FastQCFastQC Report
Thu 26 May 2016
SRR1364337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986187
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.15043900700185833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28980.1459077116102361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.066.534141
GTATCAA44650.047.7443051
ATCAACG54250.038.629173
TCAACGC55150.037.8908234
GTACATG67700.037.8215871
CAACGCA56550.036.9527665
AACGCAG58450.035.836276
TATCAAC58600.035.6600532
TACATGG75900.033.1795922
ACATGGG72600.033.0476763
ACGCAGA69000.030.2661427
CGCAGAG69350.030.1133948
GTGGTAT15350.027.542921
CATGGGG43750.027.4881674
GAGTACA53400.026.0936361
CATGGGA40950.025.7330844
TGGTATC16250.025.2794822
GCAGAGT82300.024.9412569
ATGGGGA26650.024.7969475
CAGAGTA79900.023.86603710-11
Produced by FastQC (version 0.11.5)