FastQCFastQC Report
Thu 26 May 2016
SRR1364340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445538
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.054.225381
GTATCAA29050.052.6753541
ATCAACG31800.046.415673
TCAACGC31950.046.197764
CAACGCA32500.045.415955
AACGCAG33800.043.4885836
TATCAAC36150.041.3243032
GTACATG84700.038.944561
TACATGG88100.036.6829262
ACATGGG87050.035.1426433
ACGCAGA42000.034.989487
CGCAGAG43400.033.585458
CATGGGA64450.030.3817674
GCAGAGT51050.027.7367789
CATGGGG26900.027.6565364
ATACCGT1150.001003885225.8742146
ATGGGAG27150.025.8673675
GAGTACA61050.024.6748961
ATGGGAT28200.024.2710635
AGTACAT62950.022.974742
Produced by FastQC (version 0.11.5)