FastQCFastQC Report
Thu 26 May 2016
SRR1364335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612075
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41890.2598514337112107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37680.23373602344804056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.13181768838298466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.079.355791
GTATCAA58550.054.428211
ATCAACG73550.043.3914383
TCAACGC74100.043.0693664
CAACGCA75450.042.140915
TATCAAC77250.041.3902172
AACGCAG78500.040.7096566
GTACATG96400.038.8042951
TACATGG98800.037.7861372
TGGTATC12900.036.9251442
ACATGGG102350.035.4865573
ACGCAGA93000.034.227047
CGCAGAG93600.033.944078
CATGGGA82600.031.428844
GCAGAGT104400.029.9766949
GTGGTAT16950.029.519351
ATGGGAG34450.028.8635145
ATGGGAT31600.027.5098175
GAGTACT56800.027.49663212-13
CATGGGG29000.026.4858344
Produced by FastQC (version 0.11.5)