 0.499142743693497 1101 46-47 0.5111720847476846 1101 48-49 0.4409816461965974 1101 50-51 0.4450044263260935 1101 52-53 0.349609977741018 1101 54-55 0.47384887453397084 1101 56-57 0.3433607947645356 1101 58-59 0.4371519294374622 1101 60-61 0.3870074673589343 1101 62-63 0.3290139350559187 1101 64-65 0.48680291185659286 1101 66-67 0.40354755750499294 1101 68-69 0.3463787561224194 1101 70-71 0.3902308675677659 1101 72-73 0.4029629158058441 1101 74-75 0.3914766313600566 1101 76-77 0.38531096172071955 1101 78-79 0.3605586671047618 1101 80-81 0.40355565022330353 1101 82-83 0.34248090160209443 1101 84-85 0.3851387472373844 1101 86-87 0.37914837278351143 1101 88-89 0.42852052550116326 1101 90-91 0.3491664229739868 1101 92-93 0.28040097423447463 1101 94-95 0.34287385977165385 1101 96-97 0.3215290262155115 1101 98-99 0.2524437877901917 1101 100-101 0.22422130655004224 1101 102-103 -0.013071276517063524 1101 104-105 0.18702925759438216 1101 106-107 0.19722587159527905 1101 108-109 0.21754527512425526 1101 110-111 0.2760690106304118 1101 112-113 0.23200905196639 1101 114-115 0.16508888851543801 1101 116-117 0.1266428621781941 1101 118-119 0.2383928568106235 1101 120-121 0.14488143988924307 1101 122-123 0.02545631559850392 1101 124-125 -0.06750386395114205 1102 1 0.1322446106731654 1102 2 0.11132944440484138 1102 3 0.21227035651726212 1102 4 0.234525418068948 1102 5 0.21782027468820786 1102 6 0.3412622253811364 1102 7 0.32856720531624006 1102 8 0.3662276090620509 1102 9 0.4092792959292382 1102 10-11 0.42206913112227795 1102 12-13 0.3572262241040036 1102 14-15 0.3752835094703215 1102 16-17 0.5652389517815308 1102 18-19 0.4095212496530394 1102 20-21 0.4623910448585775 1102 22-23 0.3515305223846923 1102 24-25 0.3828909737127333 1102 26-27 0.40725842464243556 1102 28-29 0.42087145937631476 1102 30-31 0.5166215732041906 1102 32-33 0.5354737471474778 1102 34-35 0.4324853034137348 1102 36-37 0.3147030335112291 1102 38-39 0.3714889360811995 1102 40-41 0.29561383314717204 1102 42-43 0.21798451473009806 1102 44-45 0.2209189924192856 1102 46-47 0.1560792321122335 1102 48-49 0.20279495969732864 1102 50-51 0.17530791367414622 1102 52-53 0.21955691166239433 1102 54-55 0.23153574132961552 1102 56-57 0.21701637320385458 1102 58-59 0.16467989271247063 1102 60-61 0.17934764502021494 1102 62-63 0.14876826390264597 1102 64-65 0.2376484845403013 1102 66-67 0.277029340636183 1102 68-69 0.13600928723394645 1102 70-71 0.08415435949256533 1102 72-73 0.0746072361231569 1102 74-75 0.0199694403083015 1102 76-77 0.144394258648056 1102 78-79 0.2213507923136575 1102 80-81 0.17222152635969223 1102 82-83 0.25877440677174235 1102 84-85 0.21031496961216334 1102 86-87 0.17820372377202176 1102 88-89 0.2540725098015102 1102 90-91 0.23587701954696172 1102 92-93 0.32117092645593814 1102 94-95 0.19016976080280656 1102 96-97 0.16130188744971363 1102 98-99 0.20713305391458192 1102 100-101 0.23112284392340143 1102 102-103 -0.06982779665825234 1102 104-105 0.12966510398186415 1102 106-107 0.12681666815855408 1102 108-109 0.18834072888169828 1102 110-111 0.10486026209532184 1102 112-113 0.00236303963689366 1102 114-115 -0.06347577894176126 1102 116-117 -0.11197592886523466 1102 118-119 -0.1125878514767713 1102 120-121 -0.2628952283723507 1102 122-123 -0.1485591621522282 1102 124-125 -0.19834139264253636 1103 1 -0.038300956704578226 1103 2 0.0979860085104498 1103 3 0.05531744480445511 1103 4 0.14201122969951285 1103 5 0.017674261564764038 1103 6 0.06492388322478604 1103 7 0.06762712624058054 1103 8 0.09766873329039782 1103 9 0.14216601114868155 1103 10-11 0.23573831208781826 1103 12-13 0.18982854670431237 1103 14-15 0.1549621090238773 1103 16-17 0.1710911234541186 1103 18-19 0.1633630727436426 1103 20-21 0.21641214914934892 1103 22-23 0.2410043167949567 1103 24-25 0.28831228889518457 1103 26-27 0.2904893655822889 1103 28-29 0.16931056425566737 1103 30-31 0.3711270275402683 1103 32-33 0.32465526812672607 1103 34-35 0.35288248760836893 1103 36-37 0.2791401428875915 1103 38-39 0.14413289321424116 1103 40-41 0.10229366455014599 1103 42-43 0.08214679896742183 1103 44-45 0.09361254632229787 1103 46-47 0.09185308712623907 1103 48-49 0.12151114636148463 1103 50-51 0.11756758962446412 1103 52-53 0.10362646113586038 1103 54-55 0.04055168092595807 1103 56-57 0.12651295508346294 1103 58-59 0.134668394288056 1103 60-61 -0.03265840837389078 1103 62-63 0.023062553193000213 1103 64-65 0.07215354338175928 1103 66-67 0.0025456792168014886 1103 68-69 0.0931766384552688 1103 70-71 0.01698585086787574 1103 72-73 0.19932232005525918 1103 74-75 0.06814497419695442 1103 76-77 0.09690538775029722 1103 78-79 0.14428692109601116 1103 80-81 0.19520150851590756 1103 82-83 0.21292473020491798 1103 84-85 0.09276822563793274 1103 86-87 0.10119844973496583 1103 88-89 0.21218919350934584 1103 90-91 0.07562507202677438 1103 92-93 0.0945777374548733 1103 94-95 -0.005801893865250207 1103 96-97 -0.06320688496989035 1103 98-99 -0.049306674450409815 1103 100-101 0.013024719382144667 1103 102-103 -0.24594278613693277 1103 104-105 -0.05316471526545996 1103 106-107 -0.026277844586260812 1103 108-109 -0.01622275556547592 1103 110-111 -0.012469032540025182 1103 112-113 -0.07333921049484005 1103 114-115 -0.21486835620570588 1103 116-117 -0.19329950976656818 1103 118-119 -0.19607923725501664 1103 120-121 -0.35922841165037767 1103 122-123 -0.3749280679000435 1103 124-125 -0.4107662777455374 1104 1 0.14054497346401362 1104 2 0.134456212119904 1104 3 0.19369139838640237 1104 4 0.32759855982849473 1104 5 0.22659164522885789 1104 6 0.2445824279374449 1104 7 0.3632569260890648 1104 8 0.3663172154144547 1104 9 0.4405432969218026 1104 10-11 0.5104719725626836 1104 12-13 0.34166329517103833 1104 14-15 0.5062622922943874 1104 16-17 0.47108263579736587 1104 18-19 0.4862162457126544 1104 20-21 0.4798674427751166 1104 22-23 0.3570533754509526 1104 24-25 0.4785599712933646 1104 26-27 0.42932542492044945 1104 28-29 0.3892941233501972 1104 30-31 0.29607492275857084 1104 32-33 0.4259082977486486 1104 34-35 0.35492109701583985 1104 36-37 0.2715381888403954 1104 38-39 0.15494915430713974 1104 40-41 0.2627732485920973 1104 42-43 0.21586354385675577 1104 44-45 0.18012613128846056 1104 46-47 0.12402916513723028 1104 48-49 0.11705905604024736 1104 50-51 0.06429339744505569 1104 52-53 -0.08215184126991204 1104 54-55 -0.06125933353764168 1104 56-57 -0.13046499901403052 1104 58-59 -0.007556440595593017 1104 60-61 -0.14907055012404413 1104 62-63 -0.00694445698275814 1104 64-65 0.12288615390205138 1104 66-67 0.10489424527435176 1104 68-69 -0.05368104240255178 1104 70-71 -0.15980496611623352 1104 72-73 -0.06095324586711115 1104 74-75 -0.1683054248800886 1104 76-77 -0.1271632491005974 1104 78-79 -0.026948410189802985 1104 80-81 -0.01796048941562134 1104 82-83 -0.06926942337137376 1104 84-85 -0.1303455498289665 1104 86-87 -0.20043165189686363 1104 88-89 -0.14230722124157325 1104 90-91 -0.19754230142069318 1104 92-93 -0.17908396340866872 1104 94-95 -0.12878175297014494 1104 96-97 -0.20264493922173088 1104 98-99 -0.22171691748031463 1104 100-101 -0.3048529577662542 1104 102-103 -0.5386710648087067 1104 104-105 -0.26037828042709066 1104 106-107 -0.28362752661349333 1104 108-109 -0.3012327678568596 1104 110-111 -0.34094684989194235 1104 112-113 -0.279422659878545 1104 114-115 -0.3403355702738402 1104 116-117 -0.24314641758747157 1104 118-119 -0.1897983712625404 1104 120-121 -0.2976567491070341 1104 122-123 -0.3714308389627945 1104 124-125 -0.565095454222778 1105 1 0.023946886166790193 1105 2 0.052709392828031554 1105 3 -0.06734991677488722 1105 4 0.1733396833233556 1105 5 0.1681584185725029 1105 6 0.30175140444090687 1105 7 0.28219594634265377 1105 8 0.2717919921727159 1105 9 0.3487014587287618 1105 10-11 0.23049861108783176 1105 12-13 0.3153656265027891 1105 14-15 0.41507252437116193 1105 16-17 0.41461270393290306 1105 18-19 0.2959750024090795 1105 20-21 0.4507855626669084 1105 22-23 0.3500675370884565 1105 24-25 0.3164051366197569 1105 26-27 0.330676911222497 1105 28-29 0.3110592399497989 1105 30-31 0.3264941624698068 1105 32-33 0.3888578314575355 1105 34-35 0.3415150384639034 1105 36-37 0.2807367056883052 1105 38-39 0.2941098633216228 1105 40-41 0.2947701530980851 1105 42-43 0.3283415044180984 1105 44-45 0.2319125878694237 1105 46-47 0.3569971392090281 1105 48-49 0.2814941765270902 1105 50-51 0.28413193216385935 1105 52-53 0.25914982606572323 1105 54-55 0.30603181484057274 1105 56-57 0.2466800377552758 1105 58-59 0.12376711418257713 1105 60-61 0.18305577805389817 1105 62-63 0.2611746507600756 1105 64-65 0.12651948036690186 1105 66-67 0.0764401573212794 1105 68-69 0.03343179044489375 1105 70-71 0.0470619713832221 1105 72-73 -2.389768556980698E-4 1105 74-75 -0.008488717080474828 1105 76-77 0.057076347967978336 1105 78-79 0.24352904472649328 1105 80-81 0.23010826565492692 1105 82-83 0.15006635492171227 1105 84-85 0.21531469246799162 1105 86-87 0.11263491195347086 1105 88-89 0.150664560298857 1105 90-91 0.07974169144745602 1105 92-93 0.1164121230692885 1105 94-95 0.05793895737584975 1105 96-97 0.006206269910908446 1105 98-99 0.02249498740669509 1105 100-101 -0.06535059446716929 1105 102-103 -0.15693945888501304 1105 104-105 -0.0030660286587718133 1105 106-107 -0.12336865451004542 1105 108-109 -0.11680816192573573 1105 110-111 -0.1081006790526935 1105 112-113 -0.09980972868065763 1105 114-115 -0.1640806530959118 1105 116-117 -0.22757080343370006 1105 118-119 -0.16800198885293582 1105 120-121 -0.28480585469578656 1105 122-123 -0.4494419923149202 1105 124-125 -0.5579160099316312 1106 1 0.09351134205209988 1106 2 0.08588241296771315 1106 3 0.06713680882454653 1106 4 0.04729603555428952 1106 5 0.01925332557946291 1106 6 0.07106183081607043 1106 7 0.09103779830979164 1106 8 0.059174646116566976 1106 9 0.17391707575923476 1106 10-11 0.22313297943492216 1106 12-13 0.16887827995429205 1106 14-15 0.27389183061392686 1106 16-17 0.2851917039627665 1106 18-19 0.1883777524493695 1106 20-21 0.13787222999182802 1106 22-23 0.218263698880655 1106 24-25 0.15753008486278475 1106 26-27 0.28469580766041247 1106 28-29 0.2713071998711385 1106 30-31 0.2966179836956684 1106 32-33 0.29476756892360356 1106 34-35 0.2428273852612719 1106 36-37 0.2802980998750044 1106 38-39 0.2490049624784163 1106 40-41 0.2475777549352145 1106 42-43 0.1972718147231305 1106 44-45 0.0799452581844804 1106 46-47 0.20708367658664173 1106 48-49 0.13308701312089966 1106 50-51 0.11954498301737715 1106 52-53 0.11562308557211765 1106 54-55 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-0.7916945678870064 2216 92-93 -0.8911024958404248 2216 94-95 -0.7948135586300502 2216 96-97 -0.7283311400732231 2216 98-99 -0.8603996121960691 2216 100-101 -0.7666836614977868 2216 102-103 -0.7623875686905031 2216 104-105 -0.8847559413336725 2216 106-107 -0.7290006796312731 2216 108-109 -0.6314243531310915 2216 110-111 -0.5868008443822994 2216 112-113 -0.683313218355881 2216 114-115 -0.5560418061866876 2216 116-117 -0.6007985588346543 2216 118-119 -0.5244227138620907 2216 120-121 -0.4800233907298761 2216 122-123 -0.5713031154466393 2216 124-125 -0.6099865135766507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24341.0 3 4641.0 4 3115.0 5 2509.0 6 2790.0 7 3406.0 8 3639.0 9 3922.0 10 4455.0 11 5146.0 12 5706.0 13 5997.0 14 5839.0 15 5525.0 16 5123.0 17 4517.0 18 4432.0 19 4288.0 20 4196.0 21 4431.0 22 5285.0 23 7150.0 24 10745.0 25 15478.0 26 18263.0 27 18243.0 28 21651.0 29 26773.0 30 31611.0 31 38793.0 32 51069.0 33 68520.0 34 97485.0 35 150624.0 36 300184.0 37 582909.0 38 253859.0 39 753.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72704910365121 18.66050557758273 12.351385774548643 24.26105954421742 2 15.591065556399686 20.145491527749574 39.44411767472512 24.819325241125618 3 18.552033445171343 25.379002487584213 29.29716893441099 26.771795132833454 4 12.18374548919232 16.26828806756501 37.321103110340914 34.22686333290176 5 13.115564235280056 39.22876380517991 34.38637403771179 13.269297921828246 6 32.371207735671334 36.729164060917526 17.601729368831293 13.297898834579849 7 29.05515017517302 32.61842160592601 21.097503756869152 17.22892446203183 8 27.040169612777614 35.27249751163404 19.83328639695122 17.854046478637123 9 27.146036904680887 15.809502310761292 19.247012166007437 37.79744861855038 10-11 24.834238126116237 27.03987801342472 27.820275620611127 20.305608239847917 12-13 25.341804466433143 24.45220823584024 27.788532986461718 22.4174543112649 14-15 22.475793326839675 25.830161427616233 24.944084182594434 26.749961062949662 16-17 22.724051142767195 29.254469514681496 25.733327948098133 22.28815139445317 18-19 22.97926538055518 27.953643109595376 26.931739968529588 22.135351541319853 20-21 23.495798551797115 27.28735139322097 26.71642472318854 22.500425331793377 22-23 23.35830272328461 27.342450231352768 26.61953853380495 22.67970851155768 24-25 22.948407475214573 27.519360544601597 26.72468882319647 22.80754315698736 26-27 22.82657979468416 27.647842036991598 26.557718902263765 22.96785926606048 28-29 22.79227413171953 27.714210705548858 26.718364996070342 22.775150166661273 30-31 22.846640050281948 27.30495826066942 26.713863167585846 23.13453852146278 32-33 22.621811702432844 27.494362791407926 26.670698217145468 23.21312728901376 34-35 23.33555753262159 27.443686815035147 26.381106035658007 22.839649616685257 36-37 23.3742123045233 27.077802278787555 26.35226070918666 23.19572470750248 38-39 22.810111048467725 27.317032282123193 26.61929314283596 23.253563526573124 40-41 23.11407374456471 27.110060246729972 26.78509085698193 22.990775151723387 42-43 22.96598142630692 27.011748953604496 26.660552895188932 23.361716724899647 44-45 22.75909040987659 27.257604976912788 26.85603043733614 23.127274175874486 46-47 23.226697406760994 27.499631378908457 26.24593237345231 23.02773884087824 48-49 22.99200570096597 27.397833256704473 26.27404472580423 23.33611631652533 50-51 22.996293597534155 27.252266084176668 26.605015013170757 23.14642530511842 52-53 23.194638247111588 27.163557032943565 26.51178990303166 23.130014816913192 54-55 22.739783479112702 27.207999316127523 26.690142968631775 23.362074236128 56-57 22.79407716832765 27.51823393940232 26.633156515183998 23.05453237708603 58-59 22.954682202774425 26.93251779833214 26.59316339332274 23.5196366055707 60-61 22.607757720415435 27.17608918227542 26.916379029650056 23.299774067659087 62-63 22.69576473016155 27.18864139784902 26.99045676246974 23.125137109519695 64-65 22.679209977254505 27.204600443401567 26.631841145607122 23.484348433736805 66-67 22.554281822291248 27.380762023113103 26.56062141019037 23.504334744405277 68-69 22.67467466359773 26.93483865970255 27.03728089589235 23.353205780807365 70-71 22.69401521757413 27.05462701894008 26.71410724007737 23.537250523408417 72-73 22.81657878574946 27.143943962774642 26.527495753498176 23.511981497977725 74-75 22.649477833875096 27.445162182723564 26.529414205685043 23.3759457777163 76-77 22.837636450099495 27.117091122758957 26.689790230838202 23.355482196303345 78-79 22.950450057915372 27.236519633269957 26.62784801301996 23.18518229579471 80-81 22.924365944660085 27.449030883424115 26.59506632064351 23.03153685127229 82-83 22.93307078445698 27.345988830976047 26.600778628196874 23.1201617563701 84-85 22.82982821208109 27.276318563153872 26.635288311461252 23.258564913303783 86-87 22.950805159639025 27.16650589017384 26.66633284911345 23.216356101073686 88-89 22.95446019416831 27.1667428393104 26.540172124353596 23.3386248421677 90-91 22.86842185343075 27.374301830524978 26.64317073373151 23.114105582312764 92-93 23.00777530459685 26.951973260875217 26.78551767217793 23.254733762349996 94-95 22.822229912855168 26.84337970674344 26.9070625738114 23.427327806589986 96-97 23.032179820140026 26.761225756692188 26.77079748545706 23.43579693771073 98-99 22.858258112544448 26.95029300351914 26.586843045112 23.604605838824412 100-101 22.694166296889335 26.912283652595946 26.8930294114067 23.500520639108018 102-103 22.838906610888245 26.62107420674957 26.86988609815427 23.670133084207915 104-105 22.712789324764106 26.826050488049674 26.761676177576827 23.699484009609396 106-107 22.788954733330012 26.84646625610721 26.701553154170053 23.663025856392718 108-109 22.81612688425425 26.954023767559608 26.449564134151128 23.780285214035015 110-111 22.695565789462766 27.229229101292717 26.66286007368081 23.412345035563703 112-113 22.770675703713948 27.237540632132234 26.785808147174773 23.205975516979045 114-115 22.7390200894228 27.430923071049683 26.434197997199245 23.39585884232827 116-117 22.712316412939135 27.363622028510804 26.592590953228008 23.331470605322053 118-119 22.575637432970076 27.549697284206886 26.30963501124373 23.565030271579314 120-121 22.574304979930435 27.73852460195638 26.419616049897797 23.267554368215386 122-123 22.67241064502457 27.822432057684708 26.348388960141467 23.15676833714925 124-125 22.723841071877626 27.858178497435915 26.28321088564852 23.13476954503794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 742.0 1 615.0 2 428.5 3 332.0 4 287.0 5 252.5 6 218.0 7 183.5 8 193.5 9 204.0 10 177.0 11 166.0 12 166.0 13 185.0 14 192.0 15 217.0 16 259.5 17 298.5 18 411.5 19 523.0 20 690.5 21 1029.5 22 1489.5 23 2149.5 24 3079.5 25 4377.0 26 6353.5 27 8825.5 28 11468.5 29 14683.0 30 18553.5 31 22518.5 32 27756.0 33 31341.5 34 33961.0 35 37402.0 36 41951.0 37 47028.5 38 50097.0 39 52573.5 40 53823.0 41 56815.5 42 60133.5 43 62566.5 44 65657.0 45 69169.5 46 70068.5 47 68829.0 48 68548.0 49 67656.5 50 66435.0 51 64987.0 52 64569.5 53 65583.5 54 66858.5 55 63748.5 56 57205.5 57 51874.5 58 47715.0 59 41400.5 60 33012.0 61 27056.0 62 22922.0 63 17849.5 64 13304.0 65 10251.5 66 7399.5 67 5823.0 68 4238.0 69 2501.5 70 1741.0 71 1260.0 72 985.0 73 714.5 74 494.5 75 406.5 76 295.5 77 164.0 78 89.5 79 62.5 80 46.0 81 30.5 82 21.5 83 16.5 84 11.5 85 12.5 86 11.0 87 7.0 88 6.0 89 4.5 90 3.5 91 3.0 92 2.0 93 1.5 94 1.5 95 4.0 96 4.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04459412430916453 2 0.008852431624648046 3 0.0020471248131998607 4 0.004813509695902375 5 0.021688457480387714 6 0.028272453501219697 7 0.0031536787662808665 8 1.1065539530810059E-4 9 0.0 10-11 5.5327697654050294E-5 12-13 0.0011065539530810057 14-15 4.702854300594275E-4 16-17 1.1065539530810059E-4 18-19 5.5327697654050294E-5 20-21 2.7663848827025147E-5 22-23 0.0 24-25 0.0 26-27 1.3831924413512572E-4 28-29 2.489746394432263E-4 30-31 2.7663848827025144E-4 32-33 1.3831924413512572E-4 34-35 0.0011618816507350561 36-37 2.489746394432263E-4 38-39 2.2131079061620118E-4 40-41 6.639323718486036E-4 42-43 9.958985577729052E-4 44-45 0.001521511685486383 46-47 8.299154648107544E-5 48-49 0.0 50-51 0.0 52-53 0.0010512262554269556 54-55 0.0032366703127619423 56-57 2.7663848827025147E-5 58-59 5.809408253675281E-4 60-61 7.469239183296789E-4 62-63 4.1495773240537716E-4 64-65 0.0012172093483891063 66-67 0.0022131079061620115 68-69 0.0029323679756646653 70-71 0.0016045032319674584 72-73 0.0023790909991241627 74-75 0.002102452510853911 76-77 0.0011895454995620813 78-79 0.001908805569064735 80-81 0.0010788901042539808 82-83 1.93646941789176E-4 84-85 5.532769765405029E-4 86-87 0.002130116359680936 88-89 0.006362685230215784 90-91 4.4262158123240235E-4 92-93 0.0013278647436972071 94-95 0.0010788901042539808 96-97 3.87293883578352E-4 98-99 6.362685230215784E-4 100-101 0.0010512262554269556 102-103 0.001300200894870182 104-105 8.299154648107543E-4 106-107 0.006805306811448186 108-109 0.0018534778714106848 110-111 0.0012448731972161315 112-113 0.002102452510853911 114-115 0.0024344186967782126 116-117 0.001549175534313408 118-119 0.04705620685476977 120-121 0.011010211833156008 122-123 0.003928266533437571 124-125 0.0023514271502971373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1807413.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.30266643043412 #Duplication Level Percentage of deduplicated Percentage of total 1 83.74869992193503 58.04008214672387 2 9.938516134338066 13.77531336943037 3 2.9607041696119842 6.155540803974444 4 1.2081389426344795 3.3490900057205883 5 0.6195025475665922 2.1466589203405837 6 0.3674204487014213 1.5277930077645019 7 0.23783657973022293 1.1537896404999293 8 0.1560651442430978 0.8652584506317593 9 0.10536016956327265 0.6571566617667715 >10 0.5922576923183765 7.696945657770236 >50 0.04552909065180532 2.2109875960461745 >100 0.019799928554209895 2.3041027320058336 >500 1.6923015140076788E-4 0.1172810073249928 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.5327697654050294E-5 2 0.0 0.0 0.0 0.0 1.1065539530810059E-4 3 0.0 0.0 0.0 0.0 1.1065539530810059E-4 4 0.0 0.0 0.0 0.0 1.6598309296215086E-4 5 0.0 0.0 0.0 0.0 1.6598309296215086E-4 6 0.0 0.0 0.0 0.0 2.2131079061620118E-4 7 0.0 0.0 0.0 0.0 2.2131079061620118E-4 8 0.0 0.0 0.0 0.0 2.2131079061620118E-4 9 0.0 0.0 0.0 0.0 2.2131079061620118E-4 10-11 0.0 0.0 0.0 0.0 2.2131079061620118E-4 12-13 0.0 0.0 0.0 0.0 2.2131079061620118E-4 14-15 0.0 0.0 0.0 0.0 2.2131079061620118E-4 16-17 0.0 0.0 0.0 0.0 2.2131079061620118E-4 18-19 0.0 0.0 0.0 0.0 2.2131079061620118E-4 20-21 0.0 0.0 0.0 8.299154648107543E-5 2.2131079061620118E-4 22-23 0.0 0.0 0.0 2.7663848827025144E-4 2.7663848827025144E-4 24-25 0.0 0.0 0.0 4.702854300594275E-4 3.319661859243017E-4 26-27 0.0 0.0 0.0 0.0011065539530810057 3.319661859243017E-4 28-29 0.0 0.0 0.0 0.00381761113812947 3.319661859243017E-4 30-31 0.0 0.0 0.0 0.013195655890490994 3.596300347513269E-4 32-33 0.0 0.0 0.0 0.03413718945254903 3.8729388357835207E-4 34-35 0.0 0.0 0.0 0.06384816309277404 3.8729388357835207E-4 36-37 0.0 0.0 0.0 0.1176266852125109 3.8729388357835207E-4 38-39 0.0 0.0 0.0 0.19801782990384598 3.8729388357835207E-4 40-41 0.0 0.0 0.0 0.29008311880018567 3.8729388357835207E-4 42-43 0.0 0.0 0.0 0.38118017298757945 3.8729388357835207E-4 44-45 0.0 0.0 0.0 0.4883499233434749 3.8729388357835207E-4 46-47 0.0 0.0 0.0 0.620555456887828 3.8729388357835207E-4 48-49 0.0 0.0 0.0 0.761336783568559 4.979492788864526E-4 50-51 0.0 0.0 0.0 0.9188824026384672 4.979492788864526E-4 52-53 0.0 0.0 0.0 1.06494752444516 4.979492788864526E-4 54-55 0.0 0.0 0.0 1.219948069422982 4.979492788864526E-4 56-57 0.0 0.0 0.0 1.4030274209602343 4.979492788864526E-4 58-59 0.0 0.0 0.0 1.60848682619855 4.979492788864526E-4 60-61 0.0 0.0 0.0 1.8395076277530371 4.979492788864526E-4 62-63 0.0 0.0 0.0 2.0721329325394917 5.532769765405029E-4 64-65 0.0 5.5327697654050294E-5 0.0 2.3001383745718327 6.086046741945532E-4 66-67 0.0 5.5327697654050294E-5 0.0 2.56128510749895 6.639323718486034E-4 68-69 0.0 5.5327697654050294E-5 0.0 2.8522811333104277 6.639323718486034E-4 70-71 0.0 5.5327697654050294E-5 0.0 3.1771377100861837 6.639323718486034E-4 72-73 0.0 5.5327697654050294E-5 0.0 3.5053969402676644 7.745877671567041E-4 74-75 0.0 5.5327697654050294E-5 0.0 3.8290363076950316 7.745877671567041E-4 76-77 0.0 5.5327697654050294E-5 0.0 4.1769368705437 7.745877671567041E-4 78-79 0.0 5.5327697654050294E-5 0.0 4.570095490073381 7.745877671567041E-4 80-81 0.0 5.5327697654050294E-5 0.0 5.000296003182449 8.299154648107544E-4 82-83 0.0 5.5327697654050294E-5 0.0 5.455974921061207 9.40570860118855E-4 84-85 0.0 5.5327697654050294E-5 0.0 5.896991999061642 9.40570860118855E-4 86-87 0.0 5.5327697654050294E-5 0.0 6.3376217831784984 9.40570860118855E-4 88-89 0.0 5.5327697654050294E-5 0.0 6.824450194836487 9.40570860118855E-4 90-91 0.0 5.5327697654050294E-5 0.0 7.359275384209365 9.40570860118855E-4 92-93 0.0 5.5327697654050294E-5 0.0 7.923534908734196 9.40570860118855E-4 94-95 0.0 5.5327697654050294E-5 0.0 8.484696082190402 9.958985577729052E-4 96-97 0.0 5.5327697654050294E-5 0.0 9.036755849382516 9.958985577729052E-4 98-99 0.0 5.5327697654050294E-5 0.0 9.617143397773503 9.958985577729052E-4 100-101 0.0 5.5327697654050294E-5 0.0 10.262983612489233 9.958985577729052E-4 102-103 0.0 5.5327697654050294E-5 0.0 10.939475371705305 0.0010512262554269556 104-105 0.0 5.5327697654050294E-5 0.0 11.610655671946589 0.0010512262554269556 106-107 0.0 5.5327697654050294E-5 0.0 12.25303790555894 0.0011895454995620811 108-109 0.0 5.5327697654050294E-5 0.0 12.924135214253742 0.001355528592524232 110-111 0.0 5.5327697654050294E-5 0.0 13.64959198589365 0.0013831924413512573 112-113 0.0 5.5327697654050294E-5 0.0 14.409517913172031 0.0013831924413512573 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1790 0.0 51.22205 1 GTATCAA 3025 0.0 48.613945 1 TCAACGC 3265 0.0 43.193066 4 ATCAACG 3300 0.0 42.73496 3 CAACGCA 3315 0.0 42.541588 5 GTACATG 8830 0.0 41.12997 1 AACGCAG 3520 0.0 39.892773 6 TACATGG 9175 0.0 38.912937 2 TATCAAC 3795 0.0 37.78802 2 ACATGGG 9410 0.0 36.170563 3 ACGCAGA 4365 0.0 31.893955 7 CATGGGA 6470 0.0 31.821503 4 CGCAGAG 4490 0.0 31.00604 8 ATGGGAT 3020 0.0 28.56998 5 CATGGGG 3640 0.0 27.463556 4 GAGTACA 6460 0.0 24.60753 1 GCAGAGT 5545 0.0 24.570316 9 TAAGGTA 735 0.0 22.668331 4 AGTACAT 6825 0.0 22.406776 2 TAAGGTG 1020 0.0 21.584919 5 >>END_MODULE