FastQCFastQC Report
Thu 26 May 2016
SRR1364331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645119
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.053.4531
GTATCAA28650.049.450171
ATCAACG30700.044.9792563
CAACGCA31350.044.045335
TCAACGC31400.043.9765324
AACGCAG33050.041.5958826
GTACATG84400.040.695731
TATCAAC36250.038.0927732
TACATGG89350.037.7037472
ACATGGG87600.036.8942223
ACGCAGA40800.033.539667
CGCAGAG42450.032.0948688
CATGGGA62650.031.1612874
CATGGGG34200.029.7599814
GCAGAGT50550.026.3635969
ATGGGAG26550.026.0041645
ATGGGAT29450.024.4539765
ATGGGGA22350.023.434445
GAGTACA63300.022.7576351
AGTACAT61050.022.618562
Produced by FastQC (version 0.11.5)