FastQCFastQC Report
Thu 26 May 2016
SRR1364333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107530
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23000.052.285911
TCAACGC24800.046.5561644
GGTATCA13600.045.9633331
ATCAACG25400.045.2221033
CAACGCA25850.044.4348645
AACGCAG28700.040.224946
TATCAAC28900.040.1572462
GTACATG70050.036.119521
TACATGG73600.034.0432782
ACATGGG70400.033.8999333
ACGCAGA34450.033.50397
CGCAGAG35200.032.2822078
TAAGGTG15950.031.3433725
TAAGGTA8650.030.961614
GTAAGGT15450.028.8908234
CATGGGA31100.028.1309264
CATGGGG41550.027.931274
GGTAAGG15550.027.5568263
AGGTAAG15650.027.0004582
GTGTAAG10150.026.3941461
Produced by FastQC (version 0.11.5)