##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364330_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1482009
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.43454729357244	31.0	30.0	34.0	26.0	34.0
2	29.448682835259437	31.0	30.0	34.0	25.0	34.0
3	29.47758077042717	31.0	30.0	34.0	25.0	34.0
4	32.78345003302949	35.0	33.0	37.0	26.0	37.0
5	32.374549682221904	35.0	32.0	37.0	24.0	37.0
6	32.239288020518096	35.0	32.0	37.0	22.0	37.0
7	31.957611593451862	35.0	32.0	37.0	19.0	37.0
8	31.818482208947447	35.0	32.0	37.0	17.0	37.0
9	32.51469390536764	37.0	32.0	39.0	11.0	39.0
10	32.64258314220764	37.0	32.0	39.0	16.0	39.0
11	32.55824154914039	37.0	32.0	39.0	11.0	39.0
12	32.493810091571646	37.0	32.0	39.0	11.0	39.0
13	32.53815800039001	37.0	32.0	39.0	11.0	39.0
14	33.43321126929729	38.0	32.0	41.0	11.0	41.0
15	33.46643643864511	38.0	32.0	41.0	11.0	41.0
16	33.4341194959005	38.0	32.0	41.0	10.0	41.0
17	33.437155914707674	38.0	32.0	41.0	10.0	41.0
18	33.402636556188256	38.0	32.0	41.0	10.0	41.0
19	33.33182726960498	39.0	32.0	41.0	10.0	41.0
20	33.20768294929383	39.0	32.0	41.0	7.0	41.0
21	33.133752898936514	38.0	32.0	41.0	2.0	41.0
22	33.08497249341941	38.0	32.0	41.0	2.0	41.0
23	32.788001287441574	38.0	32.0	41.0	2.0	41.0
24	32.70830136659089	38.0	32.0	41.0	2.0	41.0
25	32.555878540548676	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.29962855104992414
1101	2	0.4531540616601397
1101	3	-1.3352776594739169
1101	4	-0.5539632583081939
1101	5	0.48815766417840933
1101	6	0.8782778873588271
1101	7	1.1460154188848932
1101	8	1.0666845396547728
1101	9	1.7230226317707817
1101	10	1.4581960958928732
1101	11	1.3163000192741237
1101	12	1.5637431180485564
1101	13	1.3417698725565046
1101	14	1.4073642115450227
1101	15	1.348696086027708
1101	16	1.4607361950571018
1101	17	1.3752849981523951
1101	18	1.4205339066604523
1101	19	1.5676080410079223
1101	20	1.7135816511094575
1101	21	1.6973838419186578
1101	22	1.7407532649884985
1101	23	1.9174159494330922
1101	24	1.9707704280073415
1101	25	2.0684864408329062
1102	1	0.5724740473828298
1102	2	0.5825350481900209
1102	3	0.6606500537725388
1102	4	0.6533984891076301
1102	5	0.7279374558003511
1102	6	0.856862839557941
1102	7	0.8732093188859551
1102	8	0.9284553648994844
1102	9	1.48185483307806
1102	10	0.9962059453570049
1102	11	1.1391898983368947
1102	12	1.3599131472970072
1102	13	1.1701522096003245
1102	14	1.1334211371540874
1102	15	1.2498436422369466
1102	16	1.1620126362777725
1102	17	1.0932713849109632
1102	18	1.1833531311578938
1102	19	1.3262255608431204
1102	20	1.4038284510613437
1102	21	1.5339092299059374
1102	22	1.5155097689996921
1102	23	2.0126157630713024
1102	24	1.9137932421007946
1102	25	1.7950201191791564
1103	1	-2.09535078188334
1103	2	-1.916540596683614
1103	3	-1.8611484150603808
1103	4	-1.750555575879968
1103	5	-4.3918480938103315
1103	6	-8.997246734586213
1103	7	-9.75111100822701
1103	8	-9.735954787782234
1103	9	-10.183196930809018
1103	10	-8.946902715306791
1103	11	-11.434459455701994
1103	12	-11.406621934698734
1103	13	-11.756562741028237
1103	14	-11.038541540648342
1103	15	-11.191236874531942
1103	16	-5.868469333359883
1103	17	-4.059317896919477
1103	18	-3.63896406402241
1103	19	-2.9474216182425295
1103	20	-7.0160190847166355
1103	21	-4.004870186336614
1103	22	-9.111920741244337
1103	23	-10.376744365685926
1103	24	-12.186379666681663
1103	25	-13.005455108103455
1104	1	0.33718515461097454
1104	2	0.629953360264107
1104	3	0.5992981986848811
1104	4	0.809876734782975
1104	5	0.8012774872523849
1104	6	0.8857200822628144
1104	7	0.73843880225715
1104	8	0.9098025604263071
1104	9	1.1355722889578388
1104	10	1.2314424180694488
1104	11	1.2507761875791346
1104	12	0.9831978464641082
1104	13	0.8932548946722605
1104	14	0.9538241891664327
1104	15	1.0149813905041754
1104	16	0.6554776022047335
1104	17	0.6249665913877536
1104	18	0.9791507897285712
1104	19	1.1576077030762164
1104	20	1.2660233954378342
1104	21	1.1981441882611108
1104	22	0.9581214528484878
1104	23	1.2478551480092719
1104	24	1.4456020253729776
1104	25	1.4886234158184521
1105	1	0.4670772963675205
1105	2	0.3836128363812108
1105	3	0.0730136781206987
1105	4	0.3215100167552407
1105	5	0.38173855161175396
1105	6	0.5995949785529646
1105	7	0.7911826685603387
1105	8	0.5169718741634171
1105	9	0.7072846867295581
1105	10	0.4657437099978594
1105	11	0.7779881705114562
1105	12	0.9139167038778808
1105	13	0.8770346742147481
1105	14	0.7959259433444572
1105	15	0.7364448846371587
1105	16	0.7342496775869805
1105	17	0.8055743311056602
1105	18	0.8686227584378301
1105	19	0.8565676414429362
1105	20	1.0162737806458466
1105	21	1.034786359649047
1105	22	0.7988557185827574
1105	23	1.3481899826661419
1105	24	1.3337731762559883
1105	25	1.4698978617115372
1106	1	-0.3824673731253334
1106	2	-0.07564293375538966
1106	3	-0.23958898260372408
1106	4	-1.4677622394859249
1106	5	-0.6295888515550239
1106	6	-0.10764003600069927
1106	7	0.1780007296011945
1106	8	0.16712450650516786
1106	9	0.5092015738148419
1106	10	0.24110694779633235
1106	11	0.25355567295719794
1106	12	0.3861515070712329
1106	13	0.07723516457068058
1106	14	-0.25973806018224366
1106	15	0.2114598308308615
1106	16	-0.02806357047756336
1106	17	-0.1868129232634601
1106	18	-0.18799010193150423
1106	19	-1.2697473216595938
1106	20	-0.22415839080964162
1106	21	-0.41194480210802453
1106	22	5.221436947806524E-4
1106	23	-0.1860292523302931
1106	24	-0.12291909520312316
1106	25	0.3127386349279604
1107	1	-0.01497807341240076
1107	2	-0.20562427381398507
1107	3	-0.25033026835199124
1107	4	-0.030373877793323345
1107	5	0.15438132799705073
1107	6	-0.008980951504995005
1107	7	-0.06929742021316443
1107	8	-0.2878112917724458
1107	9	0.5404750741864177
1107	10	0.2484191184217721
1107	11	0.24702026117528675
1107	12	0.019605177081324143
1107	13	0.2206925133815716
1107	14	0.17546444376618808
1107	15	0.10310031005006692
1107	16	-0.06246109666870581
1107	17	-0.3868129971349603
1107	18	-0.1652243824452384
1107	19	0.004751792621100037
1107	20	0.16481274168481264
1107	21	0.1624371519704937
1107	22	0.15683830485875916
1107	23	0.3625094954886521
1107	24	0.5838195370807924
1107	25	0.13076431561691493
1108	1	-0.11588126450623548
1108	2	-0.05033351761814586
1108	3	0.13175462798355753
1108	4	-0.08581410788337251
1108	5	0.13996707220776727
1108	6	0.09263749257604559
1108	7	-0.05960126482077044
1108	8	0.1201696922463924
1108	9	0.6057301017385797
1108	10	0.3829920976526111
1108	11	0.4560241363124433
1108	12	0.5057153645811354
1108	13	0.25140456227721586
1108	14	0.3232168944428295
1108	15	-0.04673328877952798
1108	16	-0.6560768686582961
1108	17	-0.4334960066837539
1108	18	-0.5172497612686087
1108	19	-0.35404109610927037
1108	20	0.0798177896403871
1108	21	0.02852539446581659
1108	22	0.21333346698276756
1108	23	-0.37710592284177835
1108	24	0.11293747485491679
1108	25	-0.09270406816070675
1109	1	0.23088796947023127
1109	2	0.2054915476404524
1109	3	0.2359033327138924
1109	4	0.23263613252737514
1109	5	0.20501962157424458
1109	6	0.522247363041636
1109	7	0.5143330432593842
1109	8	0.31937761738774384
1109	9	1.1450421726065159
1109	10	0.6041009280010954
1109	11	0.6326510224088224
1109	12	0.6956147256201533
1109	13	0.6999602736890225
1109	14	0.24902907177944655
1109	15	0.08048561529293607
1109	16	0.5207618379929428
1109	17	0.4240898495105583
1109	18	0.4120155750607353
1109	19	0.5970955501124351
1109	20	0.8925834960042351
1109	21	0.8574415805343563
1109	22	0.5959638464430981
1109	23	0.8751239631399486
1109	24	0.561853355663601
1109	25	0.6853971485603836
1110	1	-0.017529273533167355
1110	2	-8.791422284168959E-4
1110	3	-0.11310388079097677
1110	4	-0.027286774688604964
1110	5	0.027573598917392417
1110	6	0.13518953105928233
1110	7	0.2579908603685297
1110	8	0.0018656568513151228
1110	9	-3.124077333603047
1110	10	-1.3888752005034029
1110	11	-0.503852782653027
1110	12	-0.0398045748434015
1110	13	-0.2572222453362869
1110	14	0.17238992054372915
1110	15	-0.05118439784506279
1110	16	-0.39851755518025556
1110	17	-0.19709579288799262
1110	18	-0.12037207026288144
1110	19	-0.2675347267770789
1110	20	0.12909845597052083
1110	21	-0.055338221652007746
1110	22	0.314423973856897
1110	23	0.45096367955748917
1110	24	0.5277057143061725
1110	25	0.5848703891840685
1111	1	-0.06456225161119633
1111	2	-0.09394143335588723
1111	3	-0.07926177496103293
1111	4	-0.08977012770516524
1111	5	0.06413288730596634
1111	6	0.07850076413858886
1111	7	0.24204529376085304
1111	8	0.14019356383640869
1111	9	0.9026025309955017
1111	10	0.3274219719324236
1111	11	0.4547130101741459
1111	12	0.6775842089486304
1111	13	0.398991252450692
1111	14	0.2595152961104432
1111	15	0.4354003770302839
1111	16	0.18197612883756165
1111	17	0.3248952760726951
1111	18	0.1579509548036171
1111	19	0.2372518867149651
1111	20	0.4491234758033187
1111	21	0.4673341621242315
1111	22	0.4443239304221791
1111	23	0.7424607055357555
1111	24	0.6381750964204045
1111	25	0.9560457110855722
1112	1	-0.2840719968171683
1112	2	-0.3124182711543213
1112	3	-0.22553788093970795
1112	4	-0.167279585972409
1112	5	-0.35250250231956315
1112	6	-0.19498820375145343
1112	7	-0.07943012572502539
1112	8	-0.4083931369316822
1112	9	0.20193218524874368
1112	10	0.05542836162941711
1112	11	0.07765734352133791
1112	12	-0.4965470948602544
1112	13	-0.1601324359612093
1112	14	-1.0442705489538042
1112	15	-0.38432685836909997
1112	16	-0.3894783642826596
1112	17	-0.3593066949479322
1112	18	-0.6444530300804345
1112	19	-0.19135788435870182
1112	20	-0.29410002696247517
1112	21	-0.479263012807408
1112	22	-0.2165098155880898
1112	23	-0.005617714061905588
1112	24	-0.1027062734977946
1112	25	-0.12033399345394713
1113	1	-2.255830071888685
1113	2	-2.2487182261824117
1113	3	-2.339555288227338
1113	4	-2.3534077657206787
1113	5	-2.1032492211667133
1113	6	-2.135124382619882
1113	7	-2.5523081429537307
1113	8	-2.6260757005702047
1113	9	-11.21617810155881
1113	10	-4.7145626330991774
1113	11	-3.2871944397292943
1113	12	-12.98703602556322
1113	13	-5.331612621641849
1113	14	-4.381556619312523
1113	15	-3.6696001362334414
1113	16	-3.5206351877630055
1113	17	-3.520138446642001
1113	18	-3.9195820384878957
1113	19	-4.843299244470465
1113	20	-15.184762747425562
1113	21	-18.170353892002836
1113	22	-12.841017220708789
1113	23	-18.701867840647495
1113	24	-19.912870361085222
1113	25	-16.594722326455162
1114	1	-0.32845435603436
1114	2	-0.2669770317659115
1114	3	-0.24164679923061883
1114	4	-0.8071525632432142
1114	5	-0.5057023577757178
1114	6	-0.1965842980652397
1114	7	-0.42057450979989497
1114	8	-0.2703971923087032
1114	9	0.37166075807127896
1114	10	-0.41090889947917475
1114	11	-0.5599949341701205
1114	12	-0.5972200601214794
1114	13	-0.3902847324176335
1114	14	-0.4921548795646089
1114	15	-0.42965497182529333
1114	16	-0.5056967627411453
1114	17	-0.5900669762722899
1114	18	-0.5451976238940048
1114	19	-0.8827723230878703
1114	20	-1.1090876581697877
1114	21	-1.2008257805432478
1114	22	-1.3372135768376836
1114	23	-1.219294907337833
1114	24	-0.7466968202445408
1114	25	-0.9929740310267654
1115	1	-0.48807020513759625
1115	2	-0.4139488734321546
1115	3	-0.43151614635945634
1115	4	-0.30860738240188823
1115	5	-0.625031582227912
1115	6	-0.6957929944012307
1115	7	-0.8233596599720592
1115	8	-0.4360134683086798
1115	9	-0.09642484876805213
1115	10	-0.356883993424546
1115	11	-0.2797070316373862
1115	12	-0.41311346793705894
1115	13	-0.47908768025197546
1115	14	-0.6987743067365884
1115	15	-0.7384659661015078
1115	16	-0.6034726166750488
1115	17	-0.5205730314655383
1115	18	-0.7143178936253349
1115	19	-0.5087619792674829
1115	20	-1.0194351296491533
1115	21	-0.4208155715323585
1115	22	-0.8534679511250189
1115	23	-0.5608719818593997
1115	24	-0.17015170680796388
1115	25	-0.22702648877241316
1116	1	-0.9262880809695453
1116	2	-0.8961918718165052
1116	3	-0.8786724895833693
1116	4	-0.7059197765987832
1116	5	-1.7907175310233434
1116	6	-1.442527917285581
1116	7	-2.7503117446035183
1116	8	-1.968161071575274
1116	9	-0.9920833370035744
1116	10	-1.8714083136654018
1116	11	-1.6748006622211733
1116	12	-2.479277633009211
1116	13	-2.268145915387155
1116	14	-1.8521743431301019
1116	15	-2.770820695328254
1116	16	-2.974400747757869
1116	17	-3.6972514754774295
1116	18	-2.9373001903868676
1116	19	-2.3646495260689875
1116	20	-2.9801259998401086
1116	21	-2.1772950643391233
1116	22	-1.9522032765436812
1116	23	-2.093386966612723
1116	24	-2.39317639374104
1116	25	-2.105988418952009
1201	1	0.8186956779859038
1201	2	0.9880284207967946
1201	3	0.6191650267720483
1201	4	0.9067597670049921
1201	5	1.1828224676956722
1201	6	1.0667603132183032
1201	7	1.3034853658630325
1201	8	1.3125637822553244
1201	9	2.0844100613549372
1201	10	1.7113381155922696
1201	11	1.643335323358606
1201	12	1.801623996585171
1201	13	1.5735082006882735
1201	14	1.677871497790015
1201	15	1.644109719801996
1201	16	1.4884055217428198
1201	17	1.6231692362005674
1201	18	1.4459304507455215
1201	19	1.6201640450393384
1201	20	1.7525050493771204
1201	21	1.8775835624462474
1201	22	1.7572423319693726
1201	23	2.067660512136669
1201	24	2.084842107324924
1201	25	2.1322330265467073
1202	1	0.8823174845084729
1202	2	0.9887481590737863
1202	3	0.9207789853325714
1202	4	1.0427202102936803
1202	5	1.0634240205109649
1202	6	0.9845013677532535
1202	7	1.1320124282832253
1202	8	1.1973721037048648
1202	9	1.827979672405462
1202	10	1.4839172243545846
1202	11	1.526943148951169
1202	12	1.7501081841533335
1202	13	1.6542445296688797
1202	14	1.5901182332428547
1202	15	1.5245435014571527
1202	16	1.4035483602444359
1202	17	1.4107812001484064
1202	18	1.281350298073768
1202	19	1.531561858421881
1202	20	1.7513873206258026
1202	21	1.7550690455805835
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2211	2	0.09683261049711334
2211	3	0.1221269121371904
2211	4	0.12652297731810336
2211	5	-0.5175503977281082
2211	6	0.08796127111987317
2211	7	0.24772027555619047
2211	8	0.16042305129585444
2211	9	0.7897111960654399
2211	10	0.07769876105678009
2211	11	0.6833299519479397
2211	12	0.5995756986509306
2211	13	0.5035831766288297
2211	14	0.3256716813132101
2211	15	0.3929837378409857
2211	16	0.37292556381887465
2211	17	0.28375874852124383
2211	18	0.2093520393402315
2211	19	-0.15083839447633807
2211	20	0.4802040713327571
2211	21	0.7281365939361422
2211	22	0.505982959976798
2211	23	0.833045351330064
2211	24	0.8353911102568148
2211	25	0.8816262809501438
2212	1	-0.1899191025448026
2212	2	-0.17973691007282255
2212	3	-0.22889364051054883
2212	4	-0.17337313162426682
2212	5	-0.3648773022191314
2212	6	-0.31549150125663417
2212	7	-0.16279675944900163
2212	8	-0.08254668528844888
2212	9	0.2682696294343927
2212	10	-0.15987884852687984
2212	11	0.1778187865405343
2212	12	0.07117244328802741
2212	13	-0.2613824866357177
2212	14	-0.30152637192313847
2212	15	-0.0663464529662221
2212	16	0.019584997077508604
2212	17	-0.10631809134657999
2212	18	-0.06144222508309127
2212	19	0.2785930695314178
2212	20	0.38695155679047843
2212	21	0.459242663248439
2212	22	0.15690884169247
2212	23	0.4082311944858503
2212	24	0.4804115015523607
2212	25	0.5050286060682367
2213	1	-0.08927894901311362
2213	2	-0.02813882658996647
2213	3	-0.16760616951134466
2213	4	-0.10729633949014072
2213	5	0.038238352623750416
2213	6	0.06729993619695307
2213	7	-0.12958172964308545
2213	8	0.09783136020583072
2213	9	0.5153159846998818
2213	10	0.22462141765605992
2213	11	0.21618889255363882
2213	12	0.07273897916356731
2213	13	-0.14441907528777875
2213	14	0.14160150761757961
2213	15	0.01848834930257226
2213	16	0.056070133553774326
2213	17	-0.03948152766970736
2213	18	-0.1433378419374023
2213	19	-0.16076421369380256
2213	20	0.05569543622603845
2213	21	-0.09309185969259204
2213	22	0.0024303959379210482
2213	23	-0.1659694351518155
2213	24	-0.19503460133227435
2213	25	0.14485877478220743
2214	1	-0.5781111647474653
2214	2	-0.41172242988663754
2214	3	-0.4794358152603664
2214	4	-0.20430475184313224
2214	5	-0.6283871220686201
2214	6	-0.49808567648033275
2214	7	-0.3016204205588622
2214	8	-0.4772404385541442
2214	9	-10.201428799017634
2214	10	-3.3233142243971727
2214	11	-1.3282707048481441
2214	12	-0.5880224651484909
2214	13	-0.5949135430907546
2214	14	-0.5541731148440263
2214	15	-0.7218075764665315
2214	16	-0.6740034540210189
2214	17	-0.5745185019797034
2214	18	-0.8950142404131327
2214	19	-1.0801226363858163
2214	20	-0.7406604689963174
2214	21	-1.3409698986263905
2214	22	-1.1297267506234512
2214	23	-1.5746801427675692
2214	24	-1.9444748171716562
2214	25	-9.343231000746147
2215	1	-0.8995202450142372
2215	2	-0.5773594014097689
2215	3	-0.4372356543388669
2215	4	-0.6657251586735171
2215	5	-0.711737587949461
2215	6	-0.6737985771309702
2215	7	-0.8872090691717212
2215	8	-0.7744341355687325
2215	9	0.030588207089188302
2215	10	-0.1901560827699953
2215	11	-0.1881312782682869
2215	12	-0.09969242640818976
2215	13	-0.11664848192216937
2215	14	-0.5385389392862692
2215	15	-0.8633507933798512
2215	16	-0.2439083132097295
2215	17	-0.5341809352063223
2215	18	-0.4299758377871541
2215	19	-0.593979756083499
2215	20	-0.41166544515095893
2215	21	-0.5923826078405128
2215	22	-0.6671970839373671
2215	23	-0.24918397506539947
2215	24	-0.19431382100486871
2215	25	0.02834699067546609
2216	1	-0.7218156655093111
2216	2	-0.61814669441058
2216	3	-0.692678291200302
2216	4	-0.6190325052388914
2216	5	-1.4924690955797608
2216	6	-1.8616897045059098
2216	7	-1.4860759599079714
2216	8	-2.252027674674842
2216	9	-0.9195876344975993
2216	10	-1.3157896759710077
2216	11	-0.8726519152092749
2216	12	-1.8391709342904043
2216	13	-1.6260247663446918
2216	14	-1.8828215188707453
2216	15	-1.5196345198293422
2216	16	-2.266720320157212
2216	17	-2.6044893954903223
2216	18	-2.060843742802863
2216	19	-3.7984543526919623
2216	20	-2.6711895624626543
2216	21	-2.9102142043031307
2216	22	-2.689862418119251
2216	23	-2.2447295977534374
2216	24	-2.226800927060051
2216	25	-2.1555673988343678
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60363.0
3	3.0
4	812.0
5	8486.0
6	11997.0
7	9433.0
8	7366.0
9	5746.0
10	5008.0
11	4423.0
12	3871.0
13	3598.0
14	3616.0
15	3588.0
16	3676.0
17	3930.0
18	4474.0
19	5721.0
20	8110.0
21	10389.0
22	12690.0
23	14471.0
24	16542.0
25	18552.0
26	20465.0
27	21909.0
28	24680.0
29	28419.0
30	34595.0
31	42367.0
32	52142.0
33	64191.0
34	80328.0
35	100604.0
36	132846.0
37	201420.0
38	451178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92587530582975	18.945347169492955	13.135644984392137	22.99313254028516
2	15.962717873248057	22.294444590643568	39.66006678825936	22.08277074784902
3	17.693033838571612	29.788455790019885	30.075903825015672	22.44260654639283
4	11.537921708454007	18.74411247898037	37.78027299203481	31.937692820530817
5	11.686665420189819	39.515858492928494	34.12608360799698	14.671392478884703
6	27.13283391020506	38.979500527383806	19.778006172043867	14.109659390367266
7	25.14238856437097	33.86306806102512	23.524298349514236	17.470245025089678
8	25.77941866312091	34.270325361015395	21.47578437956648	18.47447159629721
9	26.618052855538277	16.167838474166235	20.99067684033178	36.22343182996371
10	15.499465705589266	30.730130357035573	32.240533065837354	21.529870871537803
11	31.947938682617266	24.62733063904788	24.641506351731834	18.78322432660302
12	22.741508281681778	28.285123339530394	30.616478336713037	18.356890042074788
13	27.511420609054166	23.255059144247184	27.792735345519816	21.440784901178837
14	21.493849462481688	22.34302785000098	30.58013918589813	25.5829835016192
15	22.828542662567447	29.587531749609287	26.538152165991413	21.045773421831854
16	20.78479907305496	29.13398526308544	28.145495820446914	21.93571984341269
17	20.65633952039842	28.254383863468423	29.360160069642443	21.729116546490715
18	19.611508567718754	26.45346855781177	33.01428076850659	20.920742105962884
19	21.36381244440725	26.12909800385758	32.73510249311966	19.771987058615508
20	21.498260543871076	25.76237443353793	32.62916010082434	20.110204921766652
21	22.31553033447179	24.86764667178197	32.52048650687765	20.296336486868594
22	21.606525383238722	25.313798213577137	32.43560050855556	20.644075894628585
23	20.991319158584613	25.430735469173026	32.96708784223327	20.610857530009095
24	20.950925316733304	25.73463569343928	32.70178990164819	20.61264908817923
25	20.837087903551566	25.63593525612361	32.99230352508548	20.534673315239342
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2582.0
1	2582.0
2	3410.0
3	4238.0
4	4238.0
5	4238.0
6	13497.0
7	22756.0
8	22756.0
9	22756.0
10	18947.0
11	15138.0
12	15138.0
13	15138.0
14	13847.0
15	12556.0
16	12556.0
17	12556.0
18	20666.0
19	28776.0
20	28776.0
21	28776.0
22	40189.0
23	51602.0
24	51602.0
25	51602.0
26	72837.5
27	94073.0
28	94073.0
29	94073.0
30	106921.0
31	119769.0
32	119769.0
33	119769.0
34	129101.0
35	138433.0
36	138433.0
37	138433.0
38	142925.0
39	147417.0
40	147417.0
41	147417.0
42	154895.0
43	162373.0
44	162373.0
45	162373.0
46	169310.0
47	176247.0
48	176247.0
49	176247.0
50	170544.0
51	164841.0
52	164841.0
53	164841.0
54	147601.5
55	130362.0
56	130362.0
57	130362.0
58	111197.0
59	92032.0
60	92032.0
61	92032.0
62	75071.5
63	58111.0
64	58111.0
65	58111.0
66	44431.5
67	30752.0
68	30752.0
69	30752.0
70	23377.0
71	16002.0
72	16002.0
73	16002.0
74	11605.0
75	7208.0
76	7208.0
77	7208.0
78	5663.5
79	4119.0
80	4119.0
81	4119.0
82	2871.5
83	1624.0
84	1624.0
85	1624.0
86	1126.0
87	628.0
88	628.0
89	628.0
90	481.5
91	335.0
92	335.0
93	335.0
94	184.0
95	33.0
96	33.0
97	33.0
98	17.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02591077382121161
2	8.097116819128628E-4
3	1.3495194698547714E-4
4	0.004116034383057052
5	0.03373798674636928
6	0.011201011599794603
7	0.021862215411647297
8	0.028609812760921154
9	0.03623459776560062
10	0.04156519967152696
11	0.04062053604262862
12	0.008367020713099582
13	0.002631562966216804
14	0.007152453190230289
15	0.006815073322766596
16	0.010256347970896263
17	0.01039129991788174
18	0.015249570009358917
19	0.016936469346677384
20	0.01511461806237344
21	0.012145675228692943
22	0.011133535626301864
23	0.00843449668659232
24	0.00566798177339004
25	0.10735427382694708
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1482009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.6529156310337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67060584350108	37.96536658533697
2	11.352363425801167	10.819464330850712
3	3.5711712415434813	5.105301656317361
4	1.5005488149709196	2.8602210432022734
5	0.8237767640681105	1.9627682318471815
6	0.5211119015600035	1.4899500887619832
7	0.37603623307373524	1.25434560222121
8	0.26632928572926623	1.0153093586344153
9	0.21142695507086495	0.9067599766906452
>10	1.4153855679668765	13.472387824097689
>50	0.18004755833105152	6.0189476866012095
>100	0.10493183830925362	8.943621314628594
>500	0.003986544592602804	1.325451129671599
>1k	0.0014237659259295728	1.3050971504076
>5k	2.8475318518591457E-4	0.9461886493857554
>10k+	5.695063703718291E-4	4.608819371344784
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	20133	1.3584937743293057	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	19172	1.293649363802784	No Hit
TATCAACGCAGAGTACTTTTTTTTT	15006	1.012544458232035	No Hit
GGTATCAACGCAGAGTACTTTTTTT	13619	0.9189552829976065	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8400	0.5667981773390041	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5546	0.37422174899072813	No Hit
GTATAACGCAGAGTACTTTTTTTTT	2977	0.20087597308788271	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2954	0.19932402569754976	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2278	0.15371026761645845	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2239	0.15107870465024167	No Hit
GTGGTATCAACGCAGAGTACATGGG	2140	0.14439858327446056	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1948	0.13144319636385474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.747597349273857E-5	0.0	0.0	0.0	0.0
3	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
4	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
5	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
6	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
7	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
8	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
9	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
10	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
11	6.747597349273857E-5	0.0	0.0	6.747597349273857E-5	0.0
12	6.747597349273857E-5	0.0	0.0	1.3495194698547714E-4	0.0
13	6.747597349273857E-5	0.0	0.0	1.3495194698547714E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	505	0.0	16.554043	2
ATAACGC	500	0.0	16.53182	3
AACGATG	35	0.002174904	16.280363	17
TAACGCA	520	0.0	15.898129	4
TATCACG	240	0.0	15.832939	2
GTATAAC	610	0.0	15.107201	1
CGAGTGG	40	0.0052739107	14.25205	10
CCTAGAG	70	7.2638595E-6	13.571092	2
ATCACGC	285	0.0	13.334803	3
GGTATCA	3395	0.0	13.320156	1
CCGATCA	65	5.425848E-5	13.160627	19
TCGTGAT	65	5.453422E-5	13.15352	2
CGCGGTT	65	5.470719E-5	13.14908	18
TCACGCA	290	0.0	12.7789955	4
CACGTCA	60	4.0868606E-4	12.668489	10
TACAACG	135	2.7284841E-11	12.666351	2
CTTCGCG	60	4.1020178E-4	12.662931	15
ACAACGC	145	7.2759576E-12	12.449648	3
GTATCAC	330	0.0	12.379315	1
CACGCAG	310	0.0	12.263142	5
>>END_MODULE