FastQCFastQC Report
Thu 26 May 2016
SRR1364329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528240
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99620.6518609642464535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA85950.5624116630895671No Hit
TATCAACGCAGAGTACTTTTTTTTT59060.3864576244568916No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46770.30603831858870334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN42880.28058420143432966No Hit
GGTATCAACGCAGAGTACTTTTTTT42530.27829398523792076No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA36880.24132335235303357No Hit
GAACAAAAAAAAAAAAAAAAAAAAA27900.182562948228027No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23680.15494948437418204No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20860.13649688530597287No Hit
GTGGTATCAACGCAGAGTACATGGG16040.10495733654399832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC1950.015.5786423
TAACGCA2100.015.3876864
TCACGCA704.4138687E-714.9351064
CGCATAT1450.014.4120488
TATAACG1850.014.368142
ACGGCCC707.1928043E-613.58406815
CTCAACG1102.7757778E-912.9453213
GGTATCA20050.012.8312561
CGCGCCA604.0972547E-412.66471810
CGCCGAC604.1360263E-412.65058513
GTCGGTT905.4886186E-712.65058512
GGTCGGT905.492984E-712.64975511
GCCCGCT550.003047074212.1021715
CTGGTCG1103.821333E-812.0890489
TTGACCG951.0272724E-612.01196618
GTCAACG1459.822543E-1111.7847063
GTATCAA54300.011.7660171
TACAACG658.07134E-411.6839832
TCTTATA1900.011.4918112
GTATAGG2400.011.4709931
Produced by FastQC (version 0.11.5)