FastQCFastQC Report
Thu 26 May 2016
SRR1364339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032708
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25980.12780979855444066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.12397255287035817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29950.062.6380161
GTATCAA46900.051.174841
ATCAACG57650.041.313193
TCAACGC59600.040.0614054
CAACGCA60100.039.827195
AACGCAG60600.039.674586
TATCAAC62150.038.3218882
GTACATG75100.035.6066171
TACATGG78450.033.6990852
ACATGGG76750.033.4369743
ACGCAGA74600.032.380537
CGCAGAG76250.031.6018098
CATGGGG46400.027.07654
GTGGTAT17650.026.9941431
TGGTATC15850.026.6720942
CATGGGA39750.026.3635314
GCAGAGT90200.026.3186199
GAGTACT49450.024.48470512-13
CAGAGTA85300.024.20344510-11
GGACCGA5100.023.337996
Produced by FastQC (version 0.11.5)