FastQCFastQC Report
Thu 26 May 2016
SRR1364336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003293
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.056.4153561
GTATCAA33700.047.5336151
ATCAACG37950.039.5240173
GTACATG107150.039.2365651
TCAACGC37850.039.1566734
CAACGCA38850.038.1459245
TACATGG109600.037.743882
ACATGGG110000.036.740873
AACGCAG40850.036.4167376
CATGGGA76200.034.2911384
TATCAAC45200.033.8428572
ACGCAGA50750.029.1882427
CATGGGG37400.028.4874634
CGCAGAG52300.028.3231988
ATGGGAT36400.027.1422925
GAGTACA75700.025.4088551
ATGGGAG32950.024.2041135
AGTACAT76500.023.730712
CCTATAC6400.023.2505043
GCAGAGT66700.021.494929
Produced by FastQC (version 0.11.5)