##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364342_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2100997 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.308875738518427 31.0 31.0 34.0 28.0 34.0 2 31.37305860027406 33.0 31.0 34.0 28.0 34.0 3 31.514788931159824 34.0 31.0 34.0 28.0 34.0 4 34.940509672312714 37.0 35.0 37.0 32.0 37.0 5 35.038456980186076 37.0 35.0 37.0 32.0 37.0 6 35.08150606592965 37.0 35.0 37.0 32.0 37.0 7 35.02847410062937 37.0 35.0 37.0 32.0 37.0 8 34.93934831891716 37.0 35.0 37.0 32.0 37.0 9 36.53940533946503 39.0 37.0 39.0 32.0 39.0 10-11 36.46322055671665 39.0 37.0 39.0 32.0 39.0 12-13 36.47490619929491 39.0 37.0 39.0 32.0 39.0 14-15 37.8218876561937 40.0 38.0 41.0 32.0 41.0 16-17 37.827283665802476 40.0 38.0 41.0 32.0 41.0 18-19 37.84004284632486 40.0 38.0 41.0 32.0 41.0 20-21 37.87345436476111 40.0 38.0 41.0 32.0 41.0 22-23 37.83530533361066 40.0 38.0 41.0 32.0 41.0 24-25 37.85571564357303 40.0 38.0 41.0 32.0 41.0 26-27 37.821068759260484 40.0 38.0 41.0 32.0 41.0 28-29 37.8293205559075 40.0 38.0 41.0 32.0 41.0 30-31 37.74095488951198 40.0 38.0 41.0 32.0 41.0 32-33 37.70095150064469 40.0 38.0 41.0 31.5 41.0 34-35 37.69082297594903 40.0 38.0 41.0 32.0 41.0 36-37 37.63145378122863 40.0 38.0 41.0 31.0 41.0 38-39 37.54563904660502 40.0 38.0 41.0 31.0 41.0 40-41 37.4861691853915 40.0 38.0 41.0 31.0 41.0 42-43 37.36786677943852 40.0 37.5 41.0 31.0 41.0 44-45 37.25721193319172 40.0 37.0 41.0 31.0 41.0 46-47 37.14969345505967 40.0 37.0 41.0 30.5 41.0 48-49 37.01131938789061 40.0 37.0 41.0 30.0 41.0 50-51 36.89792298608708 40.0 36.5 41.0 30.0 41.0 52-53 36.748859708033855 40.0 36.0 41.0 30.0 41.0 54-55 36.574558174047844 40.0 36.0 41.0 30.0 41.0 56-57 36.386238533420084 39.0 35.0 41.0 30.0 41.0 58-59 36.17083889220213 39.0 35.0 41.0 30.0 41.0 60-61 35.92098084861615 39.0 35.0 41.0 29.0 41.0 62-63 35.660127786950675 38.0 35.0 40.0 29.0 41.0 64-65 35.315591359721125 37.5 34.5 40.0 29.0 41.0 66-67 34.99666087100553 37.0 34.0 40.0 28.0 41.0 68-69 34.664164679911494 36.5 34.0 39.5 28.0 41.0 70-71 34.30028814891216 36.0 34.0 39.0 28.0 41.0 72-73 33.925223596225976 35.5 34.0 39.0 28.0 40.0 74-75 33.55867666636364 35.0 33.5 37.5 27.0 40.0 76-77 33.31187241105056 35.0 33.5 37.0 27.5 39.0 78-79 33.14470153931681 35.0 34.0 37.0 27.0 39.0 80-81 32.87350338910527 35.0 33.5 36.0 27.0 38.5 82-83 32.583726678334145 35.0 33.0 36.0 27.0 37.0 84-85 32.341547846094024 35.0 33.0 35.5 26.0 37.0 86-87 32.13192784187697 35.0 33.0 35.0 26.0 36.5 88-89 31.88263191237303 35.0 33.0 35.0 26.0 36.0 90-91 31.76295753873042 35.0 33.0 35.0 25.5 36.0 92-93 31.62339118047289 35.0 33.0 35.0 25.0 36.0 94-95 31.52682059993422 35.0 33.0 35.0 25.0 35.0 96-97 31.423975617290267 35.0 33.0 35.0 25.0 35.0 98-99 31.302262449684605 35.0 33.0 35.0 24.0 35.0 100-101 31.214271367355593 35.0 32.5 35.0 24.0 35.0 102-103 30.21172138751269 34.0 30.5 35.0 21.5 35.0 104-105 30.90713623103698 34.5 32.0 35.0 23.0 35.0 106-107 31.22988538298722 35.0 33.0 35.0 24.0 35.0 108-109 31.261790711743046 35.0 33.0 35.0 24.0 35.0 110-111 31.191007888159763 35.0 33.0 35.0 24.0 35.0 112-113 31.097756208124046 35.0 33.0 35.0 23.0 35.0 114-115 30.99711351325109 35.0 33.0 35.0 23.0 35.0 116-117 30.868052167613754 35.0 33.0 35.0 19.5 35.0 118-119 30.69392174286779 35.0 33.0 35.0 18.5 35.0 120-121 30.50856307743419 35.0 32.5 35.0 8.5 35.0 122-123 30.31882173082589 35.0 32.0 35.0 2.0 35.0 124-125 29.51104118663663 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1999518944527452 1101 2 0.18010717811367627 1101 3 0.24230683772868034 1101 4 0.3332231599074973 1101 5 0.21478279888058438 1101 6 0.21747585864281405 1101 7 0.29723990037708603 1101 8 0.11688712599255524 1101 9 0.27261245599271433 1101 10-11 0.3314640406820786 1101 12-13 0.3342919074604964 1101 14-15 0.43561388747652785 1101 16-17 0.44140302582545843 1101 18-19 0.2809278324549638 1101 20-21 0.38778718548910973 1101 22-23 0.4617054444433464 1101 24-25 0.44244028050793105 1101 26-27 0.4818121710091958 1101 28-29 0.49531784657512645 1101 30-31 0.5498735757408966 1101 32-33 0.4378719277461798 1101 34-35 0.5399391331460492 1101 36-37 0.4303874196596098 1101 38-39 0.5436891368061794 1101 40-41 0.46362068065280226 1101 42-43 0.6341333698932843 1101 44-45 0.5379118240060521 1101 46-47 0.6130816932412557 1101 48-49 0.4771461672632782 1101 50-51 0.47293220581999407 1101 52-53 0.4503744643724019 1101 54-55 0.45208754336619705 1101 56-57 0.3749141033810446 1101 58-59 0.44689048141147225 1101 60-61 0.3561233968260922 1101 62-63 0.44468178388616764 1101 64-65 0.6003658195298698 1101 66-67 0.45672763288479956 1101 68-69 0.35282278526474187 1101 70-71 0.3167271330346324 1101 72-73 0.44568198259452174 1101 74-75 0.42283489192758594 1101 76-77 0.42303395438020175 1101 78-79 0.36731429608486366 1101 80-81 0.40580297396726195 1101 82-83 0.37354376324631033 1101 84-85 0.3892685553923698 1101 86-87 0.39841495235133095 1101 88-89 0.397487918371489 1101 90-91 0.26723649685065354 1101 92-93 0.30457503125524 1101 94-95 0.3303808656240008 1101 96-97 0.3180644956997689 1101 98-99 0.17983733533181834 1101 100-101 0.088604167374708 1101 102-103 -0.12819699063873102 1101 104-105 -0.07211980610210489 1101 106-107 0.05505966900241788 1101 108-109 0.1104366421301961 1101 110-111 0.13891419937166916 1101 112-113 0.1631837903242399 1101 114-115 0.056211398720368067 1101 116-117 -0.06375757127027626 1101 118-119 -0.041443753463166644 1101 120-121 -0.17668275638576958 1101 122-123 -0.24808997886120565 1101 124-125 -0.3288441672042488 1102 1 0.1445901373467997 1102 2 0.09917559571558954 1102 3 0.21937209284843107 1102 4 0.18089082931241052 1102 5 0.21181411516737114 1102 6 0.2297111935959535 1102 7 0.22789090890035624 1102 8 0.1934577611886681 1102 9 0.24514803391704731 1102 10-11 0.2806878827122361 1102 12-13 0.20222404907799074 1102 14-15 0.2549098495267401 1102 16-17 0.35613363633877526 1102 18-19 0.30393826478941577 1102 20-21 0.3821313561088786 1102 22-23 0.4179981090274083 1102 24-25 0.3703916091361137 1102 26-27 0.3188416553703348 1102 28-29 0.25903511295640413 1102 30-31 0.39844322748219696 1102 32-33 0.48518429999347035 1102 34-35 0.3597244121772434 1102 36-37 0.2078566625922349 1102 38-39 0.3210851027130772 1102 40-41 0.24693328430122818 1102 42-43 0.22498724007724746 1102 44-45 0.171979855939405 1102 46-47 0.18627194354014875 1102 48-49 0.1461851776377685 1102 50-51 0.04908149811819129 1102 52-53 0.08629900831933668 1102 54-55 0.12742114666751547 1102 56-57 0.13710556853588685 1102 58-59 0.14849341634919 1102 60-61 0.14995414618010017 1102 62-63 0.17592382947219676 1102 64-65 0.0691555915997526 1102 66-67 0.08496869501600912 1102 68-69 0.0424830112665191 1102 70-71 0.02004147219155783 1102 72-73 0.05997100261072319 1102 74-75 0.050899992779910974 1102 76-77 0.0653167265657828 1102 78-79 0.1697107440774559 1102 80-81 0.18497301523379406 1102 82-83 0.14362841749444755 1102 84-85 0.1753830411033448 1102 86-87 0.1175113309235769 1102 88-89 0.20466665646397786 1102 90-91 0.10839118850864793 1102 92-93 0.15084127754317933 1102 94-95 0.058293627396533765 1102 96-97 -7.532373404579573E-4 1102 98-99 0.0634584738253956 1102 100-101 0.016507769519026994 1102 102-103 -0.10552498246255482 1102 104-105 -0.01311010653790845 1102 106-107 0.10127722463510835 1102 108-109 0.1400906974350633 1102 110-111 0.15291252171344638 1102 112-113 0.2028854756958296 1102 114-115 0.09673214768870508 1102 116-117 0.07333458423128292 1102 118-119 0.04260595921375909 1102 120-121 -0.09514663852545624 1102 122-123 -0.07597188714922609 1102 124-125 -0.02855780830108756 1103 1 0.2160787572186642 1103 2 0.22988335827266582 1103 3 0.2948959681094827 1103 4 0.29097100346553617 1103 5 0.24473772849079012 1103 6 0.20140246116170601 1103 7 0.16889299732200413 1103 8 0.14648597257941276 1103 9 0.36607738155075964 1103 10-11 0.4323506557114243 1103 12-13 0.3426106725603475 1103 14-15 0.40905041092279504 1103 16-17 0.3272054568053093 1103 18-19 0.4284360701768293 1103 20-21 0.45620837993104146 1103 22-23 0.4800158252144442 1103 24-25 0.5019710222542315 1103 26-27 0.4446491489103863 1103 28-29 0.34441704633929504 1103 30-31 0.5045390294007106 1103 32-33 0.5505735782491072 1103 34-35 0.4882353498431655 1103 36-37 0.4348751752112605 1103 38-39 0.31221815994999247 1103 40-41 0.4071599134470958 1103 42-43 0.3170282297767244 1103 44-45 0.20016469895382016 1103 46-47 0.1954132128757422 1103 48-49 0.2550153960371162 1103 50-51 0.1409499275543169 1103 52-53 0.12700331967114664 1103 54-55 0.23653675319472 1103 56-57 0.14161204618270062 1103 58-59 0.11036423508126347 1103 60-61 0.06647701262730266 1103 62-63 0.13919504111072456 1103 64-65 0.1281535031781047 1103 66-67 0.14009284991318083 1103 68-69 0.08152587700578096 1103 70-71 0.006066303170996434 1103 72-73 0.02740578368042179 1103 74-75 -0.05877754075871877 1103 76-77 0.033132025139288146 1103 78-79 0.12925863618747968 1103 80-81 0.06993283966478714 1103 82-83 0.06733465798151173 1103 84-85 -0.0380673677317418 1103 86-87 -0.0019977130040658153 1103 88-89 0.0758990907140884 1103 90-91 0.019049218312904515 1103 92-93 0.062125391379858286 1103 94-95 0.02032152021448752 1103 96-97 0.044408880814653884 1103 98-99 -0.10623242207209671 1103 100-101 -0.11667114450171923 1103 102-103 -0.2814899925506964 1103 104-105 -0.16181944426046613 1103 106-107 -0.15491282173493204 1103 108-109 -0.13605881939619735 1103 110-111 -0.15579115249277464 1103 112-113 -0.205502722952005 1103 114-115 -0.22444896094461697 1103 116-117 -0.22913922200566716 1103 118-119 -0.24711387371250737 1103 120-121 -0.3831806904174222 1103 122-123 -0.39722600204781244 1103 124-125 -0.39206263998846325 1104 1 0.2229824553263171 1104 2 0.2946764292839035 1104 3 0.35140218210867147 1104 4 0.33984946255718995 1104 5 0.3348843756595272 1104 6 0.35598772390700617 1104 7 0.29525122643134694 1104 8 0.28927936602616455 1104 9 0.4268958147784474 1104 10-11 0.48500838024781245 1104 12-13 0.41525589204724866 1104 14-15 0.4509309418557663 1104 16-17 0.4619786734329807 1104 18-19 0.3878814180686945 1104 20-21 0.4558135184065293 1104 22-23 0.39775202498459095 1104 24-25 0.4869345185286349 1104 26-27 0.49361324858239186 1104 28-29 0.5203923781927315 1104 30-31 0.5005181406212458 1104 32-33 0.4581868072944104 1104 34-35 0.38421604681212784 1104 36-37 0.3140974591240564 1104 38-39 0.2609964525990449 1104 40-41 0.36123963755940025 1104 42-43 0.28082889672625555 1104 44-45 0.2972966696142194 1104 46-47 0.3352745473074421 1104 48-49 0.3003123508105503 1104 50-51 0.22990955053652584 1104 52-53 0.18363202257162925 1104 54-55 0.1850525841482451 1104 56-57 0.10100519652201712 1104 58-59 0.11595364252571017 1104 60-61 0.07989493479674081 1104 62-63 0.2272915493536658 1104 64-65 0.2167965355519712 1104 66-67 0.21215825471714567 1104 68-69 0.10909756960723627 1104 70-71 0.1002459355335148 1104 72-73 0.061344205409398 1104 74-75 0.07495680734518118 1104 76-77 0.17572206667979628 1104 78-79 0.26246902658044036 1104 80-81 0.1593855185936235 1104 82-83 0.07208768477882899 1104 84-85 0.10683039520415605 1104 86-87 0.11167676496449985 1104 88-89 0.17382596860108634 1104 90-91 0.10425137989131628 1104 92-93 0.13200652936986046 1104 94-95 0.12844404896278405 1104 96-97 0.09353541805036514 1104 98-99 0.05269824880317486 1104 100-101 0.057726011885403494 1104 102-103 -0.15332004585827264 1104 104-105 0.05173126401000161 1104 106-107 0.013538387936993246 1104 108-109 0.026414982718257818 1104 110-111 -0.023945878033266865 1104 112-113 -0.007666171735280614 1104 114-115 -0.013396263881322312 1104 116-117 -0.0264426567977587 1104 118-119 -0.023175649185574798 1104 120-121 -0.08143563525879216 1104 122-123 -0.14446176807510724 1104 124-125 -0.23487400525077362 1105 1 0.12025103975188856 1105 2 0.07944542278774236 1105 3 0.04923693085280334 1105 4 0.20683746020090155 1105 5 0.17094748825422812 1105 6 0.23428284933040544 1105 7 0.2502472992800051 1105 8 0.26986901143712316 1105 9 0.34187830622871473 1105 10-11 0.23081583938088812 1105 12-13 0.3345486366351409 1105 14-15 0.4256413144442561 1105 16-17 0.3374109668893652 1105 18-19 0.24408560130303414 1105 20-21 0.27678450657597864 1105 22-23 0.2521004246704237 1105 24-25 0.2555374548924263 1105 26-27 0.2781501924404921 1105 28-29 0.3001303226562655 1105 30-31 0.24598596252492655 1105 32-33 0.31276827932163087 1105 34-35 0.27198961790175247 1105 36-37 0.2380271058440684 1105 38-39 0.17359330187825606 1105 40-41 0.21723242960037936 1105 42-43 0.2320906986341953 1105 44-45 0.22169164286049892 1105 46-47 0.18321340646997442 1105 48-49 0.20118542609731804 1105 50-51 0.21567362691962444 1105 52-53 0.31658746985780084 1105 54-55 0.22860878332026857 1105 56-57 0.16594054170226258 1105 58-59 0.22855826429194792 1105 60-61 0.10484134554404534 1105 62-63 0.09450239238215374 1105 64-65 0.2038802984174808 1105 66-67 0.10283045212194963 1105 68-69 0.057441518521535784 1105 70-71 0.13613171270040425 1105 72-73 0.021149197800546915 1105 74-75 0.03464204207428878 1105 76-77 -0.03317139706769012 1105 78-79 0.07064619428616226 1105 80-81 0.07407061014534122 1105 82-83 0.01650284603747565 1105 84-85 -0.011587740053478512 1105 86-87 0.028730521939280607 1105 88-89 0.1111974157563651 1105 90-91 0.06465485378393865 1105 92-93 0.005820406940916456 1105 94-95 -0.03757821716717302 1105 96-97 -0.11605337647780445 1105 98-99 -0.20490641387088715 1105 100-101 -0.30941774230764807 1105 102-103 -0.3764253998746021 1105 104-105 -0.23832005485832397 1105 106-107 -0.21513104409874373 1105 108-109 -0.16470208729958102 1105 110-111 -0.18615277345649872 1105 112-113 -0.2559273845705192 1105 114-115 -0.321574352013144 1105 116-117 -0.31508347380886903 1105 118-119 -0.31271480206630287 1105 120-121 -0.3336479305151201 1105 122-123 -0.4152645228081404 1105 124-125 -0.5191809448544582 1106 1 0.2508684911501966 1106 2 0.37104992449929597 1106 3 0.3033123684294985 1106 4 0.3300863460757313 1106 5 0.323375958188997 1106 6 0.2675612837423387 1106 7 0.2666733794970142 1106 8 0.2945315445379464 1106 9 0.40163255282175214 1106 10-11 0.46713753091353993 1106 12-13 0.42436165736754816 1106 14-15 0.46799986898794543 1106 16-17 0.4531479849091298 1106 18-19 0.4305227420380291 1106 20-21 0.3678763492421311 1106 22-23 0.46333734580974095 1106 24-25 0.3875568471290691 1106 26-27 0.4510232629048758 1106 28-29 0.4338535997989297 1106 30-31 0.5363875319882112 1106 32-33 0.4779026202116441 1106 34-35 0.48652693302156536 1106 36-37 0.4871196589668543 1106 38-39 0.4378205995595792 1106 40-41 0.45031629577618304 1106 42-43 0.40022106742576824 1106 44-45 0.31009989707479946 1106 46-47 0.3363111588435501 1106 48-49 0.2445277208722345 1106 50-51 0.2208567309692313 1106 52-53 0.333045439889446 1106 54-55 0.33471063696620007 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-0.5460096864620461 2216 98-99 -0.6323568149595573 2216 100-101 -0.5642836282838708 2216 102-103 -0.5987198419316115 2216 104-105 -0.5884480809290835 2216 106-107 -0.5705779714730461 2216 108-109 -0.5186323981432963 2216 110-111 -0.530906390416817 2216 112-113 -0.47593347252353624 2216 114-115 -0.4699610444640818 2216 116-117 -0.519849087617775 2216 118-119 -0.4497214988146183 2216 120-121 -0.2877913197143762 2216 122-123 -0.30931712988448723 2216 124-125 -0.3178988935724085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25313.0 3 5019.0 4 3194.0 5 2763.0 6 3155.0 7 3769.0 8 3993.0 9 4419.0 10 4952.0 11 5978.0 12 6414.0 13 6736.0 14 6574.0 15 6104.0 16 5417.0 17 5097.0 18 4629.0 19 4590.0 20 4578.0 21 4983.0 22 5933.0 23 8123.0 24 12053.0 25 17384.0 26 20453.0 27 21101.0 28 24834.0 29 30706.0 30 36618.0 31 45282.0 32 59231.0 33 79456.0 34 113628.0 35 174753.0 36 344620.0 37 685874.0 38 302484.0 39 787.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63021479140835 18.48930804672575 12.511309071446666 24.36916809041924 2 15.656777936499699 20.140574416808 39.491262651673715 24.711384995018584 3 18.562063380171075 25.141020142878833 29.310865940547593 26.9860505364025 4 12.218433868045008 16.193667139007744 37.55073461968134 34.03716437326591 5 13.20704773249124 39.03199130483219 34.48897559935196 13.271985363324617 6 32.293879540925104 36.55026333104488 17.874041015083808 13.281816112946213 7 28.91792154803899 32.607099049991696 21.162352464076605 17.312626937892713 8 26.655751203596388 35.300321990295075 20.107853659813564 17.93607314629497 9 27.154679421246197 15.749237147887406 19.15709541708056 37.93898801378583 10-11 24.838100441981272 26.86021473645332 27.942821350275153 20.358863471290256 12-13 25.306648928891434 24.16765529378587 27.884544218107997 22.6411515592147 14-15 22.593510061087855 25.61822626042336 25.009227790415995 26.779035888072794 16-17 22.799563253929573 28.883284657913975 25.792268604544898 22.524883483611553 18-19 22.866755307186523 27.87081171758816 27.01043386337572 22.251999111849596 20-21 23.75046793271669 27.116656310469818 26.686598478388706 22.446277278424787 22-23 23.42706819667044 27.11200920324969 26.546825150154902 22.914097449924963 24-25 23.03863356301794 27.210319672041415 26.696349399832553 23.05469736510809 26-27 22.963531828825037 27.558303881039308 26.513823321457224 22.964340968678435 28-29 22.984365934329023 27.491576586074462 26.655137175872884 22.868920303723627 30-31 23.050483223425093 27.17457680765542 26.624266121528134 23.150673847391353 32-33 22.90700903786278 27.20480705684856 26.579626933816343 23.30855697147232 34-35 23.382893479598234 27.382236644046298 26.23689154954884 22.997978326806624 36-37 23.42192070378066 27.09479863236261 26.375687144498883 23.107593519357845 38-39 23.052927103296607 27.068122771441228 26.578759048992772 23.300191076269392 40-41 23.17691085739444 26.87681387918776 26.669268617733326 23.27700664568447 42-43 22.957458652023973 26.814778535440254 26.700450908730904 23.52731190380487 44-45 23.03152093903359 27.00597367357007 26.79102518671746 23.171480200678875 46-47 23.275138684290063 27.305752750482508 26.339615277523272 23.07949328770416 48-49 23.13599210279691 27.057701653072325 26.52343149466658 23.282874749464185 50-51 23.062027218506262 27.171956932827605 26.603583917540103 23.162431931126033 52-53 23.21442893020783 26.943747743605524 26.536127181042502 23.305696145144143 54-55 22.882740303721913 26.997789079624074 26.638853653254387 23.48061696339963 56-57 22.977625849835032 27.343864529013857 26.525728749602568 23.152780871548543 58-59 23.05114392909032 26.87468838983666 26.494437721399393 23.579729959673628 60-61 22.81350533585089 26.973569915329872 26.9298759577092 23.28304879111004 62-63 22.790059324583147 27.02183833860705 26.893184336482335 23.294918000327467 64-65 22.80647435204837 26.930361114667672 26.662413069791196 23.600751463492763 66-67 22.71092939350957 27.231548735327998 26.568321798813866 23.489200072348567 68-69 22.809511003434185 26.91696792142546 26.87125018146682 23.40227089367353 70-71 22.800758509312157 26.85939733350849 26.752708162875788 23.587135994303566 72-73 22.78390848310915 27.04872639485413 26.507184503358133 23.660180618678588 74-75 22.86854377860005 27.259255063642396 26.360331810535953 23.5118693472216 76-77 22.96703461461849 27.04844628200268 26.58023542862093 23.404283674757902 78-79 23.06770197584379 26.98866536836541 26.580707160877896 23.362925494912897 80-81 22.866024456287633 27.142947577146398 26.57247380142439 23.41855416514158 82-83 23.073895198880145 27.17196520305437 26.52393706011788 23.230202537947605 84-85 23.123174221645773 26.972530774219265 26.562533094556727 23.341761909578235 86-87 23.011427189743326 26.96196584979966 26.610839647131733 23.41576731332528 88-89 23.138373575238543 26.925099886809228 26.53561986940698 23.400906668545247 90-91 23.019885863331098 27.0782345465709 26.61033607965769 23.291543510440317 92-93 23.051418459126396 26.736251753350942 26.712120123161746 23.500209664360913 94-95 22.952162528079178 26.830617257625434 26.725428175490613 23.491792038804775 96-97 23.1461504155416 26.73500102094581 26.712178479414256 23.40667008409833 98-99 23.094328828066637 26.801969837488173 26.410272702976766 23.69342863146842 100-101 22.857206093179727 26.689598909457146 26.75226038233732 23.70093461502581 102-103 22.821117974876827 26.423052035287537 26.91163622872023 23.84419376111541 104-105 22.79160743834007 26.72389235592256 26.682935269001913 23.80156493673546 106-107 22.842952012553006 26.8144607079558 26.466863555087066 23.87572372440413 108-109 23.00290033320395 26.86100747489666 26.25550014981207 23.88059204208732 110-111 22.807034662117655 27.04900788588879 26.50954226813545 23.634415183858106 112-113 22.872709306803074 27.02409913243885 26.594031167849547 23.50916039290853 114-115 22.770946848854738 27.22444807509011 26.37418682329478 23.630418252760368 116-117 22.722396189567768 27.288217960379235 26.49908209021647 23.490303759836532 118-119 22.66604032029379 27.47639951152602 26.215381406607925 23.642178761572268 120-121 22.695856510533005 27.535191539607094 26.379296761852356 23.38965518800754 122-123 22.70723162819921 27.601348939269172 26.2591686459677 23.43225078656392 124-125 22.833994106941798 27.724408974716873 26.126629116924207 23.314967801417122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1012.0 1 832.0 2 561.5 3 391.0 4 285.0 5 230.5 6 202.0 7 204.0 8 204.0 9 190.5 10 170.0 11 176.5 12 190.5 13 202.5 14 216.5 15 259.0 16 310.0 17 369.5 18 483.5 19 585.5 20 766.5 21 1188.5 22 1669.5 23 2394.0 24 3397.5 25 4935.0 26 7259.5 27 9960.5 28 12759.0 29 16118.5 30 20397.5 31 24856.0 32 29475.0 33 33242.0 34 36470.0 35 40835.0 36 47260.5 37 52823.0 38 55600.5 39 57854.5 40 60184.5 41 64580.5 42 68729.5 43 71364.5 44 76018.0 45 81491.5 46 83650.5 47 84110.5 48 83795.5 49 81931.0 50 81703.5 51 81241.0 52 80345.0 53 80050.5 54 80377.0 55 75758.5 56 65908.5 57 59039.5 58 54538.5 59 47575.5 60 38573.5 61 31490.0 62 26184.5 63 20532.5 64 15111.0 65 11286.0 66 8146.5 67 6365.0 68 4724.0 69 2972.0 70 2102.0 71 1414.5 72 1040.5 73 820.0 74 656.0 75 493.5 76 315.5 77 181.5 78 102.5 79 69.0 80 41.5 81 29.0 82 24.5 83 22.0 84 12.0 85 7.5 86 7.5 87 5.5 88 4.5 89 4.5 90 5.5 91 5.5 92 4.0 93 3.5 94 3.0 95 3.0 96 3.0 97 1.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043455559432022034 2 0.008948132719846815 3 0.0026654012356990514 4 0.005425995372673069 5 0.02475015433149119 6 0.02851027393185235 7 0.0032365586433488478 8 9.519290127496612E-5 9 0.0 10-11 1.4278935191244918E-4 12-13 9.281307874309196E-4 14-15 3.093769291436399E-4 16-17 2.379822531874153E-4 18-19 7.139467595622459E-5 20-21 2.379822531874153E-5 22-23 0.0 24-25 0.0 26-27 2.379822531874153E-5 28-29 3.807716050998645E-4 30-31 9.519290127496612E-5 32-33 1.1899112659370764E-4 34-35 6.901485342435044E-4 36-37 7.139467595622459E-5 38-39 1.665875772311907E-4 40-41 4.5216628105608904E-4 42-43 8.329378861559535E-4 44-45 0.0013564988431682672 46-47 9.519290127496612E-5 48-49 0.0 50-51 0.0 52-53 0.0011185165899808519 54-55 0.00295097993952395 56-57 4.759645063748306E-5 58-59 6.901485342435044E-4 60-61 9.519290127496611E-4 62-63 6.187538582872798E-4 64-65 0.0014278935191244918 66-67 0.0027129976863365345 68-69 0.00295097993952395 70-71 0.0016182793216744239 72-73 0.002451217207830378 74-75 0.0018562615748618392 76-77 9.757272380684027E-4 78-79 0.0022132349546429625 80-81 0.0011423148152995934 82-83 3.807716050998645E-4 84-85 4.5216628105608904E-4 86-87 0.0020466473774117715 88-89 0.005663977625860485 90-91 3.331751544623814E-4 92-93 9.519290127496611E-4 94-95 8.805343367934365E-4 96-97 1.9038580254993225E-4 98-99 5.473591823310552E-4 100-101 0.0011899112659370765 102-103 0.0013564988431682672 104-105 8.329378861559535E-4 106-107 0.006925283567753785 108-109 0.0014992881950807164 110-111 0.0013802970684870088 112-113 0.002379822531874153 114-115 0.002594006559742827 116-117 0.0013327006178495257 118-119 0.047001495004514525 120-121 0.011589735730227126 122-123 0.0041408912054610265 124-125 0.0024036207571928947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2100997.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.84495828997127 #Duplication Level Percentage of deduplicated Percentage of total 1 81.40907126369028 54.41785973046676 2 10.803257466651567 14.442865895082893 3 3.564363131152151 7.147791144965307 4 1.5400427775728471 4.1177638092651385 5 0.8216791020104565 2.746255265081501 6 0.47386155106629335 1.900515336974848 7 0.29394460921403814 1.3754100599731998 8 0.21292077056524633 1.1386144022001945 9 0.14498267507542376 0.8722224781366642 >10 0.6742595945636556 7.829815928983064 >50 0.04353620869944405 2.0207380050784667 >100 0.01808084973868719 1.990147943791911 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.759645063748306E-5 0.0 0.0 9.519290127496612E-5 0.0 3 4.759645063748306E-5 0.0 0.0 9.519290127496612E-5 0.0 4 4.759645063748306E-5 0.0 0.0 1.4278935191244918E-4 0.0 5 4.759645063748306E-5 0.0 0.0 1.4278935191244918E-4 0.0 6 4.759645063748306E-5 0.0 0.0 1.4278935191244918E-4 0.0 7 4.759645063748306E-5 0.0 0.0 3.331751544623814E-4 0.0 8 4.759645063748306E-5 0.0 0.0 3.331751544623814E-4 0.0 9 4.759645063748306E-5 0.0 0.0 3.807716050998645E-4 0.0 10-11 4.759645063748306E-5 0.0 0.0 4.04569830418606E-4 0.0 12-13 9.519290127496612E-5 0.0 0.0 7.139467595622459E-4 0.0 14-15 9.519290127496612E-5 0.0 0.0 7.139467595622459E-4 0.0 16-17 9.519290127496612E-5 0.0 0.0 8.329378861559536E-4 0.0 18-19 1.1899112659370764E-4 0.0 0.0 0.0010471219140246273 0.0 20-21 1.4278935191244918E-4 0.0 0.0 0.0011423148152995934 0.0 22-23 1.4278935191244918E-4 0.0 0.0 0.0015468846457181995 0.0 24-25 1.4278935191244918E-4 0.0 0.0 0.00202284915209303 0.0 26-27 1.4278935191244918E-4 0.0 0.0 0.0024512172078303774 0.0 28-29 1.4278935191244918E-4 0.0 0.0 0.005045223767573204 0.0 30-31 1.4278935191244918E-4 0.0 0.0 0.014516917444432334 0.0 32-33 1.4278935191244918E-4 0.0 0.0 0.03476920719068138 0.0 34-35 1.4278935191244918E-4 0.0 0.0 0.06361265627699611 0.0 36-37 1.4278935191244918E-4 0.0 0.0 0.11461225313505921 0.0 38-39 1.9038580254993225E-4 0.0 0.0 0.19505025471240559 0.0 40-41 1.9038580254993225E-4 0.0 0.0 0.2856263002755358 0.0 42-43 1.9038580254993225E-4 0.0 0.0 0.37401290910934193 0.0 44-45 1.9038580254993225E-4 0.0 0.0 0.47998640645369794 0.0 46-47 1.9038580254993225E-4 0.0 0.0 0.6120665569727135 0.0 48-49 1.9038580254993225E-4 0.0 0.0 0.7552366804902625 0.0 50-51 1.9038580254993225E-4 0.0 0.0 0.9140422380422247 0.0 52-53 1.9038580254993225E-4 0.0 0.0 1.065018179464321 0.0 54-55 1.9038580254993225E-4 0.0 0.0 1.2210869411046281 0.0 56-57 2.3798225318741531E-4 0.0 0.0 1.403666925750013 0.0 58-59 2.3798225318741531E-4 0.0 0.0 1.6165420512261561 0.0 60-61 2.3798225318741531E-4 0.0 0.0 1.8490507125902607 0.0 62-63 2.3798225318741531E-4 0.0 0.0 2.0745626957106555 0.0 64-65 2.3798225318741531E-4 0.0 0.0 2.3074283304545413 0.0 66-67 2.3798225318741531E-4 0.0 0.0 2.5703035273253603 0.0 68-69 2.3798225318741531E-4 0.0 0.0 2.863402470350981 0.0 70-71 2.3798225318741531E-4 0.0 0.0 3.195459108223382 0.0 72-73 2.3798225318741531E-4 0.0 0.0 3.5298955686276563 0.0 74-75 2.3798225318741531E-4 0.0 0.0 3.8631897142166314 0.0 76-77 2.3798225318741531E-4 0.0 0.0 4.224708555033634 0.0 78-79 2.3798225318741531E-4 0.0 0.0 4.624209363459348 0.0 80-81 2.3798225318741531E-4 0.0 0.0 5.056289942346419 0.0 82-83 2.3798225318741531E-4 0.0 0.0 5.51014589740014 0.0 84-85 2.617804785061568E-4 0.0 0.0 5.9533640457363814 0.0 86-87 2.8557870382489836E-4 0.0 0.0 6.416001545932717 0.0 88-89 2.8557870382489836E-4 0.0 0.0 6.914740953937583 0.0 90-91 3.331751544623814E-4 0.0 0.0 7.452890223070285 0.0 92-93 3.331751544623814E-4 0.0 0.0 8.014766322845773 0.0 94-95 3.331751544623814E-4 0.0 0.0 8.5845672316524 0.0 96-97 3.331751544623814E-4 0.0 0.0 9.147657040919146 0.0 98-99 3.331751544623814E-4 0.0 0.0 9.736091960150347 0.0 100-101 3.5697337978112296E-4 0.0 0.0 10.395683573084588 0.0 102-103 3.807716050998645E-4 0.0 0.0 11.093352346528814 0.0 104-105 4.2836805573734756E-4 0.0 0.0 11.786451860711843 0.0 106-107 4.2836805573734756E-4 0.0 0.0 12.462845020721115 0.0 108-109 4.2836805573734756E-4 0.0 0.0 13.171437179586643 0.0 110-111 4.2836805573734756E-4 0.0 0.0 13.92624549202117 0.0 112-113 4.2836805573734756E-4 0.0 0.0 14.71137274351177 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3040 0.0 52.097454 1 GGTATCA 1860 0.0 49.936726 1 ATCAACG 3410 0.0 45.034943 3 CAACGCA 3610 0.0 42.53892 5 TCAACGC 3605 0.0 42.433823 4 AACGCAG 3865 0.0 39.56892 6 TATCAAC 3985 0.0 39.283638 2 GTACATG 10295 0.0 38.401676 1 TACATGG 10675 0.0 36.13195 2 ACATGGG 10945 0.0 34.588017 3 ACGCAGA 4895 0.0 31.36071 7 CATGGGA 6995 0.0 31.059141 4 CGCAGAG 4925 0.0 31.04591 8 CATGGGG 4485 0.0 27.87027 4 ATGGGAT 3100 0.0 26.112663 5 ATGGGAG 2785 0.0 26.074057 5 AGTACAT 7540 0.0 24.630178 2 GAGTACA 7860 0.0 24.543184 1 GCAGAGT 6150 0.0 24.088058 9 TAAGGTG 940 0.0 24.061888 5 >>END_MODULE