FastQCFastQC Report
Thu 26 May 2016
SRR1364332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822846
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.055.309171
GTATCAA30650.050.311651
ATCAACG35250.042.7198333
TCAACGC35550.042.0244754
CAACGCA35700.041.84795
AACGCAG37450.039.8869176
TATCAAC39400.038.5222932
GTACATG92000.037.146971
TACATGG97050.035.2033922
ACATGGG96100.034.064933
ACGCAGA48350.030.8872037
CGCAGAG48500.030.7891438
CATGGGA64550.029.875624
CGTAGAC1755.686179E-727.2094693
CATGGGG35000.026.3591754
ATGGGAG29650.026.2975185
GTATAGG8200.024.6867731
GAGTACA66550.024.6027641
ATGGGAT26750.024.0307945
GCAGAGT62150.023.4525789
Produced by FastQC (version 0.11.5)