##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364340_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1445538 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35308999140804 33.0 31.0 34.0 30.0 34.0 2 32.36572335006067 34.0 31.0 34.0 30.0 34.0 3 32.473003131014195 34.0 31.0 34.0 30.0 34.0 4 36.066378054399124 37.0 35.0 37.0 35.0 37.0 5 36.00558338833016 37.0 35.0 37.0 35.0 37.0 6 36.06782042395288 37.0 35.0 37.0 35.0 37.0 7 36.028833555395984 37.0 35.0 37.0 35.0 37.0 8 36.04183632668252 37.0 35.0 37.0 35.0 37.0 9 37.800141538997934 39.0 38.0 39.0 35.0 39.0 10-11 37.75936156642025 39.0 38.0 39.0 35.0 39.0 12-13 37.73316474558261 39.0 38.0 39.0 35.0 39.0 14-15 39.143899018911995 41.0 39.0 41.0 36.0 41.0 16-17 39.156411315371855 41.0 39.0 41.0 36.0 41.0 18-19 39.21870023479148 41.0 39.0 41.0 36.0 41.0 20-21 39.226080531954196 41.0 39.0 41.0 36.0 41.0 22-23 39.18839214188766 41.0 39.0 41.0 36.0 41.0 24-25 39.15482228761886 41.0 39.0 41.0 35.0 41.0 26-27 39.06740673714562 41.0 39.0 41.0 35.0 41.0 28-29 38.98070891252945 41.0 39.0 41.0 35.0 41.0 30-31 38.86643069915838 40.5 38.0 41.0 35.0 41.0 32-33 38.73646490095729 40.0 38.0 41.0 35.0 41.0 34-35 38.64043560252307 40.0 38.0 41.0 34.5 41.0 36-37 38.521972442094224 40.0 38.0 41.0 34.0 41.0 38-39 38.44071065582503 40.0 38.0 41.0 34.0 41.0 40-41 38.33198366283003 40.0 38.0 41.0 34.0 41.0 42-43 38.18203879801153 40.0 38.0 41.0 33.5 41.0 44-45 38.004863933013176 40.0 38.0 41.0 33.0 41.0 46-47 37.75346479995683 40.0 38.0 41.0 33.0 41.0 48-49 37.527456905318296 40.0 37.0 41.0 32.5 41.0 50-51 37.37496454607212 40.0 37.0 41.0 32.0 41.0 52-53 37.23836869041146 40.0 37.0 41.0 32.0 41.0 54-55 37.06303708377089 40.0 36.5 41.0 31.0 41.0 56-57 36.87900110547076 40.0 36.0 41.0 31.0 41.0 58-59 36.677761843687264 39.0 36.0 41.0 31.0 41.0 60-61 36.4377390978307 39.0 35.0 41.0 31.0 41.0 62-63 36.2046310785327 39.0 35.0 41.0 31.0 41.0 64-65 35.91968734132206 38.0 35.0 40.5 30.0 41.0 66-67 35.59113942352259 37.5 35.0 40.0 30.0 41.0 68-69 35.25733844423322 37.0 35.0 40.0 29.0 41.0 70-71 34.98985222111075 36.5 35.0 39.0 29.5 41.0 72-73 34.77653994568112 36.0 35.0 39.0 29.5 41.0 74-75 34.427548774228 36.0 34.0 39.0 29.0 40.5 76-77 34.08277644724663 35.0 34.0 37.0 29.0 39.5 78-79 33.75493657032883 35.0 34.0 37.0 29.0 39.0 80-81 33.44341933591507 35.0 34.0 36.5 29.0 39.0 82-83 33.15743446384668 35.0 34.0 36.0 29.0 37.5 84-85 32.91972296819593 35.0 34.0 36.0 29.0 37.0 86-87 32.720379194459085 35.0 34.0 35.0 29.0 37.0 88-89 32.55111868383951 35.0 34.0 35.0 28.0 36.0 90-91 32.407058133373184 35.0 34.0 35.0 27.5 36.0 92-93 32.27909366616443 35.0 33.5 35.0 27.0 36.0 94-95 32.19072207026035 35.0 33.0 35.0 27.0 36.0 96-97 32.111389323559806 35.0 33.0 35.0 27.0 35.0 98-99 32.045826536555936 35.0 33.0 35.0 27.0 35.0 100-101 31.985022185511554 35.0 33.0 35.0 27.0 35.0 102-103 31.01915549781465 34.0 31.5 35.0 24.5 35.0 104-105 31.733468438740452 35.0 33.0 35.0 25.0 35.0 106-107 32.015272860346805 35.0 33.5 35.0 27.0 35.0 108-109 32.029600743806114 35.0 34.0 35.0 27.0 35.0 110-111 31.997372604525097 35.0 34.0 35.0 27.0 35.0 112-113 31.93082540894809 35.0 34.0 35.0 27.0 35.0 114-115 31.859473081994388 35.0 34.0 35.0 27.0 35.0 116-117 31.799615783189374 35.0 34.0 35.0 26.5 35.0 118-119 31.6968056183926 35.0 33.5 35.0 25.0 35.0 120-121 31.562962025211377 35.0 33.0 35.0 25.0 35.0 122-123 31.421213762626785 35.0 33.0 35.0 25.0 35.0 124-125 30.73426675742872 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14984281786406228 1101 2 0.13237290643878907 1101 3 0.16766088323316097 1101 4 0.08726296790930377 1101 5 0.21975538040519638 1101 6 0.19862221467548125 1101 7 0.28355128479272196 1101 8 0.21087667455794445 1101 9 0.26615108944887567 1101 10-11 0.2291871846121012 1101 12-13 0.1673905092760819 1101 14-15 0.2794082551809467 1101 16-17 0.3008667117910022 1101 18-19 0.2866124339024836 1101 20-21 0.17814449353238615 1101 22-23 0.28817729008120097 1101 24-25 0.2770714811764279 1101 26-27 0.24537246924441547 1101 28-29 0.23793500263636247 1101 30-31 0.2208218552164496 1101 32-33 0.15646506149597883 1101 34-35 0.23146756685132885 1101 36-37 0.19055943461718527 1101 38-39 0.13537681280607217 1101 40-41 0.17311345432307235 1101 42-43 0.21227505398415758 1101 44-45 0.20881450787400269 1101 46-47 0.27927365395568415 1101 48-49 0.13088980742720224 1101 50-51 0.16195526617097045 1101 52-53 0.2800847962781816 1101 54-55 0.21845191325372326 1101 56-57 0.25506588790095464 1101 58-59 -5.893058587105315E-4 1101 60-61 0.23129995388419644 1101 62-63 0.04010424125729628 1101 64-65 -0.04692115299414468 1101 66-67 0.010299735622417927 1101 68-69 -0.0763396956148128 1101 70-71 -0.1523754025945223 1101 72-73 -0.11146553427361283 1101 74-75 -0.06792269641363191 1101 76-77 -0.12216808108979649 1101 78-79 -0.32305886332439826 1101 80-81 -0.23945589655397725 1101 82-83 -0.23808144049272784 1101 84-85 -0.3396705796966941 1101 86-87 -0.28280038248588824 1101 88-89 -0.36939251354952773 1101 90-91 -0.7650931199011488 1101 92-93 -0.6568737232108575 1101 94-95 -0.46917646537625046 1101 96-97 -0.24878254400613997 1101 98-99 -0.2515428073829824 1101 100-101 -0.4426367883428455 1101 102-103 -0.31123684468872526 1101 104-105 -0.16300596226319186 1101 106-107 -0.17490091330513735 1101 108-109 -0.13504675267375887 1101 110-111 -0.2213051102752246 1101 112-113 -0.21635860183689104 1101 114-115 -0.33173291304433405 1101 116-117 -0.24914013919525502 1101 118-119 -0.28024673694723745 1101 120-121 -0.16263974595254993 1101 122-123 -0.18016350152837646 1101 124-125 -0.31935553682120243 1102 1 0.177987648024569 1102 2 0.1820218514964651 1102 3 0.1713509594876257 1102 4 0.16092229352831566 1102 5 0.12453506010088944 1102 6 0.18697963304409626 1102 7 0.22070622889055613 1102 8 0.29637055109325416 1102 9 0.3257611074015614 1102 10-11 0.2833385203983738 1102 12-13 0.23571024478507496 1102 14-15 0.32454236705638806 1102 16-17 0.34773565720417565 1102 18-19 0.3104912799537871 1102 20-21 0.31904817116619455 1102 22-23 0.32325078943241436 1102 24-25 0.2785396907073405 1102 26-27 0.329145732189005 1102 28-29 0.4004405573031704 1102 30-31 0.4249634147891399 1102 32-33 0.3843112727688407 1102 34-35 0.40551083104017493 1102 36-37 0.46166627974418617 1102 38-39 0.43836122418914414 1102 40-41 0.41044259277202855 1102 42-43 0.42980113869948866 1102 44-45 0.4645833626350182 1102 46-47 0.5958061198722504 1102 48-49 0.5253618030190168 1102 50-51 0.5406804479196694 1102 52-53 0.48832525558247397 1102 54-55 0.534476016063941 1102 56-57 0.5881699245884207 1102 58-59 0.6166879297835948 1102 60-61 0.5489895679903825 1102 62-63 0.53375503490809 1102 64-65 0.46018704302782254 1102 66-67 0.4530146790092573 1102 68-69 0.4996925836649453 1102 70-71 0.42210867995227375 1102 72-73 0.44802716911606666 1102 74-75 0.5206480211604401 1102 76-77 0.5601031999496797 1102 78-79 0.5481244140178916 1102 80-81 0.475055559390853 1102 82-83 0.5348313379071712 1102 84-85 0.4574321060958013 1102 86-87 0.456923039544364 1102 88-89 0.3198181016409336 1102 90-91 0.40995442804673843 1102 92-93 0.39907999086155854 1102 94-95 0.4039549447092483 1102 96-97 0.40462757704115404 1102 98-99 0.3854610497776463 1102 100-101 0.3418350294996344 1102 102-103 0.2924496765789826 1102 104-105 0.2339011992576303 1102 106-107 0.25701398031188205 1102 108-109 0.2926288192347428 1102 110-111 0.3376454576339114 1102 112-113 0.34066694074295967 1102 114-115 0.23799459722069471 1102 116-117 0.27600250464839604 1102 118-119 0.20212052873469588 1102 120-121 0.263196120308848 1102 122-123 0.2484754717914477 1102 124-125 0.24037535068124072 1103 1 0.09394411794622215 1103 2 0.09334006262125882 1103 3 0.11520536427045869 1103 4 0.11213851766523675 1103 5 -0.07239165384710589 1103 6 0.06442722306422155 1103 7 -0.022394019238703322 1103 8 0.0652957801732228 1103 9 0.1669153969117474 1103 10-11 0.19569125470468407 1103 12-13 0.14512548031249395 1103 14-15 0.19670597606718587 1103 16-17 0.19745529067623124 1103 18-19 0.1845432112591041 1103 20-21 0.14369991100168988 1103 22-23 0.21576002101321023 1103 24-25 0.2503545511102132 1103 26-27 0.22667420844089037 1103 28-29 0.21444618417147154 1103 30-31 0.19985449190458837 1103 32-33 0.2148165186512685 1103 34-35 0.2274351027710111 1103 36-37 0.22793144591263825 1103 38-39 0.22671673285412197 1103 40-41 0.21539443224998678 1103 42-43 0.22250379387796215 1103 44-45 0.15688060976786034 1103 46-47 0.09812622615754663 1103 48-49 0.07480400062369341 1103 50-51 0.06231946868828686 1103 52-53 0.08698496681471823 1103 54-55 0.04448324502667589 1103 56-57 0.039947858668092806 1103 58-59 0.13192931852594825 1103 60-61 0.20396094520547337 1103 62-63 0.12526100368678073 1103 64-65 0.14914822154412377 1103 66-67 0.031550520988034236 1103 68-69 -0.03559354649545554 1103 70-71 0.04075549627800257 1103 72-73 0.03872329869033564 1103 74-75 0.10655447447501842 1103 76-77 0.10840732436599154 1103 78-79 0.11523556420447534 1103 80-81 0.05267215083723187 1103 82-83 0.06973355153279215 1103 84-85 0.158919904778962 1103 86-87 0.14836258001678004 1103 88-89 0.16852079589489222 1103 90-91 0.20221357659215045 1103 92-93 0.19220075361481292 1103 94-95 0.0950975115771513 1103 96-97 0.086739801803283 1103 98-99 0.10769792530273037 1103 100-101 0.10787551169099174 1103 102-103 0.10472523682359736 1103 104-105 0.12986567965151252 1103 106-107 0.05301460551947201 1103 108-109 0.020618581460475127 1103 110-111 0.005451174375799894 1103 112-113 -0.04684356079075158 1103 114-115 -0.016972872446974208 1103 116-117 -0.03665990279157327 1103 118-119 -0.09763524955106107 1103 120-121 -0.12616460493195447 1103 122-123 -0.23958892459016212 1103 124-125 -0.36379790997171213 1104 1 0.019048792126504566 1104 2 -0.015916470580194186 1104 3 -0.005707319883327955 1104 4 0.059094369695678495 1104 5 0.05282262073603761 1104 6 0.16012344320262173 1104 7 0.16400909130057784 1104 8 0.13111204559423584 1104 9 0.173159142540932 1104 10-11 0.13032607672316487 1104 12-13 0.18917428146954052 1104 14-15 0.16367678063202362 1104 16-17 0.20163968208489536 1104 18-19 0.21648831575297578 1104 20-21 0.09105404651724314 1104 22-23 0.10478156373869751 1104 24-25 0.17881356429446527 1104 26-27 0.1494007728224318 1104 28-29 0.20008511845887966 1104 30-31 0.22073362622883508 1104 32-33 0.20011802279122293 1104 34-35 0.1822023983199017 1104 36-37 0.3440747094803598 1104 38-39 0.3193760816654603 1104 40-41 0.1402669622162236 1104 42-43 0.21577257759683732 1104 44-45 0.2668516062299915 1104 46-47 0.2739250791526189 1104 48-49 0.325546153762879 1104 50-51 0.2425557857026206 1104 52-53 0.32459825383511287 1104 54-55 0.36546882439552775 1104 56-57 0.31496212217528097 1104 58-59 0.431878961584097 1104 60-61 0.41741320334725884 1104 62-63 0.4339732053927392 1104 64-65 0.26674906743771487 1104 66-67 0.2309685183787451 1104 68-69 0.31663362126785444 1104 70-71 0.3320755565002713 1104 72-73 0.491756757078889 1104 74-75 0.4853697211660801 1104 76-77 0.4199097642950491 1104 78-79 0.4571015657989008 1104 80-81 0.47229261919542864 1104 82-83 0.37952147282141624 1104 84-85 0.41645219568496117 1104 86-87 0.4471753281792701 1104 88-89 0.3431444389298548 1104 90-91 0.345178351202982 1104 92-93 0.3590264760952664 1104 94-95 0.30782564719831385 1104 96-97 0.21033615034544795 1104 98-99 0.23514059115544228 1104 100-101 0.1933564204856708 1104 102-103 0.21048189817122775 1104 104-105 0.32738692036413397 1104 106-107 0.27587047519416075 1104 108-109 0.2641774649530575 1104 110-111 0.2695862134056526 1104 112-113 0.2644412238141918 1104 114-115 0.11177733522711719 1104 116-117 0.10303939800679984 1104 118-119 0.1470782374804145 1104 120-121 0.14870734547869446 1104 122-123 0.05793173656686079 1104 124-125 -0.02482678858314813 1105 1 0.11141386690549382 1105 2 0.09204220246193273 1105 3 0.10266209704531093 1105 4 0.11256633546872052 1105 5 0.0904854114856235 1105 6 0.08622526048919354 1105 7 0.16251472340289297 1105 8 0.11687632580065355 1105 9 0.10750071179943177 1105 10-11 0.12848283164235852 1105 12-13 0.15026618413855175 1105 14-15 0.15693426666258858 1105 16-17 0.19596325259731628 1105 18-19 0.15985317559300682 1105 20-21 0.16312365357437386 1105 22-23 0.1064159289703781 1105 24-25 0.16014512709203643 1105 26-27 0.18502993487301467 1105 28-29 0.07189986021003136 1105 30-31 0.16019928925174298 1105 32-33 0.10135867342484772 1105 34-35 -0.03391553619961485 1105 36-37 -0.03815149687252273 1105 38-39 0.02804641313704792 1105 40-41 0.030078855547820638 1105 42-43 0.05880303833794187 1105 44-45 -0.032946784853031374 1105 46-47 -0.01868338051315277 1105 48-49 0.047226351379165976 1105 50-51 -0.07421736185202121 1105 52-53 -0.03804682198985887 1105 54-55 -0.1818824834474455 1105 56-57 -0.11504526993907405 1105 58-59 -0.1791612473085138 1105 60-61 -0.21899426734417915 1105 62-63 -0.06959497267111914 1105 64-65 -0.16483496891184046 1105 66-67 -0.13206880510183794 1105 68-69 -0.13967573010759082 1105 70-71 0.002471127099425985 1105 72-73 0.08849172586171505 1105 74-75 0.12575898391268936 1105 76-77 0.06416952281080768 1105 78-79 0.10249342120685156 1105 80-81 0.09604930574235482 1105 82-83 0.0919130546127036 1105 84-85 0.08246798636375274 1105 86-87 0.058002511515823585 1105 88-89 0.06248869014183356 1105 90-91 0.08119312777807863 1105 92-93 0.05341803620131458 1105 94-95 0.08543653369714832 1105 96-97 0.08911472303595502 1105 98-99 0.1039130218216826 1105 100-101 0.13147345315448433 1105 102-103 0.18619602807982716 1105 104-105 0.0921917181588725 1105 106-107 0.06325279292496333 1105 108-109 0.09742597074461656 1105 110-111 0.13003948586725045 1105 112-113 0.12162927371830179 1105 114-115 0.05521809339400008 1105 116-117 -0.027834829536388384 1105 118-119 -0.012442506042553703 1105 120-121 0.08840293578590419 1105 122-123 0.02337057240132978 1105 124-125 -0.12100974054333236 1106 1 0.028011554311923703 1106 2 0.05902732338001471 1106 3 0.07408424500837896 1106 4 0.020433675577351096 1106 5 0.04121633616537679 1106 6 0.036867406306242856 1106 7 0.07815436552827038 1106 8 0.05927928220064871 1106 9 0.11181510904843606 1106 10-11 0.0859453018152152 1106 12-13 0.10247154050584584 1106 14-15 0.10888574103040582 1106 16-17 0.09565846645856624 1106 18-19 0.06660630903976994 1106 20-21 0.031141100217638495 1106 22-23 -0.01743505538921397 1106 24-25 0.061562906368621384 1106 26-27 0.03549982414853048 1106 28-29 4.568268598390546E-4 1106 30-31 0.046017217216956396 1106 32-33 0.03011970371951378 1106 34-35 0.029832158104738937 1106 36-37 0.03866776724651544 1106 38-39 0.04752762523039422 1106 40-41 -0.022424899989680114 1106 42-43 -0.012067403282614464 1106 44-45 -0.06626504494061436 1106 46-47 -0.06000642827235936 1106 48-49 -0.037889584873781246 1106 50-51 -0.08913700101384592 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-0.5701688942403962 2216 32-33 -0.5669425065525147 2216 34-35 -0.6040904247997503 2216 36-37 -0.5755696360785691 2216 38-39 -0.5028245741796198 2216 40-41 -0.8126807657755108 2216 42-43 -0.6971319818304238 2216 44-45 -0.7004873720238365 2216 46-47 -0.7033049200546131 2216 48-49 -0.7365227810964043 2216 50-51 -0.5978588888679752 2216 52-53 -0.6596084344213864 2216 54-55 -0.6583744633057407 2216 56-57 -0.5739424491063758 2216 58-59 -0.528341630410182 2216 60-61 -0.6338751669607063 2216 62-63 -0.5625823484340557 2216 64-65 -0.6172348135321784 2216 66-67 -0.5756568356790552 2216 68-69 -0.666863747424209 2216 70-71 -0.7715665063443851 2216 72-73 -0.71921936814514 2216 74-75 -0.7383944383675711 2216 76-77 -0.7548956011577914 2216 78-79 -0.6639303566377137 2216 80-81 -0.5897004659807124 2216 82-83 -0.610679933555808 2216 84-85 -0.5564647441887516 2216 86-87 -0.5841542028529716 2216 88-89 -0.5253504551547223 2216 90-91 -0.4962694164269337 2216 92-93 -0.5550331471272258 2216 94-95 -0.6099831175057808 2216 96-97 -0.6730525097286879 2216 98-99 -0.6856810452120996 2216 100-101 -0.6839121240672625 2216 102-103 -0.7641831963046926 2216 104-105 -0.7595933101892669 2216 106-107 -0.6687466607958932 2216 108-109 -0.5173651675699524 2216 110-111 -0.5532799562649977 2216 112-113 -0.45909112649243156 2216 114-115 -0.5907543771642239 2216 116-117 -0.5560340578270164 2216 118-119 -0.4995154912418478 2216 120-121 -0.39429396072879186 2216 122-123 -0.365998532846767 2216 124-125 -0.3321427544691282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 265.0 3 16.0 4 30.0 5 96.0 6 220.0 7 418.0 8 829.0 9 1491.0 10 2566.0 11 3508.0 12 4660.0 13 6623.0 14 10636.0 15 9054.0 16 4141.0 17 3665.0 18 3689.0 19 3489.0 20 3405.0 21 3360.0 22 3571.0 23 3909.0 24 4955.0 25 6680.0 26 8622.0 27 11526.0 28 14716.0 29 18995.0 30 23950.0 31 30445.0 32 38975.0 33 50572.0 34 69825.0 35 105866.0 36 213550.0 37 499706.0 38 276109.0 39 1405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.899102375673635 19.111468167966187 13.314414855779477 23.675014600580703 2 15.658253190161725 20.754763970231153 39.267248595332674 24.31973424427445 3 18.36437367955737 25.946187509425556 30.09391659022454 25.59552222079254 4 12.260832990900274 17.183360105372532 37.9245651100144 32.63124179371279 5 12.604281746921734 39.15165258707336 34.662865038030134 13.581200627974782 6 31.02436418762632 37.808046485206646 17.516434565830632 13.651154761336409 7 27.746624440173832 33.387984266065644 21.40594021049602 17.4594510832645 8 26.49325026391558 35.32387249591501 19.89612172077109 18.286755519398316 9 27.03865273690488 15.158785172025919 19.963985727113364 37.838576363955845 10-11 24.522392354957116 27.05695042261082 27.894942920905574 20.52571430152649 12-13 25.566190580946333 24.563069251724965 28.236061590909404 21.634678576419297 14-15 22.48221769334324 25.633812462903084 26.303528513259423 25.580441330494253 16-17 21.73249682817055 29.197018687851862 26.95086535255386 22.119619131423732 18-19 21.796175541563073 27.30889814034636 29.459931181331793 21.434995136758772 20-21 22.59411490636502 26.559398312111064 29.560193520247026 21.286293261276892 22-23 22.275478057304614 26.676918904933665 29.308084602411004 21.739518435350714 24-25 22.007653897718356 26.820879146725996 29.448101675639105 21.723365279916543 26-27 21.780908343779316 27.0871208662278 29.335773957269712 21.796196832723176 28-29 21.772418893238687 26.904435941621536 29.499049831446257 21.824095333693524 30-31 21.76386475328183 26.656824873265005 29.613928674208047 21.965381699245125 32-33 21.6080673161833 26.85096502232908 29.43277461343937 22.10819304804825 34-35 22.280140072803544 26.743888417705847 29.085445240305656 21.890526269184953 36-37 22.36734696701159 26.381112084220547 29.106706292051815 22.144834656716046 38-39 21.946811498556247 26.738591444846136 29.16353634425384 22.151060712343778 40-41 22.188897405529854 26.43434684435393 29.279519845925662 22.09723590419056 42-43 22.215811690872187 26.32922828732278 29.086921270834804 22.368038750970225 44-45 21.98196933042876 26.683613079429996 29.046022343253753 22.28839524688749 46-47 22.486195451105402 27.0117423409139 28.155676294915803 22.346385913064893 48-49 22.377912637730386 27.019731483034796 28.067649810709895 22.53470606852492 50-51 22.446071981504463 27.05062059938929 28.00600883546472 22.497298583641523 52-53 22.639079705964146 27.019144429271318 27.892279552664824 22.44949631209972 54-55 22.24794505575087 27.080920736777585 27.99141219393748 22.67972201353406 56-57 22.317346425584404 27.226041256640265 27.97676572789213 22.479846589883206 58-59 22.32897612754849 26.786179387376247 28.06636152044587 22.818482964629393 60-61 22.13657549527425 27.02897711059035 28.35191062148163 22.482536772653773 62-63 22.10000705619638 26.986872707600906 28.359095367952968 22.554024868249744 64-65 21.895654074815052 26.816382550994856 28.316377708507147 22.97158566568295 66-67 21.884793066664454 27.000189548804666 28.19182200675458 22.923195377776302 68-69 21.97217298063869 26.750049721989228 28.399367017458903 22.87841027991318 70-71 21.98445215015176 26.87550478628192 28.11998996912913 23.02005309443719 72-73 22.061608981028492 26.895456079959267 27.86381660505169 23.179118333960552 74-75 21.956323527987504 27.064560046155826 27.77488381488415 23.204232610972525 76-77 22.08092909417054 26.897132345972462 27.933356254457685 23.088582305399306 78-79 22.19523879525782 26.87962090225954 27.788037321757475 23.137102980725164 80-81 22.090924970555832 27.263690024264413 27.576066259319663 23.06931874586009 82-83 22.08641350140916 27.06258846187371 27.640608548512734 23.21038948820439 84-85 22.037296840345945 26.86138309750418 27.658456574645562 23.442863487504304 86-87 22.129615409626034 26.79185880966118 27.710374960741262 23.368150819971525 88-89 22.033492028573445 26.896629490196727 27.588793930010837 23.48108455121899 90-91 21.98479804225072 27.004868431292856 27.566493432373772 23.443840094082653 92-93 21.960889302114506 26.61988131754406 27.70864550084467 23.71058387949677 94-95 21.795518346802368 26.484706732026414 27.767377958932936 23.952396962238282 96-97 21.93051998633035 26.44299215932061 27.717465746317288 23.90902210803175 98-99 21.913912901511964 26.360913875545126 27.561056936665707 24.164116286277203 100-101 21.688533957599176 26.28772124980457 27.78619448260786 24.237550309988393 102-103 21.699229525349566 26.11329695701426 27.825324490025338 24.362149027610837 104-105 21.62505067387608 26.184696420245203 27.77018342213656 24.42006948374216 106-107 21.64361756219315 26.177572763616563 27.71146639622507 24.467343277965213 108-109 21.723213195523687 26.11699384408592 27.52032918246403 24.63946377792636 110-111 21.53388442787794 26.290827935498555 27.57572361425596 24.59956402236755 112-113 21.463634992646334 26.315219662160388 27.773534836164803 24.447610509028472 114-115 21.334997765537814 26.40010155388513 27.48557976338222 24.779320917194845 116-117 21.209999740580926 26.37475679461792 27.60540629350674 24.809837171294415 118-119 21.095156267078416 26.468103917019132 27.38098202883632 25.05575778706613 120-121 21.021790608433854 26.575908783692636 27.513799427338466 24.88850118053504 122-123 21.006387289894963 26.662628923569052 27.378492411418122 24.952491375117862 124-125 20.944762434470764 26.536521350528314 27.421382212020717 25.097334002980205 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 76.0 1 71.5 2 122.5 3 182.0 4 131.5 5 109.0 6 257.0 7 640.5 8 1115.5 9 1358.0 10 1100.0 11 769.0 12 760.5 13 870.0 14 985.5 15 1087.5 16 1197.0 17 1311.0 18 1476.5 19 1622.5 20 1793.5 21 2114.5 22 2504.5 23 3041.0 24 3861.5 25 5141.0 26 6733.5 27 8808.0 28 10808.5 29 13353.0 30 17292.0 31 20591.0 32 24120.5 33 27158.5 34 29650.5 35 32734.5 36 36534.5 37 40362.0 38 42463.0 39 44862.5 40 46502.5 41 47905.5 42 50059.5 43 52423.5 44 54582.5 45 56327.0 46 56279.0 47 55214.5 48 54832.5 49 53807.0 50 52345.5 51 50119.0 52 48592.0 53 47956.5 54 47472.5 55 44759.0 56 39498.5 57 35069.5 58 32334.0 59 28635.0 60 23530.5 61 19077.0 62 15619.5 63 12008.5 64 8744.5 65 6579.0 66 4728.5 67 3676.0 68 2587.5 69 1518.0 70 1055.5 71 750.0 72 540.0 73 397.0 74 316.0 75 279.0 76 167.0 77 60.5 78 26.0 79 18.0 80 7.5 81 2.0 82 2.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02697957438683729 2 0.0 3 0.0 4 0.0 5 0.061845485902134706 6 0.018055561320421878 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.113027813865841E-4 22-23 0.0 24-25 0.0 26-27 4.150703751821121E-4 28-29 3.458919793184268E-4 30-31 1.383567917273707E-4 32-33 1.0376759379552803E-4 34-35 2.767135834547414E-4 36-37 0.0 38-39 0.0 40-41 1.383567917273707E-4 42-43 0.0 44-45 6.917839586368535E-5 46-47 0.0 48-49 3.804811772502694E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.917839586368535E-5 58-59 1.0376759379552803E-4 60-61 3.458919793184267E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.458919793184267E-5 70-71 3.458919793184267E-5 72-73 1.383567917273707E-4 74-75 0.0 76-77 6.917839586368535E-5 78-79 3.458919793184267E-5 80-81 3.804811772502694E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.458919793184267E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.383567917273707E-4 100-101 0.0 102-103 3.458919793184267E-5 104-105 0.0013835679172737071 106-107 6.917839586368535E-5 108-109 0.0012106219276144937 110-111 0.003528098189047953 112-113 0.0 114-115 0.0 116-117 3.458919793184267E-5 118-119 0.0 120-121 4.8424877104579747E-4 122-123 7.609623545005389E-4 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1445538.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.64529764827272 #Duplication Level Percentage of deduplicated Percentage of total 1 82.94876430515255 53.622475580630045 2 10.30253518266958 13.320209068309532 3 3.0688242600966 5.95155173172555 4 1.2505084782734035 3.23357971158691 5 0.6558556183345386 2.11989908307641 6 0.3746423316923049 1.4531319026335179 7 0.23110337241063697 1.045782240790367 8 0.17714226685254136 0.9161131653417831 9 0.12597071910041716 0.7329073168092077 >10 0.7556453413043525 9.436400263460582 >50 0.07624176172573081 3.3185379706736025 >100 0.03169556623146602 3.4383920170384883 >500 4.2831846237224305E-4 0.2008820589126466 >1k 6.424776935583647E-4 1.210137889011333 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3500 0.24212438552289875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3327 0.23015652303848116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1914 0.13240744968309376 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 6.917839586368536E-5 8 0.0 0.0 0.0 0.0 6.917839586368536E-5 9 0.0 0.0 0.0 0.0 6.917839586368536E-5 10-11 0.0 0.0 0.0 0.0 6.917839586368536E-5 12-13 0.0 0.0 0.0 6.917839586368536E-5 6.917839586368536E-5 14-15 0.0 0.0 0.0 6.917839586368536E-5 6.917839586368536E-5 16-17 0.0 0.0 0.0 6.917839586368536E-5 6.917839586368536E-5 18-19 0.0 0.0 0.0 6.917839586368536E-5 6.917839586368536E-5 20-21 0.0 0.0 0.0 6.917839586368536E-5 6.917839586368536E-5 22-23 0.0 0.0 0.0 1.0376759379552804E-4 6.917839586368536E-5 24-25 0.0 0.0 0.0 2.4212438552289874E-4 1.0376759379552804E-4 26-27 0.0 0.0 0.0 8.993191462279095E-4 1.3835679172737072E-4 28-29 0.0 0.0 0.0 0.0037010441787071664 1.3835679172737072E-4 30-31 0.0 0.0 0.0 0.012244576067872309 1.3835679172737072E-4 32-33 0.0 0.0 0.0 0.03223713247247738 1.3835679172737072E-4 34-35 0.0 0.0 0.0 0.05970095563036046 1.3835679172737072E-4 36-37 0.0 0.0 0.0 0.10947481145428209 1.3835679172737072E-4 38-39 0.0 0.0 0.0 0.18826900434301969 1.3835679172737072E-4 40-41 0.0 0.0 0.0 0.2771632430278554 1.3835679172737072E-4 42-43 0.0 0.0 0.0 0.3630828106905526 1.3835679172737072E-4 44-45 0.0 0.0 0.0 0.4682339724033543 1.3835679172737072E-4 46-47 0.0 0.0 0.0 0.5983239458250147 1.3835679172737072E-4 48-49 0.0 0.0 0.0 0.7395170517827965 1.3835679172737072E-4 50-51 0.0 0.0 0.0 0.8922975390477454 1.3835679172737072E-4 52-53 0.0 0.0 0.0 1.0361194240483473 1.3835679172737072E-4 54-55 0.0 0.0 0.0 1.191459511960253 1.3835679172737072E-4 56-57 0.0 0.0 0.0 1.3738137634569276 1.3835679172737072E-4 58-59 0.0 0.0 0.0 1.581694843027302 1.3835679172737072E-4 60-61 0.0 0.0 0.0 1.8081849110850077 1.3835679172737072E-4 62-63 0.0 0.0 0.0 2.0349171035282367 1.3835679172737072E-4 64-65 0.0 0.0 0.0 2.2599198360748733 1.3835679172737072E-4 66-67 0.0 0.0 0.0 2.526256660150062 1.3835679172737072E-4 68-69 0.0 0.0 0.0 2.826110417021206 1.3835679172737072E-4 70-71 0.0 0.0 0.0 3.1522173751226186 1.3835679172737072E-4 72-73 0.0 0.0 0.0 3.487213757092515 2.0753518759105606E-4 74-75 0.0 0.0 0.0 3.8125251636414954 2.0753518759105606E-4 76-77 0.0 0.0 0.0 4.161668527565515 2.0753518759105606E-4 78-79 0.0 0.0 0.0 4.55425592409193 2.0753518759105606E-4 80-81 0.0 0.0 0.0 4.979841415445322 2.0753518759105606E-4 82-83 0.0 0.0 0.0 5.4383904124277604 2.0753518759105606E-4 84-85 0.0 0.0 0.0 5.882446535476757 2.7671358345474143E-4 86-87 0.0 0.0 0.0 6.329892399923073 2.7671358345474143E-4 88-89 0.0 0.0 0.0 6.82012510221108 2.7671358345474143E-4 90-91 0.0 0.0 0.0 7.35978576834369 2.7671358345474143E-4 92-93 0.0 0.0 0.0 7.926183884477613 2.7671358345474143E-4 94-95 0.0 0.0 0.0 8.493066249382583 2.7671358345474143E-4 96-97 0.0 0.0 0.0 9.045351972760315 2.7671358345474143E-4 98-99 0.0 0.0 0.0 9.622541918648974 3.458919793184268E-4 100-101 0.0 0.0 0.0 10.26724997890059 3.458919793184268E-4 102-103 0.0 0.0 0.0 10.940978376217021 3.458919793184268E-4 104-105 0.0 0.0 0.0 11.613669097595498 3.458919793184268E-4 106-107 0.0 0.0 0.0 12.26003743934784 3.458919793184268E-4 108-109 0.0 0.0 0.0 12.931655895590431 3.458919793184268E-4 110-111 0.0 0.0 0.0 13.64945093107203 3.458919793184268E-4 112-113 0.0 0.0 0.0 14.416673930398233 3.458919793184268E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2445 0.0 74.0633 1 GTATCAA 4680 0.0 56.003498 1 ATCAACG 5880 0.0 44.35624 3 TCAACGC 5925 0.0 44.01936 4 CAACGCA 6075 0.0 42.834442 5 TATCAAC 6140 0.0 42.477962 2 AACGCAG 6300 0.0 41.371944 6 GTACATG 7460 0.0 35.373108 1 ACGCAGA 7505 0.0 34.80255 7 CGCAGAG 7590 0.0 34.256023 8 TGGTATC 1170 0.0 32.57257 2 ACATGGG 7715 0.0 32.571255 3 TACATGG 7980 0.0 32.310448 2 CATGGGA 5615 0.0 30.436176 4 GCAGAGT 8620 0.0 29.74865 9 ATAGCGC 85 0.007212572 27.998728 8 GTGGTAT 1455 0.0 26.610846 1 GAGTACT 4835 0.0 26.087751 12-13 CAGAGTA 8430 0.0 25.231653 10-11 AGAGTAC 8450 0.0 24.925499 10-11 >>END_MODULE