FastQCFastQC Report
Thu 26 May 2016
SRR1364344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964033
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26360.13421363083003188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25410.1293766448934412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.064.650141
GTATCAA48000.053.726131
ATCAACG57900.043.398353
CAACGCA59050.042.6540035
TCAACGC59350.042.5387234
TATCAAC59400.042.5029142
AACGCAG61050.041.5259786
ACGCAGA70250.035.9100467
CGCAGAG71650.035.2083858
GTACATG63750.033.165541
GTGGTAT14500.031.2165221
TACATGG66750.030.7756422
ACATGGG67250.029.484333
TAAGGTG13000.028.8560265
GCAGAGT87900.028.0225669
TGGTATC14700.027.5442452
GTAAGGT11500.026.9243264
AGGTAAG12250.025.761972
CATGGGA29400.025.5189324
GAGTACT50750.025.49879312-13
Produced by FastQC (version 0.11.5)