FastQCFastQC Report
Thu 26 May 2016
SRR1364341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077925
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.1604003994369383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29720.14302729886786097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30000.070.875181
GTATCAA53900.053.2605321
ATCAACG66700.042.1345023
TCAACGC67850.041.5081144
CAACGCA68100.041.2683035
TATCAAC70750.040.311672
AACGCAG70450.039.9531446
GTACATG80900.036.8102461
TATCACG1355.826041E-835.284012
TACATGG84000.034.590932
ACGCAGA83000.033.9063227
CGCAGAG84550.033.355118
ACATGGG84200.033.3066183
CATGGGA45600.028.8568674
CATGGGG42850.026.9570664
GCAGAGT103350.026.769499
CAGAGTA91700.025.85571910-11
AGAGTAC91300.025.05666210-11
GAGTACT60200.024.90586912-13
ATCACGC2201.5295336E-724.3579963
Produced by FastQC (version 0.11.5)