FastQCFastQC Report
Thu 26 May 2016
SRR1364345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843343
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.054.9511
GTATCAA23900.046.07991
ATCAACG26500.040.6514553
GTACATG75700.039.7130431
TCAACGC27450.038.8109324
CAACGCA28500.037.381065
AACGCAG28950.037.000576
TACATGG80800.036.9036872
ACATGGG81350.035.410433
TATCAAC30800.034.976092
CATGGGG41400.030.0461884
ACGCAGA35900.029.8310347
CGCAGAG36600.029.0971478
CATGGGA50250.029.0183984
ATGGGGA24950.027.4328355
TAGTGCG1200.001288823124.7894699
GAGTACA55050.024.4393041
ATGGGAT24450.022.3950675
GTGTAAG10550.021.4421751
GCAGAGT48300.021.432889
Produced by FastQC (version 0.11.5)