FastQCFastQC Report
Thu 26 May 2016
SRR1364343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131275
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25380.11908364711264384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.10580521049606458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.062.478021
GTATCAA44100.053.8938261
ATCAACG54850.042.7731553
CAACGCA55250.042.4634865
TCAACGC55400.042.3485154
AACGCAG56900.041.3057376
TATCAAC59050.040.1342242
GTACATG75150.037.254061
ACATGGG77850.035.0314873
TACATGG81350.034.402662
ACGCAGA71050.033.160887
CGCAGAG72150.032.7377748
CATGGGA38700.031.6962574
GCAGAGT84800.027.7138089
CATGGGG46850.025.6739984
GTACTAG4000.025.3159121
AGAGTAC78950.024.03994810-11
CAGAGTA81450.023.99602110-11
TAAGGTG11300.023.7129615
GAGTACT48700.022.72369612-13
Produced by FastQC (version 0.11.5)