##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364340_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1445538 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.125890844792735 31.0 31.0 34.0 28.0 34.0 2 31.19856067429566 33.0 31.0 34.0 28.0 34.0 3 31.37440039625385 33.0 31.0 34.0 28.0 34.0 4 34.76509507186944 37.0 35.0 37.0 32.0 37.0 5 34.89626561183449 37.0 35.0 37.0 32.0 37.0 6 34.940529408427864 37.0 35.0 37.0 32.0 37.0 7 34.843614626526595 37.0 35.0 37.0 32.0 37.0 8 34.83345232017422 37.0 35.0 37.0 32.0 37.0 9 36.37334542571693 39.0 37.0 39.0 32.0 39.0 10-11 36.30834021658372 39.0 37.0 39.0 32.0 39.0 12-13 36.3327134949064 39.0 37.0 39.0 32.0 39.0 14-15 37.67295740409453 40.0 38.0 41.0 32.0 41.0 16-17 37.69447707358783 40.0 38.0 41.0 32.0 41.0 18-19 37.70595999551724 40.0 38.0 41.0 32.0 41.0 20-21 37.74487076783869 40.0 38.0 41.0 32.0 41.0 22-23 37.7230633162186 40.0 38.0 41.0 32.0 41.0 24-25 37.74914599270306 40.0 38.0 41.0 32.0 41.0 26-27 37.730444651057255 40.0 38.0 41.0 32.0 41.0 28-29 37.74830478340936 40.0 38.0 41.0 32.0 41.0 30-31 37.66163220890769 40.0 38.0 41.0 31.5 41.0 32-33 37.62850959296816 40.0 38.0 41.0 31.0 41.0 34-35 37.621531222285405 40.0 38.0 41.0 31.0 41.0 36-37 37.56368148052836 40.0 38.0 41.0 31.0 41.0 38-39 37.47608744979378 40.0 38.0 41.0 31.0 41.0 40-41 37.41636574064466 40.0 38.0 41.0 31.0 41.0 42-43 37.29725749167438 40.0 37.5 41.0 30.0 41.0 44-45 37.17578991351317 40.0 37.0 41.0 30.0 41.0 46-47 37.076152269950704 40.0 37.0 41.0 30.0 41.0 48-49 36.94472853705679 40.0 37.0 41.0 30.0 41.0 50-51 36.83616860988781 40.0 36.5 41.0 30.0 41.0 52-53 36.69293508714402 40.0 36.0 41.0 30.0 41.0 54-55 36.520575730281735 40.0 36.0 41.0 30.0 41.0 56-57 36.3393663812366 39.0 35.0 41.0 30.0 41.0 58-59 36.13220579465915 39.0 35.0 41.0 29.5 41.0 60-61 35.89850214937276 39.0 35.0 41.0 29.0 41.0 62-63 35.64035466379991 38.5 35.0 40.5 29.0 41.0 64-65 35.302382573131936 38.0 34.5 40.0 28.5 41.0 66-67 34.988993717218094 37.0 34.0 40.0 28.0 41.0 68-69 34.66117736095488 37.0 34.0 39.5 28.0 41.0 70-71 34.29408324098017 36.0 34.0 39.0 28.0 41.0 72-73 33.91961781703421 36.0 34.0 39.0 27.0 40.5 74-75 33.54527414706497 35.0 33.5 38.0 26.5 40.0 76-77 33.29939268286272 35.0 33.5 37.0 26.5 39.0 78-79 33.119833238558925 35.0 34.0 37.0 27.0 39.0 80-81 32.834118162234404 35.0 33.5 36.5 27.0 39.0 82-83 32.53027142835401 35.0 33.0 36.0 26.0 37.0 84-85 32.27678137828269 35.0 33.0 36.0 26.0 37.0 86-87 32.06030453713427 35.0 33.0 35.0 26.0 37.0 88-89 31.78774269510729 35.0 33.0 35.0 25.0 36.0 90-91 31.664554996132928 35.0 33.0 35.0 25.0 36.0 92-93 31.51615834381386 35.0 33.0 35.0 25.0 36.0 94-95 31.41592334480311 35.0 33.0 35.0 25.0 35.5 96-97 31.312236689730746 35.0 33.0 35.0 24.5 35.0 98-99 31.178296938579273 35.0 32.5 35.0 24.0 35.0 100-101 31.091143228334364 35.0 32.0 35.0 23.5 35.0 102-103 30.096577537221435 34.0 30.5 35.0 20.0 35.0 104-105 30.781587201443337 34.5 32.0 35.0 21.5 35.0 106-107 31.098328788312728 35.0 33.0 35.0 23.5 35.0 108-109 31.128619586617578 35.0 33.0 35.0 23.5 35.0 110-111 31.05539183335201 35.0 33.0 35.0 23.0 35.0 112-113 30.961631932194102 35.0 33.0 35.0 21.5 35.0 114-115 30.863193842015914 35.0 33.0 35.0 19.5 35.0 116-117 30.726809672246596 35.0 33.0 35.0 18.5 35.0 118-119 30.557078748535147 35.0 32.5 35.0 13.5 35.0 120-121 30.372524969941985 35.0 32.0 35.0 2.0 35.0 122-123 30.18501589027753 35.0 32.0 35.0 2.0 35.0 124-125 29.37807964923786 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.050520698036876865 1101 2 -0.018838993569477935 1101 3 0.0626829543495191 1101 4 0.15752401723047882 1101 5 0.1925858229275903 1101 6 0.03542240398765273 1101 7 0.21500116242311407 1101 8 0.08520555243112682 1101 9 0.20801629947754208 1101 10-11 0.29413235629432677 1101 12-13 0.34910233070507957 1101 14-15 0.38983810557363796 1101 16-17 0.351292068452814 1101 18-19 0.18181640650941233 1101 20-21 0.2836102500802511 1101 22-23 0.3835904862741444 1101 24-25 0.4458340145029851 1101 26-27 0.5064675935912106 1101 28-29 0.4072577858318951 1101 30-31 0.503874183446797 1101 32-33 0.4168740247237466 1101 34-35 0.4382507638432216 1101 36-37 0.34116253015574216 1101 38-39 0.4477373762792425 1101 40-41 0.29646372188054215 1101 42-43 0.3646323200832242 1101 44-45 0.37902275014450737 1101 46-47 0.35177731738926354 1101 48-49 0.28231723739623504 1101 50-51 0.24751638777328822 1101 52-53 0.1697131720257019 1101 54-55 0.19299314602631057 1101 56-57 0.13079041743528563 1101 58-59 0.17331488315549137 1101 60-61 0.19374313115917374 1101 62-63 0.21035664885916106 1101 64-65 0.2627186633635432 1101 66-67 0.19934492339123722 1101 68-69 0.17641276340101086 1101 70-71 0.09754173381702458 1101 72-73 0.16925913220973143 1101 74-75 0.23849072213462819 1101 76-77 0.3110836394795342 1101 78-79 0.3316494505800023 1101 80-81 0.31569785407793916 1101 82-83 0.20673089628204622 1101 84-85 0.10796861333859908 1101 86-87 0.11828042500653169 1101 88-89 0.2032613254188682 1101 90-91 0.10434722041982525 1101 92-93 0.07878226055876425 1101 94-95 0.011692558709437861 1101 96-97 0.0857749699224577 1101 98-99 -0.06086172127041323 1101 100-101 -0.10292207063648462 1101 102-103 -0.40141965181680916 1101 104-105 -0.20766724013005344 1101 106-107 -0.1386103476961651 1101 108-109 -0.040091463738427535 1101 110-111 -0.19507285136965535 1101 112-113 -0.15063057230234733 1101 114-115 -0.2527232021776733 1101 116-117 -0.31880286990177353 1101 118-119 -0.24864101742997136 1101 120-121 -0.2961836914891727 1101 122-123 -0.3679732897416663 1101 124-125 -0.5299994229673146 1102 1 0.21526589436101773 1102 2 0.28227549477905356 1102 3 0.32416645032349933 1102 4 0.4563384291467898 1102 5 0.4846350950440552 1102 6 0.4667596122565527 1102 7 0.39399474567201764 1102 8 0.43082847498573784 1102 9 0.5666042587373923 1102 10-11 0.5401338849386264 1102 12-13 0.5446283798705451 1102 14-15 0.5248618351102365 1102 16-17 0.5749167329509461 1102 18-19 0.5885744363357475 1102 20-21 0.5510501263667109 1102 22-23 0.6109194469737673 1102 24-25 0.5913485099614917 1102 26-27 0.527056299398275 1102 28-29 0.5524203376013901 1102 30-31 0.6385939616156264 1102 32-33 0.49732159755654237 1102 34-35 0.4304102405491719 1102 36-37 0.3523145236145666 1102 38-39 0.47275737307975163 1102 40-41 0.3923968170297627 1102 42-43 0.31465453875046023 1102 44-45 0.27979418227092623 1102 46-47 0.36814092143484345 1102 48-49 0.33067572226088515 1102 50-51 0.30558887395328327 1102 52-53 0.34262061794546383 1102 54-55 0.37704725292476127 1102 56-57 0.29650968013103096 1102 58-59 0.2927517732530731 1102 60-61 0.2401854781614361 1102 62-63 0.3305809685235701 1102 64-65 0.33955970213067843 1102 66-67 0.24820110640195736 1102 68-69 0.24577766501675313 1102 70-71 0.20923586275640105 1102 72-73 0.30727616059630236 1102 74-75 0.22464938119362188 1102 76-77 0.3262118856268188 1102 78-79 0.4053443260665972 1102 80-81 0.3298147064926624 1102 82-83 0.4363154960794162 1102 84-85 0.3510283904409235 1102 86-87 0.2124413592570491 1102 88-89 0.35335819880801367 1102 90-91 0.26799748082260777 1102 92-93 0.31266661683953956 1102 94-95 0.3077891832495112 1102 96-97 0.22897339150833673 1102 98-99 0.18137329890413767 1102 100-101 0.2380313768119251 1102 102-103 0.04649801468988102 1102 104-105 0.1992880914613373 1102 106-107 0.197031949536278 1102 108-109 0.2588367077541349 1102 110-111 0.17282028572840957 1102 112-113 0.15097094923198284 1102 114-115 0.059875670891525345 1102 116-117 -0.05895111284957011 1102 118-119 0.03367825593073448 1102 120-121 -0.07080997350626816 1102 122-123 -0.09735525529384503 1102 124-125 -0.03645350142587134 1103 1 0.26173054082566694 1103 2 0.3515270040692471 1103 3 0.35750955252539995 1103 4 0.42456420723717514 1103 5 0.3652985965973059 1103 6 0.41600057116869493 1103 7 0.2873480074972292 1103 8 0.3618876157160571 1103 9 0.5221832595775169 1103 10-11 0.597838591579503 1103 12-13 0.4355726387064891 1103 14-15 0.5738439190052844 1103 16-17 0.4189508849154677 1103 18-19 0.47225358761645 1103 20-21 0.4920383451112187 1103 22-23 0.5555182395416125 1103 24-25 0.5648084478314885 1103 26-27 0.5336571238907908 1103 28-29 0.4766637387306716 1103 30-31 0.6189897961700694 1103 32-33 0.4899392635784565 1103 34-35 0.6421299521721693 1103 36-37 0.523689784931797 1103 38-39 0.4617175747092048 1103 40-41 0.4699176735641615 1103 42-43 0.4181517936983923 1103 44-45 0.39034651409985344 1103 46-47 0.36302789562549265 1103 48-49 0.40931413735964384 1103 50-51 0.32447600444582747 1103 52-53 0.35582031315676943 1103 54-55 0.40323465889940735 1103 56-57 0.33244641693817556 1103 58-59 0.3934268020712466 1103 60-61 0.23575138871767365 1103 62-63 0.2709654711904719 1103 64-65 0.3331806309546934 1103 66-67 0.3278134776971129 1103 68-69 0.28542461810664577 1103 70-71 0.3698468077967476 1103 72-73 0.45175186542016377 1103 74-75 0.28022349759945797 1103 76-77 0.19529243971705057 1103 78-79 0.34259692947006215 1103 80-81 0.4308296919370491 1103 82-83 0.37618517937269047 1103 84-85 0.25588937944117873 1103 86-87 0.25906523827938344 1103 88-89 0.3173022818043272 1103 90-91 0.14212658618962593 1103 92-93 0.2403535441396194 1103 94-95 0.16539327054411856 1103 96-97 0.22904568113574797 1103 98-99 0.2542490584336683 1103 100-101 0.18123322117430973 1103 102-103 -0.14481843803856265 1103 104-105 0.05634609900290499 1103 106-107 0.0393555726142516 1103 108-109 0.08639175532852406 1103 110-111 0.1211488323161447 1103 112-113 0.08183178193034024 1103 114-115 0.006476104629289381 1103 116-117 -0.029296735419563902 1103 118-119 -0.11406318165596119 1103 120-121 -0.2156500571277853 1103 122-123 -0.27373217731873467 1103 124-125 -0.3419045300877315 1104 1 0.20494954715827518 1104 2 0.33498455548548733 1104 3 0.31356893507974704 1104 4 0.3739760870965867 1104 5 0.3486498961541358 1104 6 0.2524087689182153 1104 7 0.3773600751326569 1104 8 0.2796847377717526 1104 9 0.4597676976082141 1104 10-11 0.5180930293655024 1104 12-13 0.41373305196826493 1104 14-15 0.4673463924197776 1104 16-17 0.49002951515512905 1104 18-19 0.5022240174019998 1104 20-21 0.4477815478899956 1104 22-23 0.382122932396463 1104 24-25 0.5104042760615144 1104 26-27 0.476899634422594 1104 28-29 0.4306904910170175 1104 30-31 0.4260428589926022 1104 32-33 0.46408110272731307 1104 34-35 0.4028607460216733 1104 36-37 0.43415166498439817 1104 38-39 0.2784997661248312 1104 40-41 0.41585511762094995 1104 42-43 0.2983660232049843 1104 44-45 0.24595023716500464 1104 46-47 0.32715404813619386 1104 48-49 0.25507732836700114 1104 50-51 0.23507504598966023 1104 52-53 0.20269077732928764 1104 54-55 0.14938834158884617 1104 56-57 0.2683292153416872 1104 58-59 0.24813060408474286 1104 60-61 0.17947042274015956 1104 62-63 0.2589912086105741 1104 64-65 0.2886759127281877 1104 66-67 0.2156128020640793 1104 68-69 0.13089996316760732 1104 70-71 0.10178656750011328 1104 72-73 0.0045500242364369115 1104 74-75 -0.016221443974892225 1104 76-77 -0.035575786082958416 1104 78-79 0.11810928881514116 1104 80-81 0.1404423942502504 1104 82-83 0.11710289922067574 1104 84-85 0.019883393275819117 1104 86-87 0.010230145028891968 1104 88-89 0.11585873760290255 1104 90-91 0.06668680008427685 1104 92-93 0.05208684038139211 1104 94-95 0.03127798792061398 1104 96-97 0.028663045651750707 1104 98-99 -0.011589874050677906 1104 100-101 -0.05834991092444142 1104 102-103 -0.23371743695286185 1104 104-105 -0.003902568985150623 1104 106-107 -0.06373416706225399 1104 108-109 -0.07899763519792913 1104 110-111 -0.22157458958616516 1104 112-113 -0.13652834619008303 1104 114-115 -0.13179008396840075 1104 116-117 -0.20407460463833615 1104 118-119 -0.1445146425868309 1104 120-121 -0.36121478254078454 1104 122-123 -0.3279412320377979 1104 124-125 -0.4264372215310459 1105 1 0.10579189102616837 1105 2 0.08314242655655235 1105 3 0.04183889331015678 1105 4 0.26779560342652786 1105 5 0.27701654502615725 1105 6 0.23404583842641102 1105 7 0.2975711617734689 1105 8 0.3109841872856549 1105 9 0.3481722828876386 1105 10-11 0.29649638368886144 1105 12-13 0.31716231695993713 1105 14-15 0.4147711749455638 1105 16-17 0.34401261470248556 1105 18-19 0.4153309568689423 1105 20-21 0.41400137112980673 1105 22-23 0.36108538313430927 1105 24-25 0.49642399559487416 1105 26-27 0.5081850523130456 1105 28-29 0.4413105165673272 1105 30-31 0.4672597961825886 1105 32-33 0.44020246861656176 1105 34-35 0.3055810951284599 1105 36-37 0.293503136104448 1105 38-39 0.3804155929669335 1105 40-41 0.34788593361724907 1105 42-43 0.32491194137383417 1105 44-45 0.23380841827117393 1105 46-47 0.2201881124533287 1105 48-49 0.2515636822942966 1105 50-51 0.21229566201620287 1105 52-53 0.20877125379219308 1105 54-55 0.2749015322715991 1105 56-57 0.2449075656947457 1105 58-59 0.23986119436325737 1105 60-61 0.19218945159875034 1105 62-63 0.12050514424496583 1105 64-65 0.025238879319530838 1105 66-67 0.12133783199288928 1105 68-69 0.19005601204102618 1105 70-71 0.19608481731020788 1105 72-73 0.19717646989226978 1105 74-75 0.11083426858602508 1105 76-77 0.2391162038372272 1105 78-79 0.3119450052921593 1105 80-81 0.251149033185321 1105 82-83 0.2831617414714245 1105 84-85 0.22409932210231887 1105 86-87 0.265145199493908 1105 88-89 0.34901999220862123 1105 90-91 0.26464940835898076 1105 92-93 0.15903746883867242 1105 94-95 0.1325499545865796 1105 96-97 0.10340567697658187 1105 98-99 0.06888180534968313 1105 100-101 -0.08580150660704078 1105 102-103 -0.09155700064590988 1105 104-105 0.05743412173299589 1105 106-107 -0.012256605222656702 1105 108-109 -0.04695993095011275 1105 110-111 -0.0899751822309156 1105 112-113 -0.09646582892959543 1105 114-115 0.022628888827465943 1105 116-117 -0.04927263230231915 1105 118-119 -0.027731991796311917 1105 120-121 -0.07290892886637934 1105 122-123 -0.10214466634141672 1105 124-125 -0.1114253616202241 1106 1 0.010240925572006176 1106 2 0.1555323279928551 1106 3 0.06786515141521932 1106 4 0.16448383249898768 1106 5 0.1032501241300352 1106 6 0.09775942631876688 1106 7 0.15113010838351926 1106 8 0.0762477647033819 1106 9 0.27606915633879936 1106 10-11 0.3095909465902835 1106 12-13 0.12062347908164384 1106 14-15 0.21223433491102384 1106 16-17 0.1984820236779612 1106 18-19 0.24348286197391644 1106 20-21 0.15833357524710578 1106 22-23 0.30393383058999035 1106 24-25 0.22714684714639333 1106 26-27 0.39158136115618447 1106 28-29 0.2957103075857148 1106 30-31 0.3342559665562561 1106 32-33 0.3046594996470091 1106 34-35 0.26883494902528327 1106 36-37 0.29305349351920995 1106 38-39 0.26652380865231606 1106 40-41 0.33143246396046777 1106 42-43 0.19089002896216556 1106 44-45 0.26228216272089355 1106 46-47 0.1767963255839078 1106 48-49 0.13674227470861666 1106 50-51 0.17249526755701794 1106 52-53 0.22998780523794693 1106 54-55 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19110.0 3 3654.0 4 2450.0 5 1937.0 6 2153.0 7 2604.0 8 2982.0 9 3182.0 10 3547.0 11 4037.0 12 4785.0 13 5002.0 14 4994.0 15 4431.0 16 4195.0 17 3752.0 18 3530.0 19 3293.0 20 3365.0 21 3580.0 22 4194.0 23 5677.0 24 8468.0 25 12822.0 26 15124.0 27 14390.0 28 17050.0 29 20878.0 30 25055.0 31 30484.0 32 39722.0 33 53385.0 34 76268.0 35 116875.0 36 230438.0 37 467001.0 38 220474.0 39 650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25654491570821 19.170965808499357 12.939021166035234 23.633468109757196 2 15.251677411231078 20.49146869132174 40.22521381890739 24.03164007853979 3 18.364523254599277 26.052456625705034 29.49811795096365 26.084902168732043 4 11.988698352696352 16.885156418091768 38.30197176940378 32.8241734598081 5 12.878527345861016 39.53492073267353 34.58921527042091 12.997336651044547 6 31.055211633291673 37.72391276968834 18.046853699482526 13.174021897537461 7 28.333277988549167 33.19040542821643 21.377199609265823 17.09911697396858 8 26.259277732542575 35.99777521201551 20.110464597438586 17.63248245800332 9 26.892963035215956 16.463074647639843 19.70989347910605 36.93406883803816 10-11 24.37588094393954 27.700561209930562 27.99903150212291 19.92452634400699 12-13 24.93670969266109 25.2260170464431 27.913593813720517 21.923679447175292 14-15 22.212406522175257 26.459187979495407 25.344060655953182 25.98434484237615 16-17 22.233275407876384 29.895208420959424 26.06261621986585 21.80889995129834 18-19 22.325136722594 28.69367186508262 27.463229050252274 21.517962362071106 20-21 23.26577484153818 27.8823275079339 27.21219615540932 21.639701495118597 22-23 22.910293606947725 27.977368979577154 26.95615749983743 22.15617991363769 24-25 22.591450380412 28.063253958041916 27.09129749615714 22.253998165388943 26-27 22.33451813029907 28.285770814424545 26.95705952670843 22.42265152856795 28-29 22.359299924837284 28.15997310330014 27.14027825770539 22.34044871415719 30-31 22.395468524250735 27.889391702094763 27.201721717276012 22.51341805637849 32-33 22.216976188425544 28.105101462329042 27.011355299572166 22.666567049673255 34-35 22.815425846164917 27.975015254005125 26.822040757594834 22.387518142235123 36-37 22.91189263509248 27.6092111065953 26.809577752220193 22.669318506092022 38-39 22.41599548955143 27.84769626417946 27.029727799271203 22.706580446997908 40-41 22.625309143736704 27.556804621158403 27.260822192628975 22.557064042475915 42-43 22.613988470577002 27.509185282241187 27.06218320201948 22.814643045162324 44-45 22.333916749105853 27.814404604603222 27.22578882193828 22.62588982435265 46-47 22.771050164817872 27.974452365387208 26.676939679772545 22.57755779002238 48-49 22.496399264495295 27.810476099556013 26.871967392071326 22.821157243877366 50-51 22.553125549103516 27.71542498363931 27.03069722138055 22.70075224587662 52-53 22.790532769519462 27.678487733589296 26.900286230662136 22.630693266229105 54-55 22.334276154465858 27.716001105511683 27.102296842339022 22.84742589768344 56-57 22.40161351857943 27.97048016428503 27.041344165297797 22.586562151837743 58-59 22.511151634558445 27.452633224676383 27.029950301896612 23.006264838868564 60-61 22.275282657504576 27.728089827788793 27.267736937845616 22.728890576861016 62-63 22.286009186291334 27.70259355866577 27.281673386838744 22.72972386820415 64-65 22.20986597837584 27.659350002386695 27.100932333365385 23.029851685872075 66-67 22.20427375645669 27.78813369662258 26.97509125757136 23.03250128934937 68-69 22.343523594041155 27.52543010754548 27.242306456360737 22.88873984205263 70-71 22.2862712827649 27.563625621884093 27.128034360274413 23.022068735076598 72-73 22.353872261412842 27.602059911490773 26.97314214676039 23.070925680335993 74-75 22.239886212540696 27.885744883092517 26.90283375602386 22.971535148342927 76-77 22.45478855783775 27.610361300672736 27.0596919364641 22.87515820502541 78-79 22.481523528056634 27.657564543991057 27.044899054782228 22.81601287317008 80-81 22.459880285088214 27.939163655922535 26.850729928900897 22.75022613008836 82-83 22.471081521021024 27.816979112836894 26.954422913687655 22.75751645245443 84-85 22.5182736249642 27.651456978290256 26.957834034116303 22.872435362629243 86-87 22.586498706155698 27.628980590432988 27.03039816367848 22.754122539732833 88-89 22.65936136114183 27.602999377003506 26.922338523356487 22.815300738498173 90-91 22.588797107770617 27.757045260608837 26.977918456813544 22.676239174807 92-93 22.593769154033364 27.403567440875864 27.093782298110437 22.908881106980335 94-95 22.38183820609636 27.367663356665222 27.183127432519 23.067371004719416 96-97 22.64480847855112 27.304933138710368 27.131086867792437 22.919171514946076 98-99 22.59642357817897 27.302505736678008 26.891754748973206 23.209315936169816 100-101 22.379947631121087 27.30314109504225 27.21874210921367 23.098169164622988 102-103 22.40191156189868 27.204786929543324 27.254734370584416 23.138567137973578 104-105 22.321163591082822 27.409314954774217 27.04851178637519 23.221009667767767 106-107 22.35912173836941 27.422827980865122 27.015092808439878 23.202957472325586 108-109 22.47359569644615 27.412741447094884 26.798111107344642 23.31555174911432 110-111 22.392961122147167 27.691489777515205 26.861024223555003 23.054524876782626 112-113 22.39866233276133 27.686550307972702 27.104537767688235 22.810249591577733 114-115 22.378262902873207 27.791644644176515 26.82968843826185 23.000404014688424 116-117 22.231023412819614 27.862178889617965 26.9150055188077 22.991792178754718 118-119 22.259432151940175 28.032285314528494 26.616073928290003 23.09220860524133 120-121 22.223848115295883 28.16019128804979 26.71027519451043 22.905685402143895 122-123 22.35116117699881 28.29475196008504 26.608971596148805 22.74511526676735 124-125 22.414319706372414 28.236508430101743 26.598256871562835 22.750914991963015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 722.0 1 607.0 2 440.0 3 326.0 4 247.0 5 215.5 6 183.0 7 172.5 8 177.0 9 183.0 10 180.0 11 165.0 12 162.0 13 176.5 14 196.0 15 229.5 16 262.0 17 326.5 18 423.5 19 545.0 20 742.0 21 1040.0 22 1508.5 23 2246.0 24 3094.5 25 4285.5 26 6003.5 27 8134.0 28 10221.5 29 12885.0 30 16799.5 31 20314.5 32 24301.0 33 27724.5 34 30345.0 35 33461.0 36 37313.0 37 41490.5 38 43796.0 39 45832.0 40 47490.0 41 49394.5 42 51234.5 43 53618.0 44 55826.5 45 57109.5 46 57287.0 47 56004.0 48 55395.5 49 54139.0 50 52607.0 51 50429.5 52 48822.5 53 48249.5 54 47294.0 55 44373.5 56 39409.0 57 35352.5 58 32838.5 59 28895.5 60 23309.0 61 18919.0 62 15671.5 63 12093.0 64 8880.5 65 6719.0 66 4927.5 67 3828.0 68 2681.0 69 1634.5 70 1167.0 71 786.5 72 556.5 73 421.5 74 311.5 75 247.0 76 169.0 77 90.0 78 55.5 79 41.0 80 26.5 81 23.5 82 19.0 83 11.5 84 9.0 85 5.5 86 7.5 87 11.0 88 8.0 89 6.0 90 4.5 91 3.0 92 1.5 93 1.0 94 1.0 95 1.5 96 1.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0467645956038513 2 0.010791829754734915 3 0.0026979574386837286 4 0.005880163648413255 5 0.025250114490245154 6 0.030715207763476297 7 0.003735633376639009 8 4.8424877104579747E-4 9 0.0 10-11 3.458919793184267E-5 12-13 0.0011414435317508083 14-15 5.534271669094828E-4 16-17 1.383567917273707E-4 18-19 1.0376759379552803E-4 20-21 3.458919793184267E-5 22-23 0.0 24-25 0.0 26-27 1.383567917273707E-4 28-29 5.188379689776402E-4 30-31 3.113027813865841E-4 32-33 1.0376759379552803E-4 34-35 0.0011068543338189655 36-37 3.458919793184267E-5 38-39 3.804811772502694E-4 40-41 8.993191462279095E-4 42-43 0.0013143895214100217 44-45 0.0019369950841831899 46-47 2.0753518759105606E-4 48-49 0.0 50-51 0.0 52-53 0.0017640490945239765 54-55 0.003701044178707167 56-57 1.0376759379552803E-4 58-59 6.917839586368536E-4 60-61 0.0011414435317508083 62-63 0.0010030867400234375 64-65 0.001729459896592134 66-67 0.0028709034283429424 68-69 0.003908579366298222 70-71 0.002179119469706089 72-73 0.0026979574386837286 74-75 0.0024904222510926726 76-77 0.001694870698660291 78-79 0.003528098189047953 80-81 0.0014181571152055497 82-83 6.226055627731682E-4 84-85 8.301407503642243E-4 86-87 0.0027325466366155717 88-89 0.007229142367755119 90-91 6.571947607050109E-4 92-93 0.0013835679172737071 94-95 0.0013835679172737071 96-97 5.534271669094828E-4 98-99 0.0010376759379552803 100-101 0.001591103104864763 102-103 0.001279800323478179 104-105 8.993191462279095E-4 106-107 0.007920926326391972 108-109 0.0024212438552289875 110-111 0.001625692302796606 112-113 0.0025250114490245157 114-115 0.003182206209729526 116-117 0.001833227490387662 118-119 0.05067317497014952 120-121 0.012348343661667836 122-123 0.004496595731139548 124-125 0.0023174762614334593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1445538.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.48326541259553 #Duplication Level Percentage of deduplicated Percentage of total 1 84.06955045251654 57.573573367572806 2 9.774743914544308 13.388127636797817 3 2.8555566751345998 5.866735370518548 4 1.143770283664764 3.1331649562901487 5 0.5823699558454791 1.9941298127243743 6 0.3506700706039099 1.4409018910452704 7 0.22878639454781638 1.0967627566426237 8 0.1445544638041838 0.7919649369021884 9 0.10715893209045242 0.6604734228912679 >10 0.6567200423947359 8.611464567654616 >50 0.06294908073937429 2.933406871228952 >100 0.023169734113864127 2.5092944097313494 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.458919793184268E-5 0.0 12-13 0.0 0.0 0.0 1.3835679172737072E-4 0.0 14-15 0.0 0.0 0.0 1.3835679172737072E-4 0.0 16-17 0.0 0.0 0.0 1.3835679172737072E-4 0.0 18-19 0.0 0.0 0.0 1.3835679172737072E-4 0.0 20-21 0.0 0.0 0.0 1.3835679172737072E-4 0.0 22-23 0.0 0.0 0.0 1.729459896592134E-4 0.0 24-25 0.0 0.0 0.0 3.113027813865841E-4 0.0 26-27 0.0 0.0 0.0 9.684975420915949E-4 0.0 28-29 0.0 0.0 0.0 0.003666454980775324 0.0 30-31 0.0 0.0 0.0 0.012106219276144938 0.0 32-33 0.0 0.0 0.0 0.03199500808695448 0.0 34-35 0.0 0.0 0.0 0.0594934204427694 0.0 36-37 0.0 0.0 0.0 0.10930186546462285 0.0 38-39 0.0 0.0 0.0 0.18826900434301969 0.0 40-41 0.0 0.0 0.0 0.2771286538299236 0.0 42-43 0.0 0.0 0.0 0.3633595242740073 0.0 44-45 0.0 0.0 0.0 0.46816479400749067 0.0 46-47 0.0 0.0 0.0 0.5982201782312191 0.0 48-49 0.0 0.0 0.0 0.7397937653662512 0.0 50-51 0.0 0.0 0.0 0.8926088418291321 0.0 52-53 0.0 0.0 0.0 1.036430726829734 0.0 54-55 0.0 0.0 0.0 1.1911482091788663 0.0 56-57 0.0 0.0 0.0 1.3731565686962224 0.0 58-59 0.0 0.0 0.0 1.5810376482665969 0.0 60-61 0.0 0.0 0.0 1.8064554511884157 0.0 62-63 0.0 0.0 0.0 2.032530448870939 0.0 64-65 0.0 0.0 0.0 2.2565300946775526 0.0 66-67 0.0 0.0 0.0 2.5223826699816954 0.0 68-69 0.0 0.0 0.0 2.820195664174861 0.0 70-71 0.0 0.0 0.0 3.1444002163900224 0.0 72-73 0.0 0.0 0.0 3.477701727661258 0.0 74-75 0.0 0.0 0.0 3.8020792258660787 0.0 76-77 0.0 0.0 0.0 4.150150324654212 0.0 78-79 0.0 0.0 0.0 4.541457920857148 0.0 80-81 0.0 0.0 0.0 4.965037238730494 0.0 82-83 0.0 0.0 0.0 5.420992045868044 0.0 84-85 0.0 0.0 0.0 5.862350211478356 0.0 86-87 0.0 0.0 0.0 6.307893670038421 0.0 88-89 0.0 0.0 0.0 6.794944166116698 0.0 90-91 0.0 0.0 0.0 7.332702426363056 0.0 92-93 0.0 0.0 0.0 7.89553785511 0.0 94-95 0.0 0.0 0.0 8.460310278941128 0.0 96-97 0.0 0.0 0.0 9.011316201995381 0.0 98-99 0.0 0.0 0.0 9.5879181315192 0.0 100-101 0.0 0.0 0.0 10.231899818614245 0.0 102-103 0.0 0.0 0.0 10.903518274856836 0.0 104-105 0.0 3.458919793184268E-5 0.0 11.574099055161469 0.0 106-107 0.0 6.917839586368536E-5 0.0 12.21759649348547 0.0 108-109 0.0 6.917839586368536E-5 0.0 12.885479316351422 0.0 110-111 0.0 6.917839586368536E-5 0.0 13.597913026153584 0.0 112-113 0.0 6.917839586368536E-5 0.0 14.360258948571396 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1680 0.0 54.22538 1 GTATCAA 2905 0.0 52.675354 1 ATCAACG 3180 0.0 46.41567 3 TCAACGC 3195 0.0 46.19776 4 CAACGCA 3250 0.0 45.41595 5 AACGCAG 3380 0.0 43.488583 6 TATCAAC 3615 0.0 41.324303 2 GTACATG 8470 0.0 38.94456 1 TACATGG 8810 0.0 36.682926 2 ACATGGG 8705 0.0 35.142643 3 ACGCAGA 4200 0.0 34.98948 7 CGCAGAG 4340 0.0 33.58545 8 CATGGGA 6445 0.0 30.381767 4 GCAGAGT 5105 0.0 27.736778 9 CATGGGG 2690 0.0 27.656536 4 ATACCGT 115 0.0010038852 25.874214 6 ATGGGAG 2715 0.0 25.867367 5 GAGTACA 6105 0.0 24.674896 1 ATGGGAT 2820 0.0 24.271063 5 AGTACAT 6295 0.0 22.97474 2 >>END_MODULE