FastQCFastQC Report
Thu 26 May 2016
SRR1364344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964033
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.054.1331371
GTATCAA23550.044.501811
GTACATG70350.037.4122731
ATCAACG27650.035.9473653
TCAACGC28250.035.183884
TACATGG72950.035.163952
ACATGGG71800.034.8982243
CAACGCA28600.034.7533045
TATCAAC31050.033.3528752
AACGCAG29800.033.151596
CATGGGG46350.028.3784224
ACGCAGA36050.027.3964147
CATGGGA32300.027.0869734
CGCAGAG37850.026.0922168
TATTCCG2757.4578566E-1125.9713335
ATTCCGT3151.6370905E-1124.5609576
CCGTTGC2853.2068783E-922.9623629
GTATAGG6300.022.6843621
GAGTACA53850.022.6685661
GTGTAAG11100.021.4581811
Produced by FastQC (version 0.11.5)