FastQCFastQC Report
Thu 26 May 2016
SRR1364334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947389
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21500.044.5839921
GGTATCA15550.043.6481671
ATCAACG22750.039.2465063
CAACGCA23250.038.4034775
TCAACGC24100.037.048054
GTACATG61550.035.3066371
AACGCAG26200.034.530276
TACATGG64850.032.9523772
TATCAAC28100.032.834292
ACATGGG65650.031.9153123
GTACGCG800.005364713329.7481789
CATGGGG45750.028.7536034
ACGCAGA31800.028.44077
CGCAGAG31750.028.1085138
TATTCCG2757.4578566E-1125.9747185
ATTCCGT2901.4551915E-1024.6286666
CATGGGA27300.023.5479034
GAGTACA50350.023.5312581
AGTACAT48900.023.3719352
ACTATAC6050.021.6450423
Produced by FastQC (version 0.11.5)