##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364337_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1986187 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.144058943090453 33.0 31.0 34.0 28.0 34.0 2 31.14327301507864 33.0 31.0 34.0 28.0 34.0 3 31.219131934707054 33.0 31.0 34.0 28.0 34.0 4 34.64801602266051 37.0 35.0 37.0 32.0 37.0 5 34.69940292631056 37.0 35.0 37.0 32.0 37.0 6 34.76810239922021 37.0 35.0 37.0 32.0 37.0 7 34.669735024949816 37.0 35.0 37.0 32.0 37.0 8 34.61574312992684 37.0 35.0 37.0 32.0 37.0 9 36.16816493109662 39.0 37.0 39.0 32.0 39.0 10-11 36.07614187385175 39.0 37.0 39.0 31.5 39.0 12-13 36.11770946038817 39.0 37.0 39.0 32.0 39.0 14-15 37.45075086082025 40.0 38.0 41.0 32.0 41.0 16-17 37.477434148949726 40.0 38.0 41.0 32.0 41.0 18-19 37.47948456011443 40.0 38.0 41.0 32.0 41.0 20-21 37.48392925741635 40.0 38.0 41.0 31.5 41.0 22-23 37.454099488114664 40.0 38.0 41.0 31.0 41.0 24-25 37.45967499535543 40.0 38.0 41.0 31.0 41.0 26-27 37.413758875674844 40.0 38.0 41.0 31.0 41.0 28-29 37.4145259232892 40.0 38.0 41.0 31.0 41.0 30-31 37.32341466337259 40.0 38.0 41.0 30.5 41.0 32-33 37.27947494369866 40.0 38.0 41.0 30.0 41.0 34-35 37.27483993199029 40.0 38.0 41.0 30.0 41.0 36-37 37.23263544671272 40.0 37.0 41.0 30.0 41.0 38-39 37.145206619517694 40.0 37.0 41.0 30.0 41.0 40-41 37.083844572540244 40.0 37.0 41.0 30.0 41.0 42-43 36.95821214215982 40.0 37.0 41.0 30.0 41.0 44-45 36.857171807085635 40.0 37.0 41.0 30.0 41.0 46-47 36.74813977737242 40.0 36.5 41.0 30.0 41.0 48-49 36.60987787151965 40.0 36.0 41.0 30.0 41.0 50-51 36.4927544586688 40.0 36.0 41.0 30.0 41.0 52-53 36.3364766258162 40.0 35.5 41.0 29.5 41.0 54-55 36.1558956936079 39.0 35.0 41.0 29.0 41.0 56-57 35.96302840568386 39.0 35.0 41.0 29.0 41.0 58-59 35.73528046452826 39.0 35.0 41.0 28.5 41.0 60-61 35.487126086315136 38.5 35.0 40.5 28.0 41.0 62-63 35.21760262251239 38.0 34.5 40.0 28.0 41.0 64-65 34.87559001242079 37.5 34.0 40.0 28.0 41.0 66-67 34.558396062405 37.0 34.0 40.0 27.5 41.0 68-69 34.227090148107905 36.5 34.0 39.0 27.0 41.0 70-71 33.86606749515529 36.0 34.0 39.0 26.0 41.0 72-73 33.50189156408737 35.5 33.0 38.5 26.0 40.0 74-75 33.1424103067838 35.0 33.0 37.5 26.0 39.5 76-77 32.90811061596919 35.0 33.0 37.0 26.0 39.0 78-79 32.74445885508263 35.0 33.0 37.0 26.0 39.0 80-81 32.48226904113258 35.0 33.0 36.0 26.0 38.5 82-83 32.199037401815644 35.0 33.0 36.0 26.0 37.0 84-85 31.958509193746607 35.0 33.0 35.5 25.0 37.0 86-87 31.757708866285 35.0 33.0 35.0 25.0 36.5 88-89 31.5065462114091 35.0 33.0 35.0 24.0 36.0 90-91 31.39132820827042 35.0 33.0 35.0 24.0 36.0 92-93 31.25546839245247 35.0 32.0 35.0 24.0 36.0 94-95 31.158546501412 35.0 32.0 35.0 24.0 35.0 96-97 31.059435742958748 35.0 32.0 35.0 23.0 35.0 98-99 30.929199264721802 35.0 32.0 35.0 22.5 35.0 100-101 30.849370930330327 35.0 32.0 35.0 20.0 35.0 102-103 29.8704968364006 33.5 30.0 35.0 19.0 35.0 104-105 30.553099229830828 34.5 31.5 35.0 19.0 35.0 106-107 30.87049029119615 35.0 32.5 35.0 20.0 35.0 108-109 30.90241779852552 35.0 33.0 35.0 20.0 35.0 110-111 30.833125732874095 35.0 33.0 35.0 19.5 35.0 112-113 30.743404321949544 35.0 33.0 35.0 19.0 35.0 114-115 30.64531285322077 35.0 32.5 35.0 18.0 35.0 116-117 30.51775311186711 35.0 32.0 35.0 13.0 35.0 118-119 30.34558150868977 35.0 32.0 35.0 4.5 35.0 120-121 30.16094859144683 35.0 32.0 35.0 2.0 35.0 122-123 29.974962579052224 35.0 32.0 35.0 2.0 35.0 124-125 29.186376962491448 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05780945860714226 1101 2 0.11811472450688143 1101 3 0.1372592394702501 1101 4 0.27546345191590405 1101 5 0.157916426785782 1101 6 0.11259650336803162 1101 7 0.10154786902796076 1101 8 0.03665787655912567 1101 9 0.1326735136663686 1101 10-11 0.3265577549241314 1101 12-13 0.34791594032693496 1101 14-15 0.43108344623212247 1101 16-17 0.2913341733682273 1101 18-19 0.21192455363885898 1101 20-21 0.2937452139984984 1101 22-23 0.37222658505013584 1101 24-25 0.4072287391575955 1101 26-27 0.46293990740407764 1101 28-29 0.37074801671280966 1101 30-31 0.4132816787685769 1101 32-33 0.2844682997445247 1101 34-35 0.3801229105643884 1101 36-37 0.31657400259605595 1101 38-39 0.400429960999638 1101 40-41 0.4143798538145944 1101 42-43 0.42073643395504234 1101 44-45 0.3359614327768412 1101 46-47 0.32563815388118655 1101 48-49 0.2573297899137543 1101 50-51 0.2811580846540238 1101 52-53 0.13540710739817996 1101 54-55 0.182692309308635 1101 56-57 0.1531514523643409 1101 58-59 0.21832819239349988 1101 60-61 0.07865978927283379 1101 62-63 0.12850747909095617 1101 64-65 0.13289508447385145 1101 66-67 0.1206392480558307 1101 68-69 0.017681949947089493 1101 70-71 0.16432385707625485 1101 72-73 0.07489749222049369 1101 74-75 0.08715273129300272 1101 76-77 0.06544574466857256 1101 78-79 0.200167833475156 1101 80-81 0.24019963406399825 1101 82-83 0.2310863271979926 1101 84-85 0.21682288528214144 1101 86-87 0.20290324787800884 1101 88-89 0.2766171021874264 1101 90-91 0.20395125209190823 1101 92-93 0.12070775627611141 1101 94-95 0.16214942420698364 1101 96-97 0.06489755060773206 1101 98-99 0.09474515669731431 1101 100-101 0.07125319977808076 1101 102-103 -0.21745832955984667 1101 104-105 0.04593173362790992 1101 106-107 0.176475788420003 1101 108-109 0.18465150846377654 1101 110-111 0.18428006100030458 1101 112-113 0.12672003782089902 1101 114-115 0.11679944432308531 1101 116-117 0.0643219428777364 1101 118-119 0.12157076048196558 1101 120-121 -0.01694865595194628 1101 122-123 -0.11003402443339638 1101 124-125 -0.10511434789987817 1102 1 0.13752875930131125 1102 2 0.1839688079337698 1102 3 0.2154685086037702 1102 4 0.2234346001001981 1102 5 0.22717349329542458 1102 6 0.26733233316462446 1102 7 0.18753498498737997 1102 8 0.2555571860552348 1102 9 0.28673633648050156 1102 10-11 0.26006434669962175 1102 12-13 0.276206887166893 1102 14-15 0.3596441052364838 1102 16-17 0.34721170825507386 1102 18-19 0.34566224722546224 1102 20-21 0.39617407518664294 1102 22-23 0.3654902117542349 1102 24-25 0.29495844019731976 1102 26-27 0.293729270098936 1102 28-29 0.247826315758644 1102 30-31 0.4624250051880381 1102 32-33 0.4191436824352692 1102 34-35 0.31094708879548705 1102 36-37 0.22214755735653569 1102 38-39 0.19309733025927756 1102 40-41 0.220329464756702 1102 42-43 0.15458585816159598 1102 44-45 0.11686461451444785 1102 46-47 0.1742752542538426 1102 48-49 0.24301396995821278 1102 50-51 0.25350380114605287 1102 52-53 0.20803589431996983 1102 54-55 0.15194894285257732 1102 56-57 0.11463456873941169 1102 58-59 0.06225106031756411 1102 60-61 0.1412239700711595 1102 62-63 0.14189248645646302 1102 64-65 0.13671715288855069 1102 66-67 0.15297637399893205 1102 68-69 0.1533816148825693 1102 70-71 0.14037429554317526 1102 72-73 0.0834197853698484 1102 74-75 0.0679249791083052 1102 76-77 0.08646049912099585 1102 78-79 0.053907562000567566 1102 80-81 0.1299807648074136 1102 82-83 0.1282236089068718 1102 84-85 0.10616130859423833 1102 86-87 0.05421308373816558 1102 88-89 0.08719995552309356 1102 90-91 0.05272301753248243 1102 92-93 0.1337416157222009 1102 94-95 0.04288335455757064 1102 96-97 -0.027004067869007997 1102 98-99 0.04334773442230144 1102 100-101 -0.018920057815119407 1102 102-103 -0.13683820551772286 1102 104-105 -0.066521302327601 1102 106-107 -0.020793445954009115 1102 108-109 0.10988157879778271 1102 110-111 0.06992522319434968 1102 112-113 0.02164053513039832 1102 114-115 -0.036581266494536635 1102 116-117 -6.355195153702198E-4 1102 118-119 -0.08208102506859305 1102 120-121 -0.2392845670442192 1102 122-123 -0.3073778817197521 1102 124-125 -0.37625198820623496 1103 1 0.24129242810986895 1103 2 0.25777053692246454 1103 3 0.2525658714434975 1103 4 0.24088704700876207 1103 5 0.08599004696529988 1103 6 0.16002013541302063 1103 7 0.21387289524837882 1103 8 0.23947137407193253 1103 9 0.2707540611723047 1103 10-11 0.4622752850911169 1103 12-13 0.33873369357446137 1103 14-15 0.5160774463084294 1103 16-17 0.2944143981428198 1103 18-19 0.353111600330287 1103 20-21 0.4529566865340655 1103 22-23 0.5283382769710272 1103 24-25 0.5678609494738183 1103 26-27 0.46783024713100474 1103 28-29 0.3910663545959707 1103 30-31 0.5411518582240049 1103 32-33 0.5123028347822327 1103 34-35 0.5520003887872207 1103 36-37 0.4701814672161788 1103 38-39 0.4403667656425583 1103 40-41 0.4504823940822291 1103 42-43 0.44119886118752305 1103 44-45 0.42395365507131544 1103 46-47 0.40034254276358183 1103 48-49 0.40315385021963834 1103 50-51 0.40856780205893983 1103 52-53 0.47303358749334024 1103 54-55 0.4003863014426301 1103 56-57 0.2271127793108647 1103 58-59 0.3897016578299315 1103 60-61 0.3897412560232283 1103 62-63 0.33667669733485184 1103 64-65 0.3971755867254103 1103 66-67 0.2989897693329482 1103 68-69 0.215101154933798 1103 70-71 0.29582182482855757 1103 72-73 0.26758718326940567 1103 74-75 0.23478576679369212 1103 76-77 0.2632064570098507 1103 78-79 0.2800540400812892 1103 80-81 0.293300278494911 1103 82-83 0.2540568209145633 1103 84-85 0.18849313158735015 1103 86-87 0.13495804118237587 1103 88-89 0.3280174083143059 1103 90-91 0.24811061851147898 1103 92-93 0.17675569375884947 1103 94-95 0.058016555350842935 1103 96-97 0.17414199213013148 1103 98-99 0.11476061900816958 1103 100-101 0.09108606093185401 1103 102-103 -0.13372448142402504 1103 104-105 0.0660098226168202 1103 106-107 0.12092022619919973 1103 108-109 0.13155554303754613 1103 110-111 0.19616803219107126 1103 112-113 0.10044694663285725 1103 114-115 -0.11309253305548594 1103 116-117 -0.05387673389974168 1103 118-119 -0.08756035314754484 1103 120-121 -0.30282534426280705 1103 122-123 -0.2701398467228806 1103 124-125 -0.3922025384999728 1104 1 0.29174924815797 1104 2 0.2559988297688989 1104 3 0.2824073731771044 1104 4 0.3546805561054498 1104 5 0.23525476317330885 1104 6 0.265081379710395 1104 7 0.2647495058363134 1104 8 0.3321169707143454 1104 9 0.495955398332363 1104 10-11 0.4268084519969335 1104 12-13 0.2703862225736202 1104 14-15 0.386572467640967 1104 16-17 0.4536423196582149 1104 18-19 0.4173187187899501 1104 20-21 0.38884124977578693 1104 22-23 0.34061921640861925 1104 24-25 0.453229643436039 1104 26-27 0.4507139698708116 1104 28-29 0.36818800038668087 1104 30-31 0.4288946075424036 1104 32-33 0.4542679149314921 1104 34-35 0.4209125914432619 1104 36-37 0.39705210197584506 1104 38-39 0.284608533224322 1104 40-41 0.3806097148148524 1104 42-43 0.3806795571535062 1104 44-45 0.388245617952947 1104 46-47 0.3387963230999844 1104 48-49 0.339640704111126 1104 50-51 0.3192237250651857 1104 52-53 0.3318685726320396 1104 54-55 0.3891354660629105 1104 56-57 0.28620477248832543 1104 58-59 0.3717866044657754 1104 60-61 0.2148641371284583 1104 62-63 0.27124191887996574 1104 64-65 0.2961969119006653 1104 66-67 0.27219022386239544 1104 68-69 0.25669230265979337 1104 70-71 0.16320191287521624 1104 72-73 0.1693073921264201 1104 74-75 0.061861186129569035 1104 76-77 0.14215754698038552 1104 78-79 0.14494790225324294 1104 80-81 0.16553425940531952 1104 82-83 0.08845440249746872 1104 84-85 0.10694286250472373 1104 86-87 0.07431338702555124 1104 88-89 0.07908679795244566 1104 90-91 0.05926127738809939 1104 92-93 0.08513102724887744 1104 94-95 0.1499696184635404 1104 96-97 0.09771352446113468 1104 98-99 0.03594911773729237 1104 100-101 0.00150688051676795 1104 102-103 -0.05234180626132812 1104 104-105 0.07685761533813462 1104 106-107 0.0787879089577217 1104 108-109 -0.014485004561773707 1104 110-111 0.0031750580136566953 1104 112-113 -0.004811588858721905 1104 114-115 -0.04858832674737101 1104 116-117 -0.19992810143619977 1104 118-119 -0.1525271693211856 1104 120-121 -0.1680707968389079 1104 122-123 -0.34232862561930943 1104 124-125 -0.37448460367144776 1105 1 0.20526870040213296 1105 2 0.08220673446742666 1105 3 0.05687600959855743 1105 4 0.16719287799129035 1105 5 0.18285049262838982 1105 6 0.260591799035204 1105 7 0.16573097180811658 1105 8 0.28572763612260843 1105 9 0.2883884305346456 1105 10-11 0.3396245515972467 1105 12-13 0.4772851515634571 1105 14-15 0.5210257369205493 1105 16-17 0.46405804560664876 1105 18-19 0.37225764553499374 1105 20-21 0.48853743832198404 1105 22-23 0.4318549296283294 1105 24-25 0.40530044565280576 1105 26-27 0.4101440517932815 1105 28-29 0.3822021387420165 1105 30-31 0.3988666005214725 1105 32-33 0.4865289597554323 1105 34-35 0.43930305877690046 1105 36-37 0.3577017483482692 1105 38-39 0.3454685555890009 1105 40-41 0.32232822103060244 1105 42-43 0.3320511679109117 1105 44-45 0.2850738844068559 1105 46-47 0.34624141317782176 1105 48-49 0.21910474129906987 1105 50-51 0.3243495733423316 1105 52-53 0.34688370078696096 1105 54-55 0.3014760097542819 1105 56-57 0.32621866634595875 1105 58-59 0.22997326822935094 1105 60-61 0.15141658902769706 1105 62-63 0.17940587044873268 1105 64-65 0.14465984345063987 1105 66-67 0.10766441164135188 1105 68-69 0.1347092035917683 1105 70-71 0.21907391214104877 1105 72-73 0.13826051345903068 1105 74-75 0.0853131158451319 1105 76-77 0.17946374684162691 1105 78-79 0.20085094170611484 1105 80-81 0.1775443375692305 1105 82-83 0.18725902914980708 1105 84-85 0.2088675902760997 1105 86-87 0.204051022093946 1105 88-89 0.25643777440812343 1105 90-91 0.2168677708152842 1105 92-93 0.22133666129024832 1105 94-95 0.24485182264071526 1105 96-97 0.246421943403341 1105 98-99 0.20251818400910082 1105 100-101 0.12391981869652469 1105 102-103 0.09160503674359077 1105 104-105 0.11602133421795813 1105 106-107 0.2144934136087464 1105 108-109 0.17167086958556155 1105 110-111 0.22683445089234056 1105 112-113 0.20542629706161364 1105 114-115 0.1281513492133186 1105 116-117 0.10989378974567643 1105 118-119 0.18102567586621987 1105 120-121 0.15415879055490578 1105 122-123 0.1965362752281017 1105 124-125 0.0430545527934747 1106 1 0.4130502594641925 1106 2 0.4309574214838747 1106 3 0.3215199749027562 1106 4 0.4135359455649379 1106 5 0.2692320505572212 1106 6 0.3208971328979615 1106 7 0.25733712363994954 1106 8 0.3543855862703893 1106 9 0.4999552813209931 1106 10-11 0.5200234657080927 1106 12-13 0.41012267440770245 1106 14-15 0.4988175080652937 1106 16-17 0.5647360834397404 1106 18-19 0.5381255897507842 1106 20-21 0.43557920325579147 1106 22-23 0.46198455072649836 1106 24-25 0.4670127739422796 1106 26-27 0.5829426353516993 1106 28-29 0.47153563363227136 1106 30-31 0.5415882469550226 1106 32-33 0.5635297194507061 1106 34-35 0.5697724397451083 1106 36-37 0.6137066316677107 1106 38-39 0.5034524276463799 1106 40-41 0.582467624894278 1106 42-43 0.5354867077892393 1106 44-45 0.5246967761217292 1106 46-47 0.3764391911018521 1106 48-49 0.4040220552467346 1106 50-51 0.3767386362584375 1106 52-53 0.4554878163042204 1106 54-55 0.43802970861595725 1106 56-57 0.39222095196958406 1106 58-59 0.2922741097346062 1106 60-61 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-1.7021038334303782 2216 70-71 -1.8079932605799698 2216 72-73 -1.6412239839539815 2216 74-75 -1.5537572026365503 2216 76-77 -1.6550034073180484 2216 78-79 -1.7778398166793608 2216 80-81 -1.6182842909796555 2216 82-83 -1.6361213971423325 2216 84-85 -1.6408040093248353 2216 86-87 -1.6253582632170733 2216 88-89 -1.5190080361424485 2216 90-91 -1.6280690916219775 2216 92-93 -1.729654001968349 2216 94-95 -1.6069250874373218 2216 96-97 -1.5332935557033949 2216 98-99 -1.6584477017922445 2216 100-101 -1.679631342934087 2216 102-103 -1.559710063294304 2216 104-105 -1.6567316800665424 2216 106-107 -1.5156969948181036 2216 108-109 -1.4871811984572254 2216 110-111 -1.4774316124018334 2216 112-113 -1.4905528040280913 2216 114-115 -1.4460339819132741 2216 116-117 -1.4722395657581444 2216 118-119 -1.3368741760297453 2216 120-121 -1.2368287598120666 2216 122-123 -1.357319070051517 2216 124-125 -1.272400664103536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32933.0 3 5961.0 4 3760.0 5 3141.0 6 3264.0 7 3976.0 8 4344.0 9 4803.0 10 5241.0 11 6105.0 12 6770.0 13 6767.0 14 6719.0 15 6168.0 16 5554.0 17 5048.0 18 4848.0 19 4753.0 20 5020.0 21 5172.0 22 6195.0 23 8394.0 24 12263.0 25 17205.0 26 20499.0 27 21472.0 28 25454.0 29 30893.0 30 36878.0 31 45100.0 32 58410.0 33 77457.0 34 109050.0 35 166269.0 36 325977.0 37 627346.0 38 266423.0 39 555.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59209239209824 18.239259186874584 12.335652739817798 24.832995681209383 2 15.786730769424434 20.012558044449634 39.01483299412179 25.18587819200414 3 18.44465054080154 24.567172475462282 29.50014248844233 27.488034495293856 4 12.44509229328419 16.08788254131295 37.15361066634442 34.31341449905844 5 13.319037779643338 38.425008019845905 34.72006583072258 13.535888369788172 6 32.858258040471796 36.64773013426807 17.227812528329252 13.266199296930873 7 29.03692596931991 32.29535740485542 21.222148431934 17.44556819389067 8 26.774206216543885 35.347127959947315 19.914871935329252 17.963793888179545 9 26.693408022507448 15.699780534259865 19.380853867234052 38.225957575998635 10-11 24.963189509142268 26.569609651460226 27.961825337147346 20.505375502250168 12-13 25.348472360609797 23.865919768156687 27.951397892122976 22.83420997911054 14-15 22.39402631286134 25.628833684007013 24.798066838957457 27.179073164174188 16-17 22.901246082627267 28.903508208829543 25.712817716581714 22.48242799196147 18-19 22.996045182095322 27.775509330701826 26.89739374730954 22.33105173989331 20-21 23.31472900455219 27.303327255522074 26.759345106816507 22.622598633109227 22-23 23.311828141056203 27.079449216010374 26.631782405181387 22.976940237752032 24-25 23.23874841593465 27.330910936382125 26.567614227663356 22.862726420019865 26-27 22.90419930459685 27.574884728821974 26.436950013744937 23.08396595283624 28-29 23.02250276095527 27.419841670826667 26.650474768506456 22.907180799711607 30-31 22.88494279097857 27.21337400433948 26.60590182719958 23.29578137748237 32-33 22.691634483923565 27.394573562815317 26.611060861194346 23.302731092066768 34-35 23.36673189291818 27.288085799582966 26.19842223462469 23.146760072874162 36-37 23.41167037772209 26.997846371116452 26.20980196935579 23.380681281805675 38-39 22.937533213824402 27.149354528420965 26.624856162585182 23.288256095169455 40-41 23.08265306867394 26.91615116589647 26.78622810522031 23.214967660209282 42-43 22.974679369861647 26.884874232629986 26.602118552971625 23.538327844536738 44-45 22.91851487850388 27.13258891304501 26.65850684287321 23.290389365577894 46-47 23.258138145637382 27.361151634048465 26.339596004705502 23.041114215608655 48-49 23.06482219448622 27.1100858076304 26.410932102566377 23.414159895317006 50-51 23.00435457487135 27.128286510786747 26.593795045481617 23.273563868860283 52-53 23.29147702098522 26.95767209086881 26.45398658718331 23.29686430096266 54-55 22.796299708878745 27.084950813498114 26.592380900592815 23.526368577030322 56-57 22.913500001258694 27.469344497113813 26.578793012735467 23.038362488892023 58-59 22.915758827594278 26.830650409267072 26.636357113996894 23.617233649141752 60-61 22.732918597941964 27.04695881411725 26.97191473421658 23.24820785372421 62-63 22.75540361600467 27.1783130346345 26.86139655719299 23.20488679216784 64-65 22.685188215421253 27.09592151669935 26.638404354141542 23.58048591373785 66-67 22.597136139124828 27.381364764642797 26.513118476635466 23.508380619596906 68-69 22.640743742128176 26.887494376594347 26.95989690443949 23.511864976837987 70-71 22.725147779958718 26.897274142302262 26.79285077553482 23.584727302204193 72-73 22.763370685592527 27.12774955994957 26.50538533111529 23.60349442334261 74-75 22.82011926109177 27.3159356573143 26.559974301996352 23.303970779597577 76-77 22.980897062447873 27.046102266156236 26.623553333177252 23.34944733821864 78-79 22.911623373223183 27.047946364196612 26.682135563727762 23.358294698852443 80-81 22.939950190304454 27.30432777427996 26.609871966476007 23.14585006893958 82-83 22.92549381915765 27.261007639045353 26.56331068094367 23.250187860853323 84-85 22.854225542513714 27.12324366834477 26.6694811272544 23.35304966188712 86-87 23.11101119658111 27.058916230561564 26.7101244523302 23.11994812052713 88-89 22.965105301398093 27.136427330278373 26.596187126175884 23.30228024214765 90-91 22.9070119000178 27.318006206406437 26.68442610879664 23.090555784779124 92-93 23.0355269173217 26.81763164625538 26.820476337206138 23.32636509921678 94-95 22.79990695641754 26.830587171465027 26.956155729178725 23.413350142938707 96-97 23.142809168460943 26.739364060178882 26.77996967543451 23.337857095925667 98-99 23.001416294383812 26.887289047407346 26.531251872323054 23.580042785885794 100-101 22.757957826114538 26.778131447749875 26.958227895263114 23.50568283087247 102-103 22.835180869205335 26.58926098061636 26.917985921570224 23.657572228608082 104-105 22.778122103406105 26.812471647679526 26.831805349897213 23.577600899017153 106-107 22.918895240157248 26.821505582080945 26.596636275406997 23.66296290235481 108-109 23.02604367406299 26.758274637117292 26.411925214907576 23.803756473912145 110-111 22.941154406087538 27.07615289940793 26.58820016861721 23.394492525887323 112-113 22.868232568759648 27.055073422586883 26.88985340657089 23.186840602082583 114-115 22.829694591380406 27.202151323732732 26.5129453015569 23.455208783329958 116-117 22.739759202355916 27.191112046477883 26.685535677333373 23.383593073832827 118-119 22.698964831053708 27.328626409564826 26.377301589260497 23.595107170120965 120-121 22.74065763859586 27.511563711949016 26.45539624784864 23.29238240160648 122-123 22.811524311249652 27.631455059848538 26.419303790298258 23.137716838603552 124-125 22.882753971216967 27.67492449533277 26.303920351859176 23.138401181591085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 605.0 1 529.5 2 393.5 3 298.0 4 239.5 5 207.0 6 189.5 7 176.0 8 185.0 9 189.0 10 170.5 11 164.0 12 163.0 13 154.0 14 178.0 15 218.0 16 255.5 17 324.0 18 432.5 19 539.5 20 712.5 21 1010.5 22 1451.5 23 2175.0 24 3173.0 25 4455.5 26 6357.5 27 8812.0 28 11315.0 29 14470.5 30 19072.5 31 23705.0 32 29289.5 33 33472.0 34 36348.0 35 40084.5 36 45405.5 37 50917.0 38 54179.5 39 56825.5 40 58348.5 41 61887.0 42 66086.5 43 70370.5 44 74946.0 45 78494.0 46 78134.5 47 76321.5 48 77054.0 49 75550.0 50 73768.5 51 72359.5 52 72052.5 53 72913.0 54 73967.5 55 70219.5 56 61482.5 57 56136.5 58 52803.0 59 46460.5 60 37688.5 61 30361.5 62 24773.0 63 18960.5 64 13959.0 65 10650.5 66 8053.0 67 6615.5 68 5116.0 69 3409.0 70 2357.5 71 1692.5 72 1353.0 73 1038.5 74 728.0 75 560.5 76 373.0 77 195.5 78 108.5 79 75.0 80 48.0 81 31.0 82 25.5 83 20.0 84 15.5 85 14.0 86 11.5 87 9.0 88 6.0 89 6.5 90 7.5 91 4.0 92 3.5 93 4.5 94 3.5 95 3.5 96 2.5 97 1.0 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04576608345538462 2 0.010522674853878311 3 0.0030712113209884068 4 0.0063941612748447145 5 0.024569690567907254 6 0.030561070030163326 7 0.003927122672739274 8 1.5104317972074128E-4 9 0.0 10-11 2.5173863286790216E-5 12-13 9.062590783244478E-4 14-15 5.034772657358044E-4 16-17 1.258693164339511E-4 18-19 1.0069545314716086E-4 20-21 2.5173863286790216E-5 22-23 0.0 24-25 0.0 26-27 5.034772657358043E-5 28-29 4.279556758754337E-4 30-31 2.769124961546924E-4 32-33 1.762170430075315E-4 34-35 0.0011831715744791403 36-37 2.2656476958111195E-4 38-39 1.762170430075315E-4 40-41 5.78998855596175E-4 42-43 0.0010069545314716087 44-45 0.0016614749769281543 46-47 2.5173863286790216E-5 48-49 0.0 50-51 0.0 52-53 0.0013593886174866716 54-55 0.003675384039871372 56-57 1.0069545314716086E-4 58-59 7.300420353169162E-4 60-61 0.0010573022580451891 62-63 8.559113517508674E-4 64-65 0.0016111272503545738 66-67 0.0028698204146940846 68-69 0.00344881927029026 70-71 0.0020894306528035883 72-73 0.002567734055252602 74-75 0.002039082926230008 76-77 0.0013342147541998814 78-79 0.002567734055252602 80-81 0.0013845624807734618 82-83 4.7830340244901413E-4 84-85 8.055636251772869E-4 86-87 0.0022656476958111196 88-89 0.006343813548271135 90-91 6.796943087433358E-4 92-93 0.0015607795237809934 94-95 0.0010573022580451891 96-97 4.279556758754337E-4 98-99 9.062590783244478E-4 100-101 0.0014600840706338325 102-103 0.001409736344060252 104-105 9.062590783244478E-4 106-107 0.0072752464898823725 108-109 0.0021397783793771687 110-111 0.0015859533870677838 112-113 0.0023915170122450705 114-115 0.0030712113209884068 116-117 0.0019132136097960565 118-119 0.04810725274105611 120-121 0.011731020291644242 122-123 0.004531295391622239 124-125 0.0025929079185393923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1986187.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.31513200460257 #Duplication Level Percentage of deduplicated Percentage of total 1 84.57167184679929 56.92953254217225 2 9.480455682837189 12.7635625150794 3 2.730716472300882 5.514556194002296 4 1.109015436403618 2.9861408198660584 5 0.5864419962103522 1.9738210193971248 6 0.32440700920086823 1.310250038854486 7 0.19972980894607104 0.9411386918121177 8 0.159680829094572 0.8599148871284398 9 0.10484330337454839 0.6351786725810687 >10 0.621765884638446 8.070335875492244 >50 0.07527483385708772 3.5223329716743184 >100 0.03479125870263566 3.9761455604569202 >500 0.0012056376344219836 0.5170902114833048 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.034772657358043E-5 0.0 10-11 0.0 0.0 0.0 5.034772657358043E-5 0.0 12-13 0.0 0.0 0.0 5.034772657358043E-5 0.0 14-15 0.0 0.0 0.0 5.034772657358043E-5 0.0 16-17 0.0 0.0 0.0 5.034772657358043E-5 0.0 18-19 0.0 0.0 0.0 5.034772657358043E-5 0.0 20-21 0.0 0.0 0.0 1.258693164339511E-4 0.0 22-23 0.0 0.0 0.0 2.517386328679022E-4 0.0 24-25 0.0 0.0 0.0 4.531295391622239E-4 0.0 26-27 0.0 0.0 0.0 6.293465821697554E-4 0.0 28-29 0.0 0.0 0.0 0.002995689731128036 0.0 30-31 0.0 0.0 0.0 0.011152021436048066 0.0 32-33 0.0 0.0 0.0 0.029831027994846403 0.0 34-35 0.0 0.0 0.0 0.05465245719562156 0.0 36-37 0.0 0.0 0.0 0.09915984748666666 0.0 38-39 0.0 0.0 0.0 0.17082983626415843 0.0 40-41 0.0 0.0 0.0 0.24954850676195145 0.0 42-43 0.0 0.0 0.0 0.3221499284810544 0.0 44-45 0.0 0.0 0.0 0.41383313857154436 0.0 46-47 0.0 0.0 0.0 0.5266623938229381 0.0 48-49 0.0 0.0 0.0 0.6510464523229686 0.0 50-51 0.0 0.0 0.0 0.7882440072359753 0.0 52-53 0.0 0.0 0.0 0.9163286236391639 0.0 54-55 0.0 0.0 0.0 1.0506311842741898 0.0 56-57 0.0 0.0 0.0 1.215947944478541 0.0 58-59 0.0 0.0 0.0 1.3962683272018195 0.0 60-61 0.0 0.0 0.0 1.5958970630660658 0.0 62-63 0.0 0.0 0.0 1.7929580648750596 0.0 64-65 0.0 0.0 0.0 1.9944748404858152 0.0 66-67 0.0 0.0 0.0 2.229900809943877 0.0 68-69 0.0 0.0 0.0 2.4925648994782463 0.0 70-71 0.0 0.0 0.0 2.776148469403938 0.0 72-73 0.0 0.0 0.0 3.061343166579985 0.0 74-75 0.0 0.0 0.0 3.3522523307221324 0.0 76-77 0.0 0.0 0.0 3.661387371883916 0.0 78-79 0.0 0.0 0.0 4.02071909643956 0.0 80-81 0.0 0.0 0.0 4.409504241040748 0.0 82-83 0.0 0.0 0.0 4.803575896932163 0.0 84-85 0.0 0.0 0.0 5.187754224551867 0.0 86-87 0.0 0.0 0.0 5.584292919045387 0.0 88-89 0.0 0.0 0.0 6.019976970949865 0.0 90-91 0.0 0.0 0.0 6.498809024527901 0.0 92-93 0.0 0.0 0.0 6.998031907368238 0.0 94-95 0.0 0.0 0.0 7.498589004962776 0.0 96-97 0.0 0.0 0.0 7.994212025353101 0.0 98-99 0.0 0.0 0.0 8.516720731733718 0.0 100-101 0.0 0.0 0.0 9.102592052007187 0.0 102-103 0.0 0.0 0.0 9.708224854960786 0.0 104-105 0.0 0.0 0.0 10.319974906693076 0.0 106-107 0.0 0.0 0.0 10.906626616728435 0.0 108-109 0.0 0.0 0.0 11.516438280987641 0.0 110-111 0.0 0.0 0.0 12.183847744447023 0.0 112-113 0.0 0.0 0.0 12.88408392563238 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1725 0.0 51.418056 1 GTATCAA 2545 0.0 43.97325 1 GTACATG 7505 0.0 40.610447 1 CAACGCA 2715 0.0 39.675083 5 ATCAACG 2715 0.0 39.675083 3 TCAACGC 2755 0.0 39.09904 4 ACATGGG 7840 0.0 37.2713 3 AACGCAG 2895 0.0 37.206364 6 TACATGG 8135 0.0 36.943916 2 TATCAAC 3350 0.0 32.509884 2 CATGGGG 4975 0.0 31.93944 4 ACGCAGA 3595 0.0 30.120441 7 CGCAGAG 3680 0.0 29.101376 8 CATGGGA 3995 0.0 28.154911 4 ATGGGGA 2815 0.0 27.272215 5 GAGTACA 5740 0.0 26.65261 1 GTATAGG 790 0.0 26.372995 1 ATGGGAT 2225 0.0 22.73516 5 AGTACAT 6250 0.0 22.662409 2 GCAGAGT 4605 0.0 22.22223 9 >>END_MODULE