##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364337_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1986187 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31786634390417 33.0 31.0 34.0 30.0 34.0 2 32.334220795927074 34.0 31.0 34.0 30.0 34.0 3 32.44438766339725 34.0 31.0 34.0 30.0 34.0 4 36.046337530151995 37.0 35.0 37.0 35.0 37.0 5 35.978596677956304 37.0 35.0 37.0 35.0 37.0 6 36.032517079207544 37.0 35.0 37.0 35.0 37.0 7 35.985896091354945 37.0 35.0 37.0 35.0 37.0 8 35.992678937078935 37.0 35.0 37.0 35.0 37.0 9 37.75439875500142 39.0 38.0 39.0 35.0 39.0 10-11 37.70738832748377 39.0 37.5 39.0 35.0 39.0 12-13 37.68138850974253 39.0 37.5 39.0 35.0 39.0 14-15 39.100667510158914 41.0 38.0 41.0 36.0 41.0 16-17 39.108225710872134 41.0 38.5 41.0 36.0 41.0 18-19 39.14930190359719 41.0 39.0 41.0 36.0 41.0 20-21 39.1518218073122 41.0 39.0 41.0 35.5 41.0 22-23 39.11276355146822 41.0 39.0 41.0 35.0 41.0 24-25 39.081512969322624 41.0 39.0 41.0 35.0 41.0 26-27 38.994978317751546 41.0 39.0 41.0 35.0 41.0 28-29 38.90807134474247 40.5 38.0 41.0 35.0 41.0 30-31 38.81955928621021 40.0 38.0 41.0 35.0 41.0 32-33 38.70822661713122 40.0 38.0 41.0 35.0 41.0 34-35 38.62351279109167 40.0 38.0 41.0 34.0 41.0 36-37 38.517685897652136 40.0 38.0 41.0 34.0 41.0 38-39 38.43706936960115 40.0 38.0 41.0 34.0 41.0 40-41 38.3413976126115 40.0 38.0 41.0 34.0 41.0 42-43 38.20704722163623 40.0 38.0 41.0 33.5 41.0 44-45 38.068690410318865 40.0 38.0 41.0 33.0 41.0 46-47 37.876021492437516 40.0 38.0 41.0 33.0 41.0 48-49 37.68588556868009 40.0 37.0 41.0 33.0 41.0 50-51 37.53287077198672 40.0 37.0 41.0 32.5 41.0 52-53 37.38905223929066 40.0 37.0 41.0 32.0 41.0 54-55 37.20524603171806 40.0 36.0 41.0 32.0 41.0 56-57 37.00890852673993 39.5 36.0 41.0 31.0 41.0 58-59 36.79172580426717 39.0 35.5 41.0 31.0 41.0 60-61 36.53754228579685 39.0 35.0 41.0 31.0 41.0 62-63 36.28859895870832 38.5 35.0 41.0 31.0 41.0 64-65 35.99483507846945 38.0 35.0 40.0 30.5 41.0 66-67 35.66322632259702 37.0 35.0 40.0 30.0 41.0 68-69 35.32381794866244 37.0 35.0 39.5 30.0 41.0 70-71 35.061863258595494 36.0 34.5 39.0 29.5 41.0 72-73 34.85606088449879 36.0 34.5 39.0 30.0 41.0 74-75 34.52152843614423 35.5 34.0 38.5 30.0 40.0 76-77 34.18536019015329 35.0 34.0 37.0 29.0 39.0 78-79 33.870824348361964 35.0 34.0 37.0 29.0 39.0 80-81 33.58069960179983 35.0 34.0 36.0 29.0 39.0 82-83 33.31309161725457 35.0 34.0 36.0 29.0 37.0 84-85 33.08470249780106 35.0 34.0 36.0 29.0 37.0 86-87 32.90104682993092 35.0 34.0 35.0 29.0 36.5 88-89 32.74941508528653 35.0 34.0 35.0 29.0 36.0 90-91 32.613871704930105 35.0 34.0 35.0 29.0 36.0 92-93 32.49846439433951 35.0 34.0 35.0 29.0 36.0 94-95 32.42086822640567 35.0 33.0 35.0 29.0 35.5 96-97 32.34224496484973 35.0 33.0 35.0 27.5 35.0 98-99 32.27916001866893 35.0 33.0 35.0 27.0 35.0 100-101 32.2224425494679 35.0 33.0 35.0 27.0 35.0 102-103 31.247426350086876 34.0 31.5 35.0 25.0 35.0 104-105 31.96843675847239 35.0 33.0 35.0 26.5 35.0 106-107 32.26153025873193 35.0 33.5 35.0 28.0 35.0 108-109 32.28123510021967 35.0 34.0 35.0 29.0 35.0 110-111 32.250244815820466 35.0 34.0 35.0 29.0 35.0 112-113 32.18845632359894 35.0 34.0 35.0 28.0 35.0 114-115 32.11466820596449 35.0 34.0 35.0 27.0 35.0 116-117 32.058292346088265 35.0 34.0 35.0 27.0 35.0 118-119 31.962047883708834 35.0 34.0 35.0 27.0 35.0 120-121 31.83435749000472 35.0 33.5 35.0 27.0 35.0 122-123 31.698163365282323 35.0 33.0 35.0 26.0 35.0 124-125 31.01964920724987 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11972715795128863 1101 2 0.1473189817778504 1101 3 0.1766580138447651 1101 4 0.13373071479460918 1101 5 0.22721619341842825 1101 6 0.20612717927211577 1101 7 0.2608560437105396 1101 8 0.22204762303802994 1101 9 0.17066166253177073 1101 10-11 0.225653918006401 1101 12-13 0.21963805138312154 1101 14-15 0.3186830737875468 1101 16-17 0.3142244878186631 1101 18-19 0.29633226641375643 1101 20-21 0.22730809493782544 1101 22-23 0.22427112593017284 1101 24-25 0.22707539490322404 1101 26-27 0.2263925583588744 1101 28-29 0.16083063718992463 1101 30-31 0.22474210807766326 1101 32-33 0.22230037001780545 1101 34-35 0.24027227710048749 1101 36-37 0.23692191674918917 1101 38-39 0.26523853934404684 1101 40-41 0.25883040860325934 1101 42-43 0.29131662617396614 1101 44-45 0.09529615644974854 1101 46-47 0.18088217244049787 1101 48-49 0.041832877015998804 1101 50-51 0.1491534487093631 1101 52-53 0.11814815315241134 1101 54-55 0.07702808696227947 1101 56-57 0.1130818252209167 1101 58-59 -0.09324351540718112 1101 60-61 -0.037149994366835415 1101 62-63 -0.11296914941750913 1101 64-65 -0.2173856392276292 1101 66-67 -0.22725138609160211 1101 68-69 -0.15349290431304752 1101 70-71 -0.08840114774521624 1101 72-73 -0.19635227100067 1101 74-75 -0.08847151507877982 1101 76-77 -0.09453627175032864 1101 78-79 -0.2904603776576309 1101 80-81 -0.22900282258473226 1101 82-83 -0.3119207589447086 1101 84-85 -0.4365473705994489 1101 86-87 -0.3352763600421014 1101 88-89 -0.39569434883615173 1101 90-91 -0.9520894116208467 1101 92-93 -0.7257943681780112 1101 94-95 -0.4774730976393542 1101 96-97 -0.3536734213163655 1101 98-99 -0.35543764530568467 1101 100-101 -0.4138066836437524 1101 102-103 -0.13143518685351907 1101 104-105 -0.24522118773408863 1101 106-107 -0.2389355469767409 1101 108-109 -0.13075330149936804 1101 110-111 -0.17171045935773321 1101 112-113 -0.24734146684080116 1101 114-115 -0.4168893540877079 1101 116-117 -0.2556234301069402 1101 118-119 -0.201896246095572 1101 120-121 -0.23952113177578838 1101 122-123 -0.2592634446958719 1101 124-125 -0.2302238799385421 1102 1 0.07786714238489623 1102 2 0.1485175327180457 1102 3 0.09245446189601125 1102 4 0.13081851107049403 1102 5 0.03792970052212041 1102 6 0.09468779243305647 1102 7 0.15218279834225257 1102 8 0.16937352498199232 1102 9 0.23871661250131382 1102 10-11 0.22544170114051099 1102 12-13 0.19161873958465492 1102 14-15 0.3347549516973274 1102 16-17 0.32588151632920415 1102 18-19 0.3218962169761994 1102 20-21 0.26347703102393893 1102 22-23 0.3453990754453571 1102 24-25 0.257125379520204 1102 26-27 0.2780321867418678 1102 28-29 0.23261991634787194 1102 30-31 0.2887040677109951 1102 32-33 0.19992164625807618 1102 34-35 0.23318189067094863 1102 36-37 0.3262240218793764 1102 38-39 0.37237106352390725 1102 40-41 0.2998875911477157 1102 42-43 0.1530842392187708 1102 44-45 0.27058754236787763 1102 46-47 0.23152827187794855 1102 48-49 0.18765693554481544 1102 50-51 0.15723551761305288 1102 52-53 0.21658774475879028 1102 54-55 0.11130502659768382 1102 56-57 0.1291472086451506 1102 58-59 0.1831525570335799 1102 60-61 0.15997929021082768 1102 62-63 0.10443623738895269 1102 64-65 0.0622743578995113 1102 66-67 -0.046194616138002687 1102 68-69 0.062185204024139296 1102 70-71 -0.07085750480234765 1102 72-73 -0.03567920798735713 1102 74-75 0.04023161511044293 1102 76-77 -0.013029841445579393 1102 78-79 0.07809110756753768 1102 80-81 0.0023695921006492426 1102 82-83 0.0025520365467457395 1102 84-85 -0.030031287706385967 1102 86-87 -0.050111204001261456 1102 88-89 -0.08845488212764963 1102 90-91 -0.08988775045878583 1102 92-93 -0.04811791427918166 1102 94-95 -0.13450511010469057 1102 96-97 -0.058003816934956376 1102 98-99 0.004296366034964194 1102 100-101 -0.012232203864783742 1102 102-103 -0.023640925533758406 1102 104-105 -0.08104492281205111 1102 106-107 -0.1375926019745961 1102 108-109 -0.040169808902568604 1102 110-111 -0.03877840033585045 1102 112-113 -0.06431915993841386 1102 114-115 -0.06247903809405386 1102 116-117 -0.13258062609879673 1102 118-119 -0.16087223636453984 1102 120-121 -0.08187373496934214 1102 122-123 -0.11221014559908937 1102 124-125 -0.27862982756293775 1103 1 0.08719847745220477 1103 2 0.09686527429050784 1103 3 0.15477394902303132 1103 4 0.1306879220574828 1103 5 0.0066453932390402315 1103 6 0.004742457830062108 1103 7 0.06935517977431971 1103 8 0.07919185131778761 1103 9 0.22488376840541235 1103 10-11 0.204976976405419 1103 12-13 0.23704268906859483 1103 14-15 0.2811998072280062 1103 16-17 0.24920227934285322 1103 18-19 0.24818572814537276 1103 20-21 0.20155767256767376 1103 22-23 0.23686226754253425 1103 24-25 0.13724294406216586 1103 26-27 0.1705634543258867 1103 28-29 0.18931059893246527 1103 30-31 0.17100241958419105 1103 32-33 0.17705799397603528 1103 34-35 0.16604868594279765 1103 36-37 0.16686779866118684 1103 38-39 0.22625991087789288 1103 40-41 0.0828072122575918 1103 42-43 0.09297037838780398 1103 44-45 0.12870958874442806 1103 46-47 0.1894627471806345 1103 48-49 0.032296465601440616 1103 50-51 -0.08535976563968717 1103 52-53 0.037776709765857674 1103 54-55 0.023490555465976115 1103 56-57 -0.11175004854290194 1103 58-59 0.010904050322629644 1103 60-61 0.09749232451909506 1103 62-63 0.081153396834452 1103 64-65 0.09647281680160091 1103 66-67 -0.08024059822640339 1103 68-69 -0.11740997987273971 1103 70-71 -0.036865750354948545 1103 72-73 0.007396967461325232 1103 74-75 0.13729196051469472 1103 76-77 0.09858736698924275 1103 78-79 0.02473135475705135 1103 80-81 0.09588878647219445 1103 82-83 0.06268569477046526 1103 84-85 0.053089233902042565 1103 86-87 0.1248855743112216 1103 88-89 0.0743640403433048 1103 90-91 0.04232661620171285 1103 92-93 0.09441433702621538 1103 94-95 0.00777825969191781 1103 96-97 -0.028147627163292555 1103 98-99 0.12166588523129462 1103 100-101 0.037343787627996505 1103 102-103 0.1296424167186494 1103 104-105 0.10866033458847824 1103 106-107 0.05967415276793275 1103 108-109 0.09127614839605513 1103 110-111 0.15702273251768872 1103 112-113 0.12016585700127536 1103 114-115 0.034527940425405745 1103 116-117 0.03486141795617925 1103 118-119 -0.07293885791118271 1103 120-121 -0.01062968156507793 1103 122-123 0.046249046686959616 1103 124-125 -0.08726576998769175 1104 1 0.10682837574709225 1104 2 0.051044797306239786 1104 3 0.10381710975227776 1104 4 0.06693915455191757 1104 5 0.10743851765243306 1104 6 0.1555701945317267 1104 7 0.1453203469434996 1104 8 0.18242279965304675 1104 9 0.14269495037304125 1104 10-11 0.12157511680354105 1104 12-13 0.1933158123677643 1104 14-15 0.18644743069787495 1104 16-17 0.25706952469965927 1104 18-19 0.15396646373073963 1104 20-21 0.12335249064653908 1104 22-23 0.11860018196051669 1104 24-25 0.08983826713282639 1104 26-27 0.10318396482893633 1104 28-29 0.12086638157880714 1104 30-31 0.14500694508045342 1104 32-33 0.08390029201098059 1104 34-35 0.07165319467966924 1104 36-37 0.16413179338658068 1104 38-39 0.024399365134080142 1104 40-41 -0.022548357351098502 1104 42-43 0.005805310117452223 1104 44-45 0.04491809629985255 1104 46-47 -0.029172614722085655 1104 48-49 -0.11281327963968835 1104 50-51 -0.08900430031678042 1104 52-53 0.07762924323110809 1104 54-55 0.0061183406501683635 1104 56-57 0.015998129160983865 1104 58-59 0.029875198614931264 1104 60-61 0.03543158231284593 1104 62-63 -0.039232907150996255 1104 64-65 0.02147344412774288 1104 66-67 -0.08096777857610249 1104 68-69 -0.11556345400491352 1104 70-71 -0.09739869441609983 1104 72-73 0.06251436032609803 1104 74-75 0.03797846250498793 1104 76-77 0.09434652715953717 1104 78-79 0.16323063638228774 1104 80-81 0.19841989206488364 1104 82-83 0.1875142160326746 1104 84-85 0.2509596400617653 1104 86-87 0.11394529414852173 1104 88-89 0.1651406079507609 1104 90-91 0.15482023646750775 1104 92-93 0.1706144925110138 1104 94-95 0.11291058176783508 1104 96-97 0.07405093802095308 1104 98-99 0.06505683741569612 1104 100-101 0.046209690161546746 1104 102-103 0.05399833693934042 1104 104-105 0.10108902602298286 1104 106-107 0.035877485170438206 1104 108-109 0.08064038040036792 1104 110-111 0.08848660325121216 1104 112-113 0.03826336443307099 1104 114-115 0.06071264872335291 1104 116-117 -0.05213133705995432 1104 118-119 -0.013194100228282934 1104 120-121 -0.04310025536476303 1104 122-123 -0.09971927207667619 1104 124-125 -0.3120340794507861 1105 1 0.0652872709481116 1105 2 0.10390342421207777 1105 3 0.10641526337848717 1105 4 0.05495494321053229 1105 5 0.024658162626920443 1105 6 0.03745272339376271 1105 7 0.1149225568248724 1105 8 0.11898531712018467 1105 9 0.1417053757147002 1105 10-11 0.1697305930516677 1105 12-13 0.19758693085704238 1105 14-15 0.19975630451377668 1105 16-17 0.25991723693103097 1105 18-19 0.18319108098330616 1105 20-21 0.1538892868201458 1105 22-23 0.15899364332262422 1105 24-25 0.2386796190499325 1105 26-27 0.1494173674666115 1105 28-29 0.17844334184253086 1105 30-31 0.22742454603613282 1105 32-33 0.13684051187222224 1105 34-35 0.06702893783066344 1105 36-37 0.052682732863949866 1105 38-39 0.08447297986170099 1105 40-41 0.12088480429775927 1105 42-43 0.04385602460969551 1105 44-45 0.12222070179741706 1105 46-47 0.027081482498253706 1105 48-49 -0.03437562672318961 1105 50-51 0.015508290584016038 1105 52-53 -0.03469923302522915 1105 54-55 -0.13919134650861054 1105 56-57 -0.07798070435553939 1105 58-59 -0.19769272737985943 1105 60-61 -0.21974883933661005 1105 62-63 -0.11810585370485427 1105 64-65 -0.03330939321300974 1105 66-67 -0.07169682822117096 1105 68-69 -0.12438927008349054 1105 70-71 -0.03540806697808563 1105 72-73 0.03589655646207035 1105 74-75 0.029268034947783406 1105 76-77 0.048226432450974244 1105 78-79 0.11688341972548955 1105 80-81 0.09044935684995181 1105 82-83 0.03182153007917776 1105 84-85 0.051086758624819595 1105 86-87 0.00895995894993007 1105 88-89 0.0946923167974063 1105 90-91 0.12689964446365565 1105 92-93 0.1928836336504105 1105 94-95 0.03377834843424665 1105 96-97 0.04023456614422116 1105 98-99 0.03597786162040961 1105 100-101 0.00203033816143261 1105 102-103 -0.0015145071189230919 1105 104-105 0.019083438564393873 1105 106-107 0.04300593160120769 1105 108-109 0.11935890877160205 1105 110-111 0.12022907451570575 1105 112-113 0.1414615833561399 1105 114-115 0.02416693344441967 1105 116-117 0.04729043823856216 1105 118-119 7.383889394247944E-4 1105 120-121 0.030387443667784453 1105 122-123 0.05285899221229684 1105 124-125 -0.017055077125135654 1106 1 0.12791723746137507 1106 2 0.11883548967226432 1106 3 0.11313691024789563 1106 4 0.07333843546386021 1106 5 0.12043032552685418 1106 6 0.02832886168357618 1106 7 0.057157930729623274 1106 8 0.026283425814575878 1106 9 0.13307708114122363 1106 10-11 0.09055502586266329 1106 12-13 0.12488598971533804 1106 14-15 0.14781295781858717 1106 16-17 0.14978572204287843 1106 18-19 0.0874261641727685 1106 20-21 0.1129219131821344 1106 22-23 0.054127692707609754 1106 24-25 0.13509557247621728 1106 26-27 0.21317587154250361 1106 28-29 0.09991436554824418 1106 30-31 0.08361326209882236 1106 32-33 0.08800034382396404 1106 34-35 0.13188590547409262 1106 36-37 0.15210953995148202 1106 38-39 0.08457911055456435 1106 40-41 0.03096628303174498 1106 42-43 0.10215037797739512 1106 44-45 0.05347084072619168 1106 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-0.7447458157663931 2216 34-35 -0.7611007750099219 2216 36-37 -0.7235600552815313 2216 38-39 -0.7465234451466145 2216 40-41 -1.0317396436956727 2216 42-43 -0.9102433249699686 2216 44-45 -0.8199404183795451 2216 46-47 -0.9041326032396739 2216 48-49 -0.8105728332413165 2216 50-51 -0.7583554556360426 2216 52-53 -0.8692588071971201 2216 54-55 -0.7313527567065208 2216 56-57 -0.7540284534061996 2216 58-59 -0.6496063823399965 2216 60-61 -0.8069038594060558 2216 62-63 -0.7941926105744841 2216 64-65 -0.6506373237781347 2216 66-67 -0.6268430567886227 2216 68-69 -0.7727724515747312 2216 70-71 -0.7059974765727475 2216 72-73 -0.702960596614858 2216 74-75 -0.8432213019482973 2216 76-77 -0.7761473639620462 2216 78-79 -0.6750502756828993 2216 80-81 -0.7262943748303883 2216 82-83 -0.6704660102337385 2216 84-85 -0.6961977751374846 2216 86-87 -0.6290297414390835 2216 88-89 -0.5309219475934341 2216 90-91 -0.5084520736205036 2216 92-93 -0.48305540587751494 2216 94-95 -0.6007530937046397 2216 96-97 -0.5601017131575645 2216 98-99 -0.6013109064478996 2216 100-101 -0.6965504926132695 2216 102-103 -0.6294859651896267 2216 104-105 -0.6509014223415619 2216 106-107 -0.5806503046228002 2216 108-109 -0.480187788469717 2216 110-111 -0.4983932679255787 2216 112-113 -0.41440737744076017 2216 114-115 -0.3976408541983716 2216 116-117 -0.5015104217702593 2216 118-119 -0.5377651319150161 2216 120-121 -0.41357910531482034 2216 122-123 -0.39357524843549285 2216 124-125 -0.3701945540046623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 271.0 3 16.0 4 48.0 5 115.0 6 299.0 7 560.0 8 1093.0 9 1755.0 10 2907.0 11 4037.0 12 5117.0 13 7033.0 14 10358.0 15 8917.0 16 4757.0 17 4273.0 18 4536.0 19 4242.0 20 4106.0 21 4344.0 22 4728.0 23 5334.0 24 6816.0 25 9365.0 26 12365.0 27 16690.0 28 21755.0 29 27477.0 30 34771.0 31 44041.0 32 55893.0 33 73831.0 34 100918.0 35 152745.0 36 311328.0 37 693920.0 38 344164.0 39 1262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76061032904043 18.508090163434947 12.603018709431465 25.12828079809315 2 16.1214427443136 20.399791157630172 38.09178088468004 25.386985213376185 3 18.58747439188757 24.52301822537354 30.012682592323888 26.876824790415 4 12.763249381855788 16.389796126950788 36.82885851130835 34.018095979885075 5 13.093730384466967 38.23460958351343 34.88005747004837 13.791602561971231 6 32.778018370060025 36.805835314023284 16.7924908351126 13.623655480804093 7 28.53809837643686 32.43304885189562 21.273575952314662 17.75527681935286 8 26.730413601539027 35.074189892492505 19.7241750147393 18.471221491229176 9 26.625690330265982 14.970795801200994 19.49474042474349 38.908773443789535 10-11 25.032335827391883 26.108417787449017 27.96426016281448 20.89498622234462 12-13 25.717543212195025 23.425060178120187 28.256579063300684 22.600817546384103 14-15 22.580980542114112 25.106523202498053 25.458378289657517 26.854117965730318 16-17 22.44788632691685 28.516524375600085 26.39607952322717 22.6395097742559 18-19 22.58145884551656 26.839013647758243 28.331723045211753 22.247804461513443 20-21 22.90295003028422 26.306161114056458 28.47287989072507 22.31800896493425 22-23 22.883847291317483 26.15521599929916 28.275006331226614 22.68593037815674 24-25 22.762456669444187 26.46538464443141 28.26783507075121 22.50432361537319 26-27 22.47955367500161 26.70221571502223 28.114502440871302 22.703728169104853 28-29 22.513457389109178 26.525084754552275 28.402536229786225 22.558921626552323 30-31 22.490358376380794 26.259780080355256 28.308234903180978 22.941626640082973 32-33 22.274405001356875 26.578871714108633 28.30172577423709 22.8449975102974 34-35 22.918284617133235 26.480322407757 27.89275697752546 22.70863599758431 36-37 22.998891846538115 26.178552170566014 27.829152038554273 22.993403944341594 38-39 22.62904314373298 26.378263068448003 28.144512109008897 22.84818167881012 40-41 22.758935797692306 26.188806919807217 28.2188318545753 22.833425427925178 42-43 22.761577837333544 26.10947508970706 28.003808302038024 23.125138770921367 44-45 22.656634689066763 26.368026696445746 27.959069209166127 23.016269405321367 46-47 23.09377213726603 26.655622053713973 27.381409706135422 22.869196102884572 48-49 23.00609713117643 26.593485988178312 27.288538803129626 23.111878077515634 50-51 23.002290318081833 26.62478407118766 27.401523622901568 22.97140198782894 52-53 23.21626815601955 26.492243680982707 27.191926037175758 23.09956212582199 54-55 22.744711348931396 26.631555840411803 27.335240840867453 23.288491969789348 56-57 22.850458831765714 26.902597969827085 27.294001491804266 22.95294170660293 58-59 22.787694606648117 26.353851049196802 27.402141591975777 23.456312752179304 60-61 22.639238560930707 26.50262198439069 27.763228679683404 23.0949107749952 62-63 22.59120113060855 26.702067831478104 27.662501063595723 23.044229974317627 64-65 22.469561023206776 26.463243390476325 27.52968376089462 23.53751182542228 66-67 22.40121398438314 26.777664942928336 27.380604142510244 23.440516930178276 68-69 22.45634218363345 26.298305545555927 27.74799427042295 23.497358000387678 70-71 22.45271149144931 26.348143212202483 27.550397483014557 23.64874781333365 72-73 22.445528712344878 26.550007451474787 27.259005207976706 23.74545862820363 74-75 22.51676201686951 26.71888397215368 27.25048044318083 23.513873567795983 76-77 22.661134122819252 26.50508235125897 27.333780756796816 23.50000276912496 78-79 22.458610392677024 26.54531018479126 27.303370729946373 23.69270869258534 80-81 22.537813755234918 26.828432641117484 27.104792007983157 23.52896159566445 82-83 22.45518674727002 26.7136981563166 27.12607121081751 23.705043885595867 84-85 22.294443293438604 26.54105067225594 27.323278716917425 23.84122731738803 86-87 22.55354103113151 26.47220528580642 27.27756752007742 23.696686162984655 88-89 22.41125835583457 26.605551239636547 27.082470079604793 23.900720324924087 90-91 22.317334671911556 26.81703686510887 27.142434221953927 23.723194241025645 92-93 22.324282658178713 26.259939270572207 27.316108704769494 24.099669366479592 94-95 22.096509543159833 26.244130084428104 27.429391089560045 24.229969282852018 96-97 22.332741076243074 26.14283045856206 27.299267390230632 24.22516107496424 98-99 22.189652763530038 26.145256037650118 27.12250762455112 24.54258357426873 100-101 21.970237444913295 26.003065169593796 27.424608055535554 24.602089329957348 102-103 21.95213743721009 25.771440453492044 27.490513229620372 24.785908879677493 104-105 21.887635075176377 25.89793131226675 27.41419697532302 24.800236637233862 106-107 21.96618998266276 25.9333652705826 27.229693686871805 24.870751059882846 108-109 21.939658405998713 25.818577465348945 27.10873097269655 25.133033155955793 110-111 21.88964785606807 26.021225962064953 27.272141384231603 24.81698479763537 112-113 21.684111314795636 26.01129702288858 27.467504318576246 24.837087343739537 114-115 21.612139239658703 26.088807347948606 27.134554802745157 25.164498609647527 116-117 21.389557325054824 26.074716548521497 27.413815369301926 25.12191075712175 118-119 21.264739926300997 26.158287210620145 27.074263400173297 25.50270946290556 120-121 21.184613641798936 26.356187143630343 27.218145430286857 25.241053784283864 122-123 21.210243836126814 26.42807513959027 27.137958986390892 25.22372203789202 124-125 21.093931330666578 26.34966027837286 27.132115084949287 25.424293306011275 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 93.0 1 76.0 2 128.5 3 182.0 4 117.0 5 100.0 6 249.0 7 595.0 8 997.5 9 1196.0 10 990.5 11 672.0 12 618.0 13 728.0 14 842.0 15 944.5 16 1035.5 17 1138.0 18 1312.0 19 1466.0 20 1646.5 21 1990.0 22 2404.5 23 3049.5 24 4069.0 25 5314.0 26 7166.5 27 9564.5 28 11927.0 29 15131.0 30 19638.0 31 24093.0 32 29583.5 33 33734.5 34 36420.5 35 39843.0 36 45018.5 37 50222.0 38 53593.5 39 56529.0 40 57955.0 41 61129.5 42 65183.5 43 69430.5 44 73850.5 45 77257.0 46 76939.0 47 75194.5 48 76143.5 49 74974.0 50 73304.0 51 72009.0 52 71567.0 53 72591.0 54 73913.0 55 70156.0 56 61465.0 57 55579.0 58 52118.0 59 46253.5 60 37722.0 61 30616.0 62 24485.0 63 18293.5 64 13460.0 65 10128.5 66 7621.0 67 6364.5 68 4981.5 69 3243.0 70 2216.0 71 1600.5 72 1208.0 73 904.5 74 708.0 75 623.0 76 365.5 77 136.0 78 62.0 79 28.0 80 11.5 81 4.5 82 4.0 83 5.0 84 4.0 85 3.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02537525419308454 2 0.0 3 0.0 4 0.0 5 0.058050928739338245 6 0.01747066112103241 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.0139090629432173E-4 22-23 0.0 24-25 1.0069545314716086E-4 26-27 5.538249923093848E-4 28-29 5.286511290225946E-4 30-31 3.52434086015063E-4 32-33 3.0208635944148257E-4 34-35 3.272602227282728E-4 36-37 0.0 38-39 2.5173863286790216E-5 40-41 2.2656476958111195E-4 42-43 0.0 44-45 2.517386328679022E-4 46-47 0.0 48-49 3.52434086015063E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 7.552158986037064E-5 58-59 5.034772657358043E-5 60-61 2.5173863286790216E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.0069545314716086E-4 70-71 7.552158986037064E-5 72-73 1.5104317972074128E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.517386328679022E-4 82-83 0.0 84-85 7.552158986037064E-5 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.2656476958111195E-4 100-101 0.0 102-103 0.0 104-105 0.0012335193010527205 106-107 2.5173863286790216E-5 108-109 0.001409736344060252 110-111 0.004103339715746805 112-113 0.0 114-115 0.0 116-117 2.5173863286790216E-5 118-119 0.0 120-121 6.041727188829651E-4 122-123 8.559113517508674E-4 124-125 1.0069545314716086E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1986187.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.86117755084245 #Duplication Level Percentage of deduplicated Percentage of total 1 83.05432732498693 53.03947143666776 2 10.271803837866104 13.119389773147844 3 3.05444361345177 5.851810977530407 4 1.2685638045549243 3.240479134290169 5 0.613074054399823 1.9575815519920972 6 0.36717635884790784 1.406898878691485 7 0.24179726645996905 1.0809020715295912 8 0.16318627041524078 0.8337013911077985 9 0.10872074366298135 0.6248731243069153 >10 0.7280000842388022 8.978199423407899 >50 0.08382809924436722 3.7208100152114087 >100 0.0432681586986777 4.75505541151718 >500 0.0012672682207446487 0.5269649711315512 >1k 5.431149517477066E-4 0.8638618394679575 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2988 0.15043900700185833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2898 0.1459077116102361 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.034772657358043E-5 0.0 10-11 0.0 0.0 0.0 5.034772657358043E-5 0.0 12-13 0.0 0.0 0.0 5.034772657358043E-5 0.0 14-15 0.0 0.0 0.0 5.034772657358043E-5 0.0 16-17 0.0 0.0 0.0 5.034772657358043E-5 0.0 18-19 0.0 0.0 0.0 5.034772657358043E-5 0.0 20-21 0.0 0.0 0.0 1.0069545314716086E-4 0.0 22-23 0.0 0.0 0.0 2.0139090629432173E-4 0.0 24-25 0.0 0.0 0.0 4.0278181258864346E-4 0.0 26-27 0.0 0.0 0.0 5.789988555961749E-4 0.0 28-29 0.0 0.0 0.0 0.002945342004554455 0.0 30-31 0.0 0.0 0.0 0.011076499846187696 0.0 32-33 0.0 0.0 0.0 0.03003241890114073 0.0 34-35 0.0 0.0 0.0 0.05500489128163663 0.0 36-37 0.0 0.0 0.0 0.09946193384610816 0.0 38-39 0.0 0.0 0.0 0.1711570964868867 0.0 40-41 0.0 0.0 0.0 0.2498254192581061 0.0 42-43 0.0 0.0 0.0 0.3219233637114733 0.0 44-45 0.0 0.0 0.0 0.41315344426280104 0.0 46-47 0.0 0.0 0.0 0.5263351336002098 0.0 48-49 0.0 0.0 0.0 0.65064367051038 0.0 50-51 0.0 0.0 0.0 0.7877153561069526 0.0 52-53 0.0 0.0 0.0 0.9166055361353186 0.0 54-55 0.0 0.0 0.0 1.0510591399500653 0.0 56-57 0.0 0.0 0.0 1.2162248569746956 0.0 58-59 0.0 0.0 0.0 1.3967718044675552 0.0 60-61 0.0 0.0 0.0 1.5971305823671185 0.0 62-63 0.0 0.0 0.0 1.7955006250670253 0.0 64-65 0.0 0.0 0.0 1.9975712256700904 0.0 66-67 0.0 0.0 0.0 2.233928628069764 0.0 68-69 0.0 0.0 0.0 2.4980779755380533 0.0 70-71 0.0 0.0 0.0 2.7833230204406734 0.0 72-73 0.0 0.0 0.0 3.070028149413927 0.0 74-75 0.0 0.0 0.0 3.3622211805837017 0.0 76-77 0.0 0.0 0.0 3.67296734899584 0.0 78-79 0.0 0.0 0.0 4.034438851930861 0.0 80-81 0.0 0.0 0.0 4.4248854715089765 0.0 82-83 0.0 0.0 0.0 4.821323470549349 0.0 84-85 0.0 0.0 0.0 5.209504442431654 0.0 86-87 0.0 0.0 0.0 5.6073270039528005 0.0 88-89 0.0 0.0 0.0 6.0453270512796635 0.0 90-91 0.0 0.0 0.0 6.526324057100363 0.0 92-93 0.0 0.0 0.0 7.028769194441409 0.0 94-95 0.0 0.0 0.0 7.532321981767074 0.0 96-97 0.0 0.0 0.0 8.029228869185026 0.0 98-99 0.0 0.0 0.0 8.552669008507255 0.0 100-101 0.0 0.0 0.0 9.140126282167792 0.0 102-103 0.0 0.0 0.0 9.748553383946224 0.0 104-105 0.0 0.0 0.0 10.364129862898107 0.0 106-107 0.0 0.0 0.0 10.95254374336354 0.0 108-109 0.0 0.0 0.0 11.565225228037441 0.0 110-111 0.0 0.0 0.0 12.237241508478306 0.0 112-113 0.0 0.0 0.0 12.94213485437172 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2855 0.0 66.53414 1 GTATCAA 4465 0.0 47.744305 1 ATCAACG 5425 0.0 38.62917 3 TCAACGC 5515 0.0 37.890823 4 GTACATG 6770 0.0 37.821587 1 CAACGCA 5655 0.0 36.952766 5 AACGCAG 5845 0.0 35.83627 6 TATCAAC 5860 0.0 35.660053 2 TACATGG 7590 0.0 33.179592 2 ACATGGG 7260 0.0 33.047676 3 ACGCAGA 6900 0.0 30.266142 7 CGCAGAG 6935 0.0 30.113394 8 GTGGTAT 1535 0.0 27.54292 1 CATGGGG 4375 0.0 27.488167 4 GAGTACA 5340 0.0 26.093636 1 CATGGGA 4095 0.0 25.733084 4 TGGTATC 1625 0.0 25.279482 2 GCAGAGT 8230 0.0 24.941256 9 ATGGGGA 2665 0.0 24.796947 5 CAGAGTA 7990 0.0 23.866037 10-11 >>END_MODULE