FastQCFastQC Report
Thu 26 May 2016
SRR1364345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843343
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.157431362475676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.14430304072546454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.069.121091
GTATCAA46950.055.810381
ATCAACG57000.045.6492583
TCAACGC58400.044.5549284
CAACGCA58900.044.0756075
TATCAAC60350.043.213952
AACGCAG61100.042.6568686
ACGCAGA71200.036.3501667
GTACATG72800.035.5022771
CGCAGAG73600.035.0848
TACATGG76100.033.8007432
ACATGGG77250.032.4497073
GCAGAGT85450.029.8008679
GAGTACT48650.026.23261612-13
CAGAGTA81450.026.04147510-11
CATGGGA47900.025.9799254
CATGGGG40400.025.3497834
AGAGTAC78250.025.01547210-11
GTACTTT51500.024.26102814-15
GAGTACA55400.023.8638821
Produced by FastQC (version 0.11.5)