FastQCFastQC Report
Thu 26 May 2016
SRR1364343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131275
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25050.044.686841
GGTATCA17200.044.310951
GTACATG75500.039.8266681
ATCAACG26450.038.7015533
CAACGCA26950.037.7626955
TCAACGC27200.037.415614
TACATGG80450.037.3586352
ACATGGG78800.036.8569373
AACGCAG29300.034.7266126
TATCAAC31000.033.78912
CATGGGA40850.029.8667134
CATGGGG46350.029.6611444
ACGCAGA35850.028.3772017
CGCAGAG37150.027.2240498
GTGTAAG12650.024.0054131
GAGTACA55200.023.083641
ATGGGAT22750.021.451525
GTGTACG1954.0186933E-521.3743531
TAAGGTG11200.021.255325
ATGGGGA26400.020.7400385
Produced by FastQC (version 0.11.5)