##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364335_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1612075 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12555991501636 31.0 31.0 34.0 28.0 34.0 2 31.15953352046276 33.0 31.0 34.0 27.0 34.0 3 31.315886047485385 34.0 31.0 34.0 28.0 34.0 4 34.68065195477878 37.0 35.0 37.0 32.0 37.0 5 34.78576927252144 37.0 35.0 37.0 32.0 37.0 6 34.84940651024301 37.0 35.0 37.0 32.0 37.0 7 34.76908890715382 37.0 35.0 37.0 32.0 37.0 8 34.71333715863096 37.0 35.0 37.0 32.0 37.0 9 36.23215359086891 39.0 37.0 39.0 32.0 39.0 10-11 36.15800226416265 39.0 37.0 39.0 31.5 39.0 12-13 36.1696428516043 39.0 37.0 39.0 32.0 39.0 14-15 37.48374610362421 40.0 38.0 41.0 32.0 41.0 16-17 37.50555402199029 40.0 38.0 41.0 32.0 41.0 18-19 37.51964765907293 40.0 38.0 41.0 31.0 41.0 20-21 37.55210365522696 40.0 38.0 41.0 31.0 41.0 22-23 37.529176372067056 40.0 38.0 41.0 31.0 41.0 24-25 37.5638059643627 40.0 38.0 41.0 31.0 41.0 26-27 37.540804863297296 40.0 38.0 41.0 31.0 41.0 28-29 37.54836933765488 40.0 38.0 41.0 31.0 41.0 30-31 37.46194097669153 40.0 38.0 41.0 30.5 41.0 32-33 37.42633314206846 40.0 38.0 41.0 30.0 41.0 34-35 37.41047283780221 40.0 38.0 41.0 30.0 41.0 36-37 37.354438224028044 40.0 38.0 41.0 30.0 41.0 38-39 37.267492207248424 40.0 37.0 41.0 30.0 41.0 40-41 37.20697703270629 40.0 37.0 41.0 30.0 41.0 42-43 37.08395391033295 40.0 37.0 41.0 30.0 41.0 44-45 36.97081773490687 40.0 37.0 41.0 30.0 41.0 46-47 36.86447994044942 40.0 37.0 41.0 30.0 41.0 48-49 36.73219546236993 40.0 36.0 41.0 30.0 41.0 50-51 36.62008529379837 40.0 36.0 41.0 30.0 41.0 52-53 36.475041173642666 40.0 36.0 41.0 30.0 41.0 54-55 36.29883256051983 39.5 35.0 41.0 29.0 41.0 56-57 36.118308391358966 39.0 35.0 41.0 29.0 41.0 58-59 35.90892390242389 39.0 35.0 41.0 29.0 41.0 60-61 35.665758727106365 39.0 35.0 41.0 29.0 41.0 62-63 35.40244653629639 38.0 35.0 40.0 28.0 41.0 64-65 35.06410123598468 37.5 34.0 40.0 28.0 41.0 66-67 34.74783431291968 37.0 34.0 40.0 28.0 41.0 68-69 34.416425414450316 36.5 34.0 39.5 27.5 41.0 70-71 34.052601771009414 36.0 34.0 39.0 27.0 41.0 72-73 33.678916613681125 35.5 33.5 39.0 26.0 40.5 74-75 33.31519315168339 35.0 33.0 37.5 26.0 40.0 76-77 33.07237039219639 35.0 33.0 37.0 26.0 39.0 78-79 32.8987655661182 35.0 33.0 37.0 26.0 39.0 80-81 32.618787277266875 35.0 33.0 36.0 26.0 39.0 82-83 32.321721694089916 35.0 33.0 36.0 26.0 37.0 84-85 32.06332614797698 35.0 33.0 36.0 26.0 37.0 86-87 31.840264565854568 35.0 33.0 35.0 25.0 37.0 88-89 31.57125102740257 35.0 33.0 35.0 25.0 36.0 90-91 31.43752585952887 35.0 33.0 35.0 24.5 36.0 92-93 31.284644635020083 35.0 32.0 35.0 24.0 36.0 94-95 31.17577345967154 35.0 32.0 35.0 24.0 35.5 96-97 31.061192562380782 35.0 32.0 35.0 23.0 35.0 98-99 30.922880759269887 35.0 32.0 35.0 22.5 35.0 100-101 30.824398678721522 35.0 32.0 35.0 20.0 35.0 102-103 29.82243568072205 33.5 30.0 34.5 19.0 35.0 104-105 30.501856923530234 34.5 31.5 35.0 19.0 35.0 106-107 30.811955399097435 35.0 32.0 35.0 20.0 35.0 108-109 30.83261758913202 35.0 33.0 35.0 20.0 35.0 110-111 30.756254206535054 35.0 32.5 35.0 19.0 35.0 112-113 30.65109284617651 35.0 32.0 35.0 18.5 35.0 114-115 30.534201572507484 35.0 32.0 35.0 16.0 35.0 116-117 30.39242963261635 35.0 32.0 35.0 8.5 35.0 118-119 30.199652311461936 35.0 32.0 35.0 2.0 35.0 120-121 29.993739435199977 35.0 32.0 35.0 2.0 35.0 122-123 29.78543057860211 35.0 32.0 35.0 2.0 35.0 124-125 28.95326644231813 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35264961279418117 1101 2 0.18807898813973623 1101 3 0.31903970275700644 1101 4 0.4168120312241044 1101 5 0.2994980670582592 1101 6 0.29792552632198266 1101 7 0.28162801786973546 1101 8 0.132496268009767 1101 9 0.3925803338888798 1101 10-11 0.4494256612004577 1101 12-13 0.5412247460469928 1101 14-15 0.4846480702017857 1101 16-17 0.4339056831150998 1101 18-19 0.3023015946804435 1101 20-21 0.4279469544672878 1101 22-23 0.5120767724074256 1101 24-25 0.46321960668624484 1101 26-27 0.6883938533797576 1101 28-29 0.46159343826619903 1101 30-31 0.5207024582601747 1101 32-33 0.4738585527392942 1101 34-35 0.541248283805821 1101 36-37 0.42325848111837416 1101 38-39 0.5785376785881837 1101 40-41 0.3987072683022461 1101 42-43 0.5472202908200998 1101 44-45 0.5913648006251151 1101 46-47 0.5957860414651819 1101 48-49 0.5861440585424376 1101 50-51 0.5187289379024307 1101 52-53 0.4699852559816833 1101 54-55 0.6196462670247911 1101 56-57 0.3988920391325408 1101 58-59 0.41660571803203084 1101 60-61 0.3763687525822661 1101 62-63 0.4927679478359721 1101 64-65 0.6347284219495606 1101 66-67 0.5206799162771176 1101 68-69 0.4836053130592788 1101 70-71 0.4514421082468303 1101 72-73 0.5551547177669036 1101 74-75 0.5517634840402295 1101 76-77 0.49913160183904637 1101 78-79 0.46534004648519556 1101 80-81 0.484076714948074 1101 82-83 0.4852528238444549 1101 84-85 0.4672180329751825 1101 86-87 0.4477419405886991 1101 88-89 0.5742353623011631 1101 90-91 0.47629171062570563 1101 92-93 0.44773835241470294 1101 94-95 0.43012802747653467 1101 96-97 0.4002929855652475 1101 98-99 0.3881587232044197 1101 100-101 0.38269891493677477 1101 102-103 0.1910609347583332 1101 104-105 0.3037658870671791 1101 106-107 0.38046851316551766 1101 108-109 0.39358117068469056 1101 110-111 0.34721798308984475 1101 112-113 0.4086651576695921 1101 114-115 0.4094383318537176 1101 116-117 0.33288038911802076 1101 118-119 0.38334624667033523 1101 120-121 0.336884783769424 1101 122-123 0.2885247754631912 1101 124-125 0.25828847856417525 1102 1 0.19850776576175022 1102 2 0.17177147031418372 1102 3 0.30264204869255806 1102 4 0.33972508777677746 1102 5 0.3201933089071076 1102 6 0.3191312828429602 1102 7 0.1923045492110873 1102 8 0.23008254691947627 1102 9 0.37841794274238083 1102 10-11 0.40041924434950005 1102 12-13 0.3083578693583817 1102 14-15 0.28881078174690344 1102 16-17 0.48347820186904045 1102 18-19 0.38441831396841053 1102 20-21 0.44485666729361384 1102 22-23 0.4217659548620887 1102 24-25 0.4447618473843633 1102 26-27 0.352956858610753 1102 28-29 0.3153738331903142 1102 30-31 0.34269284398536826 1102 32-33 0.37576851455008864 1102 34-35 0.25480487064176316 1102 36-37 0.1874913213595235 1102 38-39 0.22947090712929707 1102 40-41 0.17461304099757058 1102 42-43 0.06542887263929487 1102 44-45 0.05940474854297406 1102 46-47 0.09162375454474159 1102 48-49 0.2102829872452432 1102 50-51 0.15191169675074434 1102 52-53 0.24722126199282002 1102 54-55 0.2596192570349132 1102 56-57 0.19626828323460188 1102 58-59 0.21313396734693413 1102 60-61 0.27086023171602136 1102 62-63 0.26174982910087863 1102 64-65 0.3136094818745221 1102 66-67 0.2687081136720053 1102 68-69 0.13332556259961592 1102 70-71 0.04517549721887093 1102 72-73 0.06885320645046988 1102 74-75 0.04148418908704343 1102 76-77 0.05466575459507084 1102 78-79 0.252316267117088 1102 80-81 0.22463180749115708 1102 82-83 0.20221547964793274 1102 84-85 0.24762965255933267 1102 86-87 0.18314768684148675 1102 88-89 0.25760866182408293 1102 90-91 0.1663860467887126 1102 92-93 0.07141955501356279 1102 94-95 0.07771357294360826 1102 96-97 0.05463537125339002 1102 98-99 0.06884955281681826 1102 100-101 0.03526511488944806 1102 102-103 -0.16730917042892202 1102 104-105 -0.09240121895735598 1102 106-107 -0.05597820049037594 1102 108-109 0.04826981849828016 1102 110-111 0.021314680938118613 1102 112-113 -0.021991412451576764 1102 114-115 -0.17180925791869583 1102 116-117 -0.2467266052997097 1102 118-119 -0.2627898810536813 1102 120-121 -0.5034344297639031 1102 122-123 -0.5306995664940146 1102 124-125 -0.5440556812830835 1103 1 0.35898712886942263 1103 2 0.4062107366545291 1103 3 0.5019448867086247 1103 4 0.540081160853191 1103 5 0.4719851114809117 1103 6 0.4312222765611935 1103 7 0.4330881995283633 1103 8 0.4526379919637691 1103 9 0.6195932082951572 1103 10-11 0.635287043442915 1103 12-13 0.5066896318875393 1103 14-15 0.644056471445758 1103 16-17 0.5153094265606342 1103 18-19 0.5765461238620091 1103 20-21 0.6805724834820168 1103 22-23 0.6417339162478086 1103 24-25 0.6942303437121566 1103 26-27 0.7758618618754198 1103 28-29 0.7234640538122932 1103 30-31 0.8815526935066771 1103 32-33 0.8212143768202509 1103 34-35 0.8462444945753163 1103 36-37 0.7235867002997409 1103 38-39 0.6890494828080378 1103 40-41 0.7835645979757757 1103 42-43 0.6504227703907972 1103 44-45 0.6295536419584806 1103 46-47 0.6499033041220699 1103 48-49 0.665230405118244 1103 50-51 0.7201725486912238 1103 52-53 0.6894274311271715 1103 54-55 0.6660266541472453 1103 56-57 0.6833625343808478 1103 58-59 0.554669866375356 1103 60-61 0.545250043060264 1103 62-63 0.5358091924691379 1103 64-65 0.5284033807842263 1103 66-67 0.5940392131335912 1103 68-69 0.5306541458790264 1103 70-71 0.5765870566003386 1103 72-73 0.5401521891195031 1103 74-75 0.47527461195911513 1103 76-77 0.4778300853239088 1103 78-79 0.5283823871149451 1103 80-81 0.6586748620031315 1103 82-83 0.5673141652395302 1103 84-85 0.51362507189166 1103 86-87 0.5410367793799438 1103 88-89 0.6160502503486747 1103 90-91 0.5785634347368109 1103 92-93 0.6624259410202313 1103 94-95 0.5321683953124392 1103 96-97 0.5610137772563242 1103 98-99 0.4945639067819769 1103 100-101 0.4571695448467814 1103 102-103 0.37804309507607314 1103 104-105 0.5451373453710531 1103 106-107 0.6008888559094565 1103 108-109 0.5303789379937704 1103 110-111 0.6197785864640188 1103 112-113 0.5763906954373361 1103 114-115 0.5568250907187604 1103 116-117 0.467442772270946 1103 118-119 0.5239649460927112 1103 120-121 0.35127450818426453 1103 122-123 0.39959074996873767 1103 124-125 0.337434020177259 1104 1 0.34110959018691034 1104 2 0.33300660538047566 1104 3 0.39070320528306013 1104 4 0.43426730486892495 1104 5 0.3584754963888983 1104 6 0.37784849066224524 1104 7 0.4277440585570531 1104 8 0.4155585021989694 1104 9 0.49400581093965457 1104 10-11 0.5279459036164766 1104 12-13 0.44305900398013875 1104 14-15 0.45193451054448985 1104 16-17 0.4654016992550183 1104 18-19 0.48331790498536265 1104 20-21 0.44015376358729696 1104 22-23 0.463035958689872 1104 24-25 0.4894110818801849 1104 26-27 0.5294548841797777 1104 28-29 0.562027751049655 1104 30-31 0.5141429594643725 1104 32-33 0.5016162009050049 1104 34-35 0.5205078514620212 1104 36-37 0.47522446454824063 1104 38-39 0.38548026408624736 1104 40-41 0.48123526188609844 1104 42-43 0.42735243283301116 1104 44-45 0.42392610648809637 1104 46-47 0.40409014804427557 1104 48-49 0.45203441237022446 1104 50-51 0.4088164477721534 1104 52-53 0.3003593109542422 1104 54-55 0.30442411514351875 1104 56-57 0.1296664364778053 1104 58-59 0.23766396484509755 1104 60-61 0.10365757662366093 1104 62-63 0.14199109106182561 1104 64-65 0.23131582011721719 1104 66-67 0.17522743455289458 1104 68-69 0.2236307872611505 1104 70-71 0.09579940484403693 1104 72-73 0.08401595350731839 1104 74-75 0.05609056428161807 1104 76-77 0.028788094992755475 1104 78-79 0.13943768040280702 1104 80-81 0.08176147999417793 1104 82-83 0.09485463978288777 1104 84-85 0.09807876276563121 1104 86-87 0.09081542973034118 1104 88-89 0.11050797160195458 1104 90-91 0.13234626018148887 1104 92-93 0.08580427123948908 1104 94-95 0.0957051106479625 1104 96-97 0.03649963745490581 1104 98-99 0.031152785775244496 1104 100-101 -0.028992171418572354 1104 102-103 -0.1662093371834601 1104 104-105 -0.04255036988399752 1104 106-107 0.02745184512548704 1104 108-109 0.08243321421947059 1104 110-111 0.03517181218081333 1104 112-113 0.08851522266234468 1104 114-115 -0.041783025220915704 1104 116-117 -0.08959098350835504 1104 118-119 -0.07566783969059898 1104 120-121 -0.14542274473353345 1104 122-123 -0.21886351841260065 1104 124-125 -0.33359742557255245 1105 1 0.19713193453037903 1105 2 0.14938307481346058 1105 3 0.09668259719636652 1105 4 0.2938386683685579 1105 5 0.2554829840172701 1105 6 0.2754646602134514 1105 7 0.18820855348473486 1105 8 0.21004713960395094 1105 9 0.2637394218005582 1105 10-11 0.25264688862188933 1105 12-13 0.23486717097824084 1105 14-15 0.4126523314901078 1105 16-17 0.40549058363016854 1105 18-19 0.3442503956024936 1105 20-21 0.32616386535319464 1105 22-23 0.35613543934294967 1105 24-25 0.33375841932554096 1105 26-27 0.39301084432759126 1105 28-29 0.38461689562792145 1105 30-31 0.3514834768137405 1105 32-33 0.49142725224833583 1105 34-35 0.3483899225342171 1105 36-37 0.3329207682516042 1105 38-39 0.3625528806803615 1105 40-41 0.2863054646023997 1105 42-43 0.18575875895503202 1105 44-45 0.19270423805990333 1105 46-47 0.28270644588949523 1105 48-49 0.15357269619322977 1105 50-51 0.1783455112410337 1105 52-53 0.20667500803712358 1105 54-55 0.11517254673665889 1105 56-57 0.05572579103619546 1105 58-59 -0.043219622719433914 1105 60-61 0.018694071476680563 1105 62-63 0.08382833859544547 1105 64-65 0.010217996693150155 1105 66-67 -0.028389447593340833 1105 68-69 0.03089300154845631 1105 70-71 0.06502893627497741 1105 72-73 0.03415027548356875 1105 74-75 0.029864948760433663 1105 76-77 0.029982507158187843 1105 78-79 0.0996990739783854 1105 80-81 0.046122564907499 1105 82-83 0.07970989370834047 1105 84-85 0.10667849672790908 1105 86-87 -0.08495509516702171 1105 88-89 -0.009594808208031935 1105 90-91 -0.02677488046825971 1105 92-93 -0.02661885954793064 1105 94-95 -0.1159910992309463 1105 96-97 -0.04900302199747841 1105 98-99 -0.11582456200929414 1105 100-101 -0.2882871395190989 1105 102-103 -0.3258652603899961 1105 104-105 -0.19425088761673237 1105 106-107 -0.19668493209205096 1105 108-109 -0.13365290384767547 1105 110-111 -0.17970534924953796 1105 112-113 -0.22372338670459513 1105 114-115 -0.29264203178812664 1105 116-117 -0.3882753225764688 1105 118-119 -0.3018696796250744 1105 120-121 -0.3246383756853106 1105 122-123 -0.41993831281009975 1105 124-125 -0.5429312938285626 1106 1 0.039710978726674284 1106 2 0.07529409895871098 1106 3 0.11517192335938375 1106 4 0.21754236625125145 1106 5 0.03126473812566388 1106 6 -0.004871188668516879 1106 7 0.058950740745977726 1106 8 0.06922828093482991 1106 9 0.24711182865060266 1106 10-11 0.2120055415940456 1106 12-13 0.0897548531721597 1106 14-15 0.2585155593380506 1106 16-17 0.17868711163168882 1106 18-19 0.12053019516190489 1106 20-21 -0.021191577915118387 1106 22-23 0.09755284316752721 1106 24-25 0.07721629021616394 1106 26-27 0.2649983198466188 1106 28-29 0.12511591900828734 1106 30-31 0.2562693059188348 1106 32-33 0.22116459963242363 1106 34-35 0.2275393335500624 1106 36-37 0.2532905128636074 1106 38-39 0.1404042651052677 1106 40-41 0.2557225575851447 1106 42-43 0.09060902661953918 1106 44-45 0.0945149790422164 1106 46-47 0.14598196788111295 1106 48-49 0.07771131069434745 1106 50-51 7.331083026969054E-4 1106 52-53 0.04963596857331254 1106 54-55 0.06729426052293519 1106 56-57 0.03285027707757138 1106 58-59 -0.061259887214390574 1106 60-61 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-1.266489934193796 2216 106-107 -1.1401541210868764 2216 108-109 -1.0194344819621222 2216 110-111 -1.0073706267023184 2216 112-113 -0.9400295293307543 2216 114-115 -1.005780507772613 2216 116-117 -1.1016772312172094 2216 118-119 -1.02910635764216 2216 120-121 -0.9280141985453341 2216 122-123 -0.9553246440657865 2216 124-125 -0.775466890740308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22213.0 3 4474.0 4 2931.0 5 2440.0 6 2747.0 7 3422.0 8 3678.0 9 3879.0 10 4207.0 11 4996.0 12 5570.0 13 6081.0 14 6063.0 15 5378.0 16 4913.0 17 4474.0 18 4291.0 19 4061.0 20 4054.0 21 4202.0 22 5146.0 23 6901.0 24 10314.0 25 15604.0 26 18209.0 27 17580.0 28 20152.0 29 25266.0 30 29720.0 31 36176.0 32 46764.0 33 62599.0 34 87096.0 35 133107.0 36 255051.0 37 503591.0 38 234056.0 39 669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.072940831846964 18.736478359790134 12.394417944201784 23.796162864161122 2 15.333615399313613 20.625764618007388 39.83148005231042 24.209139930368583 3 18.42623569120483 26.27694346207553 29.30411474206793 25.992706104651703 4 12.14420463688603 16.49721740932154 37.77443614619721 33.58414180759522 5 12.946264313435938 39.67233051123514 34.229331414017004 13.15207376131192 6 31.42026726500139 37.03505371113141 18.137497021563878 13.407182002303324 7 28.544213534620035 33.16218256619453 21.291180245516674 17.00242365366876 8 26.57306869668352 35.57396940087043 20.170066845649572 17.682895056796472 9 27.244203898701986 16.285284493587458 19.520927996526215 36.949583611184345 10-11 24.49727013067332 27.64176075098313 27.787504726056223 20.073464392287324 12-13 24.97998681812895 25.196975923700226 27.784468654286048 22.038568603884777 14-15 22.292556245248733 26.305224433561953 25.25688253515124 26.14533678603807 16-17 22.3991509077279 29.676885280425935 25.858730474305002 22.065233337541162 18-19 22.364226963456094 28.593808784891767 27.236179221245667 21.805785030406476 20-21 23.479125809632247 27.69546320594985 26.944722467851207 21.880688516566696 22-23 23.088783090116774 27.837755687545556 26.74646030736783 22.327000914969837 24-25 22.61721694089915 27.912752198253806 27.019059286943847 22.4509715739032 26-27 22.603049672952462 28.17909470064814 26.787099479583603 22.430756146815796 28-29 22.50107005278927 28.09567822737226 26.929196622975425 22.474055096863037 30-31 22.48213633270495 27.804079348862427 27.06909311066324 22.644691207769384 32-33 22.357389966074695 28.023021318539943 26.89112328179094 22.728465433594426 34-35 22.866907641832412 27.904403121221836 26.642416136858344 22.586273100087404 36-37 22.925629631651407 27.53134394926782 26.66351751331437 22.879508905766393 38-39 22.51157360739867 27.78068983228427 26.884700630802698 22.823035929514358 40-41 22.72973850644499 27.587075347077807 27.032848830988055 22.650337315489153 42-43 22.58261318895501 27.64036224435676 26.87606114160692 22.900963425081308 44-45 22.466151878286954 27.67900454947539 27.083925228311212 22.770918343926443 46-47 22.689746252343255 27.853901247709473 26.661089999590587 22.795262500356685 48-49 22.561512336584837 27.724237395902797 26.84198936153715 22.87226090597522 50-51 22.588061969821503 27.5784005086612 26.949552595257664 22.883984926259636 52-53 22.74626595321012 27.654153882800742 26.820250884273396 22.779329279715743 54-55 22.387080125159116 27.61022141054622 26.984580912986623 23.01811755130804 56-57 22.4306630990069 27.805972986980443 26.96305288620346 22.800311027809197 58-59 22.550410467065245 27.35570379872784 26.981649634692378 23.112236099514536 60-61 22.35483717887759 27.547074070731213 27.216100140162236 22.88198861022896 62-63 22.365609607295976 27.54337367596188 27.163860796065176 22.92715592067697 64-65 22.24007731762912 27.514133388418422 27.059247176656577 23.18654211729588 66-67 22.205294040652863 27.78704485437387 26.851862765106798 23.155798339866468 68-69 22.292734036673174 27.516015590348275 27.110159098226266 23.081091274752282 70-71 22.281219022285963 27.464786984986784 27.11771252185111 23.136281470876142 72-73 22.40233295472239 27.46727269230841 26.916477516279137 23.213916836690068 74-75 22.274640146298736 27.807553639975087 26.849174092022306 23.068632121703875 76-77 22.440952697023363 27.480389937067905 26.984749279646163 23.093908086262566 78-79 22.493542327838835 27.489398499785366 27.016797391621527 23.000261780754276 80-81 22.492697991664684 27.819341319612555 26.898033660730874 22.789927027991887 82-83 22.540333286188922 27.68136952039244 26.801195731197442 22.977101462221196 84-85 22.58283929886289 27.50282711574643 26.875806030394518 23.038527554996165 86-87 22.590175252932383 27.414616807321647 26.913513082634662 23.08169485711131 88-89 22.688372390215427 27.37563248823734 26.89408379040395 23.041911331143282 90-91 22.6626366451777 27.557220773944017 26.90098274420891 22.879159836669373 92-93 22.708299215411873 27.222658518066368 26.995122998208497 23.073919268313258 94-95 22.452973097708764 27.132773303160683 27.086124799634753 23.328128799495797 96-97 22.74705107499951 27.081942135905347 26.991189281113947 23.179817507981195 98-99 22.71171668548317 27.091919655595948 26.795497686190338 23.400865972730543 100-101 22.41856587354832 27.245759991365055 27.050109037876823 23.285565097209805 102-103 22.49748845495704 27.10539432420286 26.998170968679432 23.398946252160673 104-105 22.527862615663235 27.264995783349455 26.919072256238362 23.288069344748944 106-107 22.486956953293173 27.219179016979222 26.930650074132895 23.36321395559471 108-109 22.594227392606097 27.332589143786045 26.577802287716 23.495381175891858 110-111 22.466962128691296 27.61025314637901 26.749796765177784 23.172987959751907 112-113 22.367359142850585 27.68481074612866 26.83306441957742 23.11476569144333 114-115 22.406090224351217 27.770892032758777 26.652764905776955 23.170252837113047 116-117 22.363357609943392 27.77049407104204 26.680512429704994 23.185635889309573 118-119 22.31267603973017 27.994075662834096 26.360693828877512 23.33255446855822 120-121 22.439828783727318 28.027746490975286 26.47276052583335 23.05966419946404 122-123 22.481416747182465 28.201972391482023 26.41369973061456 22.902911130720952 124-125 22.57874613492354 28.216104958246785 26.292352227608458 22.912796679221216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 976.0 1 790.5 2 539.0 3 413.0 4 313.0 5 239.0 6 209.0 7 203.5 8 198.5 9 212.0 10 205.5 11 188.5 12 198.5 13 211.0 14 247.0 15 284.5 16 300.5 17 374.5 18 525.0 19 717.0 20 945.5 21 1311.0 22 1820.5 23 2525.0 24 3566.0 25 4919.5 26 6738.5 27 8984.0 28 11305.0 29 14456.5 30 18500.5 31 21971.0 32 25871.0 33 29544.0 34 33044.5 35 36261.0 36 39889.5 37 44533.5 38 47786.0 39 49713.5 40 50842.5 41 53128.0 42 55454.5 43 57706.0 44 60228.0 45 62086.5 46 61777.0 47 60924.5 48 61218.5 49 60381.0 50 59560.5 51 58250.5 52 57445.5 53 56966.5 54 55450.0 55 51534.0 56 45599.5 57 40896.5 58 37501.0 59 33187.5 60 27429.0 61 22197.5 62 17874.0 63 13763.5 64 10429.5 65 8093.5 66 5929.0 67 4699.0 68 3491.5 69 2102.5 70 1447.5 71 1036.0 72 790.5 73 602.0 74 464.5 75 376.5 76 255.0 77 141.5 78 75.5 79 49.0 80 28.0 81 18.5 82 19.5 83 13.0 84 10.0 85 10.0 86 7.0 87 6.0 88 9.0 89 7.0 90 1.5 91 0.5 92 1.0 93 2.0 94 2.0 95 0.5 96 1.0 97 3.0 98 3.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047702495231301274 2 0.009863064683715088 3 0.0026053378409813437 4 0.005458803095389482 5 0.02419242280911248 6 0.030457639998139045 7 0.0028534652544081386 8 1.8609556007009599E-4 9 0.0 10-11 9.304778003504799E-5 12-13 7.753981669587332E-4 14-15 3.411751934618426E-4 16-17 3.101592667834933E-5 18-19 3.101592667834933E-5 20-21 3.101592667834933E-5 22-23 0.0 24-25 0.0 26-27 3.101592667834933E-5 28-29 3.101592667834933E-4 30-31 2.4812741342679465E-4 32-33 2.4812741342679465E-4 34-35 8.684459469937814E-4 36-37 9.304778003504799E-5 38-39 1.2406370671339732E-4 40-41 5.272707535319387E-4 42-43 8.684459469937814E-4 44-45 0.0013026689204906718 46-47 6.203185335669866E-5 48-49 0.0 50-51 0.0 52-53 0.0010545415070638774 54-55 0.0029154971077648374 56-57 3.101592667834933E-5 58-59 5.58286680210288E-4 60-61 8.994618736721307E-4 62-63 5.272707535319387E-4 64-65 0.0014577485538824187 66-67 0.002233146720841152 68-69 0.00322565637454833 70-71 0.0017989237473442614 72-73 0.0026053378409813437 74-75 0.0019229874540576586 76-77 0.0012406370671339732 78-79 0.002419242280911248 80-81 0.0011475892870989252 82-83 4.342229734968907E-4 84-85 4.962548268535893E-4 86-87 0.002078067087449405 88-89 0.005489819022067832 90-91 4.6523890017524E-4 92-93 0.0011786052137772746 94-95 0.0011786052137772746 96-97 2.79143340105144E-4 98-99 9.3047780035048E-4 100-101 0.0012716529938123227 102-103 0.001209621140455624 104-105 8.374300203154321E-4 106-107 0.006513344602453359 108-109 0.0018919715273793092 110-111 0.0014577485538824187 112-113 0.002109083014127755 114-115 0.0030395608144782346 116-117 0.0018299396740226106 118-119 0.04686506521098584 120-121 0.01212722733123459 122-123 0.004497309368360653 124-125 0.0026983856210163917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1612075.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.28459515727916 #Duplication Level Percentage of deduplicated Percentage of total 1 83.65117872355763 57.95738052290893 2 9.971354451438934 13.817225126753598 3 2.954428673255682 6.140891836427318 4 1.223394629832391 3.390496065821064 5 0.6312359345271654 2.1867463086220713 6 0.35970744767595475 1.4953310932372017 7 0.23979521826821504 1.1629880232855268 8 0.16577814307317804 0.9188697223000513 9 0.12460019278861174 0.7769586522490108 >10 0.6126873750199942 7.864502578121639 >50 0.047867374695686624 2.253517232523676 >100 0.017971835866580556 2.0350928377498723 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 3.101592667834933E-5 0.0 0.0 0.0 0.0 16-17 6.203185335669866E-5 0.0 6.203185335669866E-5 0.0 0.0 18-19 9.304778003504799E-5 0.0 6.203185335669866E-5 0.0 0.0 20-21 1.2406370671339732E-4 0.0 6.203185335669866E-5 6.203185335669866E-5 0.0 22-23 1.2406370671339732E-4 0.0 6.203185335669866E-5 6.203185335669866E-5 0.0 24-25 2.4812741342679465E-4 0.0 6.203185335669866E-5 3.4117519346184267E-4 0.0 26-27 2.4812741342679465E-4 0.0 6.203185335669866E-5 0.0011786052137772746 0.0 28-29 2.7914334010514395E-4 0.0 6.203185335669866E-5 0.0045283252950390025 0.0 30-31 3.101592667834933E-4 0.0 6.203185335669866E-5 0.015787106679279808 0.0 32-33 3.101592667834933E-4 0.0 6.203185335669866E-5 0.04156134174898811 0.0 34-35 3.101592667834933E-4 0.0 6.203185335669866E-5 0.08045531380363816 0.0 36-37 3.7219112014019197E-4 0.0 6.203185335669866E-5 0.14537164834142333 0.0 38-39 4.3422297349689063E-4 0.0 6.203185335669866E-5 0.24238946699130004 0.0 40-41 4.3422297349689063E-4 0.0 6.203185335669866E-5 0.34688212397065893 0.0 42-43 4.6523890017524E-4 0.0 6.203185335669866E-5 0.44923468200921174 0.0 44-45 4.962548268535893E-4 0.0 6.203185335669866E-5 0.5724299427756152 0.0 46-47 6.513344602453359E-4 0.0 6.203185335669866E-5 0.7260518276134795 0.0 48-49 8.064140936370827E-4 0.0 6.203185335669866E-5 0.8922971946094319 0.0 50-51 8.064140936370827E-4 0.0 6.203185335669866E-5 1.07609757610533 0.0 52-53 8.064140936370827E-4 0.0 6.203185335669866E-5 1.2509653707178636 0.0 54-55 8.064140936370827E-4 0.0 6.203185335669866E-5 1.4372470263480297 0.0 56-57 8.064140936370827E-4 0.0 6.203185335669866E-5 1.654420544949832 0.0 58-59 8.684459469937813E-4 0.0 6.203185335669866E-5 1.9045329776840407 0.0 60-61 8.684459469937813E-4 0.0 6.203185335669866E-5 2.18187739404184 0.0 62-63 9.3047780035048E-4 0.0 6.203185335669866E-5 2.456368345145232 0.0 64-65 9.3047780035048E-4 0.0 6.203185335669866E-5 2.731448598855512 0.0 66-67 0.0010545415070638772 0.0 6.203185335669866E-5 3.0284881286540637 0.0 68-69 0.001116573360420576 0.0 6.203185335669866E-5 3.3745638385310857 0.0 70-71 0.0012096211404556238 0.0 6.203185335669866E-5 3.756400911868244 0.0 72-73 0.0012406370671339732 0.0 6.203185335669866E-5 4.140378084146209 0.0 74-75 0.0012406370671339732 0.0 6.203185335669866E-5 4.519485755935673 0.0 76-77 0.0012406370671339732 0.0 6.203185335669866E-5 4.931346246297474 0.0 78-79 0.0012406370671339732 0.0 6.203185335669866E-5 5.387900687002777 0.0 80-81 0.001302668920490672 0.0 6.203185335669866E-5 5.891816447745917 0.0 82-83 0.001302668920490672 0.0 6.203185335669866E-5 6.413876525595893 0.0 84-85 0.0013336848471690215 0.0 6.203185335669866E-5 6.924553758354915 0.0 86-87 0.0013647007738473707 0.0 6.203185335669866E-5 7.4419614472031395 0.0 88-89 0.0013647007738473707 0.0 6.203185335669866E-5 7.998728347006188 0.0 90-91 0.0013647007738473707 0.0 6.203185335669866E-5 8.620628692833769 0.0 92-93 0.0013647007738473707 0.0 6.203185335669866E-5 9.27338988570631 0.0 94-95 0.0013647007738473707 0.0 6.203185335669866E-5 9.925995998945458 0.0 96-97 0.0013647007738473707 0.0 6.203185335669866E-5 10.550997937440876 0.0 98-99 0.0014887644805607679 0.0 6.203185335669866E-5 11.199447916505125 0.0 100-101 0.0017058759673092131 0.0 6.203185335669866E-5 11.924569266318255 0.0 102-103 0.001954003380736008 0.0 6.203185335669866E-5 12.694942853155094 0.0 104-105 0.0022021307941628024 0.0 6.203185335669866E-5 13.45095606593986 0.0 106-107 0.002233146720841152 0.0 6.203185335669866E-5 14.198905137788254 0.0 108-109 0.002233146720841152 0.0 6.203185335669866E-5 14.944248871795669 0.0 110-111 0.002233146720841152 0.0 6.203185335669866E-5 15.746103624211031 0.0 112-113 0.0022951785741978504 0.0 6.203185335669866E-5 16.59789401857854 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1755 0.0 59.37596 1 GTATCAA 2935 0.0 50.517395 1 GTACATG 10505 0.0 42.11555 1 TACATGG 10580 0.0 40.677483 2 ATCAACG 3530 0.0 40.63907 3 TCAACGC 3530 0.0 40.63907 4 CAACGCA 3625 0.0 39.40984 5 ACATGGG 10770 0.0 38.13597 3 AACGCAG 3810 0.0 37.178 6 TATCAAC 3960 0.0 36.827507 2 CATGGGA 8375 0.0 35.324253 4 ATGGGAG 3415 0.0 31.374962 5 ACGCAGA 4930 0.0 28.720297 7 CGCAGAG 5060 0.0 27.864851 8 GAGTACA 7300 0.0 27.081015 1 CATGGGG 3215 0.0 26.6614 4 ATGGGAT 3375 0.0 26.279308 5 AGTACAT 7590 0.0 24.625742 2 GGACCGA 270 1.708031E-9 24.247276 6 GTATAGA 500 0.0 23.818241 1 >>END_MODULE