FastQCFastQC Report
Thu 26 May 2016
SRR1364339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032708
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27150.046.9337651
GGTATCA17950.046.7730981
ATCAACG29200.042.3985443
CAACGCA31850.039.0577555
TCAACGC31800.038.9319954
AACGCAG32800.037.923716
GTACATG76100.037.7923551
TACATGG78800.035.7277262
TATCAAC35850.034.533822
ACATGGG78900.034.0227933
ACGCAGA38950.031.9247557
CGCAGAG40850.030.292758
CATGGGG48150.028.6788774
AGCGTAC1055.9111696E-428.3301018
GTATAGG6450.026.7719361
CATGGGA39850.025.3916574
GCAGAGT53300.022.1006689
GAGTACA56800.021.805021
GTAAGGT7550.021.2856674
ATGGGAT21250.020.4472265
Produced by FastQC (version 0.11.5)