##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364343_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2131275 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.211851121980974 31.0 31.0 34.0 28.0 34.0 2 31.34692472815568 33.0 31.0 34.0 28.0 34.0 3 31.423602772988 33.0 31.0 34.0 28.0 34.0 4 34.90729774430798 37.0 35.0 37.0 32.0 37.0 5 34.97042146133183 37.0 35.0 37.0 32.0 37.0 6 35.038342776037815 37.0 35.0 37.0 32.0 37.0 7 34.95978182074111 37.0 35.0 37.0 32.0 37.0 8 34.882168185710434 37.0 35.0 37.0 32.0 37.0 9 36.51712378740425 39.0 37.0 39.0 32.0 39.0 10-11 36.42478000258062 39.0 37.0 39.0 32.0 39.0 12-13 36.44035495184807 39.0 37.0 39.0 32.0 39.0 14-15 37.78238378435444 40.0 38.0 41.0 32.0 41.0 16-17 37.777881080573835 40.0 38.0 41.0 32.0 41.0 18-19 37.77303797022908 40.0 38.0 41.0 32.0 41.0 20-21 37.78643347292114 40.0 38.0 41.0 32.0 41.0 22-23 37.74847309708977 40.0 38.0 41.0 32.0 41.0 24-25 37.74182238331515 40.0 38.0 41.0 32.0 41.0 26-27 37.68962827415514 40.0 38.0 41.0 32.0 41.0 28-29 37.6905061524205 40.0 38.0 41.0 32.0 41.0 30-31 37.5975153370635 40.0 38.0 41.0 31.5 41.0 32-33 37.562980375596766 40.0 38.0 41.0 31.0 41.0 34-35 37.54752272700614 40.0 38.0 41.0 31.0 41.0 36-37 37.48998275680051 40.0 38.0 41.0 31.0 41.0 38-39 37.398184889326814 40.0 38.0 41.0 31.0 41.0 40-41 37.337774102356576 40.0 37.5 41.0 31.0 41.0 42-43 37.207522023202074 40.0 37.0 41.0 31.0 41.0 44-45 37.08833913971684 40.0 37.0 41.0 30.0 41.0 46-47 36.97191610655594 40.0 37.0 41.0 30.0 41.0 48-49 36.82940634127459 40.0 36.5 41.0 30.0 41.0 50-51 36.704413320664855 40.0 36.0 41.0 30.0 41.0 52-53 36.54655194660474 40.0 36.0 41.0 30.0 41.0 54-55 36.357270647851635 39.0 35.0 41.0 30.0 41.0 56-57 36.16056679687041 39.0 35.0 41.0 29.0 41.0 58-59 35.94037817738209 39.0 35.0 41.0 29.0 41.0 60-61 35.68807427478856 38.5 35.0 40.5 29.0 41.0 62-63 35.417698560720694 38.0 35.0 40.0 28.5 41.0 64-65 35.07491478105828 37.5 34.0 40.0 28.0 41.0 66-67 34.764147517331175 37.0 34.0 40.0 28.0 41.0 68-69 34.43470833186707 36.5 34.0 39.0 28.0 41.0 70-71 34.075393837022446 36.0 34.0 39.0 27.0 41.0 72-73 33.70996469249627 35.5 33.5 38.5 26.5 40.0 74-75 33.35304055084398 35.0 33.0 37.5 26.0 39.5 76-77 33.123129816659045 35.0 33.0 37.0 26.5 39.0 78-79 32.96187000738995 35.0 33.0 37.0 26.5 39.0 80-81 32.70261392828236 35.0 33.0 36.0 26.0 38.5 82-83 32.41961595758407 35.0 33.0 36.0 26.0 37.0 84-85 32.18456581154473 35.0 33.0 35.5 26.0 37.0 86-87 31.98592063436206 35.0 33.0 35.0 26.0 36.5 88-89 31.735011436815988 35.0 33.0 35.0 25.0 36.0 90-91 31.619345931426025 35.0 33.0 35.0 25.0 36.0 92-93 31.479218073688287 35.0 33.0 35.0 25.0 36.0 94-95 31.3858141253475 35.0 33.0 35.0 25.0 35.0 96-97 31.289914018603888 35.0 33.0 35.0 24.0 35.0 98-99 31.160929255961808 35.0 32.0 35.0 24.0 35.0 100-101 31.076274061301334 35.0 32.0 35.0 23.5 35.0 102-103 30.06259304876189 33.5 30.5 34.5 20.0 35.0 104-105 30.77189640004223 34.5 32.0 35.0 21.5 35.0 106-107 31.104666220924095 35.0 32.5 35.0 24.0 35.0 108-109 31.140647499736076 35.0 33.0 35.0 23.5 35.0 110-111 31.070411842676332 35.0 33.0 35.0 23.0 35.0 112-113 30.98320676590304 35.0 33.0 35.0 21.5 35.0 114-115 30.885358529518715 35.0 33.0 35.0 20.0 35.0 116-117 30.755141640567267 35.0 33.0 35.0 19.0 35.0 118-119 30.58030615476651 35.0 32.5 35.0 16.5 35.0 120-121 30.398534914546456 35.0 32.0 35.0 4.5 35.0 122-123 30.211265322400912 35.0 32.0 35.0 2.0 35.0 124-125 29.406892118567523 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23456094000883 1101 2 0.23216625667582136 1101 3 0.2586531950683053 1101 4 0.30504478586979644 1101 5 0.27107591314064194 1101 6 0.20103789094160618 1101 7 0.24424104396526758 1101 8 0.04287217491015127 1101 9 0.21340141870916085 1101 10-11 0.30647233045291955 1101 12-13 0.35660057496846775 1101 14-15 0.40668216890365727 1101 16-17 0.38505077746566485 1101 18-19 0.16274631290646369 1101 20-21 0.3354338222612512 1101 22-23 0.45956986645016684 1101 24-25 0.4789190291155805 1101 26-27 0.5236141727612988 1101 28-29 0.4419240037984622 1101 30-31 0.5266943248082541 1101 32-33 0.3834532846448795 1101 34-35 0.40986365183311335 1101 36-37 0.3272410997096813 1101 38-39 0.5184005883403202 1101 40-41 0.4341118068749026 1101 42-43 0.46130297212564386 1101 44-45 0.4976123017309604 1101 46-47 0.4523081827198894 1101 48-49 0.41147827752973143 1101 50-51 0.3631011933084878 1101 52-53 0.3219861436821674 1101 54-55 0.4039029340317839 1101 56-57 0.2931549948432277 1101 58-59 0.3489181691055947 1101 60-61 0.3113131512563143 1101 62-63 0.43493647205864505 1101 64-65 0.4016269528672751 1101 66-67 0.34394087808416884 1101 68-69 0.28546997229524607 1101 70-71 0.2932734568888833 1101 72-73 0.3617934255373427 1101 74-75 0.3409486027063764 1101 76-77 0.3230535517784219 1101 78-79 0.3684795937586003 1101 80-81 0.2914404545901661 1101 82-83 0.24132947211375466 1101 84-85 0.2281801087890969 1101 86-87 0.2403986356507133 1101 88-89 0.27136773221799615 1101 90-91 0.28064717250698124 1101 92-93 0.2752356112542458 1101 94-95 0.14615430640548155 1101 96-97 0.10682780705976569 1101 98-99 0.09907449279376834 1101 100-101 0.10940562884821503 1101 102-103 -0.08502095965783951 1101 104-105 0.0027412463916114405 1101 106-107 0.04764776127202808 1101 108-109 0.1081381997041504 1101 110-111 0.10857815449283947 1101 112-113 0.0986159416116692 1101 114-115 0.057715511848172696 1101 116-117 0.0302052629721139 1101 118-119 0.12113293938384118 1101 120-121 -0.06890296480818847 1101 122-123 -0.11733714882655022 1101 124-125 -0.17026680119910154 1102 1 0.0923324788148534 1102 2 0.1433972670447652 1102 3 0.20691613902090467 1102 4 0.22718012179824143 1102 5 0.25838888752257816 1102 6 0.3027321736703712 1102 7 0.266821911373718 1102 8 0.3123699185458264 1102 9 0.39683580041940303 1102 10-11 0.41380465330247773 1102 12-13 0.3061696840203396 1102 14-15 0.3247253326364756 1102 16-17 0.4280458570986738 1102 18-19 0.36335820101835026 1102 20-21 0.40093943733583615 1102 22-23 0.47560053118773027 1102 24-25 0.4347383534693918 1102 26-27 0.38544415312576064 1102 28-29 0.393160267534725 1102 30-31 0.4822696402973605 1102 32-33 0.44834744221144973 1102 34-35 0.3236969449767457 1102 36-37 0.3052157113049816 1102 38-39 0.374602576007824 1102 40-41 0.3968667243712005 1102 42-43 0.2712975752236986 1102 44-45 0.22505655288210846 1102 46-47 0.1843211410259471 1102 48-49 0.19415321753225356 1102 50-51 0.13851686958623333 1102 52-53 0.20837992231732727 1102 54-55 0.21601395519452637 1102 56-57 0.19926110138036535 1102 58-59 0.08384057428661862 1102 60-61 0.040872385125894084 1102 62-63 0.04707074583084392 1102 64-65 0.1123386549582861 1102 66-67 0.13911409567117516 1102 68-69 0.08907871986950511 1102 70-71 0.022160231034277444 1102 72-73 0.10807731406261212 1102 74-75 0.13760740367552415 1102 76-77 0.19670360861813663 1102 78-79 0.28804278918386217 1102 80-81 0.32601435769854703 1102 82-83 0.2866562717508927 1102 84-85 0.19017213974663605 1102 86-87 0.10056504969643498 1102 88-89 0.2211607552868884 1102 90-91 0.1765235662282052 1102 92-93 0.23939357972255948 1102 94-95 0.1101063026850646 1102 96-97 0.15820243817577762 1102 98-99 0.06981495922389058 1102 100-101 0.046497272836411696 1102 102-103 -0.20487165493956638 1102 104-105 0.06779903343215565 1102 106-107 0.07891462659062043 1102 108-109 0.015302874110204812 1102 110-111 0.03624387957236053 1102 112-113 0.11046831337783303 1102 114-115 -0.01566010678606844 1102 116-117 -0.059108440565729836 1102 118-119 -0.11144626302294824 1102 120-121 -0.19840205766934815 1102 122-123 -0.12941343460973087 1102 124-125 -0.1656973763710532 1103 1 0.24538145240862264 1103 2 0.3023216224723768 1103 3 0.2339107233896378 1103 4 0.3395971991335216 1103 5 0.2685723101948412 1103 6 0.24170368966172617 1103 7 0.23656173653863988 1103 8 0.316117880562679 1103 9 0.4078425850391483 1103 10-11 0.46891774984716506 1103 12-13 0.4995562716196744 1103 14-15 0.5971035605252979 1103 16-17 0.4895438312638305 1103 18-19 0.42611938960865814 1103 20-21 0.5081240505421221 1103 22-23 0.5461096164453849 1103 24-25 0.5513332962550521 1103 26-27 0.6101954689130338 1103 28-29 0.530688208639738 1103 30-31 0.6101160094617768 1103 32-33 0.6409606225375626 1103 34-35 0.655921710430647 1103 36-37 0.5844971096504636 1103 38-39 0.5596500842644829 1103 40-41 0.627088129352174 1103 42-43 0.527458442260901 1103 44-45 0.4677132157674393 1103 46-47 0.4777085036034663 1103 48-49 0.452448730534293 1103 50-51 0.4814269084440568 1103 52-53 0.514057496208352 1103 54-55 0.3831967503439202 1103 56-57 0.4480704626140337 1103 58-59 0.44925671243213827 1103 60-61 0.26871282675453756 1103 62-63 0.29889016418669456 1103 64-65 0.38507023100417825 1103 66-67 0.3665127997401072 1103 68-69 0.33392930407737254 1103 70-71 0.3937416347417724 1103 72-73 0.4043885436220336 1103 74-75 0.34337437234629675 1103 76-77 0.4010300604245458 1103 78-79 0.4853007069473634 1103 80-81 0.43663233367338705 1103 82-83 0.41101743839048055 1103 84-85 0.3598550773244469 1103 86-87 0.3130136916833308 1103 88-89 0.4023470918770826 1103 90-91 0.3542659626450373 1103 92-93 0.37074002261462624 1103 94-95 0.34544759050609386 1103 96-97 0.3220394221450391 1103 98-99 0.27548683597806445 1103 100-101 0.3080000434881143 1103 102-103 0.11045596813115566 1103 104-105 0.2255880458334829 1103 106-107 0.2237141669657241 1103 108-109 0.2239701462372956 1103 110-111 0.22928109863229906 1103 112-113 0.17245871286441883 1103 114-115 0.15524111742810476 1103 116-117 0.05341263046248912 1103 118-119 1.2860154438243399E-4 1103 120-121 -0.12174299695965374 1103 122-123 -0.1307075434351006 1103 124-125 -0.012293483718519127 1104 1 0.23765631011187338 1104 2 0.303681378382759 1104 3 0.292568448612716 1104 4 0.41396229632183434 1104 5 0.31986578482053574 1104 6 0.3258348757130136 1104 7 0.4156454068657922 1104 8 0.455513561656403 1104 9 0.5820508851847137 1104 10-11 0.5983222644064341 1104 12-13 0.43367253327443933 1104 14-15 0.5197618641444777 1104 16-17 0.5436590064063722 1104 18-19 0.5164051221279351 1104 20-21 0.5177847194459062 1104 22-23 0.5433924270949788 1104 24-25 0.5146480592499856 1104 26-27 0.6169744569783688 1104 28-29 0.5418544442020448 1104 30-31 0.5025302961685227 1104 32-33 0.5494132837250874 1104 34-35 0.482007354251742 1104 36-37 0.499529329382824 1104 38-39 0.38788340731163373 1104 40-41 0.4536648241900636 1104 42-43 0.4377800862306316 1104 44-45 0.4097946419823515 1104 46-47 0.4603714642806125 1104 48-49 0.32525833804891846 1104 50-51 0.329071337827223 1104 52-53 0.22483521277164442 1104 54-55 0.28423578319455345 1104 56-57 0.2345036610959781 1104 58-59 0.2668491831043909 1104 60-61 0.1438144095887779 1104 62-63 0.22276160515232135 1104 64-65 0.3220433331621706 1104 66-67 0.3166391623233693 1104 68-69 0.24778302156481402 1104 70-71 0.15235799979695486 1104 72-73 0.26009477252794255 1104 74-75 0.18918950289740621 1104 76-77 0.19921112182985468 1104 78-79 0.3174227541787431 1104 80-81 0.32143901527926033 1104 82-83 0.3129211370558025 1104 84-85 0.24251700803647935 1104 86-87 0.21318139423909344 1104 88-89 0.3002746203000797 1104 90-91 0.2273420483898363 1104 92-93 0.1808905913977199 1104 94-95 0.1066944056923802 1104 96-97 0.052670221252562044 1104 98-99 0.12480132350781048 1104 100-101 0.12098957985032754 1104 102-103 -0.08045210566443117 1104 104-105 0.020036291499955894 1104 106-107 0.10866262024085316 1104 108-109 0.1217025871496098 1104 110-111 0.08377681559303696 1104 112-113 0.11953971093696936 1104 114-115 0.17591322419550082 1104 116-117 0.0955144742212255 1104 118-119 0.017568132494226063 1104 120-121 -0.02620323061228902 1104 122-123 -0.08366509837060931 1104 124-125 -0.3088372035163047 1105 1 0.09088646112308396 1105 2 0.09409869043924246 1105 3 -0.07014132809241502 1105 4 0.11132270200686634 1105 5 0.1016838029034588 1105 6 0.17393572862817308 1105 7 0.16762970538209743 1105 8 0.23689161400001524 1105 9 0.2537219745226764 1105 10-11 0.20943712778391443 1105 12-13 0.3439096665029169 1105 14-15 0.3294964438306067 1105 16-17 0.2716343034858113 1105 18-19 0.20094848631990203 1105 20-21 0.34777203178255434 1105 22-23 0.2634680027426697 1105 24-25 0.32430744351808016 1105 26-27 0.3453454930841673 1105 28-29 0.2453376480571805 1105 30-31 0.2458446580430973 1105 32-33 0.29323755358407766 1105 34-35 0.24250534503818244 1105 36-37 0.14683215028664165 1105 38-39 0.17204444057572488 1105 40-41 0.17696519761561547 1105 42-43 0.23430633808675339 1105 44-45 0.09899902142753803 1105 46-47 0.13919172171610228 1105 48-49 0.08018281243145253 1105 50-51 0.1398197798862313 1105 52-53 0.07038672588556949 1105 54-55 0.15296404506472783 1105 56-57 0.18663459818198191 1105 58-59 0.1807825488573016 1105 60-61 0.10530194806202076 1105 62-63 0.043250380372548136 1105 64-65 0.03154742363281571 1105 66-67 0.07947826714268302 1105 68-69 -0.04003081799427832 1105 70-71 -0.11109822776449363 1105 72-73 -0.06742039663423327 1105 74-75 -0.14950992155496579 1105 76-77 -0.018470808055795374 1105 78-79 0.14926203510442093 1105 80-81 0.03562678469198488 1105 82-83 0.08001926432466888 1105 84-85 0.0960421372145035 1105 86-87 0.06940515338963138 1105 88-89 0.06252086703310766 1105 90-91 0.06616384675605147 1105 92-93 0.1977198562737037 1105 94-95 0.09337015784668523 1105 96-97 0.10102812942910333 1105 98-99 0.1642273368198488 1105 100-101 0.12510401496913914 1105 102-103 -0.0055381576398261245 1105 104-105 0.041109919971553666 1105 106-107 -0.02683722166365854 1105 108-109 -0.01097429969003727 1105 110-111 -0.01342144215464458 1105 112-113 0.005968213752673535 1105 114-115 -0.13326236786210188 1105 116-117 -0.10349077494927883 1105 118-119 -0.014766997924546388 1105 120-121 -0.1427635330349517 1105 122-123 -0.09981070031418682 1105 124-125 -0.22129542828406557 1106 1 0.10585271185650313 1106 2 0.1674085930917215 1106 3 0.12943867888652605 1106 4 0.12674480707568847 1106 5 0.09071633037216031 1106 6 0.1360366990102051 1106 7 0.020228490737146387 1106 8 0.2027101750816911 1106 9 0.30127504395153437 1106 10-11 0.3177769615845989 1106 12-13 0.18686383651647276 1106 14-15 0.32747308274583986 1106 16-17 0.266439874265501 1106 18-19 0.3081632801270118 1106 20-21 0.20928187044530944 1106 22-23 0.30006718054229253 1106 24-25 0.3113061704897504 1106 26-27 0.35554073909182193 1106 28-29 0.299260305544891 1106 30-31 0.3574911273468757 1106 32-33 0.2770576728446983 1106 34-35 0.25241805028620945 1106 36-37 0.23550084490580048 1106 38-39 0.27320461692446685 1106 40-41 0.23375487535675887 1106 42-43 0.21432655961495328 1106 44-45 0.060575846318961624 1106 46-47 0.1983172179506667 1106 48-49 0.1304688813257684 1106 50-51 0.0865918266200012 1106 52-53 0.1422919959521991 1106 54-55 0.10096919904122359 1106 56-57 0.12907612539066804 1106 58-59 0.07503362252185042 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110-111 -0.803607761418462 2216 112-113 -0.737941992205446 2216 114-115 -0.7390903521476879 2216 116-117 -0.8039525961990499 2216 118-119 -0.6704695950340209 2216 120-121 -0.5403954602495915 2216 122-123 -0.6198890142043894 2216 124-125 -0.5815034269115102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28914.0 3 5482.0 4 3642.0 5 3048.0 6 3249.0 7 3831.0 8 4240.0 9 4804.0 10 5248.0 11 5889.0 12 6463.0 13 6671.0 14 6647.0 15 6138.0 16 5628.0 17 5049.0 18 4858.0 19 4949.0 20 5071.0 21 5381.0 22 6466.0 23 8594.0 24 12091.0 25 17167.0 26 20444.0 27 22189.0 28 26383.0 29 32634.0 30 38683.0 31 48434.0 32 61865.0 33 83758.0 34 117531.0 35 181545.0 36 358409.0 37 680030.0 38 289209.0 39 641.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54272979690702 18.172937677938137 12.131408413349263 25.152924111805586 2 15.809639021203933 19.728849444902483 39.13297892558047 25.328532608313115 3 18.859899165499492 24.029438606616413 29.028605748364196 28.082056479519895 4 12.352351328409874 15.633385996432924 37.11570757105178 34.89855510410542 5 13.403589295938575 38.155698852063566 34.77604445320493 13.664667398792929 6 33.51706374274989 36.05346329631504 17.128182936914325 13.301290024020748 7 29.428863686485013 31.847277811926674 21.019524303323493 17.704334198264817 8 26.79465596132263 35.14319148377119 19.743092388019924 18.319060166886253 9 26.687733868224417 15.520240231786136 19.317685423044892 38.474340476944555 10-11 25.098045839729156 26.206279954215166 27.955234276595657 20.740439929460017 12-13 25.57404132506338 23.47257406337786 27.84299253630148 23.11039207525728 14-15 22.611582493825836 25.176075461481812 24.808874752229844 27.40346729246251 16-17 23.087368268191078 28.414000750256363 25.74085469636318 22.757776285189383 18-19 23.161798257333814 27.355791100583243 26.7439740868668 22.73843655521614 20-21 23.42225274125881 26.905379856043883 26.762226076462696 22.910141326234605 22-23 23.373831010505686 26.704537590066412 26.630638146329815 23.290993253098087 24-25 23.17021501214062 26.98703827521085 26.57700202930171 23.265744683346824 26-27 22.95791694496272 27.244954718387852 26.4178670639878 23.379261272661626 28-29 23.119083021741588 27.185924201681537 26.553129813758147 23.141862962818728 30-31 23.13279626663651 26.882276916305948 26.490538381250417 23.494388435807124 32-33 22.77397983362033 27.121106194897877 26.571035110520956 23.533878860960836 34-35 23.53234845127527 27.075169612624684 26.238807349759192 23.153674586340856 36-37 23.55839746555347 26.720027551595898 26.152714292015013 23.56886069083562 38-39 23.10060344330068 26.955701040084566 26.397371706715578 23.54632380989917 40-41 23.35947906847549 26.62231572981041 26.660086831304874 23.35811837040923 42-43 23.15379212263994 26.58162099465712 26.540588678834503 23.72399820386844 44-45 23.0960579911159 26.917924590716225 26.65995064805513 23.326066770112746 46-47 23.468738291917955 27.168099260840993 26.07715527828784 23.28600716895321 48-49 23.232923953971216 27.006322506480863 26.275891191892175 23.484862347655746 50-51 23.176361567606246 26.998651042216515 26.47492698032868 23.350060409848563 52-53 23.39401156696424 26.7902941101949 26.37771778146264 23.43797654137822 54-55 23.027368136557744 26.818188429904076 26.58817727656847 23.56626615696971 56-57 23.009816199050988 27.213993702355353 26.585308404882902 23.190881693710754 58-59 23.06932193600121 26.660368355042724 26.5290609916486 23.74124871730747 60-61 22.850156340322666 26.86597242554597 26.83655304309963 23.447318191031734 62-63 22.951639246959417 26.895704076428277 26.84507658171038 23.30758009490192 64-65 22.86193888036486 26.840158030808496 26.61897120442186 23.67893188440478 66-67 22.665232421602358 27.092146335226243 26.52793434692386 23.714686896247542 68-69 22.940395213073327 26.704461074254894 26.905734594387752 23.44940911828403 70-71 22.83018646596785 26.66742523061908 26.717021236662568 23.7853670667505 72-73 22.94212180930931 26.80541478978979 26.503777214714713 23.748686186186184 74-75 22.918133452764227 27.056001062303093 26.498385782593388 23.527479702339296 76-77 23.16128885440716 26.844071870785175 26.54140790708139 23.453231367726275 78-79 23.13695423378649 26.84849997724288 26.610488639973422 23.40405714899721 80-81 23.040079240235908 27.063158756602085 26.51216509821393 23.38459690494808 82-83 23.06815433228771 27.066801141203662 26.486064454405696 23.37898007210294 84-85 23.025119002653366 27.03554947671677 26.523011089710053 23.41632043091981 86-87 23.12070624353803 26.899810179101685 26.616732814240862 23.362750763119426 88-89 23.198645978453712 27.00833312608833 26.415925237889894 23.377095657568063 90-91 23.074389363282457 27.166735718183478 26.492533286662955 23.266341631871114 92-93 23.206387070831436 26.736674325733855 26.685178555425164 23.37176004800954 94-95 22.988960664964218 26.66582990907886 26.822217556190814 23.52299186976611 96-97 23.307975924146422 26.52970256148464 26.678675189934236 23.4836463244347 98-99 23.03523397626513 26.61939399484575 26.487312038343653 23.858059990545467 100-101 22.931953165249492 26.534929463532464 26.871963619267298 23.661153751950746 102-103 22.936086094399418 26.38949495176991 26.835244425550925 23.839174528279756 104-105 22.858743524060596 26.632248753023163 26.670301458601024 23.838706264315217 106-107 23.017774761054646 26.637013519179327 26.48080190194331 23.864409817822718 108-109 23.00913420053575 26.606672888452206 26.38853502288125 23.995657888130793 110-111 23.02040835478021 26.781983216304162 26.56527787878859 23.63233055012704 112-113 23.01521741527522 26.82957728932796 26.826996595125912 23.328208700270903 114-115 22.907169833985 27.05038914823763 26.436718776760248 23.605722241017123 116-117 22.869057547956633 26.912394177520177 26.67630901982313 23.54223925470006 118-119 22.774591383685767 27.19092890090215 26.31379686213465 23.720682853277427 120-121 22.768275191174723 27.35208082518309 26.42496760277423 23.454676380867955 122-123 22.797192493796757 27.495256069040853 26.421466592590647 23.286084844571743 124-125 22.878821541846428 27.556345828143552 26.242362546960234 23.322470083049787 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 407.0 1 382.5 2 320.0 3 260.5 4 224.0 5 188.0 6 149.5 7 150.0 8 165.0 9 147.5 10 133.5 11 138.5 12 134.0 13 131.0 14 150.0 15 176.5 16 199.0 17 259.0 18 343.5 19 433.5 20 563.5 21 789.0 22 1115.0 23 1684.0 24 2502.0 25 3627.0 26 5405.5 27 7791.5 28 10436.5 29 13624.0 30 17622.0 31 22722.5 32 29633.0 33 34149.5 34 36783.5 35 41531.0 36 49057.0 37 55389.0 38 58524.5 39 62232.5 40 65023.0 41 68788.0 42 72041.5 43 74798.0 44 78677.0 45 81943.5 46 82318.5 47 81352.5 48 81269.5 49 79779.5 50 79047.5 51 77730.5 52 76628.5 53 78436.5 54 81373.0 55 77372.5 56 68591.5 57 63041.0 58 59262.5 59 52214.0 60 42261.0 61 34766.5 62 28786.5 63 22311.0 64 16836.5 65 12886.5 66 9209.0 67 7136.0 68 5511.0 69 3646.0 70 2544.0 71 1768.5 72 1278.5 73 927.0 74 706.0 75 547.5 76 361.5 77 223.0 78 132.0 79 80.0 80 48.0 81 25.0 82 17.5 83 12.0 84 10.5 85 11.0 86 10.0 87 8.5 88 8.5 89 9.0 90 6.5 91 6.5 92 5.0 93 3.5 94 3.0 95 3.0 96 2.5 97 2.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04551266260806325 2 0.009759416311832119 3 0.002815216243797727 4 0.00553659194613553 5 0.023225534011331248 6 0.028949807040386622 7 0.003988223012046779 8 1.4076081218988634E-4 9 0.0 10-11 7.038040609494317E-5 12-13 0.0012199270389790149 14-15 7.272641963144127E-4 16-17 1.6422094755486738E-4 18-19 2.3460135364981057E-5 20-21 2.3460135364981057E-5 22-23 2.3460135364981057E-5 24-25 0.0 26-27 7.038040609494317E-5 28-29 6.568837902194695E-4 30-31 3.049817597447537E-4 32-33 2.3460135364981053E-4 34-35 0.0010087858206941855 36-37 4.6920270729962115E-5 38-39 2.815216243797727E-4 40-41 7.507243316793938E-4 42-43 0.001313767580438939 44-45 0.0024398540779580298 46-47 7.038040609494317E-5 48-49 0.0 50-51 0.0 52-53 0.0015483689340887498 54-55 0.0034721000340171963 56-57 9.384054145992423E-5 58-59 7.507243316793938E-4 60-61 0.001266847309708977 62-63 0.0010322459560591665 64-65 0.002111412182848295 66-67 0.0034017196279222534 68-69 0.004480885854711382 70-71 0.0025806148901479165 72-73 0.0035190203047471584 74-75 0.0026509952962428594 76-77 0.0015718290694537308 78-79 0.0034721000340171963 80-81 0.0016187493401836927 82-83 7.741844670443749E-4 84-85 9.384054145992421E-4 86-87 0.0026040750255128973 88-89 0.006662678443654621 90-91 7.507243316793938E-4 92-93 0.0015014486633587877 94-95 0.0015249087987237687 96-97 5.161229780295833E-4 98-99 0.0010557060914241476 100-101 0.0017360500170085981 102-103 0.0015483689340887498 104-105 9.618655499642234E-4 106-107 0.008985231844787745 108-109 0.002533694619417954 110-111 0.0018768108291984842 112-113 0.0030732777328125183 114-115 0.0038474621998568935 116-117 0.002463314213323011 118-119 0.04865632074697071 120-121 0.014334142708003424 122-123 0.0061465554656250365 124-125 0.003917842605951837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2131275.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.4226915893412 #Duplication Level Percentage of deduplicated Percentage of total 1 84.47680879139953 57.80130634385677 2 9.69402478439715 13.265825361644723 3 2.792747796315221 5.73261963462266 4 1.0928071917137245 2.990912377809689 5 0.5409063506573292 1.8505134204871236 6 0.31481564390806815 1.2924320226372963 7 0.1963690834753919 0.9405270865422835 8 0.13117687723212965 0.7180380011605503 9 0.09909140923208748 0.6102090839736287 >10 0.5649312111097706 7.496860791013166 >50 0.06147312320737581 2.9149131747488464 >100 0.034055743392182314 4.076632516123105 >500 7.919939599354945E-4 0.30921018538010586 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.1730067682490529E-4 14-15 4.6920270729962115E-5 0.0 0.0 2.3460135364981057E-5 1.6422094755486738E-4 16-17 4.6920270729962115E-5 0.0 0.0 9.384054145992423E-5 1.8768108291984846E-4 18-19 1.4076081218988634E-4 0.0 0.0 9.384054145992423E-5 1.8768108291984846E-4 20-21 2.3460135364981055E-4 0.0 0.0 9.384054145992423E-5 1.8768108291984846E-4 22-23 2.3460135364981055E-4 0.0 0.0 1.1730067682490529E-4 2.111412182848295E-4 24-25 2.815216243797727E-4 0.0 0.0 1.4076081218988634E-4 2.3460135364981055E-4 26-27 2.815216243797727E-4 0.0 0.0 7.741844670443749E-4 3.049817597447537E-4 28-29 2.815216243797727E-4 0.0 0.0 0.0028152162437977265 3.2844189510973477E-4 30-31 2.815216243797727E-4 0.0 0.0 0.011683147411760566 3.2844189510973477E-4 32-33 2.815216243797727E-4 0.0 0.0 0.030943918546410015 3.519020304747158E-4 34-35 3.049817597447537E-4 0.0 0.0 0.058579958006357694 3.9882230120467796E-4 36-37 3.2844189510973477E-4 0.0 0.0 0.10700167739967859 4.22282436569659E-4 38-39 4.22282436569659E-4 0.0 0.0 0.18113570515301874 4.692027072996211E-4 40-41 4.22282436569659E-4 0.0 0.0 0.26458340664625635 4.692027072996211E-4 42-43 4.926628426646022E-4 0.0 0.0 0.34308101957748294 4.692027072996211E-4 44-45 5.630432487595453E-4 0.0 0.0 0.4390095130848905 4.692027072996211E-4 46-47 7.507243316793937E-4 0.0 0.0 0.5565447912634456 4.692027072996211E-4 48-49 7.976446024093559E-4 0.0 0.0 0.6832999026404383 5.161229780295833E-4 50-51 7.976446024093559E-4 0.0 0.0 0.8237791932059448 7.038040609494316E-4 52-53 7.976446024093559E-4 0.0 0.0 0.9565635593717376 7.038040609494316E-4 54-55 7.976446024093559E-4 0.0 0.0 1.0969255492604193 7.741844670443749E-4 56-57 8.44564873139318E-4 0.0 0.0 1.2663077265955827 7.976446024093559E-4 58-59 8.44564873139318E-4 0.0 0.0 1.4524404405813423 7.976446024093559E-4 60-61 8.914851438692801E-4 0.0 0.0 1.6586550304395256 7.976446024093559E-4 62-63 8.914851438692801E-4 0.0 0.0 1.8605060351198226 9.384054145992422E-4 64-65 8.914851438692801E-4 0.0 0.0 2.067049066873116 9.853256853292044E-4 66-67 8.914851438692801E-4 0.0 0.0 2.305709023941068 9.853256853292044E-4 68-69 9.853256853292044E-4 0.0 0.0 2.579559184056492 9.853256853292044E-4 70-71 0.0010557060914241476 0.0 0.0 2.867907707827474 9.853256853292044E-4 72-73 0.0011026263621541096 0.0 0.0 3.1643499782993745 0.0010322459560591665 74-75 0.0011260864975190907 0.0 0.0 3.4584931555055074 0.0010791662267891286 76-77 0.0011260864975190907 0.0 0.0 3.7801316113593977 0.0011026263621541096 78-79 0.0011495466328840717 0.0 0.0 4.1367491290424745 0.0011260864975190907 80-81 0.0011730067682490528 0.0 0.0 4.5228794970146975 0.0011260864975190907 82-83 0.0011730067682490528 0.0 0.0 4.925948082720437 0.0011730067682490528 84-85 0.001243387174343996 0.0 0.0 5.324770383925115 0.0012199270389790149 86-87 0.001313767580438939 0.0 0.0 5.733985525096481 0.001243387174343996 88-89 0.001313767580438939 0.0 0.0 6.1752237510410435 0.001266847309708977 90-91 0.0014076081218988633 0.0 0.0 6.666455525448382 0.001266847309708977 92-93 0.0014545283926288256 0.0 0.0 7.177323433156209 0.001313767580438939 94-95 0.0014545283926288256 0.0 0.0 7.684273498258085 0.001313767580438939 96-97 0.0014545283926288256 0.0 0.0 8.199153089113324 0.0013372277158039201 98-99 0.0014779885279938066 0.0 0.0 8.738290459935953 0.0013606878511689012 100-101 0.0015483689340887498 0.0 0.0 9.333450634010159 0.0013606878511689012 102-103 0.0015952892048187119 0.0 0.0 9.953548931977338 0.0014310682572638445 104-105 0.0019237310999284465 0.0 0.0 10.56839215962276 0.0015014486633587877 106-107 0.0019237310999284465 0.0 0.0 11.174766278401426 0.0015014486633587877 108-109 0.0019237310999284465 0.0 0.0 11.810887848822887 0.0015249087987237687 110-111 0.001970651370658409 4.6920270729962115E-5 0.0 12.499958944763112 0.0015952892048187119 112-113 0.001970651370658409 4.6920270729962115E-5 0.0 13.207915449672145 0.0015952892048187119 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2505 0.0 44.68684 1 GGTATCA 1720 0.0 44.31095 1 GTACATG 7550 0.0 39.826668 1 ATCAACG 2645 0.0 38.701553 3 CAACGCA 2695 0.0 37.762695 5 TCAACGC 2720 0.0 37.41561 4 TACATGG 8045 0.0 37.358635 2 ACATGGG 7880 0.0 36.856937 3 AACGCAG 2930 0.0 34.726612 6 TATCAAC 3100 0.0 33.7891 2 CATGGGA 4085 0.0 29.866713 4 CATGGGG 4635 0.0 29.661144 4 ACGCAGA 3585 0.0 28.377201 7 CGCAGAG 3715 0.0 27.224049 8 GTGTAAG 1265 0.0 24.005413 1 GAGTACA 5520 0.0 23.08364 1 ATGGGAT 2275 0.0 21.45152 5 GTGTACG 195 4.0186933E-5 21.374353 1 TAAGGTG 1120 0.0 21.25532 5 ATGGGGA 2640 0.0 20.740038 5 >>END_MODULE