FastQCFastQC Report
Thu 26 May 2016
SRR1364330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482009
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT201331.3584937743293057No Hit
GTACTTTTTTTTTTTTTTTTTTTTT191721.293649363802784No Hit
TATCAACGCAGAGTACTTTTTTTTT150061.012544458232035No Hit
GGTATCAACGCAGAGTACTTTTTTT136190.9189552829976065No Hit
GAGTACTTTTTTTTTTTTTTTTTTT84000.5667981773390041No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55460.37422174899072813No Hit
GTATAACGCAGAGTACTTTTTTTTT29770.20087597308788271No Hit
GTGGTATCAACGCAGAGTACTTTTT29540.19932402569754976No Hit
ATCAACGCAGAGTACTTTTTTTTTT22780.15371026761645845No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22390.15107870465024167No Hit
GTGGTATCAACGCAGAGTACATGGG21400.14439858327446056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA19480.13144319636385474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG5050.016.5540432
ATAACGC5000.016.531823
AACGATG350.00217490416.28036317
TAACGCA5200.015.8981294
TATCACG2400.015.8329392
GTATAAC6100.015.1072011
CGAGTGG400.005273910714.2520510
CCTAGAG707.2638595E-613.5710922
ATCACGC2850.013.3348033
GGTATCA33950.013.3201561
CCGATCA655.425848E-513.16062719
TCGTGAT655.453422E-513.153522
CGCGGTT655.470719E-513.1490818
TCACGCA2900.012.77899554
CACGTCA604.0868606E-412.66848910
TACAACG1352.7284841E-1112.6663512
CTTCGCG604.1020178E-412.66293115
ACAACGC1457.2759576E-1212.4496483
GTATCAC3300.012.3793151
CACGCAG3100.012.2631425
Produced by FastQC (version 0.11.5)