FastQCFastQC Report
Thu 26 May 2016
SRR1364335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612075
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.059.375961
GTATCAA29350.050.5173951
GTACATG105050.042.115551
TACATGG105800.040.6774832
ATCAACG35300.040.639073
TCAACGC35300.040.639074
CAACGCA36250.039.409845
ACATGGG107700.038.135973
AACGCAG38100.037.1786
TATCAAC39600.036.8275072
CATGGGA83750.035.3242534
ATGGGAG34150.031.3749625
ACGCAGA49300.028.7202977
CGCAGAG50600.027.8648518
GAGTACA73000.027.0810151
CATGGGG32150.026.66144
ATGGGAT33750.026.2793085
AGTACAT75900.024.6257422
GGACCGA2701.708031E-924.2472766
GTATAGA5000.023.8182411
Produced by FastQC (version 0.11.5)