FastQCFastQC Report
Thu 26 May 2016
SRR1364337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986187
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.051.4180561
GTATCAA25450.043.973251
GTACATG75050.040.6104471
CAACGCA27150.039.6750835
ATCAACG27150.039.6750833
TCAACGC27550.039.099044
ACATGGG78400.037.27133
AACGCAG28950.037.2063646
TACATGG81350.036.9439162
TATCAAC33500.032.5098842
CATGGGG49750.031.939444
ACGCAGA35950.030.1204417
CGCAGAG36800.029.1013768
CATGGGA39950.028.1549114
ATGGGGA28150.027.2722155
GAGTACA57400.026.652611
GTATAGG7900.026.3729951
ATGGGAT22250.022.735165
AGTACAT62500.022.6624092
GCAGAGT46050.022.222239
Produced by FastQC (version 0.11.5)