FastQCFastQC Report
Thu 26 May 2016
SRR1364332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822846
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.19458582897293572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.17330043240076232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.10137993006540322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.074.4229661
GTATCAA58250.055.5186541
ATCAACG71950.044.6879733
TCAACGC73250.043.894884
CAACGCA74000.043.4499975
TATCAAC74550.043.0495722
AACGCAG76050.042.2544066
GTACATG91150.036.6556781
GTGGTAT12850.035.2245561
ACGCAGA91650.035.185277
CGCAGAG91550.034.9637458
TACATGG96250.034.08622
ACATGGG96100.033.0241433
GCAGAGT105450.030.1292769
CATGGGA62250.030.0343344
TGGTATC14850.029.6710552
CAGAGTA102550.026.16585410-11
GAGTACT58700.025.99820112-13
AGAGTAC97000.025.20949210-11
GTAAGGT8800.025.0349544
Produced by FastQC (version 0.11.5)