FastQCFastQC Report
Thu 26 May 2016
SRR1364334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947389
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.11795280757979018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.11389609369263153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.069.5295261
GTATCAA41000.050.395561
ATCAACG49700.040.850753
TCAACGC51150.039.692714
CAACGCA51350.039.5381165
TATCAAC52100.039.1975062
AACGCAG54150.037.6942066
GTACATG62300.033.0700381
ACGCAGA64400.031.6890437
TACATGG65450.031.3842322
CGCAGAG64850.031.1939098
ACATGGG65400.030.4068013
GCAGAGT77300.025.9388839
CATGGGG44850.025.090044
CAGAGTA73550.024.14696310-11
TAAGGTG9950.023.9353035
GAGTACT45750.023.86414112-13
CATGGGA28350.023.1015784
AGAGTAC71200.022.3116710-11
GTACTTT49250.022.04740514-15
Produced by FastQC (version 0.11.5)