FastQCFastQC Report
Thu 26 May 2016
SRR1364340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445538
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.24212438552289875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33270.23015652303848116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.13240744968309376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.074.06331
GTATCAA46800.056.0034981
ATCAACG58800.044.356243
TCAACGC59250.044.019364
CAACGCA60750.042.8344425
TATCAAC61400.042.4779622
AACGCAG63000.041.3719446
GTACATG74600.035.3731081
ACGCAGA75050.034.802557
CGCAGAG75900.034.2560238
TGGTATC11700.032.572572
ACATGGG77150.032.5712553
TACATGG79800.032.3104482
CATGGGA56150.030.4361764
GCAGAGT86200.029.748659
ATAGCGC850.00721257227.9987288
GTGGTAT14550.026.6108461
GAGTACT48350.026.08775112-13
CAGAGTA84300.025.23165310-11
AGAGTAC84500.024.92549910-11
Produced by FastQC (version 0.11.5)