FastQCFastQC Report
Thu 26 May 2016
SRR1364338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803275
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37970.21056133978455865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32930.18261219170675577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18790.104199304044031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31550.075.124421
GTATCAA60150.059.3046341
ATCAACG76300.046.5010223
TCAACGC77850.045.5751844
CAACGCA78600.044.837355
TATCAAC80900.043.9305422
AACGCAG80650.043.6794786
CGCGAGT600.001308963439.6651048
GTGGTAT13250.037.7538151
ACGCAGA96750.036.4672977
CGCAGAG98200.035.807658
GTACATG97600.033.6811331
ACATGGG101050.031.7536183
TACATGG105550.030.9638442
GCAGAGT110500.030.9064779
ACGCGAG850.007212825627.9988987
CATGGGA68650.027.9226154
TGGTATC16850.027.5572222
GAGTACT57400.026.27640312-13
CAGAGTA109750.025.6422710-11
Produced by FastQC (version 0.11.5)