##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364336_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2003293 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.395615618883507 33.0 31.0 34.0 28.0 34.0 2 31.361711442110565 33.0 31.0 34.0 28.0 34.0 3 31.512901507667625 33.0 31.0 34.0 28.0 34.0 4 34.96302238364533 37.0 35.0 37.0 32.0 37.0 5 35.027596063082136 37.0 35.0 37.0 32.0 37.0 6 35.094645166732974 37.0 35.0 37.0 32.0 37.0 7 35.00772178607922 37.0 35.0 37.0 32.0 37.0 8 34.933587348430805 37.0 35.0 37.0 32.0 37.0 9 36.52727134772597 39.0 37.0 39.0 32.0 39.0 10-11 36.46343146010094 39.0 37.0 39.0 32.0 39.0 12-13 36.507401313736935 39.0 37.0 39.0 32.0 39.0 14-15 37.85339388696511 40.0 38.0 41.0 32.0 41.0 16-17 37.8660283343475 40.0 38.0 41.0 32.5 41.0 18-19 37.875147070348675 40.0 38.0 41.0 32.0 41.0 20-21 37.910358844163085 40.0 38.0 41.0 32.0 41.0 22-23 37.86653774560187 40.0 38.0 41.0 32.0 41.0 24-25 37.87784987018874 40.0 38.0 41.0 32.0 41.0 26-27 37.840555275738495 40.0 38.0 41.0 32.0 41.0 28-29 37.85571331802188 40.0 38.0 41.0 32.0 41.0 30-31 37.773761252098424 40.0 38.0 41.0 32.0 41.0 32-33 37.74523771610044 40.0 38.0 41.0 32.0 41.0 34-35 37.74014460191295 40.0 38.0 41.0 32.0 41.0 36-37 37.68433000065392 40.0 38.0 41.0 32.0 41.0 38-39 37.60292802900025 40.0 38.0 41.0 31.0 41.0 40-41 37.55342703239117 40.0 38.0 41.0 31.0 41.0 42-43 37.43749142037635 40.0 38.0 41.0 31.0 41.0 44-45 37.320576670512 40.0 37.0 41.0 31.0 41.0 46-47 37.22671371586682 40.0 37.0 41.0 31.0 41.0 48-49 37.101864030873166 40.0 37.0 41.0 31.0 41.0 50-51 36.998790241866764 40.0 37.0 41.0 30.0 41.0 52-53 36.85978161956339 40.0 36.0 41.0 30.0 41.0 54-55 36.693935435305775 40.0 36.0 41.0 30.0 41.0 56-57 36.52173171872512 39.5 35.5 41.0 30.0 41.0 58-59 36.32901228127887 39.0 35.0 41.0 30.0 41.0 60-61 36.09933394665683 39.0 35.0 41.0 30.0 41.0 62-63 35.8480981064677 38.5 35.0 40.5 29.0 41.0 64-65 35.53007947414582 38.0 35.0 40.0 29.0 41.0 66-67 35.22661038599945 37.5 34.5 40.0 29.0 41.0 68-69 34.89953516535025 37.0 34.0 40.0 28.0 41.0 70-71 34.54177272121452 36.5 34.0 39.0 28.0 41.0 72-73 34.16696783745563 36.0 34.0 39.0 28.0 40.5 74-75 33.7996306581214 35.5 34.0 38.5 27.5 40.0 76-77 33.54536256054406 35.0 33.5 37.0 27.5 39.0 78-79 33.372534621745295 35.0 34.0 37.0 28.0 39.0 80-81 33.082558068140806 35.0 34.0 36.5 27.5 39.0 82-83 32.777586204314595 35.0 33.5 36.0 27.0 37.5 84-85 32.510277827556926 35.0 33.0 36.0 27.0 37.0 86-87 32.28694279868197 35.0 33.0 35.5 26.5 37.0 88-89 32.01587760751922 35.0 33.0 35.0 26.0 36.0 90-91 31.884912241993558 35.0 33.0 35.0 26.0 36.0 92-93 31.731606410045856 35.0 33.0 35.0 25.5 36.0 94-95 31.627164873036545 35.0 33.0 35.0 25.0 36.0 96-97 31.516448916858394 35.0 33.0 35.0 25.0 35.0 98-99 31.38815365500703 35.0 33.0 35.0 25.0 35.0 100-101 31.30168652313965 35.0 33.0 35.0 24.5 35.0 102-103 30.29113514598214 34.0 30.5 35.0 21.5 35.0 104-105 30.981897805263632 34.5 32.0 35.0 23.5 35.0 106-107 31.30466511888176 35.0 33.0 35.0 24.5 35.0 108-109 31.331291528498326 35.0 33.0 35.0 24.5 35.0 110-111 31.26225669435275 35.0 33.0 35.0 24.0 35.0 112-113 31.166972829236663 35.0 33.0 35.0 24.0 35.0 114-115 31.062134944813366 35.0 33.0 35.0 23.0 35.0 116-117 30.929792596489882 35.0 33.0 35.0 20.0 35.0 118-119 30.75378189810477 35.0 33.0 35.0 18.5 35.0 120-121 30.5616724563007 35.0 33.0 35.0 12.0 35.0 122-123 30.368282872250838 35.0 32.5 35.0 2.0 35.0 124-125 29.55076391721031 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26315782295975865 1101 2 0.22498532626814338 1101 3 0.2428832005426962 1101 4 0.3073376580670555 1101 5 0.16737563977991954 1101 6 0.22490555394059442 1101 7 0.22071742945529849 1101 8 0.14717335962499334 1101 9 0.28809241094139537 1101 10-11 0.33712785029713643 1101 12-13 0.4272885332791958 1101 14-15 0.4789184395621646 1101 16-17 0.4586789384455585 1101 18-19 0.40104605724189213 1101 20-21 0.45557529317503054 1101 22-23 0.5541720036734503 1101 24-25 0.5232773156824848 1101 26-27 0.587365678629638 1101 28-29 0.6131383826656958 1101 30-31 0.6042410489223826 1101 32-33 0.5568494924420406 1101 34-35 0.48917469704992556 1101 36-37 0.38742603326583236 1101 38-39 0.5206881486886559 1101 40-41 0.4354020607089666 1101 42-43 0.530712270397288 1101 44-45 0.511514525209634 1101 46-47 0.47701554346750896 1101 48-49 0.43327748035423497 1101 50-51 0.3639364436495285 1101 52-53 0.45645968521296965 1101 54-55 0.4273504941382953 1101 56-57 0.32609808817191066 1101 58-59 0.33674030820171197 1101 60-61 0.26194071332665914 1101 62-63 0.27920939146356716 1101 64-65 0.27311914290830686 1101 66-67 0.2366382484089513 1101 68-69 0.23161705116509523 1101 70-71 0.23641629298560218 1101 72-73 0.3422154422430239 1101 74-75 0.3064197859558391 1101 76-77 0.3399679361935597 1101 78-79 0.360582838806657 1101 80-81 0.47051211262986214 1101 82-83 0.3008501629697591 1101 84-85 0.26369549141834625 1101 86-87 0.2412410095855293 1101 88-89 0.2894902219959832 1101 90-91 0.22915415685494978 1101 92-93 0.29983453433606755 1101 94-95 0.26979531283596714 1101 96-97 0.1703962862076871 1101 98-99 0.11366511569839233 1101 100-101 0.07448167416202267 1101 102-103 -0.1847286622383919 1101 104-105 0.029913789008823244 1101 106-107 0.13679410319599938 1101 108-109 0.15644856809769792 1101 110-111 0.13511077670973037 1101 112-113 0.17616575748169794 1101 114-115 0.10230347821467234 1101 116-117 0.1687284210643405 1101 118-119 0.18495888175106856 1101 120-121 0.07487210194326366 1101 122-123 0.00894692015073062 1101 124-125 -0.024305866704303014 1102 1 0.1966685490970086 1102 2 0.2794421848605637 1102 3 0.3490970916649019 1102 4 0.34713954064065433 1102 5 0.2647233059696532 1102 6 0.23689300827148685 1102 7 0.1937092846545525 1102 8 0.29428266051252194 1102 9 0.37612641250116496 1102 10-11 0.41522891959604635 1102 12-13 0.2926558328142761 1102 14-15 0.40277027819940514 1102 16-17 0.47596913177759603 1102 18-19 0.4417034633211401 1102 20-21 0.4439428856191938 1102 22-23 0.5318050211551721 1102 24-25 0.4766346804169572 1102 26-27 0.5131830817677496 1102 28-29 0.42057845383175163 1102 30-31 0.48699292360070245 1102 32-33 0.5385286931257411 1102 34-35 0.44891735895451035 1102 36-37 0.3046782569037205 1102 38-39 0.40367344658200466 1102 40-41 0.3399989250149247 1102 42-43 0.222438432963024 1102 44-45 0.3031411830689379 1102 46-47 0.3475717331906196 1102 48-49 0.4083228330444797 1102 50-51 0.2838056832551743 1102 52-53 0.25224565185508396 1102 54-55 0.2968246681166349 1102 56-57 0.27105492928622255 1102 58-59 0.276215952752338 1102 60-61 0.26039510569704305 1102 62-63 0.26002197908014324 1102 64-65 0.2010003495972441 1102 66-67 0.17443647624881464 1102 68-69 0.025812871123655157 1102 70-71 0.09391241465255717 1102 72-73 0.11346647736920801 1102 74-75 0.12214797839348535 1102 76-77 0.24004713012708123 1102 78-79 0.31639599898210946 1102 80-81 0.2585466566138379 1102 82-83 0.25119870065437766 1102 84-85 0.18873566094399052 1102 86-87 0.1234031486172924 1102 88-89 0.2560823756490862 1102 90-91 0.19217480875241577 1102 92-93 0.15400354648516057 1102 94-95 0.113437016337258 1102 96-97 0.12770129374738204 1102 98-99 0.1397599386812658 1102 100-101 0.18676004867338847 1102 102-103 -0.044492960841708395 1102 104-105 0.12718382826828645 1102 106-107 0.17162653630605362 1102 108-109 0.18900426602286302 1102 110-111 0.18036378143846576 1102 112-113 0.1541295331880832 1102 114-115 0.1560813422445939 1102 116-117 0.09888979829144162 1102 118-119 0.10918577658948081 1102 120-121 -0.012863566305274077 1102 122-123 -0.09951278797683116 1102 124-125 -0.09356216696141573 1103 1 0.32592282579913956 1103 2 0.34969554808662906 1103 3 0.36532745692600344 1103 4 0.3896385148905068 1103 5 0.38367093922018825 1103 6 0.30816154084331515 1103 7 0.1921169912418108 1103 8 0.35331167190098967 1103 9 0.4354813126477737 1103 10-11 0.5234369781713752 1103 12-13 0.5390624742672827 1103 14-15 0.704566957472899 1103 16-17 0.5413907709322103 1103 18-19 0.5540974642404919 1103 20-21 0.6505375479563469 1103 22-23 0.6113762517144181 1103 24-25 0.6398682093384096 1103 26-27 0.6973156968604428 1103 28-29 0.6147327948416503 1103 30-31 0.7318868627986959 1103 32-33 0.6948054610898851 1103 34-35 0.6366490932540714 1103 36-37 0.6084524296318179 1103 38-39 0.5731086785426172 1103 40-41 0.5825337564333353 1103 42-43 0.4871395781586969 1103 44-45 0.5397828594444718 1103 46-47 0.5712449297807822 1103 48-49 0.5470789655020383 1103 50-51 0.38783685195198103 1103 52-53 0.38147065499993715 1103 54-55 0.4141701325375067 1103 56-57 0.340970999998909 1103 58-59 0.38299319273789934 1103 60-61 0.2824337444413558 1103 62-63 0.28094745847872815 1103 64-65 0.3376372646110539 1103 66-67 0.2845099893225722 1103 68-69 0.36752669094666857 1103 70-71 0.36430955924630126 1103 72-73 0.3280264863981657 1103 74-75 0.2628796057093581 1103 76-77 0.467795817225678 1103 78-79 0.470212553707789 1103 80-81 0.43259747033643237 1103 82-83 0.315048149923534 1103 84-85 0.21248874260311368 1103 86-87 0.3318191787702389 1103 88-89 0.39644684907187155 1103 90-91 0.25906482835040734 1103 92-93 0.20035699000777285 1103 94-95 0.17998057339189089 1103 96-97 0.2587691790091462 1103 98-99 0.27591284070184585 1103 100-101 0.19821882409817704 1103 102-103 -0.03843445134778989 1103 104-105 0.17296161728000214 1103 106-107 0.2972207953970205 1103 108-109 0.21704839267743736 1103 110-111 0.18089705395177091 1103 112-113 0.09773719933316016 1103 114-115 0.06535588706429252 1103 116-117 0.05890170757398394 1103 118-119 0.0016873372580441526 1103 120-121 -0.15481898114920156 1103 122-123 -0.11010188010859068 1103 124-125 -0.17476137175522055 1104 1 0.4758993286745259 1104 2 0.3551312006675609 1104 3 0.4787122416790268 1104 4 0.49513546330222624 1104 5 0.33684576179994963 1104 6 0.32205136772173404 1104 7 0.40650237259511357 1104 8 0.41786940834387565 1104 9 0.519046832469364 1104 10-11 0.5161517595336136 1104 12-13 0.4384408578941219 1104 14-15 0.5510335788200678 1104 16-17 0.525202700928304 1104 18-19 0.5183465482499017 1104 20-21 0.5890364240436554 1104 22-23 0.5517958512374221 1104 24-25 0.5785414735832575 1104 26-27 0.5966450064328939 1104 28-29 0.5948894672655598 1104 30-31 0.6198272527785207 1104 32-33 0.593453225521344 1104 34-35 0.5731271114661567 1104 36-37 0.5378791488653576 1104 38-39 0.4403158063386954 1104 40-41 0.5475679084698939 1104 42-43 0.4552674987952017 1104 44-45 0.3889313194422215 1104 46-47 0.46726840073255715 1104 48-49 0.5172019217137205 1104 50-51 0.3550220833723685 1104 52-53 0.3415642181899088 1104 54-55 0.307218255256295 1104 56-57 0.2286378021255473 1104 58-59 0.24926604401289865 1104 60-61 0.15823093492837614 1104 62-63 0.2117312505804705 1104 64-65 0.2719990226493181 1104 66-67 0.20995201218313753 1104 68-69 0.3583139135584972 1104 70-71 0.1398084963256494 1104 72-73 0.0911400668949156 1104 74-75 0.04609251676589565 1104 76-77 0.1019373496027498 1104 78-79 0.2521342329191114 1104 80-81 0.19727207313453476 1104 82-83 0.17273847488024074 1104 84-85 0.11368245729170212 1104 86-87 0.15202498834374012 1104 88-89 0.21789662750251892 1104 90-91 0.14754830453208356 1104 92-93 -0.008950395146673173 1104 94-95 -0.033155347483909026 1104 96-97 0.02754327641460108 1104 98-99 -0.012382915537511252 1104 100-101 0.00831485267516996 1104 102-103 -0.1603905454923904 1104 104-105 -0.1750740885345934 1104 106-107 -0.13900957410490378 1104 108-109 -0.06636683201046267 1104 110-111 -0.05477548376339314 1104 112-113 -0.10629221285081059 1104 114-115 -0.14716491390840503 1104 116-117 -0.11679772038612768 1104 118-119 -0.2302559812743965 1104 120-121 -0.17902633585436334 1104 122-123 -0.2684162400882819 1104 124-125 -0.40864422544420265 1105 1 0.1095531013312403 1105 2 0.060926368367077544 1105 3 0.0014076000609932748 1105 4 0.21226221968765202 1105 5 0.18297223328376333 1105 6 0.21216899529437683 1105 7 0.167713923265282 1105 8 0.23194154324576033 1105 9 0.2545552679380094 1105 10-11 0.3425690425473107 1105 12-13 0.374138984754282 1105 14-15 0.4365860025969255 1105 16-17 0.360168570261564 1105 18-19 0.31460959821908574 1105 20-21 0.38060304004469003 1105 22-23 0.32128384510058794 1105 24-25 0.33102872392269234 1105 26-27 0.35173077366633976 1105 28-29 0.3888716525418374 1105 30-31 0.29859111239914427 1105 32-33 0.41303782506825826 1105 34-35 0.30669591486455516 1105 36-37 0.30442397575943403 1105 38-39 0.27828493284583544 1105 40-41 0.3657699206886491 1105 42-43 0.2965602116816939 1105 44-45 0.22831014506660097 1105 46-47 0.28647520091605116 1105 48-49 0.14726378101971704 1105 50-51 0.23624026617850546 1105 52-53 0.30822378035274056 1105 54-55 0.2685138410811092 1105 56-57 0.19634475649309735 1105 58-59 0.17447042760188936 1105 60-61 0.24536919331944773 1105 62-63 0.1888807398592931 1105 64-65 0.15986084684669066 1105 66-67 0.14572190554380882 1105 68-69 0.09585766976473309 1105 70-71 0.08923168162139206 1105 72-73 0.08915600794340861 1105 74-75 0.045979285176201756 1105 76-77 0.08362794963790066 1105 78-79 0.19686855959953675 1105 80-81 0.17882301328139505 1105 82-83 0.14683185038797575 1105 84-85 0.17373358921459214 1105 86-87 0.12728132290504135 1105 88-89 0.11514414298613929 1105 90-91 0.09455102220415412 1105 92-93 0.10480309649292963 1105 94-95 0.112610616945652 1105 96-97 0.04813507892595226 1105 98-99 0.06680583594068068 1105 100-101 -0.04416064162799671 1105 102-103 -0.11744752205629538 1105 104-105 0.023631972852648175 1105 106-107 0.0644047684188358 1105 108-109 0.14328151327617178 1105 110-111 0.19605316300861375 1105 112-113 0.04575212426537689 1105 114-115 0.03775229659834878 1105 116-117 -0.07779462087070854 1105 118-119 -0.06294639688103487 1105 120-121 -0.03938325002698306 1105 122-123 -0.12753592257505275 1105 124-125 -0.16085961744478894 1106 1 0.307803525099434 1106 2 0.2772660630761301 1106 3 0.30817900966728473 1106 4 0.41767866635054673 1106 5 0.3064636689424063 1106 6 0.27634145092031304 1106 7 0.34341307978466773 1106 8 0.34664063610909324 1106 9 0.439720632149843 1106 10-11 0.5046138679014689 1106 12-13 0.362429741733159 1106 14-15 0.501946939449148 1106 16-17 0.5638147389210246 1106 18-19 0.45477338838055203 1106 20-21 0.4088866593043008 1106 22-23 0.44886108824706383 1106 24-25 0.39515275974229525 1106 26-27 0.4997274028236518 1106 28-29 0.4723509230630185 1106 30-31 0.5273137523619411 1106 32-33 0.4765456876748573 1106 34-35 0.45470544874068963 1106 36-37 0.43294440203157336 1106 38-39 0.4209181873519441 1106 40-41 0.4505108714714652 1106 42-43 0.36498504326697656 1106 44-45 0.34159064062515654 1106 46-47 0.4424124072049196 1106 48-49 0.29032061394362074 1106 50-51 0.2874246103037663 1106 52-53 0.34789001227135685 1106 54-55 0.38843289270790393 1106 56-57 0.3343466809228559 1106 58-59 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-1.0008394287120446 2216 108-109 -0.9818373055138458 2216 110-111 -0.9974406881733522 2216 112-113 -1.0076872002274584 2216 114-115 -1.016525643619591 2216 116-117 -1.0264803628284689 2216 118-119 -0.9362626677169565 2216 120-121 -0.8839994810360245 2216 122-123 -0.942258225260499 2216 124-125 -0.8733509481901152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23788.0 3 4701.0 4 3048.0 5 2492.0 6 2850.0 7 3374.0 8 3809.0 9 4048.0 10 4372.0 11 5124.0 12 5845.0 13 5938.0 14 5932.0 15 5565.0 16 4928.0 17 4565.0 18 4325.0 19 4330.0 20 4338.0 21 4470.0 22 5498.0 23 7489.0 24 11052.0 25 15992.0 26 19539.0 27 19867.0 28 23210.0 29 28813.0 30 34278.0 31 42926.0 32 56197.0 33 75478.0 34 107719.0 35 163704.0 36 316916.0 37 651557.0 38 314386.0 39 830.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.420478256136015 19.075360150382696 12.524283869318072 23.979877724163213 2 15.277530242192144 20.46379598607943 40.28852581450856 23.970147957219858 3 18.476114884174848 26.27934316243476 29.36794734516919 25.876594608221204 4 11.957425204314989 16.539392568830102 38.51899776203263 32.98418446482228 5 12.782206040367647 39.472845907113665 34.9150585782248 12.829889474293884 6 31.260620181127923 37.27288478947886 18.38173281409396 13.084762215299254 7 28.17089979128488 32.817045051215324 21.853436161678587 17.158618995821204 8 26.065072954989045 36.04320892132442 20.386514184167044 17.50520393951949 9 26.98836365923507 15.979739359145167 19.849168344321075 37.18272863729869 10-11 24.33959245530913 27.440018030344593 28.423175981304748 19.79721353304153 12-13 24.87256432060553 24.831306684011683 28.31893725519658 21.97719174018621 14-15 22.31938772504933 26.124192138604478 25.52594915446295 26.03047098188324 16-17 22.297875569157625 29.344335903522683 26.27573298816771 22.08205553915198 18-19 22.314470381751637 28.568651000453254 27.523271694790374 21.593606923004735 20-21 23.22525961005205 27.724177142335144 27.249932985339637 21.800630262273167 22-23 22.889138034226647 27.743670047267177 26.990410289458406 22.376781629047773 24-25 22.61885805022031 27.814653173549754 27.227070628210654 22.339418148019284 26-27 22.45379105991538 28.08357443647756 27.068320544383944 22.39431395922312 28-29 22.406702592667195 27.846544013094494 27.34681469356949 22.399938700668827 30-31 22.53900151625765 27.827408696954382 27.200663908700072 22.432925878087893 32-33 22.294786387055858 27.85323302805967 27.180263212738883 22.671717372145594 34-35 22.710654435325463 27.879905294795655 27.03663362234428 22.372806647534603 36-37 22.73498163894934 27.601552346777638 26.969367133407328 22.694098880865695 38-39 22.41821127432347 27.82744754681869 27.182657098722522 22.571684080135316 40-41 22.61688984288228 27.522506894959502 27.409042692590884 22.451560569567334 42-43 22.496551882499936 27.425350689648457 27.269754855443633 22.808342572407973 44-45 22.34604856549608 27.66459661745256 27.29105427745415 22.69830053959721 46-47 22.55879885678145 27.69017888809492 27.018334575871762 22.73268767925187 48-49 22.34917707993788 27.590721876430457 27.21331827146603 22.846782772165632 50-51 22.474770290716336 27.547018833490654 27.232661422967087 22.745549452825923 52-53 22.612310403266665 27.5857412773647 27.049641460316032 22.752306859052595 54-55 22.42098584426949 27.58982177168025 27.304006505535845 22.68518587851441 56-57 22.38183450041232 27.716653388522495 27.25186842457315 22.649643686492034 58-59 22.387654022710798 27.291696201621747 27.431192706347 22.889457069320454 60-61 22.39412180790498 27.64523885708809 27.31278225740028 22.647857077606652 62-63 22.25210935579319 27.478419739651645 27.50654864341061 22.76292226114455 64-65 22.236834518942754 27.417765896030943 27.328586236580932 23.01681334844537 66-67 22.239577483420568 27.633303132183695 27.180285887576844 22.946833496818893 68-69 22.279225006190046 27.47157570626433 27.378250772757408 22.87094851478822 70-71 22.393066864611722 27.328198509471736 27.329671116852893 22.94906350906365 72-73 22.40857653602951 27.41198852055652 27.102239230284248 23.077195713129726 74-75 22.157629785081152 27.60371986544828 27.17980755719035 23.058842792280224 76-77 22.275044402579393 27.495604668785212 27.273317219614807 22.956033709020588 78-79 22.37803970978927 27.48161223301921 27.247665524846326 22.892682532345198 80-81 22.2885944417225 27.743465728065363 27.111447340079152 22.85649249013298 82-83 22.511796885412597 27.550775402177326 27.139101165436934 22.798326546973144 84-85 22.429926136099866 27.477051374271884 27.11162549000913 22.981396999619125 86-87 22.39505321749774 27.339938794199302 27.27591755662011 22.98909043168285 88-89 22.59172044857305 27.327431967219663 27.24341437893879 22.837433205268496 90-91 22.57726763675881 27.452970265521977 27.184510606477723 22.78525149124149 92-93 22.55750870330491 27.20567753993237 27.239222827568238 22.997590929194484 94-95 22.4163191508267 27.156977655488586 27.338805730242516 23.0878974634422 96-97 22.606042181758642 27.17893577692218 27.264145904497767 22.950876136821407 98-99 22.628257542403084 27.122824935580454 27.06417095215133 23.18474656986513 100-101 22.348172195933188 27.20302286592709 27.31434116663077 23.134463771508955 102-103 22.52850916219292 27.0439501222882 27.237659424552835 23.189881290966046 104-105 22.4106138251682 27.202244925057677 27.180280880199113 23.206860369575004 106-107 22.388752644289273 27.23502505444584 27.16036717399579 23.215855127269098 108-109 22.538757614807725 27.22473937995304 26.941275221808148 23.295227783431084 110-111 22.431575786725265 27.445936154456508 26.97233229993263 23.150155758885592 112-113 22.383161661270403 27.431588258609313 27.224673254001768 22.960576826118515 114-115 22.322549083230584 27.619569395426385 26.980676207924205 23.077205313418826 116-117 22.280133512674745 27.615898221669678 27.013029541164368 23.09093872449121 118-119 22.251144560472298 27.67558958536121 26.847091115853445 23.226174738313045 120-121 22.322556327164346 27.739100263101403 26.880625851834473 23.05771755789978 122-123 22.382350151508344 27.928229473988985 26.810437352422884 22.878983022079783 124-125 22.40624969580431 27.931541679988882 26.733528567688193 22.928680056518623 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 798.0 1 652.5 2 459.5 3 345.5 4 253.5 5 199.5 6 170.5 7 171.5 8 183.5 9 191.5 10 168.0 11 149.5 12 154.0 13 159.5 14 183.5 15 207.0 16 247.0 17 318.0 18 414.0 19 539.0 20 722.5 21 1027.0 22 1589.0 23 2390.0 24 3291.0 25 4571.0 26 6852.5 27 10006.5 28 13076.5 29 16689.0 30 21223.5 31 25668.5 32 31103.0 33 36904.0 34 42409.5 35 47487.0 36 54507.0 37 61660.0 38 65697.5 39 69132.5 40 69922.0 41 71013.5 42 73225.5 43 75216.5 44 78001.0 45 80196.5 46 79797.0 47 78356.5 48 77727.5 49 76292.5 50 74837.0 51 71284.0 52 68088.0 53 65887.0 54 63908.0 55 59718.0 56 52707.5 57 47275.0 58 42892.5 59 37540.5 60 30567.0 61 24846.5 62 20406.5 63 15685.0 64 11699.0 65 9028.5 66 6709.0 67 5311.5 68 4074.0 69 2719.5 70 1971.5 71 1364.0 72 1016.0 73 703.0 74 497.5 75 402.5 76 270.0 77 169.5 78 104.0 79 74.5 80 46.5 81 32.5 82 25.5 83 20.5 84 17.0 85 13.0 86 9.5 87 7.5 88 7.5 89 6.0 90 4.5 91 3.0 92 3.0 93 4.5 94 4.0 95 2.5 96 2.0 97 2.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046673152654154934 2 0.010682411409614071 3 0.002895232998867365 4 0.006788822204240718 5 0.025008822972974996 6 0.030949042401685622 7 0.004193096067325149 8 1.497534309758982E-4 9 0.0 10-11 1.747123361385479E-4 12-13 0.0013727397839457334 14-15 4.991781032529939E-4 16-17 1.747123361385479E-4 18-19 4.991781032529939E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.991781032529939E-5 28-29 4.243013877650449E-4 30-31 2.7454795678914665E-4 32-33 4.243013877650449E-4 34-35 0.0011980274478071856 36-37 1.747123361385479E-4 38-39 3.2446576711444604E-4 40-41 8.985205858553891E-4 42-43 0.0012978630684577842 44-45 0.001971753507849326 46-47 7.48767154879491E-5 48-49 0.0 50-51 0.0 52-53 0.0013976986891083832 54-55 0.0037937535847227537 56-57 4.991781032529939E-5 58-59 8.486027755300898E-4 60-61 0.0011980274478071856 62-63 7.737260600421405E-4 64-65 0.0016722466458975298 66-67 0.002895232998867365 68-69 0.003843671395048053 70-71 0.0021714247491505237 72-73 0.0029950686195179634 74-75 0.0024210138007770207 76-77 0.001547452120084281 78-79 0.0029451508091926644 80-81 0.001422657594271033 82-83 4.991781032529939E-4 84-85 6.988493445541916E-4 86-87 0.0025707672317529188 88-89 0.007038411255867215 90-91 6.239726290662424E-4 92-93 0.001547452120084281 94-95 0.0013477808787830837 96-97 4.991781032529939E-4 98-99 9.484383961806884E-4 100-101 0.001422657594271033 102-103 0.0016722466458975298 104-105 9.484383961806884E-4 106-107 0.007762219505584056 108-109 0.002071589128499925 110-111 0.001822000076873428 112-113 0.002745479567891467 114-115 0.0031448220504938615 116-117 0.001871917887198727 118-119 0.0497181390839982 120-121 0.013128384115553742 122-123 0.0051415344635058376 124-125 0.002970109714355314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2003293.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.17913388765729 #Duplication Level Percentage of deduplicated Percentage of total 1 81.69564043527365 48.346772433569676 2 10.429478218051452 12.344149756889445 3 3.33750202468928 5.925314375082427 4 1.482040503249489 3.5082349347493 5 0.7947002432319525 2.3514836047388763 6 0.4729901824042963 1.6794689599230779 7 0.3044955209900731 1.261384684340445 8 0.2129281300256218 1.0080721852187817 9 0.15161116860924043 0.807499588139138 >10 0.9337908395543743 10.687631177528974 >50 0.11999941054128804 4.922579945723153 >100 0.06335483385474146 6.652372324746357 >500 0.0014684895245695637 0.5050360293503172 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.49589051626497E-5 0.0 22-23 0.0 0.0 0.0 9.983562065059878E-5 0.0 24-25 0.0 0.0 0.0 4.243013877650449E-4 0.0 26-27 0.0 0.0 0.0 8.2364387036744E-4 0.0 28-29 0.0 0.0 0.0 0.0030949042401685624 0.0 30-31 0.0 0.0 0.0 0.013502767692993486 0.0 32-33 0.0 0.0 0.0 0.03551652204645052 0.0 34-35 0.0 0.0 0.0 0.06783830423208187 0.0 36-37 0.0 0.0 0.0 0.12347170383962805 0.0 38-39 0.0 0.0 0.0 0.2088810773062153 0.0 40-41 0.0 0.0 0.0 0.3036749991139589 0.0 42-43 0.0 0.0 0.0 0.3954988112073471 0.0 44-45 0.0 0.0 0.0 0.5077639666289455 0.0 46-47 0.0 0.0 0.0 0.6487318629875909 0.0 48-49 0.0 0.0 0.0 0.800631759807477 0.0 50-51 0.0 0.0 0.0 0.9649611913983627 0.0 52-53 0.0 0.0 0.0 1.1228512254572847 0.0 54-55 0.0 0.0 0.0 1.2913487942103328 0.0 56-57 0.0 0.0 0.0 1.489472583391446 2.49589051626497E-5 58-59 0.0 0.0 0.0 1.709560209115691 7.48767154879491E-5 60-61 0.0 0.0 0.0 1.9545568221922605 9.98356206505988E-5 62-63 0.0 0.0 0.0 2.198629955777812 9.98356206505988E-5 64-65 0.0 0.0 0.0 2.447420322439104 9.98356206505988E-5 66-67 0.0 0.0 0.0 2.728058252087937 9.98356206505988E-5 68-69 0.0 0.0 0.0 3.0447118818864736 9.98356206505988E-5 70-71 0.0 0.0 0.0 3.3926889376641363 9.98356206505988E-5 72-73 0.0 0.0 0.0 3.7409155824934244 9.98356206505988E-5 74-75 0.0 0.0 0.0 4.090864391778936 9.98356206505988E-5 76-77 0.0 0.0 0.0 4.459482462126109 9.98356206505988E-5 78-79 0.0 0.0 0.0 4.870805219206577 9.98356206505988E-5 80-81 0.0 0.0 0.0 5.325256964408101 9.98356206505988E-5 82-83 0.0 0.0 0.0 5.802820655790241 9.98356206505988E-5 84-85 0.0 0.0 0.0 6.269102922038863 9.98356206505988E-5 86-87 0.0 0.0 0.0 6.744794695533804 9.98356206505988E-5 88-89 0.0 0.0 0.0 7.263016443425899 9.98356206505988E-5 90-91 0.0 2.49589051626497E-5 0.0 7.829309042661258 9.98356206505988E-5 92-93 0.0 7.48767154879491E-5 0.0 8.425327697945335 9.98356206505988E-5 94-95 0.0 1.2479452581324848E-4 0.0 9.030081970036335 9.98356206505988E-5 96-97 0.0 1.4975343097589817E-4 0.0 9.617639556470271 9.98356206505988E-5 98-99 0.0 1.4975343097589817E-4 0.0 10.233400705738003 9.98356206505988E-5 100-101 0.0 1.4975343097589817E-4 0.0 10.912282926162074 9.98356206505988E-5 102-103 0.0 1.4975343097589817E-4 0.0 11.634743395000132 9.98356206505988E-5 104-105 0.0 1.996712413011976E-4 0.0 12.34267778103353 9.98356206505988E-5 106-107 0.0 2.4958905162649695E-4 0.0 13.03334060469437 9.98356206505988E-5 108-109 0.0 2.4958905162649695E-4 0.0 13.739777456418008 9.98356206505988E-5 110-111 0.0 2.4958905162649695E-4 0.0 14.501398447456264 9.98356206505988E-5 112-113 0.0 2.4958905162649695E-4 0.0 15.310566152829367 9.98356206505988E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1900 0.0 56.415356 1 GTATCAA 3370 0.0 47.533615 1 ATCAACG 3795 0.0 39.524017 3 GTACATG 10715 0.0 39.236565 1 TCAACGC 3785 0.0 39.156673 4 CAACGCA 3885 0.0 38.145924 5 TACATGG 10960 0.0 37.74388 2 ACATGGG 11000 0.0 36.74087 3 AACGCAG 4085 0.0 36.416737 6 CATGGGA 7620 0.0 34.291138 4 TATCAAC 4520 0.0 33.842857 2 ACGCAGA 5075 0.0 29.188242 7 CATGGGG 3740 0.0 28.487463 4 CGCAGAG 5230 0.0 28.323198 8 ATGGGAT 3640 0.0 27.142292 5 GAGTACA 7570 0.0 25.408855 1 ATGGGAG 3295 0.0 24.204113 5 AGTACAT 7650 0.0 23.73071 2 CCTATAC 640 0.0 23.250504 3 GCAGAGT 6670 0.0 21.49492 9 >>END_MODULE