##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364330_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1482009
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.555384616422707	30.0	25.0	31.0	2.0	34.0
2	25.42837526627706	30.0	25.0	31.0	2.0	34.0
3	24.85318712639397	30.0	22.0	31.0	2.0	34.0
4	28.561372434310453	35.0	27.0	37.0	2.0	37.0
5	29.123195608123837	35.0	28.0	37.0	2.0	37.0
6	29.22952897047184	35.0	29.0	37.0	2.0	37.0
7	28.79764157977448	35.0	28.0	37.0	2.0	37.0
8	28.25056865376661	35.0	26.0	37.0	2.0	37.0
9	29.189582519404404	35.0	27.0	39.0	2.0	39.0
10	28.505685188146632	35.0	23.0	39.0	2.0	39.0
11	29.118452047187297	35.0	27.0	39.0	2.0	39.0
12	28.644290284337004	35.0	25.0	39.0	2.0	39.0
13	28.2683998545218	35.0	22.0	39.0	2.0	39.0
14	28.53004131553857	35.0	18.0	40.0	2.0	41.0
15	29.187698590224485	36.0	25.0	40.0	2.0	41.0
16	29.537424536558145	36.0	27.0	40.0	2.0	41.0
17	29.710224431835435	36.0	27.0	40.0	2.0	41.0
18	29.548479800055194	36.0	27.0	40.0	2.0	41.0
19	29.55179826843157	37.0	26.0	40.0	2.0	41.0
20	29.410052165675108	37.0	25.0	40.0	2.0	41.0
21	29.331104601928867	37.0	25.0	40.0	2.0	41.0
22	29.37832361341935	37.0	26.0	40.0	2.0	41.0
23	29.385364056493582	37.0	27.0	40.0	2.0	41.0
24	29.244601753430647	37.0	26.0	40.0	2.0	41.0
25	29.063173030663105	36.0	25.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9373459511482274
1101	2	1.016244823348007
1101	3	1.7674379216699805
1101	4	1.080288216224318
1101	5	0.5818655740560814
1101	6	0.43194323648609156
1101	7	0.7051262948787382
1101	8	0.9813822387184636
1101	9	0.8890184157341885
1101	10	1.190197027505974
1101	11	0.6165839793811472
1101	12	0.7526361090259925
1101	13	0.8078924707899517
1101	14	1.3003577096181438
1101	15	0.5470537060675333
1101	16	0.2070883667973753
1101	17	-0.6812752850478887
1101	18	-6.241369295605654
1101	19	-7.441795864955605
1101	20	-8.377028774583756
1101	21	-9.74926081130548
1101	22	-10.070986658186222
1101	23	-11.220989974264096
1101	24	-11.086817871307687
1101	25	-11.54541589467232
1102	1	1.7587930108494696
1102	2	2.055132621066292
1102	3	2.70613874857429
1102	4	1.9633654162907028
1102	5	1.528291888827841
1102	6	1.4493980962808202
1102	7	1.6593822028062704
1102	8	1.8237375228516761
1102	9	2.039520024322666
1102	10	-1.195294006878079
1102	11	0.6273764813642835
1102	12	1.7709744363680358
1102	13	2.128492794791491
1102	14	2.7107803428310397
1102	15	2.1993761104391183
1102	16	1.8001402237921091
1102	17	1.5170624180430679
1102	18	1.8455787941098833
1102	19	1.499023228601363
1102	20	1.9430837580942644
1102	21	2.005175939117411
1102	22	1.8300246285779806
1102	23	1.9251384509331118
1102	24	2.159932787581724
1102	25	2.174613962800496
1103	1	-0.8153185207526015
1103	2	-0.5957003860240526
1103	3	-2.959070699422579
1103	4	-1.1046834331590354
1103	5	-3.9691851654154924
1103	6	-7.637203104293366
1103	7	-8.831075189859789
1103	8	-3.0018747264849637
1103	9	-7.389750496361142
1103	10	-6.39602044125974
1103	11	-8.541340078729672
1103	12	-3.378646034291844
1103	13	-1.2124474374935161
1103	14	-1.050094423361486
1103	15	-1.5859614423034998
1103	16	-1.637384244431825
1103	17	-1.7539530676239892
1103	18	-1.2190010049244364
1103	19	-7.167132324054258
1103	20	-2.9152941617804586
1103	21	-7.210786042699979
1103	22	-3.60498776865548
1103	23	-2.676964728848521
1103	24	-8.952615605127832
1103	25	-5.82541866149808
1104	1	1.3523904830377163
1104	2	1.5224734679414773
1104	3	2.232354602730034
1104	4	1.7117025355172153
1104	5	1.320436160480238
1104	6	1.1426402769552908
1104	7	1.4048595165694486
1104	8	1.6860212901066127
1104	9	1.6403485524838857
1104	10	2.1539080433286877
1104	11	1.7225392747710053
1104	12	1.9151106702779899
1104	13	2.2528783152061926
1104	14	2.7387187783834896
1104	15	1.6411840200436352
1104	16	0.8881360301873578
1104	17	0.7587713119798138
1104	18	0.7134991738640934
1104	19	1.1254837097010189
1104	20	-8.955174731043133
1104	21	-3.5672671268736167
1104	22	-1.183871055503797
1104	23	0.0459666878717826
1104	24	0.46167138621880355
1104	25	0.9508400097455727
1105	1	1.2850768644529111
1105	2	1.330305043145934
1105	3	2.1198901603927105
1105	4	1.4071615256795944
1105	5	1.3363882168983956
1105	6	1.0954582217223887
1105	7	1.25109633060287
1105	8	1.4783541690920323
1105	9	1.4067865636252854
1105	10	2.188164888178754
1105	11	1.7168737538512495
1105	12	1.7897891697446262
1105	13	1.9149056946064533
1105	14	2.5800249298591353
1105	15	1.9422374158004239
1105	16	1.3505826936933403
1105	17	0.7403835556683305
1105	18	1.1958344233603597
1105	19	1.1891200733373815
1105	20	1.3687229814138995
1105	21	1.3060749971040977
1105	22	1.3299119834911082
1105	23	1.271098547537541
1105	24	1.2125425489543744
1105	25	1.2632866732515566
1106	1	1.002334132228313
1106	2	1.1925585064356667
1106	3	1.9113628284845525
1106	4	1.5381588746173591
1106	5	0.8152423722421887
1106	6	0.554473946929825
1106	7	0.8037054227197338
1106	8	0.8900712300966589
1106	9	1.1860622318258258
1106	10	-2.1710253602601988
1106	11	0.15523957628442986
1106	12	0.7447716872271428
1106	13	1.4183433191580654
1106	14	0.566414835290491
1106	15	1.2587457132025008
1106	16	0.8645287001911619
1106	17	-5.485058640304953
1106	18	-1.5982832681018913
1106	19	-0.2293522143058908
1106	20	0.5734631893924487
1106	21	0.7526876461131202
1106	22	0.9139722453363426
1106	23	0.414157021878399
1106	24	0.8249635907795607
1106	25	0.8055141996112098
1107	1	0.7209254470080815
1107	2	1.1499150738338066
1107	3	1.6773039292585779
1107	4	1.0876804827704092
1107	5	0.49130253910313826
1107	6	0.8081250459161602
1107	7	0.7557421339013679
1107	8	0.9252384973386363
1107	9	0.9906207683427262
1107	10	-2.1695619658055847
1107	11	-0.0280736640003596
1107	12	0.46825329543928973
1107	13	1.314301957026668
1107	14	1.7444809827882395
1107	15	1.4282398116082078
1107	16	-5.049133981846687
1107	17	-1.678720169947372
1107	18	0.05158982065767148
1107	19	0.6815572768524305
1107	20	1.0759042731760289
1107	21	1.2045496511925258
1107	22	0.9569769095829805
1107	23	0.6453526616868643
1107	24	0.9525577732510335
1107	25	1.221247499850108
1108	1	-0.1973819311684295
1108	2	-2.787556777012707
1108	3	0.1298406780729131
1108	4	-0.23767310082464377
1108	5	-0.47918203440507057
1108	6	-0.3218488528285022
1108	7	-0.06749629585194583
1108	8	0.16031906815057084
1108	9	-0.19674793191450846
1108	10	-3.0328530514787637
1108	11	-1.4234929465861228
1108	12	-6.013178842808191
1108	13	-1.81898938557838
1108	14	-5.658861625281837
1108	15	-2.4973258919165957
1108	16	-7.295416404381729
1108	17	-3.1670656647225854
1108	18	-1.7951386053776872
1108	19	-0.5870604599102904
1108	20	-0.6122253988277677
1108	21	0.02723038764995067
1108	22	-0.12068754948474947
1108	23	-0.3007048698930852
1108	24	0.03263323565283471
1108	25	-0.1989668417846424
1109	1	-8.098941771778115
1109	2	-6.00251331843349
1109	3	-5.001350140604227
1109	4	-4.704388981335516
1109	5	-2.158461144654506
1109	6	-1.4253738223397434
1109	7	-0.9875613980991069
1109	8	-0.6046688182451128
1109	9	-5.389670482278664
1109	10	-1.4625814893375377
1109	11	-1.1676049680168532
1109	12	-6.042522876616093
1109	13	-7.612384493081965
1109	14	-7.968079988821007
1109	15	-3.9932597934799716
1109	16	-2.1727691388399073
1109	17	-1.6665990266461108
1109	18	-1.0912345180227412
1109	19	-0.7144446510238041
1109	20	-0.6300706291053757
1109	21	-0.44631549588373787
1109	22	-0.7192105385296443
1109	23	-0.9406964789698762
1109	24	-0.7462166122384168
1109	25	-0.7088114719660545
1110	1	-2.6585089614858575
1110	2	-2.496914795838613
1110	3	-1.7337613351705485
1110	4	-2.5322762424346443
1110	5	-5.578641606219662
1110	6	-4.135283095783635
1110	7	-8.636035655058528
1110	8	-9.675586742937114
1110	9	-11.289764679249487
1110	10	-9.071159610851627
1110	11	-11.681993555310179
1110	12	-6.811110665714033
1110	13	-5.0028544863890225
1110	14	-4.749926001562386
1110	15	-10.03934937975696
1110	16	-11.250505189012188
1110	17	-12.142620939836164
1110	18	-8.883581014143314
1110	19	-11.586280996181333
1110	20	-12.848857967049401
1110	21	-14.320325807973177
1110	22	-14.650716946402529
1110	23	-15.409787478201427
1110	24	-15.672244722597604
1110	25	-15.961540705252292
1111	1	-0.21783863098714562
1111	2	0.6092897278333069
1111	3	-2.3971250488747415
1111	4	-2.6345230018816963
1111	5	-0.5345553685635593
1111	6	-0.02257341558519954
1111	7	0.4135369975821952
1111	8	0.6124611716580297
1111	9	-3.8683930037104126
1111	10	-3.6948198652139475
1111	11	-0.7788922806268737
1111	12	-4.849952789492207
1111	13	-0.8739214009805742
1111	14	0.5690160220646625
1111	15	0.6637965860193518
1111	16	0.4599505524471468
1111	17	0.4413419575825195
1111	18	0.7911789282787645
1111	19	0.8800544059846587
1111	20	0.954102414027421
1111	21	1.1130684895151468
1111	22	0.675158658004122
1111	23	0.43783348389549914
1111	24	0.8082046292918044
1111	25	1.052759188135223
1112	1	0.11639746126023454
1112	2	0.6279123031425726
1112	3	-2.1416788384550465
1112	4	-0.1047261073363579
1112	5	-0.1681535171787374
1112	6	0.01752546126750687
1112	7	0.6019876958747687
1112	8	-2.956544081624802
1112	9	-0.28853268202581717
1112	10	-2.4419325217859154
1112	11	-0.5241157139081309
1112	12	0.6013877849936833
1112	13	1.1680621988691513
1112	14	1.8949625733365991
1112	15	1.217443957046786
1112	16	0.8360804186822186
1112	17	0.5335695363543103
1112	18	0.5278131010248757
1112	19	0.647544392381171
1112	20	0.7277043392890619
1112	21	0.7563815121230029
1112	22	0.5989521247657628
1112	23	0.4090717469045444
1112	24	0.6532776987603199
1112	25	0.7492584816791172
1113	1	-1.3396046555774461
1113	2	-0.8438169158597475
1113	3	-3.0050371840113783
1113	4	-1.6380080441992781
1113	5	-2.125761229262679
1113	6	-2.072489140992417
1113	7	-1.9966788270560087
1113	8	-8.870571506868526
1113	9	-9.527148418274813
1113	10	-4.0544227352228255
1113	11	-9.265704714192335
1113	12	-7.8109991999390935
1113	13	-3.544570552660492
1113	14	-1.8261168396547305
1113	15	-7.153444733660798
1113	16	-3.3301087617074145
1113	17	-2.3804208660081834
1113	18	-1.9248743790344918
1113	19	-1.5468246451654757
1113	20	-6.37238593120664
1113	21	-2.664454312088001
1113	22	-2.243743097431416
1113	23	-2.27327519887109
1113	24	-7.6574204983342575
1113	25	-4.839777736498899
1114	1	-4.166823228668555
1114	2	-4.14936096514603
1114	3	-0.818563296667616
1114	4	-0.6312292078447257
1114	5	-0.5554414864771076
1114	6	-0.5458899339387422
1114	7	-0.4008940666165337
1114	8	0.003944407945631667
1114	9	-0.17293942497474646
1114	10	-2.8872004801255464
1114	11	-1.082261906925634
1114	12	-4.452641374689737
1114	13	-1.2047279584241473
1114	14	-5.8068717824192575
1114	15	-2.068117528237728
1114	16	-1.2498213022259215
1114	17	-0.9868472817724303
1114	18	-0.6302645730630658
1114	19	-0.33630594254664814
1114	20	-0.13723723733257742
1114	21	0.13554371540869425
1114	22	-0.019236058882125207
1114	23	-0.4842837398103974
1114	24	-0.2673481074630004
1114	25	-0.15104862037762956
1115	1	0.6854062536068035
1115	2	0.7559985549321588
1115	3	1.4528858071300803
1115	4	0.4483438532857633
1115	5	0.0840726516689223
1115	6	-0.3588006816259188
1115	7	-2.3678745164308914
1115	8	-0.2384637811696244
1115	9	-0.2046326463038568
1115	10	0.7677942819637771
1115	11	-0.18052644528736295
1115	12	0.4782153958364148
1115	13	0.5811053889518405
1115	14	1.0400727462811226
1115	15	0.27898427820953486
1115	16	0.1582084288853025
1115	17	-0.22189009783048874
1115	18	-0.09541737120269289
1115	19	0.41944066334340135
1115	20	0.5148148861978576
1115	21	0.7206537957888592
1115	22	0.13862024542784468
1115	23	0.1632076801268063
1115	24	0.0762339072649354
1115	25	0.043180620364047684
1116	1	0.1685539202863353
1116	2	0.2909570056713342
1116	3	1.2000284338594618
1116	4	0.5186526053742533
1116	5	-0.27460575374378493
1116	6	-1.1809904544973122
1116	7	-4.3726983800985835
1116	8	-0.9629865987995245
1116	9	-1.2241834759105323
1116	10	-0.37331432785916263
1116	11	-1.8893904024753567
1116	12	-0.3103906774689449
1116	13	-6.16063231858768
1116	14	-1.4841982116954
1116	15	-1.17680346213929
1116	16	-0.765331419254494
1116	17	-0.3113612752709223
1116	18	-0.32418058452933707
1116	19	0.06382675716146835
1116	20	0.19152434740843916
1116	21	0.2896074409300482
1116	22	0.14194253204330565
1116	23	0.0247096845271102
1116	24	0.2737393662919061
1116	25	0.20659404240197432
1201	1	2.2438181231941705
1201	2	2.380105242946133
1201	3	2.992909640912192
1201	4	2.53042576144383
1201	5	2.0360060990162765
1201	6	1.8107479320120987
1201	7	2.1347077192142585
1201	8	2.3860939293317927
1201	9	2.631489360828084
1201	10	2.7742637892776543
1201	11	2.3402753522528457
1201	12	2.740925533380988
1201	13	2.9583672330679924
1201	14	3.5559240307582343
1201	15	2.9644127404757477
1201	16	2.531039736650385
1201	17	2.429686752471529
1201	18	2.3830447130706496
1201	19	2.458121072338379
1201	20	2.581355349046266
1201	21	2.540508064037166
1201	22	1.6833360594151827
1201	23	1.4061659801049728
1201	24	1.7409291623409864
1201	25	1.899073386538273
1202	1	1.8581979757419624
1202	2	2.239103835532511
1202	3	2.933769188489297
1202	4	2.261052386726945
1202	5	1.8775525762879468
1202	6	1.6661541712351315
1202	7	1.905721709464892
1202	8	2.0578036106597963
1202	9	2.2944057828284734
1202	10	-1.2150206667243602
1202	11	0.8562453025739423
1202	12	2.0698353004586956
1202	13	2.5607512612535466
1202	14	2.972178259989562
1202	15	2.585826492118507
1202	16	2.113198205831754
1202	17	1.812983082943429
1202	18	2.023679447293169
1202	19	2.215856907326124
1202	20	2.0370630171203494
1202	21	2.1677744193951263
1202	22	1.8531133898032017
1202	23	1.5759367860589855
1202	24	1.863695149534454
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2211	21	0.934839435313549
2211	22	0.8014882941458303
2211	23	0.6627484200682687
2211	24	0.7231086122190113
2211	25	0.7317100777493017
2212	1	1.122575663148151
2212	2	1.1973234330018308
2212	3	1.8587374590116355
2212	4	1.3329975631263835
2212	5	0.9559297000716498
2212	6	0.7693951376762804
2212	7	0.9517940766724848
2212	8	1.3138407823171256
2212	9	1.408248268856191
2212	10	1.9256267997514165
2212	11	1.0924291806394955
2212	12	1.4632437971705556
2212	13	1.702364496919742
2212	14	-3.5860003342865845
2212	15	-0.4730504592186584
2212	16	0.08308074333973536
2212	17	0.4991159812863657
2212	18	0.6601079731735204
2212	19	1.0669954316088592
2212	20	1.4079187290777142
2212	21	1.2573108570404443
2212	22	1.1605382589941016
2212	23	1.1522071822334823
2212	24	1.2375784467671487
2212	25	1.3398631384193749
2213	1	0.9191777229440135
2213	2	1.066083086241047
2213	3	1.5954523015036344
2213	4	1.0802604795712298
2213	5	0.6525396467693376
2213	6	0.30918981533203294
2213	7	0.40052921479185244
2213	8	-4.981513503885395
2213	9	-1.5284918774458376
2213	10	0.2330523178222812
2213	11	0.17053086909372794
2213	12	0.7972107116173852
2213	13	-7.721601497388544
2213	14	-1.9953758888343671
2213	15	-0.6805019383413722
2213	16	-0.026429217160032437
2213	17	0.09349984994316074
2213	18	0.5442901343160713
2213	19	0.8791422462863849
2213	20	1.0542437614468767
2213	21	0.9785059198399004
2213	22	0.7090289031568062
2213	23	0.7039038519891854
2213	24	0.8569552500743924
2213	25	0.9019251689022489
2214	1	-0.7542316268135956
2214	2	-0.4993847462733676
2214	3	-6.626439181440265
2214	4	-2.2836412373627724
2214	5	-1.4017316819754377
2214	6	-0.7007497588315736
2214	7	-0.23338572952513204
2214	8	-5.722645905722132
2214	9	-1.615653274493802
2214	10	0.11863676793403855
2214	11	-0.22449770270995373
2214	12	0.15484435577780786
2214	13	-5.834088901834651
2214	14	-1.3428723686437358
2214	15	-0.5545432812981979
2214	16	-0.42746820675864683
2214	17	-0.1817350755994731
2214	18	-0.11480016308670216
2214	19	0.17903639440024932
2214	20	0.3836417021763836
2214	21	0.35326738384533485
2214	22	0.27586033772064766
2214	23	0.23443448995604044
2214	24	0.4572854423145465
2214	25	0.622145340825405
2215	1	-0.04170465653329458
2215	2	0.25275466504068334
2215	3	-2.791340075667982
2215	4	-0.8556602446175141
2215	5	-0.40082917421850084
2215	6	-0.8780215968560725
2215	7	-0.11228242997071192
2215	8	0.1916978281046191
2215	9	0.3607580699241666
2215	10	1.0433808292454962
2215	11	0.19040746428384736
2215	12	0.10677333128797173
2215	13	0.8450686779445284
2215	14	1.0645892249967943
2215	15	0.4658680956425698
2215	16	0.24348898078890002
2215	17	-0.015722930324098883
2215	18	0.1652930114225235
2215	19	0.20269291644166643
2215	20	0.43881364906930287
2215	21	0.5959795797799536
2215	22	0.28236805085161265
2215	23	0.3754215005812078
2215	24	0.23171691137780925
2215	25	0.1909751222871101
2216	1	0.1722920686035998
2216	2	-0.07424605092129255
2216	3	0.9673805732057055
2216	4	-0.4846752665604228
2216	5	-0.6194148510014443
2216	6	-0.9032249499446543
2216	7	-1.4564239716754948
2216	8	-6.007028190483876
2216	9	-2.1713518302389154
2216	10	-0.8702718363870048
2216	11	-1.57130298876098
2216	12	-0.28708315148253405
2216	13	0.28519105440794235
2216	14	0.9444254486951351
2216	15	0.5141610566756754
2216	16	-0.2210561513326006
2216	17	-0.1573098799283308
2216	18	0.07345680098273633
2216	19	0.20532241937843665
2216	20	0.25619760356594057
2216	21	0.011088531956342962
2216	22	-0.14982239503359196
2216	23	-0.37144036516266254
2216	24	-0.21789395003051482
2216	25	-0.10593281693567391
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	166335.0
3	1.0
4	1093.0
5	7465.0
6	12364.0
7	11100.0
8	9345.0
9	7403.0
10	6468.0
11	6048.0
12	5802.0
13	5399.0
14	5339.0
15	5268.0
16	5311.0
17	5630.0
18	6388.0
19	8412.0
20	11664.0
21	14587.0
22	18158.0
23	20721.0
24	23261.0
25	26080.0
26	28309.0
27	30826.0
28	32941.0
29	37791.0
30	43026.0
31	48001.0
32	59311.0
33	73959.0
34	92066.0
35	114583.0
36	143662.0
37	169903.0
38	217989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.97650233785015	17.97802028167946	13.133531663549986	21.911945716920403
2	14.419829652398814	22.213367818115344	41.88601064333979	21.480791886146054
3	15.455303231905049	30.844247081710748	30.63074592685436	23.06970375952985
4	10.226214684215623	18.520865963381215	39.123486659854564	32.1294326925486
5	11.423161624287932	40.322641810976485	33.502360694637446	14.751835870098141
6	26.64885329824804	39.127686387256674	20.34209518240429	13.881365132091
7	25.802261649893023	34.918239061822085	22.640459667108203	16.63903962117669
8	25.22046416429377	34.76616144968598	21.810198781550117	18.203175604470132
9	27.075094132481954	18.186943758760936	20.507987131786827	34.22997497697028
10	13.444424418265863	31.96729198032857	32.92870665367525	21.65957694773032
11	31.26832117618313	26.742571865035664	24.047535732004384	17.94157122677682
12	22.158004884097444	29.441916060666774	30.095746178545706	18.304332876690076
13	26.199522722135214	25.126662723191025	27.38655852338946	21.287256031284304
14	20.41635560922025	24.04007615633843	30.254344031453407	25.28922420298791
15	22.197030966069633	31.287269429508285	25.31603934799013	21.199660256431947
16	20.807422728417155	31.1364479301747	26.54854499037574	21.507584351032406
17	20.585566044695057	30.343060056997334	27.381932887704696	21.689441010602913
18	19.67062670801316	29.156249957701508	30.471969972807145	20.701153361478188
19	21.429528593414357	28.37630230011546	30.289162399548452	19.905006706921732
20	21.959825576269694	28.046888245386793	29.9265548600609	20.066731318282617
21	22.962384938003062	27.186880106769685	29.706552569653073	20.14418238557418
22	22.185817498857887	27.608805265562342	29.51334156782457	20.6920356677552
23	21.639777396228894	27.889561675226705	29.75691034736665	20.71375058117776
24	21.30034827736035	27.94380998918486	29.863599263108455	20.892242470346343
25	21.15220243940238	27.968466294952076	30.273805084671206	20.60552618097434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8899.0
1	8899.0
2	8261.5
3	7624.0
4	7624.0
5	7624.0
6	12917.0
7	18210.0
8	18210.0
9	18210.0
10	16651.0
11	15092.0
12	15092.0
13	15092.0
14	16323.0
15	17554.0
16	17554.0
17	17554.0
18	25250.5
19	32947.0
20	32947.0
21	32947.0
22	43450.5
23	53954.0
24	53954.0
25	53954.0
26	72074.5
27	90195.0
28	90195.0
29	90195.0
30	104361.5
31	118528.0
32	118528.0
33	118528.0
34	127969.0
35	137410.0
36	137410.0
37	137410.0
38	145418.0
39	153426.0
40	153426.0
41	153426.0
42	161798.0
43	170170.0
44	170170.0
45	170170.0
46	173765.5
47	177361.0
48	177361.0
49	177361.0
50	168605.0
51	159849.0
52	159849.0
53	159849.0
54	142457.0
55	125065.0
56	125065.0
57	125065.0
58	106070.0
59	87075.0
60	87075.0
61	87075.0
62	70236.0
63	53397.0
64	53397.0
65	53397.0
66	40837.0
67	28277.0
68	28277.0
69	28277.0
70	21292.0
71	14307.0
72	14307.0
73	14307.0
74	10568.0
75	6829.0
76	6829.0
77	6829.0
78	5189.5
79	3550.0
80	3550.0
81	3550.0
82	2455.5
83	1361.0
84	1361.0
85	1361.0
86	987.0
87	613.0
88	613.0
89	613.0
90	425.5
91	238.0
92	238.0
93	238.0
94	145.5
95	53.0
96	53.0
97	53.0
98	39.0
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.338122103172113
2	0.3393366706949823
3	0.35101001410922605
4	0.3526294374730518
5	0.3593095588488329
6	0.3014151735920632
7	0.3098496702786555
8	0.30755548717990244
9	0.3083651988618153
10	0.4158544246357478
11	0.307150631338946
12	0.30775791510038064
13	0.30114526969809224
14	0.3051263521341638
15	0.29851370673187544
16	0.3031695489028744
17	0.29662437947407877
18	0.2979064229704408
19	0.2989860385463246
20	0.5019537668124822
21	0.2993234184137883
22	0.30256226514143975
23	0.2972991392090062
24	0.3003355580161794
25	0.2936554366403983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1482009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.14090019481273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02908126935036	56.46264515938459
2	6.930058910234095	8.890004338109955
3	1.98508250023994	3.819749355790779
4	0.82907788204145	2.127112067429895
5	0.47256694513534087	1.5155434631646723
6	0.30855213955530375	1.1874487192867602
7	0.19854216609518738	0.8914271281981341
8	0.15796263284399986	0.8105492374205474
9	0.11851316708284851	0.6841387099468935
>10	0.8333537077297714	10.477399750145288
>50	0.0862866528858742	3.820254265846378
>100	0.04757762318895627	5.220334262734476
>500	0.001941911777590548	0.8177791376174063
>1k	0.0010788398764391934	1.6224798984358881
>5k	3.23651962931758E-4	1.6531345064883938
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9205	0.6211163360006586	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8743	0.5899424362470134	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5942	0.4009422344938526	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4082	0.2754369237973589	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4011	0.27064612967937446	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3972	0.2680145667131576	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2838	0.19149681277239206	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1880	0.12685483016634852	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1834	0.12375093538568255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	1.3495194698547714E-4	0.0	0.0	6.747597349273857E-5	0.0
13	1.3495194698547714E-4	0.0	0.0	6.747597349273857E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	155	0.0	16.537329	2
TATAACG	310	0.0	15.924834	2
TCACGCA	175	0.0	15.745665	4
ATCACGC	175	0.0	15.732335	3
ATAACGC	315	0.0	15.672059	3
TAACGCA	335	0.0	15.032532	4
GTTCGCG	70	4.384292E-7	14.943896	16
TTCGCGC	70	4.384292E-7	14.943896	17
ATCAGCG	40	0.005272693	14.2525425	4
GTCAACG	55	1.9711944E-4	13.808947	3
GTATAAC	430	0.0	13.688057	1
GGTATCA	2155	0.0	13.656298	1
TCGTTCG	65	5.3981727E-5	13.167795	14
TCGCGCA	80	1.9770596E-6	13.075465	18
TTGACCG	120	7.2941475E-10	12.679238	18
GTCTAAT	105	2.0127118E-8	12.657773	1
GTTATCA	350	0.0	12.476947	1
CGTCTCG	70	1.08043E-4	12.226824	17
GTATCAC	290	0.0	12.112179	1
CTCAACG	110	3.848436E-8	12.0828285	3
>>END_MODULE