FastQCFastQC Report
Thu 26 May 2016
SRR1364346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807413
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.19525144502114347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32680.18081091593343634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.10401607158961455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.069.926891
GTATCAA49800.051.9154131
ATCAACG63700.039.917933
GTACATG87850.039.6010781
TCAACGC64600.039.453984
CAACGCA65200.038.9082375
TATCAAC68000.038.269442
AACGCAG67600.037.5847976
TACATGG92150.037.0287322
ACATGGG90450.036.276273
CATGGGA62750.031.8863744
ACGCAGA80800.031.515737
GTGGTAT14950.031.4790671
TGGTATC14600.030.9984742
CGCAGAG82050.030.8905758
CATGGGG35500.028.0135234
ATGGGAG26500.027.4153355
GCAGAGT94150.026.7309889
GTGTAAG9000.025.1522621
ATGGGAT27750.024.8928495
Produced by FastQC (version 0.11.5)