FastQCFastQC Report
Thu 26 May 2016
SRR1364330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482009
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92050.6211163360006586No Hit
GTACTTTTTTTTTTTTTTTTTTTTT87430.5899424362470134No Hit
TATCAACGCAGAGTACTTTTTTTTT59420.4009422344938526No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN40820.2754369237973589No Hit
GGTATCAACGCAGAGTACTTTTTTT40110.27064612967937446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39720.2680145667131576No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28380.19149681277239206No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA18800.12685483016634852No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18340.12375093538568255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1550.016.5373292
TATAACG3100.015.9248342
TCACGCA1750.015.7456654
ATCACGC1750.015.7323353
ATAACGC3150.015.6720593
TAACGCA3350.015.0325324
GTTCGCG704.384292E-714.94389616
TTCGCGC704.384292E-714.94389617
ATCAGCG400.00527269314.25254254
GTCAACG551.9711944E-413.8089473
GTATAAC4300.013.6880571
GGTATCA21550.013.6562981
TCGTTCG655.3981727E-513.16779514
TCGCGCA801.9770596E-613.07546518
TTGACCG1207.2941475E-1012.67923818
GTCTAAT1052.0127118E-812.6577731
GTTATCA3500.012.4769471
CGTCTCG701.08043E-412.22682417
GTATCAC2900.012.1121791
CTCAACG1103.848436E-812.08282853
Produced by FastQC (version 0.11.5)