FastQCFastQC Report
Thu 26 May 2016
SRR1364331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645119
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33380.20290325502288895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29230.17767711636665798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.10060062524352342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.068.704081
GTATCAA50500.054.368531
ATCAACG61900.043.56733
TCAACGC62400.043.3136064
CAACGCA63550.042.623485
TATCAAC64550.042.1476172
AACGCAG65650.041.330626
ACGCAGA76700.035.3729827
CGCAGAG78500.034.5618828
GTACATG82350.034.0639761
GTGGTAT15400.032.8727651
TACATGG85150.032.650162
ACATGGG83800.031.8263663
GCAGAGT86950.031.1346539
TGGTATC15650.030.0514772
GTGCGTA850.0071772128.027161
CATGGGA58950.027.4687024
GAGTACT47350.027.07880612-13
CATGGGG33400.025.6666454
CAGAGTA88950.025.51824610-11
Produced by FastQC (version 0.11.5)