FastQCFastQC Report
Thu 26 May 2016
SRR1364336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003293
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33570.16757408926203005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.15349726675029565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33150.069.912621
GTATCAA58100.051.5800061
TCAACGC71450.040.9235654
ATCAACG71400.040.8688133
CAACGCA73700.039.67425
TATCAAC77500.038.113092
AACGCAG77250.037.8977786
GTACATG105450.037.7980231
TACATGG109800.035.9045752
ACATGGG110700.034.8057373
ACGCAGA92150.031.766017
CATGGGA75200.031.6764374
CGCAGAG93550.031.2270248
CATGGGG39400.028.2643854
TGGTATC17900.026.6152842
GCAGAGT110200.026.4549799
ATGGGAT33400.026.209885
GAGTACA77800.024.5818711
CAGAGTA104800.024.41181410-11
AGTACAT76500.024.209912
Produced by FastQC (version 0.11.5)