FastQCFastQC Report
Thu 26 May 2016
SRR1364338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803275
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.062.1577071
GTATCAA35450.049.709021
ATCAACG40050.042.352753
TCAACGC41300.041.2149934
CAACGCA42300.040.2395255
AACGCAG43700.038.9503866
GTACATG106800.037.960821
TATCAAC48050.036.168352
TACATGG112700.035.699492
ACATGGG109350.035.5413253
CATGGGG42900.031.2150824
CATGGGA74500.030.1178764
ACGCAGA56650.030.0364657
CCGCGTA800.005365801529.7468269
CGCAGAG57000.029.642458
ATGGGAT32700.025.6624765
GCAGAGT65850.024.6647939
ATGGGAG25000.024.0441025
GTGGTAT11400.023.4999751
ATGGGGA22900.022.8704325
Produced by FastQC (version 0.11.5)