FastQCFastQC Report
Thu 26 May 2016
SRR1364333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107530
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.10097127917514814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.072.257171
GTATCAA40900.055.627981
ATCAACG49350.044.884423
TCAACGC49700.044.8079454
TATCAAC51750.043.1480032
CAACGCA51400.043.0942845
AACGCAG54850.040.5767336
TAAGGTG14450.034.6139565
ACGCAGA64750.034.274317
CGCAGAG65900.033.67628
GTAAGGT13950.032.4398774
GTACATG70750.030.9795171
ACATGGG69350.030.6521933
TACATGG70700.030.4882
AGGTAAG15500.028.0434192
CGTACGT656.3839725E-527.46044534-35
CATGGGA33550.026.6218684
GGTAAGG16600.026.5438213
CAGAGTA69750.026.44339210-11
GCAGAGT83750.026.1434359
Produced by FastQC (version 0.11.5)