##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364341_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2077925 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30004307181443 33.0 31.0 34.0 30.0 34.0 2 32.318655870640185 33.0 31.0 34.0 30.0 34.0 3 32.42991542043144 34.0 31.0 34.0 30.0 34.0 4 36.031388043360565 37.0 35.0 37.0 35.0 37.0 5 35.97014569823261 37.0 35.0 37.0 35.0 37.0 6 36.024076903641856 37.0 35.0 37.0 35.0 37.0 7 35.97786493737743 37.0 35.0 37.0 35.0 37.0 8 35.98544990796107 37.0 35.0 37.0 35.0 37.0 9 37.74245557467185 39.0 38.0 39.0 35.0 39.0 10-11 37.697726818821664 39.0 37.5 39.0 35.0 39.0 12-13 37.67234885763442 39.0 37.0 39.0 35.0 39.0 14-15 39.07339124366857 41.0 38.0 41.0 36.0 41.0 16-17 39.0848315025807 41.0 38.0 41.0 36.0 41.0 18-19 39.136927463695756 41.0 38.5 41.0 36.0 41.0 20-21 39.13834666795 41.0 39.0 41.0 35.5 41.0 22-23 39.10159534150655 41.0 39.0 41.0 35.0 41.0 24-25 39.06556612967263 41.0 39.0 41.0 35.0 41.0 26-27 38.974927872757675 41.0 39.0 41.0 35.0 41.0 28-29 38.88865863782379 40.5 38.0 41.0 35.0 41.0 30-31 38.78417387538049 40.0 38.0 41.0 35.0 41.0 32-33 38.66306074569583 40.0 38.0 41.0 34.0 41.0 34-35 38.568988774859534 40.0 38.0 41.0 34.0 41.0 36-37 38.45538000649687 40.0 38.0 41.0 34.0 41.0 38-39 38.37325264386347 40.0 38.0 41.0 34.0 41.0 40-41 38.272705704007606 40.0 38.0 41.0 34.0 41.0 42-43 38.134495229616086 40.0 38.0 41.0 33.0 41.0 44-45 37.97859643634876 40.0 38.0 41.0 33.0 41.0 46-47 37.7690128373257 40.0 37.5 41.0 33.0 41.0 48-49 37.56932901813107 40.0 37.0 41.0 32.5 41.0 50-51 37.41874081114574 40.0 37.0 41.0 32.0 41.0 52-53 37.266455237797324 40.0 37.0 41.0 32.0 41.0 54-55 37.08926164322581 40.0 36.0 41.0 31.0 41.0 56-57 36.89302116293899 39.5 36.0 41.0 31.0 41.0 58-59 36.68104984539866 39.0 35.0 41.0 31.0 41.0 60-61 36.43570412791607 39.0 35.0 41.0 31.0 41.0 62-63 36.19430465488408 38.5 35.0 41.0 30.5 41.0 64-65 35.907083749413474 38.0 35.0 40.0 30.0 41.0 66-67 35.58780562339834 37.0 35.0 40.0 30.0 41.0 68-69 35.24206191272543 37.0 35.0 39.5 29.0 41.0 70-71 34.98753058941011 36.0 34.5 39.0 29.5 41.0 72-73 34.791173887411716 36.0 34.0 39.0 30.0 41.0 74-75 34.45114429057834 35.5 34.0 38.5 29.0 40.5 76-77 34.11975504409447 35.0 34.0 37.0 29.0 39.5 78-79 33.79639279569763 35.0 34.0 37.0 29.0 39.0 80-81 33.50090330497973 35.0 34.0 36.5 29.0 39.0 82-83 33.215718084146445 35.0 34.0 36.0 29.0 37.0 84-85 32.97588604016026 35.0 34.0 36.0 29.0 37.0 86-87 32.792283648351116 35.0 34.0 35.0 29.0 37.0 88-89 32.62855300359733 35.0 34.0 35.0 28.5 36.0 90-91 32.488256794638886 35.0 33.5 35.0 28.0 36.0 92-93 32.359062766942984 35.0 33.0 35.0 27.0 36.0 94-95 32.268655028453864 35.0 33.0 35.0 27.0 36.0 96-97 32.18449559055308 35.0 33.0 35.0 27.0 35.0 98-99 32.115826365244175 35.0 33.0 35.0 27.0 35.0 100-101 32.05930627910054 35.0 33.0 35.0 27.0 35.0 102-103 31.071657542981583 34.0 31.5 35.0 24.5 35.0 104-105 31.79169026793556 35.0 33.0 35.0 25.0 35.0 106-107 32.09239674194208 35.0 33.0 35.0 27.0 35.0 108-109 32.11582275587425 35.0 34.0 35.0 27.0 35.0 110-111 32.076859366916516 35.0 34.0 35.0 27.0 35.0 112-113 32.00378286030535 35.0 33.0 35.0 27.0 35.0 114-115 31.922533537062215 35.0 33.0 35.0 27.0 35.0 116-117 31.863433954546004 35.0 33.0 35.0 27.0 35.0 118-119 31.756659648447368 35.0 33.0 35.0 25.5 35.0 120-121 31.61575754659095 35.0 33.0 35.0 25.0 35.0 122-123 31.46412647232215 35.0 33.0 35.0 25.0 35.0 124-125 30.76113815464947 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09482332432569507 1101 2 0.08190897607479286 1101 3 0.11248698182271966 1101 4 0.03146076213711524 1101 5 0.15427633761071746 1101 6 0.2053514617316381 1101 7 0.19728527070357416 1101 8 0.20556494849922302 1101 9 0.1802824254982056 1101 10-11 0.26398609826644304 1101 12-13 0.17794367041645387 1101 14-15 0.2990657289677685 1101 16-17 0.2639670486831207 1101 18-19 0.1854331688129207 1101 20-21 0.20312867169150906 1101 22-23 0.18740588506512523 1101 24-25 0.2115492443831215 1101 26-27 0.151646029103496 1101 28-29 0.20075915916527265 1101 30-31 0.29204119872895973 1101 32-33 0.18204996679255459 1101 34-35 0.20053529418491678 1101 36-37 0.1907061784740165 1101 38-39 0.26409343210663394 1101 40-41 0.2466485018572726 1101 42-43 0.22880687743280248 1101 44-45 0.18420665084178012 1101 46-47 0.27716175067044446 1101 48-49 0.12086463782417667 1101 50-51 0.11406993202432147 1101 52-53 0.15922663394511716 1101 54-55 0.2207405318726856 1101 56-57 0.24624153945958938 1101 58-59 -0.008931205403428066 1101 60-61 0.13683880363079481 1101 62-63 0.07647774686162734 1101 64-65 -0.08488458850148817 1101 66-67 -0.050513232428620825 1101 68-69 0.01041255421500864 1101 70-71 0.09328823819879517 1101 72-73 7.921375437760503E-4 1101 74-75 0.026308569322779363 1101 76-77 -0.06490357659748014 1101 78-79 -0.20744181203975387 1101 80-81 -0.1667939653545787 1101 82-83 -0.2654335757815005 1101 84-85 -0.3995330351644597 1101 86-87 -0.3391742670043456 1101 88-89 -0.3688056295590343 1101 90-91 -0.7033894317030089 1101 92-93 -0.6323131885944733 1101 94-95 -0.5241094267967199 1101 96-97 -0.3547086378592397 1101 98-99 -0.21385031945911237 1101 100-101 -0.37699070340440244 1101 102-103 -0.23759650413040845 1101 104-105 -0.2651809859599119 1101 106-107 -0.2076047318704184 1101 108-109 -0.12038169957688183 1101 110-111 -0.22515723314585046 1101 112-113 -0.16557682705078847 1101 114-115 -0.30741540000333245 1101 116-117 -0.14003599221708285 1101 118-119 -0.1265473235251129 1101 120-121 -0.08699712695180395 1101 122-123 -0.13702959247897795 1101 124-125 -0.27304414583176495 1102 1 0.16887542615325657 1102 2 0.1931028971602018 1102 3 0.16461729601480357 1102 4 0.12767369639261972 1102 5 0.1752534290594312 1102 6 0.17090979970232922 1102 7 0.25283784510005347 1102 8 0.3127062598558936 1102 9 0.30426297950798187 1102 10-11 0.29086947311517264 1102 12-13 0.26616776083169214 1102 14-15 0.3661962419037934 1102 16-17 0.314506417649028 1102 18-19 0.37656184667991255 1102 20-21 0.36247129299155745 1102 22-23 0.3287181802484511 1102 24-25 0.3248216406167046 1102 26-27 0.3077083264950815 1102 28-29 0.3526150963091865 1102 30-31 0.40253686611109885 1102 32-33 0.39566249119389596 1102 34-35 0.33787657506751856 1102 36-37 0.368526907025128 1102 38-39 0.37309123267371547 1102 40-41 0.34963617531656865 1102 42-43 0.3189165509627543 1102 44-45 0.2886132456073085 1102 46-47 0.3504552027201555 1102 48-49 0.30630747244271816 1102 50-51 0.14275066976182416 1102 52-53 0.24459444272695663 1102 54-55 0.2507950318770966 1102 56-57 0.27113797178506616 1102 58-59 0.27555246888617546 1102 60-61 0.1788294229169054 1102 62-63 0.15364565678203945 1102 64-65 0.14331546493855285 1102 66-67 0.11795686985826848 1102 68-69 0.09378188228383522 1102 70-71 0.12991260553059192 1102 72-73 0.09772794766822557 1102 74-75 0.12176263297384082 1102 76-77 0.19106282684388276 1102 78-79 0.20984532216098017 1102 80-81 0.24079457506994117 1102 82-83 0.17130319228873248 1102 84-85 0.21153275128738613 1102 86-87 0.18872082490369024 1102 88-89 0.14252034583204676 1102 90-91 0.18582528683140964 1102 92-93 0.18756468325543096 1102 94-95 0.05101716205065543 1102 96-97 0.0857568425269406 1102 98-99 0.18017549296194346 1102 100-101 0.05915259318338428 1102 102-103 0.15244681100681134 1102 104-105 0.06743162456379892 1102 106-107 0.054275242248706945 1102 108-109 0.14126740033390206 1102 110-111 0.18536703128074805 1102 112-113 0.09655626955643726 1102 114-115 0.043857610128188895 1102 116-117 0.03684898362448763 1102 118-119 -0.00937474312214448 1102 120-121 0.053996349830487844 1102 122-123 -0.06138676625184658 1102 124-125 -0.25596613441944527 1103 1 0.04936441670252378 1103 2 0.13889364897004697 1103 3 0.09180886488682205 1103 4 0.11689877782816183 1103 5 0.03870922693633361 1103 6 0.02295800538632875 1103 7 0.043541425201624406 1103 8 0.11225075671631402 1103 9 0.2171898086428996 1103 10-11 0.27528131357205865 1103 12-13 0.20620074516467923 1103 14-15 0.30215911703086107 1103 16-17 0.28333638702149955 1103 18-19 0.2535856777511327 1103 20-21 0.19617447883410222 1103 22-23 0.30295441959703595 1103 24-25 0.2152338736921351 1103 26-27 0.2340546171520188 1103 28-29 0.3076020901361076 1103 30-31 0.2735562580772992 1103 32-33 0.2540219580857084 1103 34-35 0.19174487943111274 1103 36-37 0.21197277460549913 1103 38-39 0.2835993565421333 1103 40-41 0.20981650643945926 1103 42-43 0.22295084329515902 1103 44-45 0.19560601169250447 1103 46-47 0.1217363039331758 1103 48-49 0.057516688370348845 1103 50-51 -0.025759353767952575 1103 52-53 0.14262768625381028 1103 54-55 0.05073947471145601 1103 56-57 0.011696889995192805 1103 58-59 0.07671288090598694 1103 60-61 0.12152209719059925 1103 62-63 0.09085118603240261 1103 64-65 -0.04458297043824189 1103 66-67 -0.10441691940837217 1103 68-69 -0.03321376008769761 1103 70-71 -0.11116311462096462 1103 72-73 -0.04369615309584418 1103 74-75 -0.06995245938175287 1103 76-77 -0.08705207915459567 1103 78-79 -0.05866191257405973 1103 80-81 -0.03093667042218584 1103 82-83 -0.05302827619760819 1103 84-85 -0.04681178735026492 1103 86-87 4.76633662366055E-4 1103 88-89 0.01477730647231823 1103 90-91 0.07135734785461523 1103 92-93 0.10694844711122897 1103 94-95 0.09822790251940461 1103 96-97 -0.04376665088138765 1103 98-99 -0.049948356865286314 1103 100-101 -0.05345620398757234 1103 102-103 0.003418979382210807 1103 104-105 -0.07837285249070547 1103 106-107 -0.07685809658930509 1103 108-109 -0.08963785884082398 1103 110-111 -0.043539208893534465 1103 112-113 -0.0042891180231521275 1103 114-115 -0.0437608521303936 1103 116-117 -0.016475724495332855 1103 118-119 -0.015650460867686178 1103 120-121 0.011176648690852886 1103 122-123 -0.0035707862393010714 1103 124-125 -0.13519568339478383 1104 1 0.093279606480138 1104 2 0.11883933961806292 1104 3 0.1619316700922937 1104 4 0.1028756184022015 1104 5 0.18658469105962894 1104 6 0.19570513008069668 1104 7 0.16910975318473476 1104 8 0.12299661650692428 1104 9 0.19931145287976904 1104 10-11 0.2041552214712823 1104 12-13 0.18529042632312098 1104 14-15 0.2141155302502895 1104 16-17 0.23903440537489473 1104 18-19 0.2712481847834667 1104 20-21 0.18990268237104857 1104 22-23 0.14594570780425187 1104 24-25 0.17493813936764013 1104 26-27 0.11967522106701978 1104 28-29 0.18558561662997874 1104 30-31 0.1510218877787608 1104 32-33 0.1711220293093234 1104 34-35 0.1964977024457113 1104 36-37 0.19944578542791191 1104 38-39 0.1581392224657847 1104 40-41 0.018801538699250386 1104 42-43 -0.004568058563101829 1104 44-45 0.027027829492453748 1104 46-47 -0.038332726690391894 1104 48-49 -0.04522827783671346 1104 50-51 -0.03583680486398322 1104 52-53 -0.027857339429175454 1104 54-55 -4.881029504204548E-4 1104 56-57 -0.07015204306301825 1104 58-59 0.032323244405176865 1104 60-61 -0.012382175083267555 1104 62-63 -0.029650950136883125 1104 64-65 -0.0721485352988438 1104 66-67 -0.020973056143049007 1104 68-69 -0.003482430302099715 1104 70-71 0.014519768445296677 1104 72-73 0.1850385959837766 1104 74-75 0.07537228082888703 1104 76-77 0.02807761229384198 1104 78-79 0.0963498211009508 1104 80-81 0.13539908928662925 1104 82-83 0.10963880980355611 1104 84-85 0.2145961738668305 1104 86-87 0.20064161454541107 1104 88-89 0.16071245007425006 1104 90-91 0.16060033983284683 1104 92-93 0.13747093473227778 1104 94-95 0.10521161165450366 1104 96-97 0.04097230476870806 1104 98-99 -0.0424694463213271 1104 100-101 0.023675701150253303 1104 102-103 -0.006659819420683277 1104 104-105 0.029069465116574378 1104 106-107 0.010970018818383664 1104 108-109 0.08186743484391457 1104 110-111 0.09624543186280476 1104 112-113 0.06603266262322194 1104 114-115 0.029246908289156437 1104 116-117 0.04486574625608597 1104 118-119 -0.030397931868805017 1104 120-121 -0.0021109323305843475 1104 122-123 -0.03888997821033158 1104 124-125 -0.11984195993948532 1105 1 0.06930010315632273 1105 2 0.05476202639541583 1105 3 0.158927437508666 1105 4 0.04721734158002988 1105 5 0.043527717272567656 1105 6 0.06889235947848249 1105 7 0.09465456061082733 1105 8 0.12811578717628436 1105 9 0.06732078068350944 1105 10-11 0.03804110103664726 1105 12-13 0.07664805620957793 1105 14-15 0.1392771808725115 1105 16-17 0.15013291525470862 1105 18-19 0.14259465787689152 1105 20-21 0.10436390215404145 1105 22-23 0.13011220456083095 1105 24-25 0.11542772826990699 1105 26-27 0.11706289244624912 1105 28-29 0.016124562418212918 1105 30-31 0.07696271241510289 1105 32-33 0.06915004479345299 1105 34-35 0.05920411717476526 1105 36-37 0.02304849446470314 1105 38-39 0.045530640544789946 1105 40-41 0.031260669160836585 1105 42-43 1.603082210976936E-6 1105 44-45 -0.1370698104615684 1105 46-47 -0.12508876780120204 1105 48-49 -0.10113491149465403 1105 50-51 -0.04093488863219363 1105 52-53 -0.12193376335731898 1105 54-55 -0.20995918307252026 1105 56-57 -0.04213707958970048 1105 58-59 -0.12981633359439115 1105 60-61 -0.1009174820533616 1105 62-63 -0.06947291212880913 1105 64-65 -0.12681702942752082 1105 66-67 -0.19569110084477614 1105 68-69 -0.29434353395614465 1105 70-71 -0.2356291415233187 1105 72-73 -0.10888198272825633 1105 74-75 -0.05751185117907198 1105 76-77 -0.05557495763188314 1105 78-79 -0.04767319309089402 1105 80-81 -0.05838427357530662 1105 82-83 -0.09330501531658086 1105 84-85 -0.1690620817122479 1105 86-87 -0.13243343462091417 1105 88-89 -0.12771777644175586 1105 90-91 -0.10139352507643906 1105 92-93 -0.06769144065454924 1105 94-95 -0.1368745524434516 1105 96-97 -0.18028864401896527 1105 98-99 -0.1634537974456194 1105 100-101 -0.14436175293497655 1105 102-103 -0.09115248107494978 1105 104-105 -0.16238116581392248 1105 106-107 -0.16015992043675453 1105 108-109 -0.16867556587731514 1105 110-111 -0.18694108110100416 1105 112-113 -0.10251452173919517 1105 114-115 -0.2428252457758049 1105 116-117 -0.2286107433590594 1105 118-119 -0.27019640149086044 1105 120-121 -0.30355057932856866 1105 122-123 -0.26087610813188533 1105 124-125 -0.38019448294295444 1106 1 0.09522543819945639 1106 2 0.041230896411370566 1106 3 0.10357811049747312 1106 4 0.07174220603037895 1106 5 0.10070080046158836 1106 6 0.05302499541186023 1106 7 0.10256264394447356 1106 8 0.08567011966046323 1106 9 0.08237069965959876 1106 10-11 0.11024938898855652 1106 12-13 0.11755674353143775 1106 14-15 0.15990514721001858 1106 16-17 0.18174756129877068 1106 18-19 0.13099572580626528 1106 20-21 0.15054677825663276 1106 22-23 0.022352361885999983 1106 24-25 0.1805834736813665 1106 26-27 0.1354069810967573 1106 28-29 0.10817552116509432 1106 30-31 0.09894247150311486 1106 32-33 0.13228676835542075 1106 34-35 0.1693012017549833 1106 36-37 0.2331982478655732 1106 38-39 0.22263977939142876 1106 40-41 0.0864884527397507 1106 42-43 0.13415204220572718 1106 44-45 0.1580480740699528 1106 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0.5031097561496694 2202 38-39 0.5337391411773922 2202 40-41 0.5413416072536634 2202 42-43 0.5768632885667273 2202 44-45 0.5504906159450513 2202 46-47 0.5051163479782304 2202 48-49 0.5031421467058905 2202 50-51 0.5234397374413504 2202 52-53 0.5635200076943434 2202 54-55 0.5372909485257722 2202 56-57 0.43534337229858977 2202 58-59 0.49413020339463287 2202 60-61 0.46117634152069087 2202 62-63 0.4490145638927956 2202 64-65 0.4784380568925144 2202 66-67 0.5525034961656274 2202 68-69 0.5049545074325152 2202 70-71 0.3958075090830562 2202 72-73 0.38649773166824986 2202 74-75 0.32801809639164503 2202 76-77 0.26909860173221745 2202 78-79 0.2576570412136121 2202 80-81 0.29811815452417534 2202 82-83 0.2818204929689756 2202 84-85 0.3115527481107492 2202 86-87 0.26970453149201745 2202 88-89 0.2233781867007636 2202 90-91 0.30536938211227493 2202 92-93 0.26852222257204517 2202 94-95 0.21661399866746223 2202 96-97 0.33158676211685645 2202 98-99 0.33340646541317653 2202 100-101 0.3066921269031724 2202 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-0.555468648325018 2216 40-41 -0.83589405622876 2216 42-43 -0.6861403432209414 2216 44-45 -0.666398259545737 2216 46-47 -0.7740726429552467 2216 48-49 -0.7314335772457738 2216 50-51 -0.5809940619527154 2216 52-53 -0.6898307096774445 2216 54-55 -0.6585440321944915 2216 56-57 -0.6865752411832773 2216 58-59 -0.6398607126916289 2216 60-61 -0.7194100200093274 2216 62-63 -0.6510779603136427 2216 64-65 -0.6205681581524516 2216 66-67 -0.6987365369019187 2216 68-69 -0.8159227449463842 2216 70-71 -0.9036198959375952 2216 72-73 -0.8610352460227446 2216 74-75 -0.9249744471776324 2216 76-77 -0.8082283878570777 2216 78-79 -0.7120995435996704 2216 80-81 -0.6789869354279787 2216 82-83 -0.7263633967656773 2216 84-85 -0.6476109938402388 2216 86-87 -0.6631313586244758 2216 88-89 -0.6802090007450126 2216 90-91 -0.6292408548370361 2216 92-93 -0.6107563939398553 2216 94-95 -0.633380841774656 2216 96-97 -0.6686803947657793 2216 98-99 -0.65491795753762 2216 100-101 -0.6774402035821652 2216 102-103 -0.8088362044461945 2216 104-105 -0.7695524699509626 2216 106-107 -0.7500628273176417 2216 108-109 -0.5949677516085856 2216 110-111 -0.6124841775380148 2216 112-113 -0.5399991036469771 2216 114-115 -0.48037885965157656 2216 116-117 -0.5620956153636634 2216 118-119 -0.46752176776042376 2216 120-121 -0.3600864571063518 2216 122-123 -0.4166790895197785 2216 124-125 -0.42187392917241695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 338.0 3 13.0 4 18.0 5 104.0 6 281.0 7 640.0 8 1214.0 9 2110.0 10 3546.0 11 4629.0 12 6223.0 13 8250.0 14 12284.0 15 10148.0 16 5504.0 17 4772.0 18 4736.0 19 4868.0 20 4797.0 21 4951.0 22 5262.0 23 5845.0 24 7422.0 25 10182.0 26 13318.0 27 17725.0 28 23044.0 29 29317.0 30 37090.0 31 46326.0 32 59313.0 33 76783.0 34 106097.0 35 162022.0 36 323013.0 37 703490.0 38 370830.0 39 1420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64321625457073 19.423755673098587 13.440020641702786 24.493007430627898 2 15.636416136289807 20.869280652574083 38.92705463382942 24.56724857730669 3 18.185690051373367 25.788755609562426 30.219714378526657 25.805839960537558 4 12.411756920966829 17.06360912929966 38.2973880192981 32.22724593043541 5 12.610441883096405 38.83220634340522 35.25540370122657 13.301948072271811 6 31.315569580527963 38.09403705493549 17.377418938718613 13.212974425817928 7 27.584489334311872 32.93747368167764 22.03828338366399 17.4397536003465 8 26.213939386647738 35.05078383483523 20.418542536424557 18.316734242092473 9 26.70101182670212 15.059253822924312 20.172816631976612 38.06691771839696 10-11 24.33901608575863 26.494074617707568 28.722018359661682 20.44489093687212 12-13 25.48515947399449 24.051493677587015 28.451965301923792 22.0113815464947 14-15 22.377058844761 25.327237508572253 26.230253738705677 26.065449907961067 16-17 22.012247795276537 28.69800401843185 27.083917850740523 22.20583033555109 18-19 22.059506478819014 27.279377263375725 28.991157043685405 21.669959214119856 20-21 22.486471450664258 26.489872928247255 29.276192231608476 21.74746338948001 22-23 22.55993358759339 26.575429815801822 28.76961391773043 22.095022678874358 24-25 22.222029722446603 26.7065044952307 28.9515812533131 22.119884529009596 26-27 21.822692458546186 27.077430982499283 28.88424213694978 22.215634422004747 28-29 22.125723410229998 26.908658833346927 28.89531037911256 22.070307377310517 30-31 22.18579156079156 26.657787595287598 28.7478824978825 22.408538346038345 32-33 21.958732299912338 26.69117995611478 28.85211440957438 22.4979733343985 34-35 22.616325977665664 26.692562736369013 28.66097203383285 22.03013925213248 36-37 22.676877173141474 26.245629654583297 28.561882647352526 22.5156105249227 38-39 22.253064956627405 26.684240287787098 28.49575899033892 22.56693576524658 40-41 22.674599912797497 26.320646299524235 28.73423545253383 22.270518335144438 42-43 22.36623073498803 26.304727071477558 28.53536580964183 22.793676383892585 44-45 22.239039232695156 26.67486395666826 28.47719296557018 22.608903845066404 46-47 22.984467678092326 26.768579231685457 27.596977754250034 22.649975335972183 48-49 22.547053085322382 26.849939771473284 27.788739599570533 22.814267543633797 50-51 22.686814971666447 27.061154757751122 27.650901740919426 22.601128529663008 52-53 22.911943405079587 26.695742146612606 27.695104491259308 22.697209957048496 54-55 22.577210438297822 26.926429009709206 27.798236221230315 22.69812433076266 56-57 22.577520918725092 27.04270081230151 27.59926619032563 22.780512078647767 58-59 22.42800987909086 26.60256101763106 27.812903648064125 23.156525455213952 60-61 22.303409002588882 26.978963865145246 27.845405355199382 22.87222177706649 62-63 22.320680486543065 26.90306435506575 28.13554387189143 22.640711286499755 64-65 22.12952825535089 26.819808222144687 27.9254785422958 23.125184980208623 66-67 22.11254015424041 26.98949673352022 27.762888458438105 23.13507465380127 68-69 22.31983830022739 26.60969476761673 28.142088862687537 22.928378069468337 70-71 22.447489683217633 26.536857682543886 27.647196121130456 23.36845651310803 72-73 22.316726289490447 26.778873235708627 27.39561875112342 23.508781723677508 74-75 22.184005678742015 26.88848250057148 27.555470000120312 23.37204182056619 76-77 22.451363740269738 26.621220688908405 27.7328825631339 23.19453300768796 78-79 22.4848346307013 26.682748414885044 27.554651876270796 23.27776507814286 80-81 22.514287721787237 26.887794792919173 27.218028793690614 23.379888691602975 82-83 22.593765415017387 26.803614182417558 27.33431187362393 23.26830852894113 84-85 22.446508523288504 26.740697267874747 27.344111877019593 23.468682331817156 86-87 22.504253040894163 26.75368456513108 27.28120601080405 23.46085638317071 88-89 22.56618982879555 26.83535257528544 27.23493388837422 23.363523707544786 90-91 22.407666301719264 26.858308168004143 27.185894582335745 23.548130947940855 92-93 22.473669646402058 26.43317251585115 27.34150655100641 23.751651286740376 94-95 22.255880265168376 26.286247097465022 27.504409446924218 23.95346319044239 96-97 22.37195760193462 26.134244498718672 27.50424100966108 23.989556889685623 98-99 22.29675442815869 26.16609803007666 27.285220076677508 24.251927465087142 100-101 22.18987692048558 26.169303511916937 27.390425544714077 24.250394022883405 102-103 22.086384253522144 25.895087647532993 27.399785844051156 24.618742254893704 104-105 21.878271783596848 26.140677159795267 27.33767922847087 24.64337182813702 106-107 22.13165640321783 26.002298447873933 27.05678840997072 24.809256738937517 108-109 22.105884229834764 25.93561340612151 27.076403766011047 24.88209859803268 110-111 22.03419114391428 26.110933042262957 27.124505738629583 24.730370075193182 112-113 22.011646233622486 26.210426266588062 27.3204037681822 24.457523731607253 114-115 21.969729417568008 26.371981664400785 26.849356930591817 24.808931987439394 116-117 21.79960778180156 26.201523154108063 27.17783365617142 24.821035407918956 118-119 21.59471588243079 26.35484918849333 26.940192740353957 25.11024218872192 120-121 21.695415021588936 26.46998242469859 26.974502337919336 24.860100215793132 122-123 21.556682487833342 26.583554408571104 26.943916069613135 24.915847033982423 124-125 21.449447356807735 26.443520765687662 27.0245336328632 25.082498244641403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 40.0 1 35.5 2 98.0 3 169.0 4 128.0 5 109.0 6 225.0 7 552.0 8 964.0 9 1136.0 10 897.5 11 634.0 12 624.5 13 707.5 14 838.0 15 943.5 16 1054.5 17 1198.5 18 1378.5 19 1567.5 20 1807.5 21 2079.5 22 2507.5 23 3259.5 24 4283.5 25 5853.0 26 8299.5 27 11316.0 28 14604.0 29 18640.0 30 23057.5 31 28500.5 32 36316.5 33 41316.5 34 43383.5 35 47917.0 36 55029.5 37 61868.5 38 65259.5 39 67399.0 40 68666.5 41 69890.0 42 70856.0 43 71949.0 44 74869.5 45 76904.0 46 75690.5 47 75674.5 48 74360.0 49 71119.5 50 71125.0 51 69387.5 52 67137.0 53 67943.0 54 69979.0 55 66346.5 56 60449.5 57 55781.0 58 51334.5 59 45977.5 60 36585.0 61 29909.0 62 25657.0 63 19070.5 64 14087.5 65 11355.5 66 8484.0 67 6601.0 68 5102.5 69 3399.0 70 2201.5 71 1368.0 72 914.0 73 652.5 74 392.5 75 247.0 76 175.0 77 122.5 78 82.0 79 44.0 80 26.5 81 14.5 82 8.0 83 2.0 84 0.5 85 0.0 86 1.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02776808595112913 2 0.0 3 0.0 4 0.0 5 0.05553617190225826 6 0.017373100569077326 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.165621954594126E-4 22-23 0.0 24-25 2.406246616215696E-5 26-27 4.8124932324313923E-4 28-29 3.128120601080405E-4 30-31 2.4062466162156962E-4 32-33 2.165621954594126E-4 34-35 1.684372631350987E-4 36-37 0.0 38-39 0.0 40-41 1.4437479697294175E-4 42-43 0.0 44-45 1.2031233081078481E-4 46-47 0.0 48-49 2.887495939458835E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 9.624986464862784E-5 58-59 4.812493232431392E-5 60-61 2.406246616215696E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.218739848647088E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.165621954594126E-4 82-83 0.0 84-85 2.406246616215696E-5 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.218739848647088E-5 100-101 0.0 102-103 0.0 104-105 0.0012271857742700049 106-107 4.812493232431392E-5 108-109 0.0011309359096213771 110-111 0.0037056197889721717 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.331243909188252E-4 122-123 6.01561654053924E-4 124-125 9.624986464862784E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2077925.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.44249900857251 #Duplication Level Percentage of deduplicated Percentage of total 1 78.89337885603955 42.16259321296828 2 11.468023297009857 12.257596473614715 3 3.887191782962139 6.232237290212559 4 1.8382749412714319 3.9296802690552872 5 1.003011553319058 2.680172197192027 6 0.6120063937104684 1.9624290653467047 7 0.4032252053784442 1.5084553847068265 8 0.27703558771978215 1.184437929764301 9 0.1958536532049518 0.9420217800507847 >10 1.2078972151795269 12.576807930684462 >50 0.12868712763411677 4.756323835714718 >100 0.08203386995393339 7.765990722893498 >500 0.0024790455189803305 0.9174080136700997 >1k 9.014710978110292E-4 1.123845894125732 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3333 0.1604003994369383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2972 0.14302729886786097 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.624986464862784E-5 2 0.0 0.0 0.0 0.0 9.624986464862784E-5 3 0.0 0.0 0.0 0.0 9.624986464862784E-5 4 0.0 0.0 0.0 0.0 1.4437479697294175E-4 5 0.0 0.0 0.0 0.0 1.4437479697294175E-4 6 0.0 0.0 0.0 0.0 2.406246616215696E-4 7 0.0 0.0 0.0 0.0 2.406246616215696E-4 8 0.0 0.0 0.0 0.0 2.406246616215696E-4 9 0.0 0.0 0.0 0.0 2.406246616215696E-4 10-11 0.0 0.0 0.0 0.0 2.406246616215696E-4 12-13 0.0 0.0 0.0 0.0 4.3312439091882526E-4 14-15 0.0 0.0 0.0 0.0 6.01561654053924E-4 16-17 0.0 0.0 0.0 0.0 6.737490525403948E-4 18-19 0.0 0.0 0.0 0.0 6.737490525403948E-4 20-21 0.0 0.0 0.0 0.0 6.978115187025518E-4 22-23 0.0 0.0 0.0 4.812493232431392E-5 7.699989171890227E-4 24-25 0.0 0.0 0.0 1.4437479697294175E-4 8.181238495133367E-4 26-27 0.0 0.0 0.0 7.699989171890227E-4 9.865611126484352E-4 28-29 0.0 0.0 0.0 0.0035371825258370734 0.0010346860449727494 30-31 0.0 0.0 0.0 0.013499043516970052 0.0010828109772970632 32-33 0.0 0.0 0.0 0.037729946942262116 0.001203123308107848 34-35 0.0 0.0 0.0 0.06862615349447165 0.0012993731727564757 36-37 0.0 0.0 0.0 0.11853170831478518 0.0012993731727564757 38-39 0.0 0.0 0.0 0.19890034529638942 0.0012993731727564757 40-41 0.0 0.0 0.0 0.28665615938977584 0.0012993731727564757 42-43 0.0 0.0 0.0 0.37828603053526955 0.0013715605712429466 44-45 0.0 0.0 0.0 0.485821191813949 0.0014918729020537316 46-47 0.0 0.0 0.0 0.6187181924275419 0.0015640603005402025 48-49 0.0 0.0 0.0 0.7571976851907551 0.001684372631350987 50-51 0.0 0.0 0.0 0.9141812144326672 0.001828747428323929 52-53 0.0 0.0 0.0 1.0657988137204182 0.001828747428323929 54-55 0.0 0.0 0.0 1.2286295222397343 0.0019249972929725567 56-57 0.0 0.0 0.0 1.4212255014016386 0.0019971846914590276 58-59 0.0 0.0 0.0 1.6324458293730526 0.0020212471576211847 60-61 0.0 0.0 0.0 1.8584164491018684 0.0020212471576211847 62-63 0.0 0.0 0.0 2.07964676299674 0.0021656219545941264 64-65 0.0 0.0 0.0 2.3187314267839314 0.002237809353080597 66-67 0.0 0.0 0.0 2.5888085469879805 0.002309996751567068 68-69 0.0 0.0 0.0 2.8974818629161305 0.002406246616215696 70-71 0.0 0.0 0.0 3.222156718842114 0.002406246616215696 72-73 0.0 0.0 0.0 3.5554700001203123 0.0026709337439994223 74-75 0.0 0.0 0.0 3.8925370261198067 0.002863433473296678 76-77 0.0 0.0 0.0 4.2594896350927005 0.0028874959394588353 78-79 0.0 0.0 0.0 4.675120613111638 0.002935620871783149 80-81 0.0 0.0 0.0 5.113057497262894 0.00300780827026962 82-83 0.0 0.0 0.0 5.579412153951658 0.0032243704657290324 84-85 0.0 0.0 0.0 6.04696993394853 0.0032724953980533466 86-87 0.0 0.0 0.0 6.533344562484209 0.003368745262701974 88-89 0.0 0.0 0.0 7.0517222710155565 0.003489057593512759 90-91 0.0 0.0 0.0 7.611944608202895 0.0035131200596749163 92-93 0.0 0.0 0.0 8.191007856395203 0.0037056197889721717 94-95 0.0 0.0 0.0 8.78012921544329 0.003753744721296486 96-97 0.0 0.0 0.0 9.382099931421973 0.004186869112215311 98-99 0.0 0.0 0.0 10.006256241202161 0.004523743638485509 100-101 0.0 0.0 0.0 10.681376854313799 0.004523743638485509 102-103 0.0 0.0 0.0 11.388091485496348 0.004812493232431392 104-105 0.0 0.0 0.0 12.093819555566249 0.005053117894052961 106-107 0.0 0.0 0.0 12.802122309515504 0.005077180360215119 108-109 0.0 0.0 0.0 13.542428143460423 0.00514936775870159 110-111 0.0 0.0 0.0 14.323880794542633 0.005341867487998845 112-113 0.0 4.812493232431392E-5 0.0 15.129660598914782 0.005389992420323159 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3000 0.0 70.87518 1 GTATCAA 5390 0.0 53.260532 1 ATCAACG 6670 0.0 42.134502 3 TCAACGC 6785 0.0 41.508114 4 CAACGCA 6810 0.0 41.268303 5 TATCAAC 7075 0.0 40.31167 2 AACGCAG 7045 0.0 39.953144 6 GTACATG 8090 0.0 36.810246 1 TATCACG 135 5.826041E-8 35.28401 2 TACATGG 8400 0.0 34.59093 2 ACGCAGA 8300 0.0 33.906322 7 CGCAGAG 8455 0.0 33.35511 8 ACATGGG 8420 0.0 33.306618 3 CATGGGA 4560 0.0 28.856867 4 CATGGGG 4285 0.0 26.957066 4 GCAGAGT 10335 0.0 26.76949 9 CAGAGTA 9170 0.0 25.855719 10-11 AGAGTAC 9130 0.0 25.056662 10-11 GAGTACT 6020 0.0 24.905869 12-13 ATCACGC 220 1.5295336E-7 24.357996 3 >>END_MODULE