FastQCFastQC Report
Thu 26 May 2016
SRR1364342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100997
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38610.1837698959113221No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35600.1694433642694397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.10076168599955164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34950.069.880681
GTATCAA59450.054.4087071
ATCAACG74400.043.38333
CAACGCA76550.042.087045
TCAACGC76600.042.0595664
AACGCAG79200.040.7249076
TATCAAC79850.040.7205852
GTACATG100050.036.199861
TACATGG103350.034.5729982
ACGCAGA93600.034.4521567
CGCAGAG94150.034.2508968
ACATGGG104950.033.478493
CATGGGA71100.029.985394
GCAGAGT110150.028.8976359
GAGTACT58650.027.64340212-13
GTGGTAT14300.027.4933931
GTACCGT2006.1114406E-826.7792596
TACCGTG1358.2524755E-526.4429867
CAGAGTA104000.026.28725210-11
CATGGGG44750.025.950034
Produced by FastQC (version 0.11.5)