FastQCFastQC Report
Thu 26 May 2016
SRR1364342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100997
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30400.052.0974541
GGTATCA18600.049.9367261
ATCAACG34100.045.0349433
CAACGCA36100.042.538925
TCAACGC36050.042.4338234
AACGCAG38650.039.568926
TATCAAC39850.039.2836382
GTACATG102950.038.4016761
TACATGG106750.036.131952
ACATGGG109450.034.5880173
ACGCAGA48950.031.360717
CATGGGA69950.031.0591414
CGCAGAG49250.031.045918
CATGGGG44850.027.870274
ATGGGAT31000.026.1126635
ATGGGAG27850.026.0740575
AGTACAT75400.024.6301782
GAGTACA78600.024.5431841
GCAGAGT61500.024.0880589
TAAGGTG9400.024.0618885
Produced by FastQC (version 0.11.5)