FastQCFastQC Report
Thu 26 May 2016
SRR1364341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077925
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.053.278731
GTATCAA30850.045.159631
ATCAACG33750.039.8545533
TCAACGC33950.039.619774
CAACGCA34100.039.444545
GTACATG83800.038.9337461
TACATGG86300.036.8965952
AACGCAG36800.036.872196
ACATGGG88250.035.40693
TATCAAC38700.034.7568782
CATGGGA47550.031.6674614
ACGCAGA44400.030.4179127
CGCAGAG45050.029.4493478
CATGGGG45350.026.1167454
ATGGGAT24000.024.7982835
GTGTAAG11450.023.3989681
GAGTACA61850.023.3914011
CGTATAC1802.1732538E-523.1456183
GCAGAGT62500.021.036719
TAAGGTG12750.021.0056025
Produced by FastQC (version 0.11.5)